52results about How to "Strong detection signal" patented technology

The present invention relates to protein chip based on labeling streptavidin-biotin technology. The hybridization reaction includes the following steps: dropping tested sample to sample applied chip and washing out redundant sample with elution liquid; blocking wtih blockage liquid, washing and air drying; dropping protein marked with biotin diluted by blockage liquid, washing and air drying; anddropping fluorescnet or enzyme labeled streptavidin diluted by blockage liquid, washing and air drying. The protein chip of the present invention is used in determining antigen and antibody, and detecting specific matter combined with protein. The improved method of the present invention has clear, stable and reliable test results and wide application range.

The invention discloses a local discharge detection equipment and method of a switch cabinet. The detection equipment comprises a ceramic capacitor core insulator, a local discharge signal extractiondevice, a charge indicating device and a local discharge detection device. The charge indicating device comprises a charge indicating circuit. The local discharge signal extraction device is connectedwith the charge indicating device in parallel, the local discharge signal extraction device comprises an energy supply capacitor and a signal extraction resistor which are sequentially connected in series, and a local discharge detection point is arranged between the energy supply capacitor and the signal extraction resistor. A coupling capacitor is arranged in the ceramic capacitor core insulator, and the ceramic capacitor core insulator is connected to a grounding wire of the charge indicating device in series. One end of the local discharge detection device is electrically connected with the local discharge detection point, and the other end of the local discharge detection device is grounded. When local discharge signals are measured, measurement is convenient, detection signals are strong, and the anti-interference capacity is high.

The invention relates to a method for nondestructively testing micro-defects in sheet ferromagnetic materials based on measurement of magnetostatic force and belongs to the technical field of nondestructive testing. The method comprises the following steps: arranging a permanent magnet on the surface of a calibration sheet, and measuring reacting force of magnetostatic force which acts on the permanent magnet by using a force sensor; arranging the permanent magnet on the surface of a sheet to be measured, and measuring reacting force stressed by the permanent magnet by using the force sensor; and comparing the two reacting forces, if the same, determining that a surface defect or an internal defect does not exist on the sheet to be measured, and if different, determining that a surface defect or an internal defect exists on the sheet to be measured. The method is simple in measurement process, relatively low in detection cost and reliable in detection result; the measurement accuracy is high, and micron order micro-defects in the magnetic sheets can be detected; the detection speed is high, and sheet materials can be rapidly detected on line in real time.

The invention discloses an oscillation wave detection transformer winding deformation circuit and a method. The oscillation wave detection transformer winding deformation circuit comprises a direct current high voltage power supply, wherein the direct current high voltage power supply is connected to a three phase neutral point of a primary winding; a short circuit switch is arranged between the direct current high voltage power supply and the primary winding; one terminal of the short circuit switch is electrically connected to a position between the direct current high voltage power supply and the primary winding; the other terminal of the short circuit switch is grounded; the end screen of a bushing capacitor of each phase of the primary winding is electrically connected with a voltage-dividing capacitor; the two terminals of the voltage-dividing capacitor are connected to a waveform measurement and analysis device; all the voltage-dividing capacitors are grounded. The oscillation wave detection transformer winding deformation circuit forms a leakage reactance or excitation impedance oscillation circuit of a ground stray capacitor, the bushing capacitors and the primary windingto a secondary winding through closed grounding of the short circuit switch, measures a three phase voltage response curve of the primary winding through the waveform measurement and analysis device,analyzes and compares the three phase voltage response curve to judge whether a transformer winding is deformed. The oscillation wave detection transformer winding deformation circuit is applicable totransformer winding deformation potential fault detection.

A record carrier has substantially parallel tracks having first variations of a first physical parameter and second variations of a second physical parameter of the track. The first variations represent information recorded on the record carrier, this information being recoverable by a controllable type of data processing. A modulation pattern of the second variations represents a code for controlling the type of data processing. In respect of a code track that includes the modulation pattern, the modulation pattern in the left neighboring track is aligned to the modulation pattern in the right neighboring track. Thereby crosstalk due to the second variations is decreased, and at least two tracks have the same modulation pattern. The playback device has a demodulator (50) and a control unit (56) for retrieving the code from at least two tracks.

The invention discloses a detecting method for immunosuppressive agents in dried blood spots, and further discloses a detecting kit for the immunosuppressive agents in the dried blood spots. The detecting method comprises the steps that the standard curve dried blood spots and the to-be-detected dried blood spots are mixed with an extracting solution and an internal standard solution correspondingly and then extracted, centrifuging is conducted, and supernate is taken; the supernate is blown dry and then redissolved through a redissolving solution, and detecting is conducted through a tandem mass spectrum method; according to the peak exceeding area specific value of standard samples to an internal standard object and the concentration of the standard samples, linear regression is conducted to obtain a standard curve equation of the immunosuppressive agents; the peak exceeding area specific value of to-be-detected samples to the internal standard object is substituted into the standardcurve equation, and the content of the immunosuppressive agents in the to-be-detected samples is calculated; the extracting solution is a water solution of methyl alcohol and/or acetonitrile; and theimmunosuppressive agents are one or more of cyclosporine A, tacrolimus, sirolimus and everolimus. The detecting method can effectively detect the four types of immunosuppressive agents simultaneously, and thus the detecting efficiency is improved.

The invention provides a construction method for an engineering strain of Escherichia coli used for detecting the heavy metal mercury and establishment of a method for detecting mercury in a water body by using the engineering strain of Escherichia coli. The genetic engineering strain is merR:: PmerT:: cfp-linker-cfp/E.coli delta zntA, named as E.coli WMC-010 (CTMCC No. 9749), wherein mer operon originated from Serratia marcescens is knocked into the zntA position of the genome of a wild E. coli MC4100 strain to start expression of cyan fluorescent protein (CFP). The engineering strain provided by the invention can overcome the defects of a high fluorescence background value, poor independent experiment repeatability and difficulty in accurate quantification of the heavy metal mercury in a water body of a reporter strain containing reporter plasmid and can reflect bio-availability of mercury in a water body, so bases are provided for objective evaluation of the biotoxicity of the heavy metal mercury in a water body.

The invention provides a multi-immunohistochemical analysis kit for hodgkin lymphoma, a use method and application thereof, wherein the kit, the use method and the application relate to the field of multi-immunohistochemical technology. According to the multi-immunohistochemical analysis kit, a monoclonal antibody set comprises more than two and at most six components selected from: a CD3 monoclonal antibody, a CD30 monoclonal antibody, a CD68 monoclonal antibody, a CD56 monoclonal antibody, an LAG3 monoclonal antibody, a PD1 monoclonal antibody and a PDL1 monoclonal antibody. The multi-immunohistochemical analysis kit according to the invention detects a human or animal in-vitro tissue to which a immunization inspection point inhibitor is applied as a sample, and furthermore is used for determining effectiveness of the immunization inspection point inhibitor to the hodgkin lymphoma. The invention further provides a use method of the multi-immunohistochemical analysis kit, wherein no more than six immunization inspection points can be effectively marked on the same tissue, and no cross reaction exists among the multiple marks.

A pulse fusion mass spectrometry element analysis method belongs to the technical field of quantitative analysis of chemical components of materials. The steps are: pulse melting and degassing: under the protection of inert gas, the pulse furnace is used to heat and melt the sample, and the oxygen, nitrogen, hydrogen and argon gas elements in inorganic materials such as metals and ceramics are converted into gaseous components in a graphite crucible. Carbon monoxide and carbon dioxide, nitrogen, hydrogen and argon are released; purification treatment: the degassed product is carried out by the carrier gas and purified; mass spectrometry detection: the purified gas is introduced into the mass spectrometer, and the degassed product is ionized and focused into an ion beam , is accelerated by an electric field, separated according to the mass-to-charge ratio, and finally reaches the mass spectrometer to generate a series of electrical signals; after signal collection and data processing, the mass percentages of oxygen, nitrogen, hydrogen and argon elements in the tested sample are obtained. The advantage is that it can realize the simultaneous, fast, high sensitivity and high precision analysis of the above four elements. Sensitivity of 0.1ppm to 0.01ppm.

The invention discloses an aviation metal small diameter tube defect internal inspection probe and a method. The probe includes a disc coil and a circumferential rectangular coil group array that arefixed on a photosensitive resin coil skeleton together. The disc coil and the photosensitive resin coil skeleton are coaxial, rectangular coils in the circumferential rectangular coil group array areevenly arranged along the circumference, adjacent rectangular coils are in contact with each other, and the disc coil passes perpendicularly through the air field surrounded by each rectangular coil;the winding groups of the disc coil and the rectangular coils are respectively obtained by winding of three-core-wire groups, wherein each three-core-wire group comprises three side-by-side same-diameter copper core enameled wires; the winding group of each coil comprises three independent coils, a middle coil is an excitation coil, two side coils are detection coils, the two detection coils are connected in differential arrangement, and difference of induced voltage signals picked up by the two detection coils is taken as an output signal. The present invention also provides the detection method of the probe, and the detection method can detect aviation metal small diameter tube defects quickly and highly accurately, and has important engineering application value.

The invention discloses a rapid biology sterilization detection tube device, which comprises a liquid containing tube for containing a culture solution and a culture tube for containing a thallus carrier, wherein the culture tube is divided into two tube bodies of an upper part and a lower part; the upper part tube body of the culture tube is arranged at the outer surface of the liquid containing tube in a sleeving manner; the lower part tube body is used for containing the thallus carrier; the diameter of the lower part tube body, for containing the thallus carrier, of the culture tube is smaller than that of the upper part tube body; the upper and lower ends of the liquid containing tube are provided with openings; the lower end opening of the liquid containing tube is pasted with a heat-seal bonding sheet; the corresponding culture tube is internally provided with a bursting device; the bursting device is used for piercing the bonding sheet at the lower end of the liquid containing tube when the liquid containing tube is put into the culture tube; 2 to 7 days originally required to observe a result are shortened to 1 to 3 hours to obtain a sterilization result.

Provided are particles where a hydrophilic coloring agent having an anionic functional group, such as ICG, hardly leaks from the particles. The particles include a coloring agent having an anionic functional group, such as ICG, ions of a polyvalent metal, such as iron ions (Fe²⁺ or Fe³⁺), zinc ions, and a polyol compound.

A detector for electromagnetic radiation in the range 80 GHz to 4 THz comprises a laser light source (115) an optical modulator (13) arranged to modulate light from the laser light source (11) and a filter system (17) for selecting a defined range of frequencies of the modulated light. The optical modulator is an electroabsorption modulator (13) with an antenna (15) which is sensitive to electromagnetic radiation in the range 80 GHz to 4 THz. The signal received by the antenna (15) modulates the electric field across the electroabsorption modulator (13), whereby to modulate the light from the laser light source (11).

The invention provides a multiple immunohistochemical analysis kit for liver cancer, and a using method and application thereof, and relates to the technical field of multiple immunohistochemistry. The multiple immunohistochemical analysis kit limits that monoclonal antibody groups comprise a first monoclonal antibody group and a second monoclonal antibody group; the first monoclonal antibody group comprises a PanCK monoclonal antibody, a PDL1 monoclonal antibody, a CD8 monoclonal antibody, a CD66B monoclonal antibody and a CD163 monoclonal antibody; the second monoclonal antibody group comprises a PanCK monoclonal antibody, a PDL1 monoclonal antibody, a CD8 monoclonal antibody, a PD1 monoclonal antibody and a CD68 monoclonal antibody. According to the multiple immunohistochemical analysiskit provided by the invention, the paraffin sections of the human liver tissue in vitro are used as samples for detecting, and thus the effectiveness of the immune checkpoint inhibitor acting on theliver cancer is predicted.

The invention relates to a three-channel sprayer nozzle of a novel structure. The sprayer nozzle comprises a quartz tube, an air channel, a tee joint, an electrode wire elastic head, an air inlet pipe locking screw head, an air inlet tube, an electrode wire locking screw head, a high-pressure locking screw head, a high-voltage wire, a high-voltage electrode, a liquid inlet tube, a liquid inlet tube locking screw head, a quartz tube elastic head, a quartz tube locking screw head and an electrode wire. Air inlet component, liquid inlet components and high voltage applying components are all arranged on the tail portion of the tee joint, three hollow screw heads, that is the air inlet tube locking screw head, the liquid inlet tube locking screw head and the electrode wire locking screw head, are distributed on the tee joint in a mutually angled mode, and the air inlet tube, the liquid inlet tube and the electrode are introduced into the tee joint through center holes of the three screw heads. The sprayer nozzle is compact in structure and small in size, is mainly used for desorption atmospheric pressure chemical ionization and is especially suitable for small direct mass spectrometry.

The sensor includes the detection units fixed on the two sides of the flat plate symmetrically. Each detection unit includes the body case possessing the through holes parallel to the axis m-m of theflat plate. The channel connects the through hole and the axis out hole. The elastic membranes seal the two ends of the through hole. There are two piezoelectric ceramic sheets in the through hole. One end of each ceramic sheet is fixed on the elastic membrane and the other ends of fixed on the metal block between the ceramic sheets. There is the gap between the periphery of the ceramic sheets, the metal block and the wall of the through hole. The lead wire of the metal block via the channel and through the axial out hole of the plate is leaded out.

The invention provides a kit and a usage method thereof. The kit comprises one or more pairs of 16 upstream and downstream primer pairs for amplifying STR loci, namely, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, vWA, Penta E, Penta D and Amelogenin, wherein the sequences of the 16 pairs of upstream and downstream primers are shown as SEQ ID NO:1 to SEQ ID NO:32. By adopting the kit and the usage method provided by the invention, the related 16 pairs of upstream and downstream primers have the advantages of high specificity and high amplification efficiency, and genotyping of the 16 STR loci can be performed rapidly and accurately.

The invention discloses a multi-parameter radar level transmitter. The transmitter comprises a first radar level meter, a second radar level meter, a sleeve and a flange plate, the first radar level meter and the second radar level meter are both connected to the flange plate, and the detection ends of the first radar level meter and the second radar level meter both penetrate through the flange plate. The sleeve sleeves the outside of the detection end of the first radar level meter, one end of the sleeve faces the end portion of the flange plate and is fixedly connected with the flange plate, the sleeve is made of conductive materials and used for preventing electromagnetic waves emitted by the detection end of the first radar level meter and the detection end of the second radar level meter from interfering with each other. The multi-parameter radar level transmitter provided by the invention can measure the liquid level and the interface at the same time, and is convenient to control and compare data.

The invention discloses a suspension type self-vibration suppression differential flow sensor which comprises two detection units symmetrically fixed at two sides of a panel, each detection unit comprises a shell, the shell has a through hole, which is parallel to the axis m-m of the panel, the through hole is connected with an axial direction outlet hole of the panel through a channel, the connection end of the through hole is sealed via the elastic membrane, two piezoelectric ceramic pieces are formed in the through hole, one of each of two piezoelectric ceramic pieces is fixed with the elastic membrane while the other end is fixed with a metal block between the piezoelectric ceramic pieces, the gaps are respectively formed between two piezoelectric ceramic pieces and the periphery of the metal block and the through hole, the lead of the metal block is guided outward through the outlet hole of the channel. The sensor has higher measuring sensitivity; stronger detection signal can be achieved at small flow, the suspension type interior structure has stronger anti-vibration property, greater measuring range and high reliability.

The invention provides an enzyme-free uric acid sensor based on Prussian blue with high detection precision, high speed and stable performance. The enzyme-free uric acid sensor comprises a substrate,a conducting wire layer and an electrode layer, which are sequentially stacked together, the electrode layer comprises a counter electrode and a working electrode, the working electrode is a Prussianblue carbon paste coating on the substrate, and Prussian blue carbon paste is formed by mixing Prussian blue powder with carbon paste. The counter electrode and the working electrode have respective electrode terminals, and the counter electrode and the working electrode are respectively connected with the respective electrode terminals through first conducting wires, the electrode terminals and the first conducting wires are all located on the conducting wire layer, and the first conducting wire comprises a silver chloride coating and a Prussian blue carbon paste coating covering the silver chloride coating.