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Enzyme-free uric acid sensor based on Prussian blue

A Prussian blue and sensor technology, applied in the field of electrochemistry, can solve the problems of increased production cost, complicated preparation steps, failure of electrode sheets, etc., and achieves the effects of cost saving, simple preparation process and stable performance

Pending Publication Date: 2019-08-20
SHANDONG IND TECH RES INST OF ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this patent are: 1. It is necessary to form a working electrode on the substrate first, and then coat the Prussian blue / N-doped carbon nanocomposite material on the working electrode, and the process is complicated; and the Prussian blue / N-doped carbon nanocomposite The material is easy to fall off from the surface of the working electrode, resulting in the failure of the electrode sheet
2. The preparation steps of Prussian blue / N-doped carbon nanocomposites are complicated, which increases the production cost

Method used

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  • Enzyme-free uric acid sensor based on Prussian blue
  • Enzyme-free uric acid sensor based on Prussian blue
  • Enzyme-free uric acid sensor based on Prussian blue

Examples

Experimental program
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Effect test

Embodiment 1

[0061] like figure 1 and figure 2 As shown, an enzyme-free uric acid sensor based on Prussian blue includes a substrate 1 , a wire layer 2 , an electrode layer 3 , an insulating layer 4 , a double-sided adhesive layer 5 and a hydrophilic film layer 6 sequentially stacked. The electrode layer includes a counter electrode 31 and a working electrode 32. The working electrode 32 is a coating of Prussian blue carbon paste on the substrate, and the Prussian blue carbon paste is formed by mixing Prussian blue (PB) powder and carbon paste. The mass fraction of Prussian blue in the Prussian blue carbon paste is 0.5%. Prussian blue is used to detect uric acid. The counter electrode 31 is a silver chloride coating on the substrate. The mass fraction of Prussian blue refers to the percentage of Prussian blue mass in the total mass of Prussian blue carbon paste. The counter electrode and the working electrode are parallel, and the counter electrode and the working electrode are perpen...

Embodiment 2

[0079] The enzyme-free uric acid sensor based on Prussian blue prepared in this example has the same structure as the enzyme-free uric acid sensor in Example 1, except that the mass fraction of Prussian blue in the Prussian blue carbon slurry is 3.5%.

[0080] In this embodiment, the preparation method of the enzyme-free uric acid sensor based on Prussian blue comprises the following steps in turn:

[0081] 1) Ultrasonic cleaning of the PET substrate in pure water for 15 minutes, and drying in an oven at 90°C for 30 minutes.

[0082] 2) Use silver chloride paste to print the wire layer and the counter electrode on the surface of the substrate, put them in an oven at 120°C and dry for 15 minutes; the wire layer includes electrode terminals, two identification terminals, the first wire and the second wire.

[0083] 3) Prussian blue powder is added in the carbon slurry to mix and prepare Prussian blue carbon slurry, the mass fraction of Prussian blue in the Prussian blue carbon s...

Embodiment 3

[0090] The enzyme-free uric acid sensor based on Prussian blue prepared in this example has the same structure as the enzyme-free uric acid sensor in Example 1, except that the mass fraction of Prussian blue in the Prussian blue carbon slurry is 5%.

[0091] In this embodiment, the preparation method of the enzyme-free uric acid sensor based on Prussian blue comprises the following steps in sequence:

[0092] 1) Ultrasonic cleaning of the PET substrate in pure water for 15 minutes, and drying in an oven at 90°C for 30 minutes.

[0093] 2) Use silver chloride paste to print the wire layer and the counter electrode on the surface of the substrate, put them in an oven at 120°C and dry for 15 minutes; the wire layer includes electrode terminals, two identification terminals, the first wire and the second wire.

[0094] 3) Prussian blue powder is added in the carbon slurry and mixed to prepare Prussian blue carbon slurry, the mass fraction of Prussian blue in the Prussian blue carb...

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Abstract

The invention provides an enzyme-free uric acid sensor based on Prussian blue with high detection precision, high speed and stable performance. The enzyme-free uric acid sensor comprises a substrate,a conducting wire layer and an electrode layer, which are sequentially stacked together, the electrode layer comprises a counter electrode and a working electrode, the working electrode is a Prussianblue carbon paste coating on the substrate, and Prussian blue carbon paste is formed by mixing Prussian blue powder with carbon paste. The counter electrode and the working electrode have respective electrode terminals, and the counter electrode and the working electrode are respectively connected with the respective electrode terminals through first conducting wires, the electrode terminals and the first conducting wires are all located on the conducting wire layer, and the first conducting wire comprises a silver chloride coating and a Prussian blue carbon paste coating covering the silver chloride coating.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to an enzyme-free uric acid sensor based on Prussian blue. Background technique [0002] The following background art is provided to help the reader understand the present invention and is not to be admitted as prior art. [0003] At present, the detection principle of the electrode is mostly to modify the enzyme corresponding to the substrate on the working electrode, and measure the electrical signal through the enzymatic reaction, so the uric acid sensor generally uses the uricase method. However, the conditions for enzyme immobilization on the working electrode are relatively harsh, and the activity of the enzyme needs to be kept unaffected during the immobilization process, and both temperature and humidity need to be considered during enzyme immobilization. In addition, the price of uricase is generally relatively expensive, which limits its scope of use and increases the prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/30G01N27/48
Inventor 叶学松梁波赵欢萍
Owner SHANDONG IND TECH RES INST OF ZHEJIANG UNIV
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