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A kind of enzyme-free colorimetric detection method of uric acid

A detection method, uric acid technology, applied in the field of analytical chemistry, can solve the problems of high requirements for reagent storage conditions, harsh environmental requirements, and complicated operations, and achieve the effects of simple operation, sensitive response, and accurate measurement results

Active Publication Date: 2019-12-31
江阴智产汇知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned disclosed detection methods have common shortcomings in that one or more natural enzymes are required to participate, and the environmental requirements for the detection process are harsh; the cost is relatively high, the operation is relatively complicated, and the reagent storage conditions are high.

Method used

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  • A kind of enzyme-free colorimetric detection method of uric acid
  • A kind of enzyme-free colorimetric detection method of uric acid
  • A kind of enzyme-free colorimetric detection method of uric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The integrated CoP nanosheet array as a method for preparing a monolithic peroxidase-like enzyme includes the following steps:

[0041] 1) Cut the commercial nickel foam into a 1cm×1cm square, soak it in 37% hydrochloric acid for 30 minutes and then ultrasonically clean it with deionized water for 10 minutes, and then move it to a 60°C constant temperature drying oven to dry to obtain pure foam nickel;

[0042] 2) Dissolve 1.4055g of cobalt sulfate and 0.3g of urea into 30mL of deionized water, and mix well by stirring at room temperature for 10 minutes;

[0043] 3) Put the processed nickel foam (30 pieces) and the above solution in a hydrothermal reaction kettle at 80℃ for 12h to obtain Co(OH) 2 Nickel foam composite modified by nanosheet array;

[0044] 4) After rinsing the above compound with deionized water, place it in an oven at 60°C for drying. The dried Co(OH) 2 / NF tablets (6 tablets) and 300mg sodium hypophosphite are put into two adjacent porcelain boats (the porcela...

Embodiment 2

[0049] The integrated CoP nanosheet array as a method for preparing a monolithic peroxidase-like enzyme includes the following steps:

[0050] 1) Cut the commercial nickel foam into a 1cm×1cm square, soak it in 37% hydrochloric acid for 30 minutes and then ultrasonically clean it with deionized water for 10 minutes, and then move it to a 60°C constant temperature drying oven to dry to obtain pure foam nickel;

[0051] 2) Dissolve 1.4055 g of cobalt sulfate and 0.9 g of urea into 60 mL of deionized water, and mix them evenly by stirring at room temperature for 20 minutes;

[0052] 3) Put the processed nickel foam (30 pieces) and the above solution in a hydrothermal reaction kettle at 150°C for 24 hours to obtain Co(OH) 2 Nickel foam composite modified by nanosheet array;

[0053] 4) After rinsing the above compound with deionized water, place it in an oven at 60°C for drying. The dried Co(OH) 2 / NF tablets (6 tablets) and 450mg sodium hypophosphite were put into two adjacent porcelain ...

Embodiment 3

[0058] The integrated CoP nanosheet array as a method for preparing a monolithic peroxidase-like enzyme includes the following steps:

[0059] 1) Cut the commercial nickel foam into a 1cm×1cm square, soak it in 37% hydrochloric acid for 30 minutes and then ultrasonically clean it with deionized water for 10 minutes, and then move it to a 60°C constant temperature drying oven to dry to obtain pure foam nickel;

[0060] 2) Dissolve 1.4055 g of cobalt sulfate and 1.9 g of urea into 100 mL of deionized water, and mix them evenly by stirring at room temperature for 20 minutes;

[0061] 3) Put the processed nickel foam (30 pieces) and the above solution in a hydrothermal reaction kettle at 120°C for 48 hours to obtain Co(OH) 2 Nickel foam composite modified by nanosheet array;

[0062] 4) After rinsing the above compound with deionized water, place it in an oven at 60°C for drying. The dried Co(OH) 2 / NF tablets (6 tablets) and 600mg sodium hypophosphite are put into two adjacent porcelain ...

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Abstract

The invention belongs to the technical field of analytical chemistry and relates to a method for non-enzymatic colorimetric detection of uric acid. First an integrated CoP / NF entire mimetic peroxidase is prepared, and then a color-development substance TMBox is generated by redox reaction of catalyzing TMB and a hydrogen peroxide solution with CoP / NF serving as mimetic peroxidase. Since TMBox can be selectively reduced into colorless TMB by uric acid, the absorbance generated by the original chromogenic reaction reduces with increase of the uric acid concentration, a curve with the absorbance reducing with the increase of the uric acid concentration is obtained by measuring the absorbance of the finally obtained color-development substance, and the uric acid content of a measured object is determined according to the standard curve. According to the analytical method, neither peroxidase nor uric acid oxidase is needed to achieve non-enzymatic detection of uric acid, the detection cost is low, and the operation is simple; the detection range of the uric acid is 1 to 200 [mu]m, and the detection limit is as low as 1 [mu]m; the response to the uric acid is sensitive; the method has good specificity, and accurate analysis of uric acid in serum and urine can be achieved.

Description

Technical field [0001] The invention belongs to the technical field of analytical chemistry, and relates to a detection method of uric acid, in particular to an enzyme-free colorimetric detection method of uric acid. Background technique [0002] The normal concentration of uric acid in adult urine is 149-446mmol / L and that in serum is 0.15-0.4mmol / L. Uric acid is one of the final products of purine metabolism in the human body, and most of it is excreted in urine. When the concentration of uric acid in the body is too high or too low, it is easy to cause many diseases, such as gouty arthritis, secondary hypertension, cerebral infarction, ureteral stones and kidney disease. In severe cases, it may cause kidney failure and endanger life. Therefore, accurate detection of uric acid in human blood and urine has become an important method for the diagnosis and prevention of related diseases. [0003] In clinical practice, the detection of uric acid is usually achieved through the combi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 何艳芳牛湘衡张文驰齐飞宋洪伟潘建明张锡凤
Owner 江阴智产汇知识产权运营有限公司
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