205 results about "Enzyme free" patented technology

The invention provides a nitrogen-doped porous vertical graphene nanowall array. A compact coating layer is formed by means of nickel hydroxide serving as a template and the self-assembly property of dopamine, then high-temperature carbonization is conducted to prepare a nitrogen-doped vertical graphene nanowall array material, in-situ functional modification is conducted on the basis, the compound functional material loaded with noble metal nanoparticles, noble metal alloy nanoparticles, metal oxides, metal sulfide, metal phosphide, conductive polymers and the like is obtained, and application in the fields of supercapacitors, lithium ion batteries, water decomposition, electrochemical catalysis, enzyme-free biosensors and the like is explored.

The invention provides a preparation method of a carbon-based compound slow-release fertilizer. Raw materials comprise, by weight, 5 to 10 parts of straw biological black carbon, 2 to 5 parts of pyroligneous liquor, 21 to 34 parts of enzyme-free bacteria chicken manure, 10 to 15 parts of bone meal, 18 parts of potassium sulfate, 22 to 24 parts of ammonium biphosphate, 11 to 12 parts of large-granule urea and 2 to 5 parts of water. The preparation method comprises putting the crushed enzyme-free bacteria chicken manure, straw biological black carbon, bone meal, large-granule urea, ammonium biphosphate and potassium sulfate into a ZLC-300 type mixer for mixing and stirring, putting them into a KP-300 type organic fertilizer granulator for granulating after uniformly mixing, adding 2 to 5 parts of the pyroligneous liquor and 2 to 5 parts of water while granulating, naturally cooling the resulting to be lower than 28 DEG C after the granulation, and packaging it, wherein the enzyme-free bacteria chicken manure is disinfected for 2 to 3 hours under 2 Pa in a disinfection pot, dried in a tumble dryer, crushed, and sieved by a 60-mesh sieve, with a water content being less than 11 %.

Novel compounds having a fluorescence quencher as a leaving group are disclosed. Nucleic acids and other molecules containing a fluorophore and a fluorescence quencher are disclosed as an embodiment of this invention. The use of the oligonucleotides in enzyme-free oligonucleotide ligation reactions results in an increase in fluorescence when the oligonucleotide also contains a nearby fluorophore. The ligation reactions can be performed in solution, on surfaces, or in cells.

The invention provides a signal amplification and signal reporting integrated oligonucleotide probe. The probe is a pair of DNA hairpin sequences designed on the basis of a target molecule and having cohesive ends, substantially is a product combining a hybridization chain reaction fuel molecule with a G-quadruplex sequence, has the characteristic of target molecule signal cascade amplification of the hybridization chain reaction in an enzyme-free isothermal manner, and enables the amplified target molecule signal to be conveniently and simply detected through the introduction of a signal reporting element G-quadruplex sequence. The invention also provides a method of an enzyme-free isothermal nucleic acid probe mediated by the above probe. The method enables the signal of an object to be detected to be amplified and detected under a normal temperature condition without any enzymes, and has the advantages of sensitivity, accuracy, simplicity, and easy implementation.

The invention discloses a construction method of a cuprous oxide membrane-based enzyme free-oxygen sensitive glucose photo electrochemical sensor. The construction method comprises the following steps: with an ITO conductive glass as a working electrode, a platinum wire as a counter electrode and a silver/ silver chloride electrode as a reference electrode, inserting the three electrodes into a sodium hydroxide solution containing 0.05mol/L copper sulfate and 0.1mol/L sodium citrate, and regulating a pH value to be 11; under the conditions that the deposition temperature is 60 DEG C, the deposition potential is -0.4V and the deposition time is 20 minutes, forming a cuprous oxide membrane on the surface of the ITO conductive electrode; and rinsing residual solution on the surface with pure water, drying for 1h at the temperature of 100 DEG C, and forming a stable cuprous oxide membrane-based photocathode. According to the construction method disclosed by the invention, photocurrent detection based on light excitation is low in detection signal background and can have high detection sensitivity and stability without needing expensive instrument and equipment and complex sample treatment.

The invention discloses a nickel-cobalt alloy/polypyrrole/reduced graphene nanometer composite material and an application thereof. A method for preparing the composite material includes the steps that 1, a pyrrole monomer, graphite oxide and a reducing agent are prepared into a polypyrrole/reduced graphene nanometer composite with the in-situ polymerization method; 2, the polypyrrole/reduced graphene nanometer composite prepared in the step 1 is taken and dispersed into ethylene glycol, then nickel salt and cobalt salt are added, and the mixture is heated, reacted, washed and dried to obtain the nickel-cobalt alloy/polypyrrole/reduced graphene nanometer composite material. As polypyrrole and reduced graphene are combined, the agglomeration defect of graphene is overcome, electrical conductivity is improved, and a unique three-dimensional surface structure is formed. The composite material serves as a substrate material, more nickel-cobalt alloy particles can be more evenly adsorbed, an excellent electrochemical catalysis response is shown in the application to a glucose sensor accordingly, and a novel enzyme-free glucose sensor is configured.

The invention relates to a preparation method of a Pt-Ni alloy nano tube array electrode and an application for an enzyme-free glucose sensor. A Ni nano wire array acts as a template, and the Pt-Ni alloy nano tube array electrode is prepared by adopting a current displacement method. The preparation method comprises the following steps: carrying out metal spraying treatment on one surface of a porous template comprising an AAO template and a PC template, fixing conductive silver glue on the surface of the electrode, and sealing the circumference of the template with insulation rubber; carrying out electric deposition in the porous template so as to prepare a Ni nano wire, and then removing the template so as to obtain the Ni nano wire array electrode; and preparing the Pt-Ni alloy nano tube array electrode by adopting a current displacement method, wherein a precursor solution is chloroplatinic acid or potassium chloroplatinate. The Pt-Ni alloy nano tube array electrode can be applied to an enzyme-free glucose sensor. The linear response range of the enzyme-free glucose sensor to glucose is 1-9Mm, and the detection sensitivity is 57.87micron AmM-1.

The present invention relates to sequencing probes, methods, kits, and apparatuses that provide enzyme-free, amplification-free, and library-free nucleic acid sequencing that has long-read-lengths and with low error rate.

The invention relates to a chip enzyme-free glucose sensor electrode based on three-dimensional copper oxide nanometer flowers as well as a preparation method and application thereof. In the invention, the nanometer flowers directly grow in situ on the surface of a copper foil; the nanometer flower-based chip enzyme-free glucose sensor electrode is composed of a copper foil substrate and a three-dimensional copper oxide nanometer flower array; and the three-dimensional copper oxide nanometer flowers with diameters of 10-20 microns exist in a crystal morphology and are uniformly and compactly distributed on the surface of the electrode. The preparation method comprises the steps of selecting a copper foil slice with a thickness of 0.2-0.5mm, putting the cleaned and dried copper foil into a KOH solution at a temperature about 30-40 DEG C for 3-9 days, after the reaction is completed, taking out a sample, repeatedly washing by utilizing distilled water and deionized water and drying at a room temperature. The three-dimensional copper oxide nanometer flower-based chip enzyme-free glucose sensor electrode can be applied to electronic equipment for continuously monitoring blood glucose concentration.

The invention relates to a preparation method of a Pt nanometer particle-modified Cu nanowire array electrode and a use of the Pt nanometer particle-modified Cu nanowire array electrode in an enzyme-free glucose sensor. The preparation method comprises spraying gold to a porous AAO template, fixing Cu foil to the surface of the electrode with a sprayed gold layer through a conductive adhesive, sealing the surrounding parts of a template through insulative silicone rubber, constructing a three-electrode system of the template as a work electrode, a saturated calomel electrode as a reference electrode and a platinum electrode as a counter electrode, putting the three-electrode system into CuSO4 and H2SO4 electrolyte, carrying out constant current electro-deposition, then carrying out immersion in a NaOH solution to remove the AAO template so that a Cu nanowire array structure is obtained, carrying out repeated cleaning through distilled water and carrying out constant potential deposition in H2PtCl6 and H2SO4 electrolyte to obtain the Pt nanometer particle-modified Cu nanowire array electrode. The Pt nanometer particle-modified Cu nanowire array electrode is used for an enzyme-free glucose sensor.

The invention discloses a Pt nano flower micro needle type enzyme-free glucose sensor electrode and a preparation method thereof. The electrode is characterized by being composed of a pt nano flower, wherein average length of single pedal is 100nm-200nm, and average width of single pedal is 50nm-100nm. The Pt nano flower micro needle type enzyme-free glucose sensor electrode is prepared by using an ultrasonic intermittent constant potential electrolytic deposition method, the preparation method is simple in process, the reaction conditions are easy to control, and the binding force between the Pt nano flower and the needle electrode is strong. The preparation method has the advantage that the enzyme-free glucose sensor electrode can be formed on the micro needle electrode surface, wherein the electrode has better electrocatalytic oxidation performance to the glucose, and the formed micro needle type enzyme-free glucose sensor has a wide linearity range, high response sensitivity, low detection limit and high stability for the glucose, and has better application foreground in the aspect of implanted glucose sensors.

The invention belongs to the technical field of biology and particularly relates to a detecting kit for a specific IgE antibody (quantitative) of a food allergen as well as preparation and detecting methods of the detecting kit. The detecting kit is used for quantitatively detecting the content of the specific IgE antibody (quantitative) of the food allergen in human serum in vitro. The detecting kit for the specific IgE antibody of the food allergen contains the following components: a magnetic separation reagent, an allergen reagent, an enzyme reactant, a calibrated product, a quality control product, a concentrated cleaning solution and a substrate solution, wherein the magnetic separation reagent contains magnetic particles on which human IgE antibodies are coated, and the allergen reagent contains a biotin-labeled specific allergen. The detecting method of the detecting kit for the specific IgE antibody of the food allergen is realized by combining a chemiluminescence immune assay technology and a magnetic particle separation technology, and meanwhile, an enzyme-free capturing method is adopted, so that the detecting method has the advantages of convenience in operation, high sensitivity, good accuracy, high speed and no pollution.

A method for detecting aflatoxin B1 based on hybridization chain reaction and DNAzyme signal amplification fluorescence detection, the invention relates to the construction and application of a signal amplification technology fluorescence spectrum sensor. Using the target aflatoxin B1 to trigger a change in the conformation of the aptamer DNA, promote the assembly of the hairpin probe to form a long-chain repetitive DNAzyme unit, and catalyze the enzyme-free AFB1 sensing strategy that cuts the molecular light to generate a fluorescent signal. Relying on this method, the detection limit can reach 0.5 ng/mL, which is far lower than the national standard (10 ng/mL). In addition, we tested aflatoxin B1 in the moldy rice extract and also obtained satisfactory results.

The invention discloses an enzyme-free glucose electrochemical sensor and a detection method thereof. The enzyme-free glucose electrochemical sensor comprises a three-electrode system consisting of areference electrode, a counter electrode and a working electrode, wherein the working electrode is made of a material with electrocatalytic oxidation performance for glucose; the method for detectingglucose by using the enzyme-free glucose electrochemical sensor comprises the following steps: (1) electrochemical pretreatment of the working electrode: applying high negative potential; (2) electrochemical oxidation of glucose: applying the potential required for glucose oxidation; (3) electrochemical cleaning of the working electrode: applying positive potential. The influence of pH of a sampleon a detection result can be removed, enzyme-free glucose detection originally required under the alkaline condition can be performed in neutral and acidic samples, and the sensor has the advantagesof good electrode stability, high sensitivity, good repeatability and the like, and has a very broad application prospect.

The invention discloses a preparation method and applications of an urchin-shaped Ni-Zn metal organic skeleton hollow sphere nano material, and belongs to the technical field of enzyme-free glucose sensor preparation. According to the preparation method, an ethanediol mixed solution of a bivalent nickel salt and a bivalent zinc salt is mixed with a DMF solution of terephthalic acid, hydrothermal reaction is carried out at 150 to 200 DEG C, and an obtained reaction precipitate is collected; the reaction precipitate is washed with DMF and ethanol, and is dried so as to obtain a finished product. The urchin-shaped Ni-Zn metal organic skeleton hollow sphere nano material is obtained via simple hydrothermal reaction; the raw materials are nontoxic, are friendly to the environment, and are cheap; the preparation method is simple, is convenient to control, and is suitable for continuous large-scale production; under ultrasonic conditions, the urchin-shaped Ni-Zn metal organic skeleton hollow sphere nano material is dissolved in a perfluorosulfonic acid type polymer solution, and then a glassy carbon electrode is modified with an obtained mixed solution so as to obtain an enzyme-free glucose sensor rapid in response, and high in sensitivity and stability; and application prospect in the field of blood sugar detection is promising.

A method that comprises providing a polymerized sol-gel material (PSG) and linking an enzyme to a surface of the PSG via covalent linkage is provided. The surface of the PSG is derivatized with a linker that comprises a functional group for linking itself to the surface of the PSG and a functional group for linking itself with then enzyme. The linked-enzyme PSG, or microreactor, is an effective means of at least partially digesting a substrate, such as a biological substrate. The activity of the enzyme of the microreactor may be significantly enhanced, up to 200-fold for example, relative to the activity of the enzyme free of the microreactor. The microreactor is thus an effective vehicle for digesting a substrate such as a biomolecule, a protein, an oligonucleotide, a peptide, a steroid, and/or an organic acid, after which, any remaining substrate and one or more digestion product(s) may be separated and detected. Microreactors and integrated devices that incorporate microreactors, such as columns, pipet tips, wells, and well-plates, are also provided.

Belonging to the technical field of biochemical analytical test, the invention specifically relates to an enzyme-free potentiometric glucose sensor with high selectivity and a detection method thereof. A boric acid derivative containing two boric acid groups is used as a glucose recognition molecule, and a quaternary ammonium salt doped polymer is taken as the electrode. The sensor provided by the invention is employed to detect the concentration of glucose in a whole blood sample, and the obtained result is consistent with that of commercially available glucometers.

The invention relates to a reusable enzyme-free glucose detection device and method and belongs to the field of electrochemical biological sensors. A sensor provided by the invention adopts a three-electrode system, wherein a titanium dioxide nanotube array film which modifies platinum nanoparticles serves as a working electrode, a platinum sheet serves as a counter electrode and an Ag/AgCl or 3 mol/L of KCl serves as a reference electrode; the three-electrode system works in an electrolytic tank, an H2SO2 solution with the concentration of 1 mol/L serving as electrolyte is added into the electrolytic tank, and the Pt electrode, the working electrode and the reference electrode are connected with an electrochemical analysis instrument respectively, so that detection can be performed; after detection is finished, a detection function can recover after irradiation is performed for 30 minutes under ultraviolet light; the lower limit of glucose detection is 4*10<-5> mmol/L. Compared with a traditional enzyme method, the enzyme-free detection method has the advantages that the detection limit is increased by above 90%. A novel, simple, convenient, quick, low-cost, stable and reusable enzyme-free glucose detection method is provided.

The invention relates to a Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode, and preparation and application thereof. The preparation method of the electrode comprises the following steps: sanding and polishing an Au electrode on a hide, repeatedly washing with redistilled water, carrying out ultrasonic treatment for 5-10 minutes in an ultrasonic cleaner of which the power is 100-150W, putting in a phosphate buffer solution of which the pH value is 7, and activating for 10-15 cycles by cyclic volt-ampere scanning; dissolving 40-60mg of chitosan in 80-120ml of 0.05 mol/L acetic acid solution to obtain a 0.4-0.6% chitosan solution; and dissolving the prepared Pt-Cu alloy nanoparticles in 0.5ml of 0.4-0.6% chitosan solution, carrying out ultrasonic dispersion, dropwisely coating 40-60 mu l on the Au electrode, and drying at room temperature. The Pt-Cu alloy nanoparticle electrode has the advantages of favorable catalytic activity, favorable electrocatalytic activity for glucose oxidization, wide linear response range, high sensitivity and high selectivity.