Construction method and detection method of cuprous oxide membrane-based enzyme free-oxygen sensitive glucose photo electrochemical sensor

A cuprous oxide and photoelectrochemical technology, applied in the field of photoelectrochemical sensor construction, can solve the problems of glucose oxidase instability, expensive gold/platinum nanoparticles, lengthy modification of electrode steps, etc., and achieve low detection signal background and high detection Sensitivity and stability, effect of low detection limit

Inactive Publication Date: 2015-04-29
YANCHENG INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to solve the shortcoming of the instability of glucose oxidase itself, Dong's research group developed a nano-gold/platinum-titanium dioxide-based non-en

Method used

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  • Construction method and detection method of cuprous oxide membrane-based enzyme free-oxygen sensitive glucose photo electrochemical sensor
  • Construction method and detection method of cuprous oxide membrane-based enzyme free-oxygen sensitive glucose photo electrochemical sensor
  • Construction method and detection method of cuprous oxide membrane-based enzyme free-oxygen sensitive glucose photo electrochemical sensor

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Experimental program
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Embodiment 1

[0036] Embodiment 1: A method for constructing a cuprous oxide film-based non-enzyme-oxygen sensitive glucose photoelectrochemical sensor, comprising the following steps:

[0037] (1) Cut the ITO conductive glass into a size of 1 cm × 4 cm, and contain 1mol L -1 Sodium hydroxide and 0.6% hydrogen peroxide cleaning solution for cleaning, after cleaning, use acetone and ultrapure water to rinse and dry at room temperature;

[0038] (2) The ITO conductive glass treated in step (1) is used as the working electrode, the platinum wire is used as the counter electrode, and the silver / silver chloride electrode is used as the reference electrode, and the three electrodes are inserted into the electrode containing 0.05mol L -1 copper sulfate and 0.1mol L -1 In the sodium hydroxide solution of sodium citrate, adjust the concentration of sodium hydroxide in the solution so that the pH value of the solution is 11;

[0039] (3) Under the condition that the deposition temperature is 50°C t...

Embodiment 2

[0052] Embodiment 2: a method for constructing a cuprous oxide film-based non-enzyme-oxygen sensitive glucose photoelectrochemical sensor, comprising the following steps:

[0053] (1) Cut the ITO conductive glass into a size of 1 cm × 4 cm, and contain 1mol L -1 Sodium hydroxide and 0.6% hydrogen peroxide cleaning solution for cleaning, after cleaning, use acetone and ultrapure water to rinse and dry at room temperature;

[0054] (2) The ITO conductive glass treated in step (1) is used as the working electrode, the platinum wire is used as the counter electrode, and the silver / silver chloride electrode is used as the reference electrode, and the three electrodes are inserted into the electrode containing 0.02mol L -1 Copper sulfate and 0.05mol L -1 In the sodium hydroxide solution of sodium citrate, adjust the concentration of sodium hydroxide in the solution so that the pH value of the solution is 10;

[0055] (3) Under the condition that the deposition temperature is 50°C ...

Embodiment 3

[0062] Embodiment 3: a method for constructing a cuprous oxide film-based non-enzyme-oxygen sensitive glucose photoelectrochemical sensor, comprising the following steps:

[0063] (1) Cut the ITO conductive glass into a size of 1 cm × 4 cm, and contain 1mol L -1 Sodium hydroxide and 0.6% hydrogen peroxide cleaning solution for cleaning, after cleaning, use acetone and ultrapure water to rinse and dry at room temperature;

[0064] (2) The ITO conductive glass treated in step (1) is used as the working electrode, the platinum wire is used as the counter electrode, and the silver / silver chloride electrode is used as the reference electrode, and the three electrodes are inserted into the electrode containing 0.08mol L -1 copper sulfate and 0.2mol L -1 In the sodium hydroxide solution of sodium citrate, adjust the concentration of sodium hydroxide in the solution so that the pH value of the solution is 12;

[0065] (3) Electrodeposit for 30 minutes at a deposition temperature of ...

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Abstract

The invention discloses a construction method of a cuprous oxide membrane-based enzyme free-oxygen sensitive glucose photo electrochemical sensor. The construction method comprises the following steps: with an ITO conductive glass as a working electrode, a platinum wire as a counter electrode and a silver/ silver chloride electrode as a reference electrode, inserting the three electrodes into a sodium hydroxide solution containing 0.05mol/L copper sulfate and 0.1mol/L sodium citrate, and regulating a pH value to be 11; under the conditions that the deposition temperature is 60 DEG C, the deposition potential is -0.4V and the deposition time is 20 minutes, forming a cuprous oxide membrane on the surface of the ITO conductive electrode; and rinsing residual solution on the surface with pure water, drying for 1h at the temperature of 100 DEG C, and forming a stable cuprous oxide membrane-based photocathode. According to the construction method disclosed by the invention, photocurrent detection based on light excitation is low in detection signal background and can have high detection sensitivity and stability without needing expensive instrument and equipment and complex sample treatment.

Description

technical field [0001] The invention belongs to the field of instrument analysis and testing, in particular to a construction method and detection method of a photoelectrochemical sensor for glucose detection. Background technique [0002] Glucose is an important analyte in clinical diagnostics because its high concentration can lead to the development of diabetes. Currently, various related techniques such as infrared spectroscopy, fluorescence spectroscopy, and photoacoustic spectroscopy; surface plasmon resonance biosensors; capacitive detection; electrochemiluminescence; colorimetry; and capillary electrophoresis have been used Glucose detection. These methods generally require more expensive instrumentation and more complicated sample handling. [0003] Compared with the above analytical techniques, the enzyme-based electrochemical sensor shows high selectivity and high sensitivity, but its stability is poor because glucose oxidase is easily affected by pH, humidity, ...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 李红波李静王红梅王伟
Owner YANCHENG INST OF TECH
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