Enzyme-free glucose photoelectrochemical sensor, and detection method of enzyme-free glucose concentration

A glucose concentration and photoelectrochemical technology is applied in the field of glucose analysis and testing, which can solve the problems such as the difficulty of long-term fixation of glucose oxidase, the influence of glucose detection results, and the unsuitability for detection, and achieves low detection signal background, simplified sample processing, and low cost. Effect

Inactive Publication Date: 2019-01-18
HUBEI ENG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the glucose oxidase electrochemical sensor, which is widely studied, is limited by the physical and chemical properties of the enzyme, which makes it difficult for glucose oxidase to be fixed on the electrode for a long time, and is easily interfered by factors such as temperature and chemical environment. have an impact
In addition, although enzyme-free glucose sensors have attracted extensive research in recent years, most of these sensors are based on nanoparticles such as gold, platinum, copper, and their noble metal alloys, which have defects such as high cost, poor selectivity, and instability, and are not suitable for practical applications. Sample testing

Method used

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  • Enzyme-free glucose photoelectrochemical sensor, and detection method of enzyme-free glucose concentration
  • Enzyme-free glucose photoelectrochemical sensor, and detection method of enzyme-free glucose concentration
  • Enzyme-free glucose photoelectrochemical sensor, and detection method of enzyme-free glucose concentration

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Embodiment 1

[0039] A kind of WO 3 A method for preparing a photoanode, comprising:

[0040] a. Cut the FTO conductive glass into a size of 2 cm × 2.5 cm, first boil it in 5% sodium hydroxide solution, and then use acetone, ethanol and ultrapure water to ultrasonically clean the FTO;

[0041] b. Take 1.2mmolNa 2 WO 4 2H 2 O solution and 1.2mmol ammonium oxalate were dissolved in 33mL of deionized water, and 9mL of 36.5% hydrochloric acid was added to obtain a yellow tungstic acid precipitate. After stirring for 10 minutes, 8mL of 30% H 2 o 2 , the precipitate was dissolved and a clear peroxytungstic acid solution was obtained, then 30mL of ethanol was added and the stirring was continued for 10 minutes, then the cleaned conductive substrate was leaned against the wall of the beaker and placed in a water bath at 85°C for 150 minutes. During this process, A uniform tungstic acid film is slowly grown on the FTO substrate, and the above tungstic acid film is rinsed with deionized water an...

Embodiment 2

[0044] A kind of WO 3 A method for preparing a photoanode, comprising:

[0045] a. Cut the FTO conductive glass into a size of 2 cm × 2.5 cm, first boil it in 5% sodium hydroxide solution, and then use acetone, ethanol and ultrapure water to ultrasonically clean the FTO;

[0046] b. Take 0.5mmolNa 2 WO 4 2H 2 O solution and 0.5mmol ammonium oxalate were dissolved in 33mL of deionized water, and 5mL of 36.5% hydrochloric acid was added to obtain a yellow tungstic acid precipitate. After stirring for 10 minutes, 6mL of 30% H 2 o 2 , the precipitate was dissolved and a clear peroxytungstic acid solution was obtained, then 30mL of ethanol was added and the stirring was continued for 10 minutes, then the cleaned conductive substrate was leaned against the wall of the beaker and placed in a water bath at 75°C for 120 minutes. During this process, A uniform tungstic acid film is slowly grown on the conductive substrate, and the above tungstic acid film is rinsed with deionized w...

Embodiment 3

[0049] A kind of WO 3 A method for preparing a photoanode, comprising:

[0050] a. Cut the FTO conductive glass into a size of 2 cm × 3.5 cm, first boil it in 5% sodium hydroxide solution, and then use acetone, ethanol and ultrapure water to ultrasonically clean the FTO;

[0051] b. Take 1.5mmolNa 2 WO 4 2H 2 O solution and 1.5mmol ammonium oxalate were dissolved in 33mL of deionized water, and 10mL of 36.5% hydrochloric acid was added to obtain a yellow tungstic acid precipitate. After stirring for 10 minutes, 10mL of 30% H 2 o 2 , the precipitate was dissolved and a clear peroxytungstic acid solution was obtained, then 30mL of ethanol was added and stirring continued for 10 minutes, then the cleaned conductive substrate was leaned against the wall of the beaker and placed in a water bath at 85°C for 170 minutes. During this process, A uniform tungstic acid film is slowly grown on the conductive substrate, and the above tungstic acid film is rinsed with deionized water a...

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Abstract

The invention relates to an enzyme-free glucose photoelectrochemical sensor, and a detection method of enzyme-free glucose concentration, and belongs to the technical field of glucose analysis and test. The enzyme-free glucose photoelectrochemical sensor comprises a WO3 nanosheet array film electrode, and the WO3 nanosheet array film electrode is applied to a working electrode. Based on the photocurrent detection under photoexcitation, the enzyme-free glucose photoelectrochemical sensor has an excitation source and an electrochemical signal detection system, which are independent from each other, so that the detection signal background is low, the signal is strong, and higher detection sensitivity is provided without using expensive equipment.

Description

technical field [0001] The invention belongs to the technical field of glucose analysis and testing, and in particular relates to an enzyme-free glucose photoelectrochemical sensor and an enzyme-free glucose concentration detection method. Background technique [0002] The quantitative analysis of glucose plays an extremely important role in clinical medicine, biochemistry, environmental monitoring, food science and other fields. The development of glucose sensors has always been the focus of the development of the sensor field. At present, the determination methods of glucose mainly include infrared spectroscopy, capillary electrophoresis, fluorescence spectroscopy, photoacoustic spectroscopy, colorimetry, surface plasmon resonance biosensor and electrochemiluminescence. However, these methods usually require more expensive equipment and more complicated sample processing, which is not conducive to popularization. [0003] In contrast, electrochemical biosensors have great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 张丙青余世桨苏宏晖程凡胡富强钟菲
Owner HUBEI ENG UNIV
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