Enzyme-free glucose electrochemical sensor and detection method thereof

A glucose and electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve tedious, time-consuming, limited, and limited problems, and achieve good electrode stability, broad application prospects, and good repeatability Effect

Active Publication Date: 2018-11-13
SOUTHEAST UNIV
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Glucose detection usually includes two methods: enzyme detection and non-enzyme detection. Among them, enzyme detection is the main method for in vitro detection of glucose. By adding highly specific glucose oxidase or dehydrogenase during the detection process, a large amount of glucose can be eliminated. However, glucose oxidase is not stable, its catalytic activity is affected by ambient temperature, humidity and pH, and its stability is tested with the increase of storage time, although it has been Many methods have been reported to improve the stability of enzymes, but on the one hand, these operations are tedious and time-consuming, on the other hand, the repe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme-free glucose electrochemical sensor and detection method thereof
  • Enzyme-free glucose electrochemical sensor and detection method thereof
  • Enzyme-free glucose electrochemical sensor and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 As shown, a gold electrode (2mm cross-sectional diameter) is used as the working electrode 1, silver-silver chloride is used as the reference electrode 2, and platinum wire is used as the counter electrode 3 to form a three-electrode system. In 0.1M pH 5.0 phosphate buffer, the glucose in the buffer was detected using an electrochemical workstation. Select multi-potential detection in the technology menu bar in the settings, and set the potentials that need to be applied in sequence in the parameter settings. In this test, the three potential settings are: apply -2.0V potential for 24s, +0.2V potential for 5s, and +1.0V potential for 2s. The detection is a cyclic process. During the detection process, the glucose concentration is changed to obtain the relationship between the glucose oxidation current and the concentration. , the detection current has a linear relationship with the glucose concentration in the range of 10 μM to 1025 μM.

Embodiment 2

[0033] A platinum electrode (2mm cross-sectional diameter) was used as the working electrode, silver-silver chloride was used as the reference electrode, and platinum wire was used as the counter electrode to form a three-electrode system. Glucose in the test. Use a pipette to draw 2.5 μL of 0.5% perfluorosulfonic acid solution and drop it on the surface of the platinum electrode, and let it dry naturally. The detection operation process is similar to Example 1. In this detection, the three potential settings are: -1.8V, 20s; +0.1V, 5s; +1.0V, 5s. The detection current has a linear relationship with the glucose concentration in the range of 30 μM to 750 μM.

Embodiment 3

[0035] A copper-nickel alloy (copper: nickel = 1:5) electrode (2mm diameter) is used as the working electrode, a saturated calomel electrode is used as the reference electrode, and a platinum wire is used as the counter electrode to form a three-electrode system. In 0.1M pH 7.0 phosphate buffer In , the glucose in the buffer was detected using an electrochemical workstation. Select the current-time curve in the technical menu bar of the setting, set the starting point as the detection potential +0.6V in the parameter setting, and set the time as 5s. Select pretreatment in the control menu bar, and set two pretreatment potentials in it, the potential 1 is +0.8V, and the time is 5s, which is used to remove the oxidation product adsorbed in the previous detection, and the potential 2 is set to -1.8V , the time is 20s. Each time the glucose concentration is changed, the detection is repeated, and finally the relationship between the detection current and the glucose concentration...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an enzyme-free glucose electrochemical sensor and a detection method thereof. The enzyme-free glucose electrochemical sensor comprises a three-electrode system consisting of areference electrode, a counter electrode and a working electrode, wherein the working electrode is made of a material with electrocatalytic oxidation performance for glucose; the method for detectingglucose by using the enzyme-free glucose electrochemical sensor comprises the following steps: (1) electrochemical pretreatment of the working electrode: applying high negative potential; (2) electrochemical oxidation of glucose: applying the potential required for glucose oxidation; (3) electrochemical cleaning of the working electrode: applying positive potential. The influence of pH of a sampleon a detection result can be removed, enzyme-free glucose detection originally required under the alkaline condition can be performed in neutral and acidic samples, and the sensor has the advantagesof good electrode stability, high sensitivity, good repeatability and the like, and has a very broad application prospect.

Description

technical field [0001] The invention relates to a glucose detection device and a detection method thereof, in particular to an enzyme-free glucose electrochemical sensor and a detection method thereof. Background technique [0002] Glucose is the most widely distributed and most important monosaccharide in nature, and it plays an important role in the field of biology. The development of high-sensitivity glucose detection technology is of great significance to the study of biological metabolism and diagnosis of diseases. Electrochemical detection method has attracted more and more attention due to its high sensitivity, simple operation, easy miniaturization and real-time online. Glucose detection based on electrochemical technology has also become a hot research topic for researchers. [0003] Glucose detection usually includes two methods: enzyme detection and non-enzyme detection. Among them, enzyme detection is the main method for in vitro detection of glucose. By adding...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/31G01N27/327G01N27/38
CPCG01N27/31G01N27/3277G01N27/3278G01N27/38
Inventor 刘宏朱小飞鞠寅晖陈键刘德晔
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap