Enzyme-free potentiometric glucose sensor and detection method thereof

A glucose sensor and potentiometric technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of cumbersome operation, high cost, unsuitable rapid detection of glucose, etc., and achieves the effects of low cost, simple production, and easy miniaturization.

Inactive Publication Date: 2015-05-06
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, many boric acid-based glucose sensors have been developed. In addition to using boric acid derivatives as glucose recognition molecules, such sensors usually require a reporter molecule to conduct the recognition process; although some senso...

Method used

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  • Enzyme-free potentiometric glucose sensor and detection method thereof
  • Enzyme-free potentiometric glucose sensor and detection method thereof
  • Enzyme-free potentiometric glucose sensor and detection method thereof

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Embodiment 1

[0028] The present invention uses [methylene-bis(3,1-phenylaminomethyl-2,1-phenyl)] diboronic acid (see figure 2 ) is used as an example to detect glucose as a recognition molecule, which can be prepared according to the description in Document 5. The operation steps are as follows:

[0029] (1) Preparation of polymer membrane electrodes

[0030] Weigh 196mg of polyvinyl chloride, 196mg of o-nitrophenyloctyl ether, and 4.54mg of tridodecylmethylammonium chloride, dissolve them in 3.5mL of tetrahydrofuran, stir them evenly, and pour them into a glass plate with an inner diameter of 3.5cm. in the glass ring. After the tetrahydrofuran was volatilized, the membrane was cut into small discs with an inner diameter of 3 mm using a puncher and pasted on the tip of a pipette with a PVC tube at the bottom. Use 10mM NaCl solution as the inner filling solution and activation solution to activate the electrode, ready for use.

[0031] (2) Optimization of pH conditions

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Abstract

Belonging to the technical field of biochemical analytical test, the invention specifically relates to an enzyme-free potentiometric glucose sensor with high selectivity and a detection method thereof. A boric acid derivative containing two boric acid groups is used as a glucose recognition molecule, and a quaternary ammonium salt doped polymer is taken as the electrode. The sensor provided by the invention is employed to detect the concentration of glucose in a whole blood sample, and the obtained result is consistent with that of commercially available glucometers.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis and testing, and in particular relates to a high-selectivity, enzyme-free potentiometric glucose sensor and a detection method thereof. Background technique [0002] Sugar has important physiological significance in the life activities of the human body, and abnormal blood sugar concentration is often used as a sign of metabolic disorders, so the detection of blood sugar is of great significance. In clinical analysis and actual operation, blood glucose detection is generally completed with the aid of a blood glucose meter. The blood glucose meter uses glucose oxidase to recognize and catalyze the oxidation of glucose to generate electroactive hydrogen peroxide, and uses a miniaturized electrode to detect the redox current of hydrogen peroxide, which indirectly realizes the detection of glucose. Although such sensors usually have good selectivity and sensitivity, due to the variabilit...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/416
Inventor 秦伟李龙陈红朵
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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