Method for signal amplification fluorescence detection of aflatoxin B1 based on hybridization chain reaction and DNAzyme

A signal amplification and aflatoxin technology, which is applied in the fields of analytical chemistry and biosensing detection, can solve the problems of troublesome pretreatment of graphene oxide, harsh environment required for the reaction system, and failure to achieve signal amplification, etc., to achieve high Sensitivity and selectivity, high actual sample detection ability, effect of improving detection sensitivity
CN107955830AInactive Publication Date: 2018-04-24OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2018-04-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for detecting aflatoxin B1 based on hybridization chain reaction and DNAzyme signal amplification fluorescence detection, the invention relates to the construction and application of a signal amplification technology fluorescence spectrum sensor. Using the target aflatoxin B1 to trigger a change in the conformation of the aptamer DNA, promote the assembly of the hairpin probe to form a long-chain repetitive DNAzyme unit, and catalyze the enzyme-free AFB1 sensing strategy that cuts the molecular light to generate a fluorescent signal. Relying on this method, the detection limit can reach 0.5 ng / mL, which is far lower than the national standard (10 ng / mL). In addition, we tested aflatoxin B1 in the moldy rice extract and also obtained satisfactory results.
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Description

technical field

[0001] The invention belongs to the field of analytical chemistry and biosensing detection, and relates to the construction and application of a signal amplification technology fluorescence spectrum sensor. In addition, the present invention also relates to a method for detecting aflatoxin B1 by using the fluorescent spectrum sensor. Background technique

[0002] Aflatoxin is a mycotoxin produced by Aspergillus flavus, A. It is classified as the first carcinogen by the International Agency for Research on Cancer (IARC). AFB1 widely exists in many contaminated foods, such as grains, beer, spices and their additives, nuts and beans. Many countries have stipulated the content standard of AFB1 in crop products. The European Union stipulates that the content of aflatoxin B1 in food should not exceed 2 ppb. Therefore, the control detection of aflatoxin B1 content is of great significance to human health diet.

[0003] Traditional methods for the detection of a...

Claims

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