22 results about "Signal amplification technique" patented technology

The invention relates to the field of veterinary drug residue detection, in particular to a fluorescence sensor based on DNA molecular machine mediated split Cas12a and application of the fluorescence sensor to ofloxacin detection, the fluorescence sensor comprises a system A, a system B and a system C. According to the final concentration of each system, the system A comprises an AP / CP double-stranded compound and a 20 mM Tris-HCl buffer solution 1, the system B comprises a double-stranded compound, an sRNA probe and a 10 mM Tris-HCl buffer solution 2, and the system C comprises a DNA molecular machine mediated split Cas12a. The system C comprises a Cas12a / hRNA compound, a pDNA / psG43 signal probe, ThT and a 20 mM Tris-HCl buffer solution; aP in the AP / CP double-chain compound is an aptamer of ofloxacin, and AP and CP in the AP / CP double-chain compound are paired and connected through complementary base pairing; the sequences of AP, CP, PS, SS, sRNA, hRNA, pDNA and psG43 are respectively as shown in SEQ ID NO.1-SEQ ID NO.8. The invention also discloses a kit for detecting the content of the protein. According to the application, the nucleic acid aptamer of ofloxacin is utilized, based on a DNA molecular machine and SCas12a as a signal amplification technology and sulfo-modified G43 as a fluorescent signal probe, even if the content of ofloxacin in a sample is extremely low, detection can be accurately realized.

This invention relates to the field of biomedical detection technology, and discloses a bitetrahedral framework nucleic acid TDN cascade amplification system and its application, including TDN-1 and TDN-2. TDN-1 has a hairpin H1 containing a fluorescent reporter group attached to its surface, and TDN-2 has a hairpin H2 attached to its surface. The sequences of H1 and H2 are designed to undergo an alternating hybridization chain reaction triggered by target mRNA. This scheme relies on the synergistic effect of TDN structural anchoring and precise hairpin probe sequence design to overcome the technical defects of traditional free HCR hairpins in living cells, such as poor stability, low reaction efficiency, and non-specific triggering, achieving more efficient and specific intracellular signal amplification. Furthermore, it enables highly specific and sensitive in-situ imaging and dynamic tracking of low-abundance mRNAs such as c-Myc in single living cells, effectively solving the problems of insufficient detection sensitivity and false positives caused by the inability of traditional detection methods to effectively enrich targets and the low efficiency and high background of traditional HCR and other signal amplification technologies in intracellular applications.

The present application discloses a vector for preparing a DNA detection probe, a construction method thereof and an application thereof. The vector is a Pt / Ti3C2T x nanoparticle, which is prepared by reducing and loading platinum nanoparticles using the two-dimensional material Ti3C2T x without the need to additionally add a reducing agent. The Pt / Ti3C2T x nanoparticle has a relatively dark initial color and has good peroxidase-like catalytic activity. Therefore, it can be introduced as a bifunctional signal tag into a lateral flow chromatography biosensor, and this signal tag adopts an aggregation signal amplification technique and a catalytic signal amplification technique to improve the sensitivity and accuracy of the lateral flow test strip biosensor. In addition, based on the Pt / Ti3C2T x nanoparticle as a signal carrier, a probe is prepared by labeling SH-DNA through Pt-S bonds, which can be applied to the detection of HIV-DNA, and can obtain detection results earlier, which is beneficial to the treatment of patients and the effective control of the virus.

The present invention provides a detection method and application of a ratio-type fluorescence sensor for detecting OTA based on CHA. A ratio-type fluorescence sensor is constructed by using CDs and FAM as dual-emission fluorescence probes in combination with the CHA signal amplification technology. Finally, the ratio F FAM / F CDs is used to achieve the quantitative detection of ochratoxin A, with a linear range of 5.0 pg / mL - 3.0 ng / mL and a detection limit of 1.5 pg / mL. The ratio-type fluorescence sensor for detecting ochratoxin A in the present invention not only has a self-calibration function, can eliminate the interference of various variable or difficult-to-quantify factors such as probe concentration, polarity, temperature, and excitation intensity, broaden the response range, and effectively improve the accuracy and reliability of the detection results, but also does not involve enzymes such as proteases in the whole operation process, is less affected by the external environment, has high sensitivity, low requirements for operating conditions and operating costs, can achieve the accurate detection of ultra-trace OTA, and has the value of industrial application and popularization.

The invention relates to the technical field of signal amplification, in particular to a signal amplifier of a pressure-resistant hydrophone, and the signal amplifier comprises a shell which comprises an outer shell and an inner shell, the outer shell is of an annular hollow structure, the outer shell and the inner shell are fixedly connected through a pressure-resistant mechanism, and the pressure-resistant mechanism is annularly arranged between the outer shell and the inner shell; a cushioning mechanism is further arranged between the outer shell and the inner shell, the cushioning mechanism is annularly arranged between the outer shell and the inner shell, the sealing mechanism is located in the inner shell and the outer shell, the amplifying mechanism is located in the inner shell, and the amplifying mechanism and the inner shell are connected in a matched mode through the sealing mechanism; the stability of the structure under the dynamic load is enhanced.

The invention provides a detection chip, device and method for detecting HIV RNA in saliva, the detection chip comprises a chip substrate, a plurality of electric signal transmission interfaces are integrated on the chip substrate, the detection chip further comprises a reaction electrode, a differential electrode and a reference electrode, the surface of the reaction electrode is modified with nucleic acid aptamer molecules, and the surface of the differential electrode is modified with nucleic acid aptamer molecules. The detection module is used for generating a detection electric signal according to specific binding of nucleic acid aptamer molecules and HIV RNA; the differential electrode is a blank electrode and is used for deducting background signals generated by non-specific molecules in an added sample solution and a to-be-detected solution and amplifying a detection electric signal through the differential amplification circuit; the reference electrode is used for providing stable reference potential; the reaction electrode, the differential electrode and the reference electrode are electrically connected with an electric signal transmission interface respectively. According to the invention, the HIV RNA in the saliva is detected based on field effect transistor biological detection and by adopting a differential signal amplification technology, so that the effects of accurately detecting trace HIV RNA in the saliva, improving the yin-yang coincidence rate and simplifying the detection operation are achieved.

The invention relates to a specific probe for a branched DNA amplification system and application of the specific probe, and belongs to the technical field of pathological diagnosis. On the basis of an original target gene probe, a pre-amplification probe, an amplification probe and a marking probe of a branched chain DNA amplification system probe system, a closing probe for closing the pre-amplification probe is added, and the closing probe can be complementarily combined with the pre-amplification probe. Probes used in the branched DNA signal amplification (bDNA) technology are all single-stranded DNA (ssDNA), and the combination of a closed probe and a pre-amplification probe can reduce the combination of non-target fragments in a sample and the ssDNA, so that the non-specific hybridization is reduced, and the specificity of the bDNA is improved.

The invention relates to a semeglutide electrochemiluminescence immunoassay method based on a TSA signal amplification technology and a kit, and solves the technical problem of how to detect semeglutide with simple and rapid operation, wide detection range and high detection sensitivity. The method is simple to operate, can quickly detect the semeglutide, and is wide in detection range and high in detection sensitivity.

The invention relates to a proximity induction amplification system for efficiently visualizing sialic acid modified RNA and application of the proximity induction amplification system. The proximity induction amplification system comprises a molecular recognition module, a regulation and control module and a signal enhancement module, the molecular recognition module comprises an RNA recognition probe and a sialic acid recognition probe; the regulation and control module comprises a locking chain and an unlocking chain; and the amplification system comprises a probe H1 and a probe H2. According to the invention, through metabolic labeling and orthogonal chemical reaction and in combination with an HCR signal amplification technology, efficient and sensitive detection of sialylated RNA on a cell membrane is realized. The method has the advantages of simplicity and convenience in operation, good signal amplification effect, wide application range and the like, and can be widely applied to biomedical research and clinical diagnosis.

The invention relates to the technical field of electrical detection, in particular to a multilayer adaptive ripple recognition method based on wavelet decomposition and related equipment. The method comprises the following steps: acquiring a high-voltage signal; the obtained high-voltage signal is preprocessed; performing signal amplification and digital processing on the preprocessed high-voltage signal through a signal amplification technology and an analog-to-digital conversion technology to obtain a preliminary digital signal; processing the preliminary digital signal through a wavelet decomposition algorithm to obtain direct current ripple data; performing feature extraction on the direct current ripple data through a wavelet basis to obtain an insulation state feature; and comparing the insulation state characteristics with a self-adaptive threshold value, and starting an early warning operation according to a comparison result. According to the method, the number of layers of wavelet decomposition and an analysis frequency band can be adaptively selected, energy evaluation and frequency domain positioning are carried out on decomposed detail components of each layer, effective layers containing ripple characteristics are dynamically identified, and online detection, alarm and data uploading are realized.

The invention belongs to the technical field of molecular biology, and discloses a preparation method of a fluorescence in situ hybridization kit based on a hybridization chain reaction (HCR), the core components of the kit comprise a hybridization probe, an amplification probe, a washing liquid, a confining liquid, protease K, HRP-mouse anti-digoxin, and a TSA developing liquid 520 nm. According to the FISH detection system combined with the HCR signal amplification technology, when a hybridization probe and an amplification probe are simultaneously combined to a position adjacent to a target sequence, a small section of sequence at the top end can trigger a branched HCR reaction to form a macromolecular nucleic acid aggregate. Signal amplification of HCR is achieved through cascade hybridization reaction, detection sensitivity and specificity can be remarkably improved, and meanwhile the problem of background noise caused by a traditional enzymatic method is avoided. The kit disclosed by the invention is wide in sample application range, and can be used for positioning analysis of target RNA of samples such as paraformaldehyde or formalin-fixed animal and plant tissues, paraffin or frozen sections, cells and the like, and high-sensitivity detection of the single cell level of non-coding RNA.

The application provides a urine double-miRNA detection method based on multiple signal amplification and metal ion selective recognition phenomenon and application thereof, and relates to the technical field of biomedical diagnosis. 2+ and Ag + , which are respectively reacted with CDs and CdTe QDs, and finally, the fluorescence signals of CDs and the fluorescence signals of CdTe QDs are used for quantifying miRNA-375 and miRNA-148a. The application establishes a simple, rapid, sensitive and simultaneous homogeneous urine double-miRNA quantitative analysis strategy from the aspects of analysis instrument, analysis steps and cost, selection of signal reporting molecules, multiple nucleic acid signal amplification technology and application of selective recognition phenomenon.

The invention discloses a low-permeability reservoir fractured well rapid well test analysis method based on a small signal amplification technology. The method comprises the steps that an early analytical solution of a low-permeability reservoir fractured well trilinear flow model is obtained; obtaining an analytical solution and an early analytical solution of the infinite flow guide fracture model of the fractured well of the low-permeability reservoir; constructing a bottom hole pressure influence function of the fractured well of the low-permeability reservoir; constructing an analytical solution of a finite flow guide fracture model of the fractured well of the low-permeability reservoir, and calculating the bottom-hole theoretical pressure of the fractured well of the low-permeability reservoir; obtaining an early analytical solution of the low-permeability reservoir finite flow guide fracture model, and further constructing a pressure-pressure derivative difference function based on a small signal amplification technology; and developing a new double logarithmic curve chart for rapid well testing of the fractured well of the low-permeability reservoir, explaining pressure measurement data of the fractured well of the low-permeability reservoir, and obtaining reservoir parameters. The method provided by the invention is simple and easy to understand, simple to operate, rapid in calculation, capable of effectively reducing the multiplicity of well test interpretation of the pressure measurement data of the low-permeability reservoir fractured well, and wide in application prospect.

The application discloses a position consistent control system of multi-four-rotor aircraft based on attack isolation and privacy protection and belongs to the technical field of industrial process control; the position consistent control system comprises a Liu password system, a network attack detection and isolation system and a consistent control system; the Liu password system performs encrypted transmission on provided leader expected signals and position information and obtains decrypted information; the network attack detection and isolation system detects attacked followers and isolates the attacked followers; input ends of the consistent control system are connected with output ends of an undirected graph G; and output ends of the consistent control system are connected with input ends of the i-th follower. The signal amplification technology of the Liu password system is used to protect the sensitivity of information and reduce errors between plaintext encryption and decryption; the consistent controller is used to compensate unknown dynamics and errors between real signals and decrypted signals through a small number of learning parameters, so that the protection of sensitive information and network security of the multi-four-rotor aircraft during task execution are ensured.

The invention belongs to the field of biological nucleic acid detection, and discloses a G4-crRNA / AZD < + + >-based CRISPR (clustered regularly interspaced short palindromic repeats) light-controlled tubular detection kit, which comprises the following components: G4-crRNA / AZD < + + >, Cas12a protease, an RPA (recombinase polymerase) isothermal amplification reagent, a buffer solution and a fluorescence report probe. The kit can obviously improve the detection sensitivity of a target, is high in specificity, effectively shortens the detection time, and is high in universality. The invention further discloses a corresponding detection method, the activity of the CRISPR system is started in a light-operated mode by combining a nucleic acid amplification signal amplification technology, the problem that the nucleic acid amplification efficiency is reduced due to cis-cleavage and trans-cleavage of Cas protease is avoided, rapid detection of targets in various samples can be achieved, and the detection accuracy is improved. A basis is provided for establishing a detection method which is low in cost, high in simplicity, strong in universality, high in sensitivity and good in specificity.

The invention relates to the technical field of electronic detection and signal amplification, in particular to a high-performance multi-channel arc channel type electron multiplier, which comprises an electronic input end used for receiving and focusing incident particles; the partition area is used for establishing a potential gradient; the alloy coating electrodes are respectively arranged at the input end and the output end of the electron multiplier and are used for applying working voltage; the multi-channel arc-shaped array structure comprises a plurality of arc-shaped channels which take the center of the section of the electron multiplication area tube body as the circle center and are uniformly arranged on the same circumference, and the arc-shaped channels are parallel to one another; the voltage division area is used for stabilizing electric field distribution in the channel; compared with the prior art, the high-gain and high-resolution collaborative improvement is realized, the high-temperature-resistant stability is excellent, the working life is ultra-long, meanwhile, low noise, quick response and efficient signal detection are achieved, the performance reaches the import standard, and a reliable core component is provided for localization of high-end instruments.

The invention relates to the technical field of biomedical detection, and discloses a gene marker for evaluating the risk of congenital heart disease related pulmonary hypertension and application, the gene marker is S100A3, and the expression level of the gene marker can evaluate the risk of congenital heart disease related pulmonary hypertension. According to the present invention, the detection sensitivity and the clinical prediction value can be significantly improved by combining the specific antibody, the nanometer signal amplification technology and the multi-omics data analysis.

The utility model provides an FM + DAB signal amplification circuit and electronic equipment thereof, and relates to the technical field of signal amplification, the circuit comprises a receiving port antenna input port, an amplifier circuit, a power divider circuit, an LC band elimination filter circuit and an output module, a receiving port is electrically connected with the input end of the amplifier circuit, and the output module is electrically connected with the input end of the power divider circuit. The output end of the amplifier circuit is connected with the input end of the power divider circuit, the output end of the power divider circuit is connected with the input end of the LC band-stop filter circuit, the output end of the LC band-stop filter circuit is connected with the output module, and the receiving port is configured to receive a DAB / FM signal. According to the amplifying circuit, an LCR is used for building a power dividing circuit, an LC filter circuit is used, and the signal amplifying requirement of two frequency bands of two receivers is met through one amplifying circuit.

The invention provides an intelligent electric meter data analysis device and method, and the device comprises a signal input module which is used for inputting a power line carrier signal of an intelligent electric meter; the signal separation module is provided with a filter circuit and is used for filtering a low-frequency power signal from the power carrier signal and outputting a high-frequency data signal; the signal enhancement module is provided with an amplification circuit and is used for performing gain amplification on the high-frequency data signal; and the optocoupler isolation module is used for separating the high-frequency data signal subjected to gain amplification into a level signal and transmitting the level signal to the control unit, so that the control unit identifies and analyzes the level signal and outputs analysis data. The hardware circuit of the embodiment adopts a universal device, so that the use of a high-cost special chip is avoided, and meanwhile, the circuit is simple in structure and easy to produce and maintain. Meanwhile, through the optical coupling isolation and signal amplification technology, the signal quality is remarkably improved, and the signal strength requirement and compatibility with the intelligent electric meter are ensured.

The invention belongs to the field of molecular biology, and particularly relates to an AFB1 detection method based on SERS fusion HCR signal amplification technology. According to the invention, gold-coated magnetic nanoparticles (Fe3O4-coated AuNPs) are established as a substrate, and the trace AFB1 is detected on the basis of a strategy of fusing a surface enhanced Raman spectroscopy (SERS) with a hybridization chain reaction (HCR). According to the invention, the specific gene sequence is modified on the gold-coated magnetic nanoparticles to be used as an identification probe of the AFB1, and methylene blue is used as a signal molecule to be embedded between DNA double chains, so that the conversion and amplification efficiency in AFB1 detection is greatly improved; meanwhile, the method does not need to depend on expensive equipment and expensive materials, and has the advantages of high efficiency, environmental protection, simplicity, convenience, high sensitivity and the like; the method has good selectivity and reproducibility, has huge potential in research of AFB1 detection, and has a wide market application prospect.

The application discloses a primer for rapidly detecting fish rhabdovirus CAPRV2023 on site based on double signal amplification technology and application thereof, and the primer comprises a DNA forward primer F2, a DNA reverse primer R2 and an RNA primer RNA4; the sequences of the DNA forward primer F2, the DNA reverse primer R2 and the RNA primer RNA4 are respectively shown as SEQ ID NO:2, SEQ ID NO:14 and SEQ ID NO:10; the 5' end of the RNA primer RNA4 is a FAM-labeled reporter group, and the 3' end is a BHQ1-labeled quencher group. The application further discloses application of the primer or kit in rapid detection of fish rhabdovirus CAPRV2023 on site for non-disease diagnosis purposes. The kit can efficiently and rapidly detect samples, has high specificity, and can early discover samples infected with CAPRV2023.