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33 results about "Signal amplification technique" patented technology

Tyramide substrates for HRP (TSA and Other Peroxidase-Based Signal Amplification Techniques—Section 6.2, ) and our ELF substrates for alkaline phosphatase (Phosphatase-Based Signal Amplification Techniques—Section 6.3, ) fulfill this requirement.

Vibration cylinder pressure sensor signal amplification circuit and vibration cylinder pressure sensor

The invention belongs to the technical field of sensor signal amplification, and discloses a vibration cylinder pressure sensor signal amplification circuit and a vibration cylinder pressure sensor.The vibration cylinder pressure sensor signal amplification circuit comprises a filter circuit, a gain adjusting circuit, a phase adjusting circuit, an amplitude limiting circuit, a frequency detection circuit and a temperature detection circuit; amplitude adjustment of a certain gain is achieved through a gain adjusting circuit, a gain amplification signal VB is obtained and input to a phase adjusting circuit and a frequency detection circuit, the phase adjusting circuit outputs a square wave signal VC and inputs the square wave signal VC to an amplitude limiting circuit, and the amplitude limiting circuit outputs an amplitude limiting square wave signal VD and loads the amplitude limiting square wave signal VD to a vibration cylinder; a gain amplification signal VB output by the gain adjusting circuit passes through the frequency detection circuit, a square wave frequency signal VO is output, a closed-loop control system is formed to achieve driving and detection of the resonance cylinder pressure sensor, and the actual frequency of the vibration cylinder body depends on the sensed pressure.
Owner:XIAN MICROELECTRONICS TECH INST

Single bead capacitive detector for microbial applications

The present invention provides an improved capacitive bead sensor for detecting and / or quantifying a target analyte in a sample wherein the detection limit is as low as a single bead, the capacitive bead sensor can be repeatedly used for multiple bead testing or continuous bead flow, and the capacitive bead sensor is readily manufacturable and automatable. By using various structural advantages and electronic signal amplification techniques, the capacitive bead sensor enables sensitivity as low as single molecule detection without the need for enzymatic amplification such as PCR, these various structural advantages and electronic signal amplification techniques further allow for multiplex target detection not only across various nucleic acid targets but across the entire target class, thereby allowing for simultaneous detection of, for example, viral nucleic acids and host antibodies against these viruses.
Owner:ALTRATECH LTD

Primer for rapidly detecting fish rhabdovirus CAPRV2023 on site based on dual signal amplification technology and application of primer

The invention discloses a primer for rapidly detecting fish rhabdovirus CAPRV2023 on site based on a dual signal amplification technology and application of the primer, the primer comprises a DNA forward primer F2, a DNA reverse primer R2 and an RNA primer RNA4, the sequences of the DNA forward primer F2, the DNA reverse primer R2 and the RNA primer RNA4 are respectively as shown in SEQ ID NO: 2, SEQ ID NO: 14 and SEQ ID NO: 10, the 5'end of the RNA primer RNA4 is an FAM labeled reporter group, and the 3 'end of the RNA primer RNA4 is a BHQ1 labeled quenching group. The invention also discloses an application of the primer or the kit in on-site rapid detection of the fish rhabdovirus CAPRV2023 with a non-disease diagnosis purpose. The kit disclosed by the invention can be used for efficiently and quickly detecting a sample, is high in specificity and can be used for discovering the sample infected with CAPRV2023 as soon as possible.
Owner:GUANGDONG OCEAN UNIVERSITY

Fluorescence sensor based on DNA molecular machine mediated split Cas12a and application of fluorescence sensor in ofloxacin detection

The invention relates to the field of veterinary drug residue detection, in particular to a fluorescence sensor based on DNA molecular machine mediated split Cas12a and application of the fluorescence sensor to ofloxacin detection, the fluorescence sensor comprises a system A, a system B and a system C. According to the final concentration of each system, the system A comprises an AP / CP double-stranded compound and a 20 mM Tris-HCl buffer solution 1, the system B comprises a double-stranded compound, an sRNA probe and a 10 mM Tris-HCl buffer solution 2, and the system C comprises a DNA molecular machine mediated split Cas12a. The system C comprises a Cas12a / hRNA compound, a pDNA / psG43 signal probe, ThT and a 20 mM Tris-HCl buffer solution; aP in the AP / CP double-chain compound is an aptamer of ofloxacin, and AP and CP in the AP / CP double-chain compound are paired and connected through complementary base pairing; the sequences of AP, CP, PS, SS, sRNA, hRNA, pDNA and psG43 are respectively as shown in SEQ ID NO.1-SEQ ID NO.8. The invention also discloses a kit for detecting the content of the protein. According to the application, the nucleic acid aptamer of ofloxacin is utilized, based on a DNA molecular machine and SCas12a as a signal amplification technology and sulfo-modified G43 as a fluorescent signal probe, even if the content of ofloxacin in a sample is extremely low, detection can be accurately realized.
Owner:LESHAN NORMAL UNIV

Satellite amplification communication method, system and device for emergency scene and medium

The invention discloses a satellite amplification communication method, system and device for an emergency scene and a medium, and relates to the technical field of satellite uplink signal amplification, and the method comprises the following steps: obtaining original reference data of a power amplifier, carrying out the preliminary screening processing, calculating cloud comprehensive features, cloud structure features and meteorological cooperation features, and carrying out the calculation of the cloud comprehensive features, the cloud structure features and the meteorological cooperation features; constructing second reference data; constructing an amplification feature vector, and constructing a power amplification processing model based on the second reference data; acquiring an amplification feature vector in real time, and dynamically adjusting the amplification power based on a power amplification calculation model; the satellite uplink signal amplification method is used for solving the problem that when an existing satellite uplink signal amplification technology carries out power amplification on a transmitting signal through a power amplifier, the relation among main meteorological factors, distance factors and signal loss cannot be fully considered, meanwhile, a relation model is established, the amplification power of the power amplifier is automatically and accurately adjusted according to the actual situation, and the reliability is high. And the problems of power waste and insufficient power are avoided.
Owner:CHONGQING HUAXIU TECH CO LTD

A Bi-Tetrahedral Framework Nucleic Acid TDN Cascade Amplification System and Its Application

This invention relates to the field of biomedical detection technology, and discloses a bitetrahedral framework nucleic acid TDN cascade amplification system and its application, including TDN-1 and TDN-2. TDN-1 has a hairpin H1 containing a fluorescent reporter group attached to its surface, and TDN-2 has a hairpin H2 attached to its surface. The sequences of H1 and H2 are designed to undergo an alternating hybridization chain reaction triggered by target mRNA. This scheme relies on the synergistic effect of TDN structural anchoring and precise hairpin probe sequence design to overcome the technical defects of traditional free HCR hairpins in living cells, such as poor stability, low reaction efficiency, and non-specific triggering, achieving more efficient and specific intracellular signal amplification. Furthermore, it enables highly specific and sensitive in-situ imaging and dynamic tracking of low-abundance mRNAs such as c-Myc in single living cells, effectively solving the problems of insufficient detection sensitivity and false positives caused by the inability of traditional detection methods to effectively enrich targets and the low efficiency and high background of traditional HCR and other signal amplification technologies in intracellular applications.
Owner:CHONGQING UNIV

Position consistency control system of multi-four-rotor aircraft based on attack isolation and privacy protection

The invention discloses a multi-four-rotor aircraft position consistency control system based on attack isolation and privacy protection, and belongs to the technical field of industrial process control. Comprising a Liu password system, a network attack detection and isolation system and a consistency control system, and the Liu password system carries out encryption transmission on a provided leader expected signal and position information and obtains decryption information; the network attack detection and isolation system detects and isolates attacked followers, the input end of the consistency control system is connected with the output end of the undirected graph G, and the output end of the consistency control system is connected with the input end of the ith follower. Through the signal amplification technology of the Liu password system, the sensitivity of information is protected, and the error between plaintext encryption and decryption is reduced; and the consistent controller compensates unknown dynamic states and errors between real signals and decryption signals through a small number of learning parameters, and ensures protection of sensitive information and network security when the multi-four-rotor aircraft executes tasks.
Owner:NANJING UNIV OF POSTS & TELECOMM

In-vitro detection or cell imaging method for N4-acetylcytosine (ac4C) RNA

The invention discloses an in-vitro detection or cell imaging method for N4-acetylcytosine (ac4C) RNA (Ribonucleic Acid), and belongs to the technical field of molecular biology and biological imaging. According to the method, ac4C RNA is marked through fluorine metabolism to generate F-ac4C RNA, an ac4C specific probe and an RNA sequence specific probe are introduced, and efficient and specific in-vitro detection and in-situ imaging of ac4C RNA loci of cells are realized by combining a signal amplification technology of rolling circle amplification reaction (RCA). The method is suitable for in-vitro detection and cell imaging of specific ac4C sites of multiple types of RNA (such as rRNA, mRNA, lncRNA and tRNA) and multiple types of cells. The invention is expected to provide a new way for revealing ac4C modified biological functions and mechanisms, and plays an important role in diagnosis and treatment of RNA related diseases.
Owner:ZHONGNAN HOSPITAL OF WUHAN UNIV

Preparation method and application of a DNA detection probe carrier

The present application discloses a vector for preparing a DNA detection probe, a construction method thereof and an application thereof. The vector is a Pt / Ti3C2T x nanoparticle, which is prepared by reducing and loading platinum nanoparticles using the two-dimensional material Ti3C2T x without the need to additionally add a reducing agent. The Pt / Ti3C2T x nanoparticle has a relatively dark initial color and has good peroxidase-like catalytic activity. Therefore, it can be introduced as a bifunctional signal tag into a lateral flow chromatography biosensor, and this signal tag adopts an aggregation signal amplification technique and a catalytic signal amplification technique to improve the sensitivity and accuracy of the lateral flow test strip biosensor. In addition, based on the Pt / Ti3C2T x nanoparticle as a signal carrier, a probe is prepared by labeling SH-DNA through Pt-S bonds, which can be applied to the detection of HIV-DNA, and can obtain detection results earlier, which is beneficial to the treatment of patients and the effective control of the virus.
Owner:HUBEI UNIV +1

Antenna signal amplification host installed in multiple modes

The utility model relates to a signal amplification host, belongs to the technical field of antenna signal amplification, and particularly relates to a multimode-mounted antenna signal amplification host, which comprises a host shell, a placement assembly, an equipment frame and bolts, and a ceiling assembly comprises a groove, a fixed plate, a sliding chute, a sliding rod, a rotating rod, a fixed rod, a sliding sleeve and a fixed block; the surfaces of the multiple sliding rods are fixedly connected with clamping plates, and the inner walls of the grooves are fixedly connected with clamping hoops. According to the utility model, through the arrangement of two installation modes of the placing assembly and the ceiling assembly, the adaptability of host installation can be improved, the host can be rapidly disassembled through the two installation modes, the later maintenance is more convenient, and the problems that most of the existing signal amplifier antennas are fixed on a table top or an equipment frame through bolts, and the cost is low are solved. The problems of single mounting mode, low mounting adaptability and inconvenience in replacement and maintenance of the antenna due to bolt fixation in the prior art are solved.
Owner:ENPING BAIKAL AUDIO EQUIP CO LTD

Detection method and application of OTA by a ratiometric fluorescence sensor based on CHA

The present invention provides a detection method and application of a ratio-type fluorescence sensor for detecting OTA based on CHA. A ratio-type fluorescence sensor is constructed by using CDs and FAM as dual-emission fluorescence probes in combination with the CHA signal amplification technology. Finally, the ratio F FAM / F CDs is used to achieve the quantitative detection of ochratoxin A, with a linear range of 5.0 pg / mL - 3.0 ng / mL and a detection limit of 1.5 pg / mL. The ratio-type fluorescence sensor for detecting ochratoxin A in the present invention not only has a self-calibration function, can eliminate the interference of various variable or difficult-to-quantify factors such as probe concentration, polarity, temperature, and excitation intensity, broaden the response range, and effectively improve the accuracy and reliability of the detection results, but also does not involve enzymes such as proteases in the whole operation process, is less affected by the external environment, has high sensitivity, low requirements for operating conditions and operating costs, can achieve the accurate detection of ultra-trace OTA, and has the value of industrial application and popularization.
Owner:HENAN AGRICULTURAL UNIVERSITY

Signal amplifier of pressure-resistant hydrophone

The invention relates to the technical field of signal amplification, in particular to a signal amplifier of a pressure-resistant hydrophone, and the signal amplifier comprises a shell which comprises an outer shell and an inner shell, the outer shell is of an annular hollow structure, the outer shell and the inner shell are fixedly connected through a pressure-resistant mechanism, and the pressure-resistant mechanism is annularly arranged between the outer shell and the inner shell; a cushioning mechanism is further arranged between the outer shell and the inner shell, the cushioning mechanism is annularly arranged between the outer shell and the inner shell, the sealing mechanism is located in the inner shell and the outer shell, the amplifying mechanism is located in the inner shell, and the amplifying mechanism and the inner shell are connected in a matched mode through the sealing mechanism; the stability of the structure under the dynamic load is enhanced.
Owner:LANGFANG KETAIDA TECH CO LTD

Detection chip, equipment and method for detecting HIV RNA (Human Immunodeficiency Virus Ribonucleic Acid) in saliva

The invention provides a detection chip, device and method for detecting HIV RNA in saliva, the detection chip comprises a chip substrate, a plurality of electric signal transmission interfaces are integrated on the chip substrate, the detection chip further comprises a reaction electrode, a differential electrode and a reference electrode, the surface of the reaction electrode is modified with nucleic acid aptamer molecules, and the surface of the differential electrode is modified with nucleic acid aptamer molecules. The detection module is used for generating a detection electric signal according to specific binding of nucleic acid aptamer molecules and HIV RNA; the differential electrode is a blank electrode and is used for deducting background signals generated by non-specific molecules in an added sample solution and a to-be-detected solution and amplifying a detection electric signal through the differential amplification circuit; the reference electrode is used for providing stable reference potential; the reaction electrode, the differential electrode and the reference electrode are electrically connected with an electric signal transmission interface respectively. According to the invention, the HIV RNA in the saliva is detected based on field effect transistor biological detection and by adopting a differential signal amplification technology, so that the effects of accurately detecting trace HIV RNA in the saliva, improving the yin-yang coincidence rate and simplifying the detection operation are achieved.
Owner:SUZHOU MORNINGSIDE NANO TECHNOLOGIES LTD

Specific probe for branched chain DNA amplification system and application thereof

The invention relates to a specific probe for a branched DNA amplification system and application of the specific probe, and belongs to the technical field of pathological diagnosis. On the basis of an original target gene probe, a pre-amplification probe, an amplification probe and a marking probe of a branched chain DNA amplification system probe system, a closing probe for closing the pre-amplification probe is added, and the closing probe can be complementarily combined with the pre-amplification probe. Probes used in the branched DNA signal amplification (bDNA) technology are all single-stranded DNA (ssDNA), and the combination of a closed probe and a pre-amplification probe can reduce the combination of non-target fragments in a sample and the ssDNA, so that the non-specific hybridization is reduced, and the specificity of the bDNA is improved.
Owner:HENAN CELNOVTE BIOTECHNOLOGY CO LTD

Smeglutide electrochemiluminescence immunoassay method and kit based on TSA signal amplification technology

The invention relates to a semeglutide electrochemiluminescence immunoassay method based on a TSA signal amplification technology and a kit, and solves the technical problem of how to detect semeglutide with simple and rapid operation, wide detection range and high detection sensitivity. The method is simple to operate, can quickly detect the semeglutide, and is wide in detection range and high in detection sensitivity.
Owner:WENZHOU KANGRUI BAIOU BIOTECHNOLOGY CO LTD

Hall signal amplification circuit and Hall sensing device

The invention discloses a Hall signal amplification circuit and a Hall sensing device, relates to the technical field of Hall signal amplification, and aims to solve the problem that a Hall sensing voltage amplification circuit in the prior art cannot completely eliminate offset voltage of an operational amplifier. Comprising a reference module, an offset calibration module and an integral amplification module, and the reference module can clamp the direct current level of the first output end of the Hall sensor at 1 / 2 power supply voltage; the offset calibration module can compensate the offset voltage of the integral operation amplification module and the offset voltage of the Hall sensor; the integral amplification module can amplify the compensated Hall sensing signal and output a final Hall sensing signal; the Hall sensing device comprises a Hall signal amplifying circuit; the Hall signal amplification circuit and the Hall sensing device can eliminate the imbalance of Hall signals.
Owner:HANGZHOU RUIMENG TECH

A mass spectrometry detection method based on single-base sequence mass label and its application

The present invention discloses a mass spectrometry detection method and application based on a single-base sequence mass label, which utilizes a single-base sequence DNA strand as a mass label for mass spectrometry immunoassay. After the nucleic acid sequence is labeled on the target, the DNA nucleic acid strand is enzymatically hydrolyzed into a single base by exonuclease and alkaline phosphatase, and the concentration of the base after enzymatic hydrolysis is detected by a liquid chromatography-mass spectrometry method to achieve quantification of the target. Compared with the prior art, the mass spectrometry immunoassay signal amplification technology of the present invention is simple, and the higher labeling efficiency makes the method have higher sensitivity, and can be widely used in the fields of biomarker detection and hazardous substance screening. The detection limit of the method of the present invention is much lower than the limit specified in the national standard, and can be used for the detection of low-concentration targets, providing a new method path for the screening of hazardous substances.
Owner:NANJING PRODUCT QUALITY SUPERVISION & INSPECTION INSTITUTE (NANJING QUALITY DEVELOPMENT & ADVANCED TECHNOLOGY APPLICATION RESEARCH INSTITUTE)

Immunohistochemical detection kit and method for PD-L1

The invention relates to the technical field of tumor diagnosis and immunohistochemical detection, and discloses a PD-L1 immunohistochemical detection kit and method.The kit is composed of a double-epitope high-affinity antibody, a color developing reagent, a signal amplification system, antigen repair liquid, a sealing reagent and the like. According to the detection method, a double-epitope antibody is combined with different active epitopes of PD-L1 in a targeted manner, the color development effect of a weak signal area is enhanced through a Tyramide dynamic signal amplification technology, meanwhile, the spatial distribution characteristics of PD-L1 and other immune markers are analyzed by introducing a multi-target fluorescence labeling technology, a multi-dimensional scoring model is established, and the tumor immune microenvironment is comprehensively evaluated. According to the method, the sensitivity and specificity of PD-L1 detection are improved, background noise interference is reduced, the problems of leak detection of a weak positive region, cross interference of multi-target detection, inaccurate single scoring model and the like are solved, and the method is widely applied to the fields of tumor immunotherapy adjoint diagnosis and microenvironment research.
Owner:JINAN UNIVERSITY

Proximity induced amplification system for efficiently visualizing sialic acid modified RNA (Ribonucleic Acid) and application of proximity induced amplification system

The invention relates to a proximity induction amplification system for efficiently visualizing sialic acid modified RNA and application of the proximity induction amplification system. The proximity induction amplification system comprises a molecular recognition module, a regulation and control module and a signal enhancement module, the molecular recognition module comprises an RNA recognition probe and a sialic acid recognition probe; the regulation and control module comprises a locking chain and an unlocking chain; and the amplification system comprises a probe H1 and a probe H2. According to the invention, through metabolic labeling and orthogonal chemical reaction and in combination with an HCR signal amplification technology, efficient and sensitive detection of sialylated RNA on a cell membrane is realized. The method has the advantages of simplicity and convenience in operation, good signal amplification effect, wide application range and the like, and can be widely applied to biomedical research and clinical diagnosis.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA

Wavelet decomposition-based multi-layer adaptive ripple recognition method and related equipment

The invention relates to the technical field of electrical detection, in particular to a multilayer adaptive ripple recognition method based on wavelet decomposition and related equipment. The method comprises the following steps: acquiring a high-voltage signal; the obtained high-voltage signal is preprocessed; performing signal amplification and digital processing on the preprocessed high-voltage signal through a signal amplification technology and an analog-to-digital conversion technology to obtain a preliminary digital signal; processing the preliminary digital signal through a wavelet decomposition algorithm to obtain direct current ripple data; performing feature extraction on the direct current ripple data through a wavelet basis to obtain an insulation state feature; and comparing the insulation state characteristics with a self-adaptive threshold value, and starting an early warning operation according to a comparison result. According to the method, the number of layers of wavelet decomposition and an analysis frequency band can be adaptively selected, energy evaluation and frequency domain positioning are carried out on decomposed detail components of each layer, effective layers containing ripple characteristics are dynamically identified, and online detection, alarm and data uploading are realized.
Owner:GUANGZHOU GRG METROLOGY & TEST CO LTD +1

Preparation method of fluorescence in situ hybridization kit based on hybridization chain reaction (HCR)

The invention belongs to the technical field of molecular biology, and discloses a preparation method of a fluorescence in situ hybridization kit based on a hybridization chain reaction (HCR), the core components of the kit comprise a hybridization probe, an amplification probe, a washing liquid, a confining liquid, protease K, HRP-mouse anti-digoxin, and a TSA developing liquid 520 nm. According to the FISH detection system combined with the HCR signal amplification technology, when a hybridization probe and an amplification probe are simultaneously combined to a position adjacent to a target sequence, a small section of sequence at the top end can trigger a branched HCR reaction to form a macromolecular nucleic acid aggregate. Signal amplification of HCR is achieved through cascade hybridization reaction, detection sensitivity and specificity can be remarkably improved, and meanwhile the problem of background noise caused by a traditional enzymatic method is avoided. The kit disclosed by the invention is wide in sample application range, and can be used for positioning analysis of target RNA of samples such as paraformaldehyde or formalin-fixed animal and plant tissues, paraffin or frozen sections, cells and the like, and high-sensitivity detection of the single cell level of non-coding RNA.
Owner:HANGZHOU BORRICK BIOTECHNOLOGY CO LTD

Method for detecting double miRNA in urine based on multiple signal amplification and metal ion selective recognition phenomenon and application thereof

The application provides a urine double-miRNA detection method based on multiple signal amplification and metal ion selective recognition phenomenon and application thereof, and relates to the technical field of biomedical diagnosis. 2+ and Ag + , which are respectively reacted with CDs and CdTe QDs, and finally, the fluorescence signals of CDs and the fluorescence signals of CdTe QDs are used for quantifying miRNA-375 and miRNA-148a. The application establishes a simple, rapid, sensitive and simultaneous homogeneous urine double-miRNA quantitative analysis strategy from the aspects of analysis instrument, analysis steps and cost, selection of signal reporting molecules, multiple nucleic acid signal amplification technology and application of selective recognition phenomenon.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV

Bimetallic oxides and preparation methods thereof and application of signal reversal detection of antibiotics

The present invention provides a bimetallic oxide and a preparation method thereof and an application of signal flipping detection of antibiotics. The bimetallic oxide is composed of Sc 2 O 3 Octahedral crystal structure of Sc and CuO 2 O 3 @CuO. The present invention also provides a bimetallic oxide composite material, mainly composed of Sc 2 O 3 @CuO and adsorbed on Sc 2 O 3 Composite material Sc composed of nano-gold particles on the surface of @CuO octahedron 2 O 3 @CuO@AuNPs. The present invention also provides a photocurrent signal reversal type photoelectrochemical sensor, comprising TiO 2 / ITO electrode and modified TiO 2 The hairpin probe HP2 on the surface of the ITO electrode, the auxiliary probe S2 labeled with biotin and the signal probe, the signal probe is Sc coated with streptavidin 2 O 3 @CuO@AuNPs, probe S2 opens probe HP2, allowing the signal probe to be modified to TiO 2 / ITO electrode surface, forming photoelectric signal and TiO 2 Sc / ITO electrode opposite 2 O 3 @CuO@AuNPs / TiO 2 / ITO electrode. In this way, the sensor is constructed by combining the photocurrent direction reversal strategy and enzyme cleavage signal amplification technology, which can eliminate the interference of background signals and increase signal output, thereby improving the selectivity and sensitivity of detecting antibiotics.
Owner:ZHENGZHOU UNIV

Low-permeability reservoir fractured well rapid well test analysis method based on small signal amplification technology

InactiveCN120578837AComplex mathematical operationsFinite conductivityWell test
The invention discloses a low-permeability reservoir fractured well rapid well test analysis method based on a small signal amplification technology. The method comprises the steps that an early analytical solution of a low-permeability reservoir fractured well trilinear flow model is obtained; obtaining an analytical solution and an early analytical solution of the infinite flow guide fracture model of the fractured well of the low-permeability reservoir; constructing a bottom hole pressure influence function of the fractured well of the low-permeability reservoir; constructing an analytical solution of a finite flow guide fracture model of the fractured well of the low-permeability reservoir, and calculating the bottom-hole theoretical pressure of the fractured well of the low-permeability reservoir; obtaining an early analytical solution of the low-permeability reservoir finite flow guide fracture model, and further constructing a pressure-pressure derivative difference function based on a small signal amplification technology; and developing a new double logarithmic curve chart for rapid well testing of the fractured well of the low-permeability reservoir, explaining pressure measurement data of the fractured well of the low-permeability reservoir, and obtaining reservoir parameters. The method provided by the invention is simple and easy to understand, simple to operate, rapid in calculation, capable of effectively reducing the multiplicity of well test interpretation of the pressure measurement data of the low-permeability reservoir fractured well, and wide in application prospect.
Owner:SOUTHWEST PETROLEUM UNIV

A multi quadrotor aircraft position consensus control system based on attack isolation and privacy protection

The application discloses a position consistent control system of multi-four-rotor aircraft based on attack isolation and privacy protection and belongs to the technical field of industrial process control; the position consistent control system comprises a Liu password system, a network attack detection and isolation system and a consistent control system; the Liu password system performs encrypted transmission on provided leader expected signals and position information and obtains decrypted information; the network attack detection and isolation system detects attacked followers and isolates the attacked followers; input ends of the consistent control system are connected with output ends of an undirected graph G; and output ends of the consistent control system are connected with input ends of the i-th follower. The signal amplification technology of the Liu password system is used to protect the sensitivity of information and reduce errors between plaintext encryption and decryption; the consistent controller is used to compensate unknown dynamics and errors between real signals and decrypted signals through a small number of learning parameters, so that the protection of sensitive information and network security of the multi-four-rotor aircraft during task execution are ensured.
Owner:NANJING UNIV OF POSTS & TELECOMM

CRISPR (clustered regularly interspaced short palindromic repeats) light-operated one-tube detection kit based on G4-crRNA / AZD < + + > and detection method

The invention belongs to the field of biological nucleic acid detection, and discloses a G4-crRNA / AZD < + + >-based CRISPR (clustered regularly interspaced short palindromic repeats) light-controlled tubular detection kit, which comprises the following components: G4-crRNA / AZD < + + >, Cas12a protease, an RPA (recombinase polymerase) isothermal amplification reagent, a buffer solution and a fluorescence report probe. The kit can obviously improve the detection sensitivity of a target, is high in specificity, effectively shortens the detection time, and is high in universality. The invention further discloses a corresponding detection method, the activity of the CRISPR system is started in a light-operated mode by combining a nucleic acid amplification signal amplification technology, the problem that the nucleic acid amplification efficiency is reduced due to cis-cleavage and trans-cleavage of Cas protease is avoided, rapid detection of targets in various samples can be achieved, and the detection accuracy is improved. A basis is provided for establishing a detection method which is low in cost, high in simplicity, strong in universality, high in sensitivity and good in specificity.
Owner:HUNAN PROVINCIAL TUMOR HOSPITAL

High-performance multi-channel arc channel type electron multiplier and preparation method thereof

PendingCN121885503AMultiplier electrode arrangementsVessels or leading-in conductors manufactureLow noiseChannel electron multiplier
The invention relates to the technical field of electronic detection and signal amplification, in particular to a high-performance multi-channel arc channel type electron multiplier, which comprises an electronic input end used for receiving and focusing incident particles; the partition area is used for establishing a potential gradient; the alloy coating electrodes are respectively arranged at the input end and the output end of the electron multiplier and are used for applying working voltage; the multi-channel arc-shaped array structure comprises a plurality of arc-shaped channels which take the center of the section of the electron multiplication area tube body as the circle center and are uniformly arranged on the same circumference, and the arc-shaped channels are parallel to one another; the voltage division area is used for stabilizing electric field distribution in the channel; compared with the prior art, the high-gain and high-resolution collaborative improvement is realized, the high-temperature-resistant stability is excellent, the working life is ultra-long, meanwhile, low noise, quick response and efficient signal detection are achieved, the performance reaches the import standard, and a reliable core component is provided for localization of high-end instruments.
Owner:SUZHOU JIJIKE INSTR CO LTD

Gene marker for evaluating risk of pulmonary arterial hypertension related to congenital heart disease and application of gene marker

The invention relates to the technical field of biomedical detection, and discloses a gene marker for evaluating the risk of congenital heart disease related pulmonary hypertension and application, the gene marker is S100A3, and the expression level of the gene marker can evaluate the risk of congenital heart disease related pulmonary hypertension. According to the present invention, the detection sensitivity and the clinical prediction value can be significantly improved by combining the specific antibody, the nanometer signal amplification technology and the multi-omics data analysis.
Owner:CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV

FM + DAB signal amplification circuit and electronic equipment thereof

The utility model provides an FM + DAB signal amplification circuit and electronic equipment thereof, and relates to the technical field of signal amplification, the circuit comprises a receiving port antenna input port, an amplifier circuit, a power divider circuit, an LC band elimination filter circuit and an output module, a receiving port is electrically connected with the input end of the amplifier circuit, and the output module is electrically connected with the input end of the power divider circuit. The output end of the amplifier circuit is connected with the input end of the power divider circuit, the output end of the power divider circuit is connected with the input end of the LC band-stop filter circuit, the output end of the LC band-stop filter circuit is connected with the output module, and the receiving port is configured to receive a DAB / FM signal. According to the amplifying circuit, an LCR is used for building a power dividing circuit, an LC filter circuit is used, and the signal amplifying requirement of two frequency bands of two receivers is met through one amplifying circuit.
Owner:YUANXI CHUANGYING AUTOMOTIVE TECHNOLOGY (SHANGHAI) CO LTD