DNA determining electrochemical sensor and method based on platinum nano particle catalysis electrochemistry circulation signal amplification technology

A cyclic signal, gold nanoparticle technology, applied in the field of analytical chemistry and electrochemical sensors, can solve the problems of complex methods and low sensitivity of the method, and achieve the effect of high sensitivity

Active Publication Date: 2014-08-27
GUANGZHOU JINGKE DX CO LTD
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  • Abstract
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Problems solved by technology

These methods have their own advantages and can meet the requirements of DNA detection to varying degrees, but the methods are either not sensitive or complicated

Method used

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  • DNA determining electrochemical sensor and method based on platinum nano particle catalysis electrochemistry circulation signal amplification technology
  • DNA determining electrochemical sensor and method based on platinum nano particle catalysis electrochemistry circulation signal amplification technology
  • DNA determining electrochemical sensor and method based on platinum nano particle catalysis electrochemistry circulation signal amplification technology

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example

[0030] Example: An electrochemical sensor for measuring DNA based on platinum nanoparticle catalyzed electrochemical cycle signal amplification technology and a method for measuring DNA.

[0031] 1. Experimental part

[0032] 1.1 Instruments and reagents

[0033] 1.1.1 Instruments and equipment

[0034] CHI660B electrochemical workstation, Shanghai Chenhua Instrument Company; Anke-TGL-16C Feiweng high-speed centrifuge, Shanghai Anting Scientific Instrument Factory; HS-3D acidity meter, Shanghai Leici Instrument Factory; three-electrode system for experiments: The gold electrode and the modified gold electrode are the working electrodes, the Ag / AgCl (saturated KCl) electrode is the reference electrode, and the platinum wire electrode is the counter electrode.

[0035] 1.1.2 Reagents

[0036] Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), chloroauric acid (HAuCl 4 ), chloroplatinic acid (H 2 PtCl 6 ), trisodium citrate (Na 3 C 6 h 5 o 7 ), alumina powder (α-A1 2 ...

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Abstract

The invention relates to a preparing method of a DNA determining electrochemical sensor based on a platinum nano particle catalysis electrochemistry circulation signal amplification technology and the application of the electrochemical sensor. A hairpin DNA 1 is assembled on the surface of a platinum nano particle modified gold electrode in a self-assembly mode, and when a target DNA 2 exists, due to the fact that the target DNA 2 and the hairpin DNA 1 assembled on the surface of the gold electrode are partially paired in a complementary mode, the hairpin structure of the DNA 1 is opened. Then, another hairpin DNA 3 chain replaces the target chain DNA 2 by competing pairing hybridization, the target chain DNA 2 is released, and the released target chain is continuously opened to form a new hairpin structure. The action is carried out in a cycling mode, recycling of the target chain DNA 2 is achieved, a prepared nano particle electrochemical probe is combined, signal amplification is achieved, the prepared electrochemical sensor is used for catalyzing cycling between PAP and PQI, further electrochemistry signal amplification is achieved, a cyclic voltammetry or DPV is used for determining current signal intensity, and according to the measured current signal intensity, determining on the target chain DNA2 is achieved. The sensor has high detecting sensitivity.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and electrochemical sensors, and relates to an electrochemical sensor for measuring DNA based on a platinum nanoparticle catalytic electrochemical cycle signal amplification technology and a method for measuring DNA. Background technique [0002] DNA is the genetic molecule in living organisms. The detection of DNA is usually related to the diagnosis of various diseases, so DNA detection has important research significance. At present, due to the continuous improvement of DNA detection technology, the detection of other biological molecules such as proteins and small molecular substances can also be converted into the detection of DNA. There are many methods for DNA detection, such as enhanced Naman scattering technology (Wei, X., Su, S., Guo, Y., Jiang, X., Zhong, Y., Su, Y., Fan, C., Lee, S.T. and He, Y. (2013) A Molecular Beacon‐Based Signal‐Off Surface‐Enhanced Raman Scattering Strategy f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/48
Inventor 混旭柏莉谢国亮
Owner GUANGZHOU JINGKE DX CO LTD
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