Kit for chemiluminiscence immunoassay as well as preparation method and application of kit

Abstract

The invention discloses a kit for chemiluminiscence immunoassay as well as a preparation method and application of the kit. The kit comprises an immunomagnetic bead compound coated with an antigen oran antibody of an object to be detected, and a secondary antibody or a second antibody coupled with a polymer molecule, and a plurality of light source groups are marked on polymer molecules. Due to the adoption of a signal amplification technology, a plurality of luminescent groups are covalently coupled to polymer molecules, and then the polymer molecules are covalently coupled to a secondary antibody or a second antibody, so that the luminescent groups are multiplied, the luminous intensity is multiplied, and the kit can be used for detecting biological indexes with extremely low content.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to a kit for chemiluminescent immunoassay and its preparation method and application. Background technique [0002] Chemiluminescence immunoassay includes two parts: immunoassay and chemiluminescence analysis. Immunoassay uses chemiluminescent substances or enzymes as markers, directly marks on antigens or antibodies, and forms antigen-antibody immune complexes through the reaction of antigens and antibodies. Chemiluminescent analysis is to add an oxidant or enzyme luminescent substrate after the immune reaction is over. After the chemiluminescent substance is oxidized by the oxidant, an intermediate in an excited state will be formed, which will emit photons and release energy to return to a stable ground state and emit light. Intensity can be detected using a luminescent signal measuring instrument. According to the relationship between the chemiluminescence marker and the luminescence...

AI Technical Summary

Problems solved by technology

However, this technique requires a certain amount of samples, and it cannot accurately detect certain ultra-low levels of disease markers or drug concentrations.

At present, some chemiluminescent detection methods, such as chemiluminescent enzyme immunoassay, are applied to signal amplification technology, which can amplify the luminescent enzyme signal, but there are also defects, such as repeated annealing and enzyme addition, and, for direct chemiluminescent As far as marker immunoassays are concerned, it is also not suitable to enhance the detection effect by amplifying the luminescent enzyme signal. Therefore, direct chemiluminescent marker immunoassays cannot be applied to biological assays with extremely low levels of analytes.

[0004] At present, the commonly used chemiluminescence system is direct chemiluminescence labeled with acridinium ester or luminol-based luminescent groups, but the sensitivity of such methods often does not meet the clinical requirements, which limits the promotion of this method

Images