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Electrochemical method for detecting microcystic toxins

A microcystin, electrochemical technology, applied in the field of analytical chemistry or water environment monitoring, can solve the problems of inability to identify toxins, false positive reactions, etc., and achieves low-cost instruments and equipment, simple and easy methods, and excellent electrical conductivity. Effect

Inactive Publication Date: 2017-08-18
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunoassay is simple, fast, and highly sensitive, but it cannot distinguish toxins well, and false positive reactions sometimes occur

Method used

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  • Electrochemical method for detecting microcystic toxins
  • Electrochemical method for detecting microcystic toxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Flake MoS 2 Preparation of nanomaterials:

[0035] Preparation of MoS by hydrothermal method 2 , weighed 90 mg of sodium molybdate and 180 mg of thioacetamide in 60 mL of distilled water, added 0.5 mL of 1 M NaOH to adjust the solution to be alkaline, transferred to a 100 mL reaction kettle, and reacted hydrothermally at 220 °C for 48 h, cooled to room temperature, washed with deionized water and ethanol several times, the precipitate was collected by centrifugation, and dried in vacuum at 80 °C for 12 h to obtain MoS 2 Nanomaterials, stored for later use.

Embodiment 2

[0037] Preparation of AuNCs:

[0038] The bovine serum albumin-mediated method was used to synthesize AuNCs. The synthesis process was mild and non-toxic, and the quantum yield of the product was greatly improved. The glass instruments required for the synthesis were first soaked in aqua regia, washed with deionized water, and dried for later use. 10 mL of 5 mM HAuCl at 37°C 4 Mix with 5 mL of 50 mg / mL bovine serum albumin (BSA), stir for 5 minutes, BSA interacts with chloroauric acid ions, coat the chloroauric acid ions inside the protein, and then add 0.5 mL of 1 M NaOH to adjust the solution to be Basic, enhances the reducing ability of amino acid residues in BSA. The reaction was stirred at 37°C for 12 h, and the chloroauric acid was reduced to obtain a red fluorescent AuNCs solution, which was stored for future use.

example 3

[0040] Preparation of Au@Pt core-shell nanomaterials:

[0041] Au@Pt core-shell nanomaterials were prepared by seed growth method. First, a seed solution of gold nanoparticles (AuNPs) with a particle size of 19 nm was prepared by the sodium citrate reduction method. Afterwards, a certain amount of AuNPs seed solution was mixed with 10 mL of 1 mM H 2 PtCl 6 The solution was stirred in a 100 mL round bottom flask. In a constant temperature oil bath at 80 °C, 5 mL of 10 mM ascorbic acid solution was slowly added dropwise (10 drops / min), and refluxed at a constant temperature of 80 °C for 30 min to obtain Au@Pt core-shell nanomaterials.

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Abstract

The invention relates to an electrochemical method for detecting microcystic toxins based on a nano material and Au@Pt marked antibody signal amplification technology and belongs to the technical field of analytical chemistry or water environment monitoring. The electrochemical method comprises the following steps: fixing an MoS2 / AuNCs nano composite to the surface of a gold electrode through an Au-S bond, and then modifying an MC-LR antibody to the surface of the nano composite by utilizing the adsorption action; specifically combining the MC-LR antibody and an antigen, and then by combining an antibody marked by an Au@Pt core-shell material, realizing amplification of an electrochemical response signal by catalyzing H2O2; and realizing trace determination of MC-LR in a water sample according to the amplified electrochemical signal. The electrochemical method has the characteristics of high sensitivity, high specificity, simpleness, rapidness and the like. A detection method provided by the invention can also be used for detecting molecules and small molecules of algae toxins of other species in water and provides a rapid and convenient detection way for pollutants in the water.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry or water environment monitoring, and in particular relates to an electrochemical method for detecting microcystins in water samples based on nanometer material signal amplification technology. Background technique [0002] Microcystins (MCs for short) are freshwater cyanobacterial hepatotoxins that can cause animal poisoning and affect human health. Leucine-arginine) (Microcystin-(leucine-arginine), MC-LR). With the increasing eutrophication of water bodies, microcystin pollution caused by cyanobacterial blooms has become a global environmental and health concern. The content of MC-LR in water has been used by many countries and organizations as an important indicator of water quality standards. The World Health Organization (WHO) first publicly expressed the need to monitor cyanobacterial toxins in water in 1998, and subsequently recommended cyanotoxins in drinking water. The micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/32
CPCG01N27/30G01N27/301G01N27/32
Inventor 庞鹏飞陈梦腾霞张艳丽王红斌
Owner YUNNAN MINZU UNIV
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