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A method for the detection of acetylcholine based on surface-enhanced Raman spectroscopy

A surface-enhanced Raman and acetylcholine technology, applied in the fields of biophotonics and nanophotonics, can solve the problems of high cost, complicated operation, insufficient sensitivity, etc., and achieve the effects of short time-consuming, low-cost detection, and simple operation.

Active Publication Date: 2020-02-18
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking fluorescence detection as an example, it usually requires multiple enzyme-catalyzed reactions to gradually convert acetylcholine into substances sensitive to certain fluorescent molecules before performing fluorescence detection. The operation process is lengthy, costly, and conversion efficiency is limited; However, electrochemical detection usually has disadvantages such as high cost, complicated operation, long time consumption, and insufficient sensitivity.
Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive, fast, and low-cost spectral detection method, but the SERS activity of acetylcholine itself is low, and the sensitivity of directly using surface-enhanced Raman scattering to detect acetylcholine is difficult to cope with the physiological environment. Trace detection of acetylcholine

Method used

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  • A method for the detection of acetylcholine based on surface-enhanced Raman spectroscopy
  • A method for the detection of acetylcholine based on surface-enhanced Raman spectroscopy
  • A method for the detection of acetylcholine based on surface-enhanced Raman spectroscopy

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Experimental program
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Effect test

Embodiment 1

[0032] The piranha solution involved in this embodiment is prepared by mixing concentrated sulfuric acid with a mass fraction of 98% and hydrogen peroxide with a mass fraction of 30% in a volume ratio of 3:1.

[0033] 1. Preparation of silver colloidal solution: Dissolve 11 mg of silver nitrate in 61.25 ml of deionized water, heat and stir until boiling, then add 1.25 ml of 1% by weight trisodium citrate dihydrate solution, continue the reaction for 50 minutes, then stop heating, Obtain silver colloidal solution after cooling, stand-by;

[0034] 2. Preparation of surface-enhanced Raman spectroscopy (SERS) substrate: soak and clean the glass sheet in piranha solution, rinse the remaining piranha solution on the glass sheet with deionized water, and dry it in an oven at 100°C for two hours; Dry glass sheet and clean polydimethylsiloxane (PDMS) chip are bonded to obtain microfluidic chip; 1wt% polydiallyldimethylammonium chloride (PDDA) aqueous solution is injected into microflui...

Embodiment 2

[0037] The piranha solution involved in this embodiment is prepared by mixing concentrated sulfuric acid with a mass fraction of 98% and hydrogen peroxide with a mass fraction of 30% in a volume ratio of 3:1.

[0038] 1. Preparation of gold-core silver-shell nanorods

[0039] 1) To 2.5ml, 0.2M cetyltrimethylammonium bromide (CTAB) solution, add 1.5ml, 1mM chloroauric acid solution and 600μL, 0.01M sodium borohydride solution respectively, and stir for 2 minutes to form The seed solution is ready for use;

[0040] 2) Add 250μL, 15mM silver nitrate solution and 1.25ml, 15mM chloroauric acid solution to 2.5ml 0.2M CTAB solution in sequence, then add 0.08M ascorbic acid until the solution is colorless, then add 125μL of seed solution to form gold nanoparticles stick ready for use;

[0041] 3) Mix 2ml gold nanorod solution and 0.0728g CTAB, then add 4ml deionized water, 260μL, 0.1M ascorbic acid solution, 200ul, 15mM silver nitrate solution, 480μL, 0.1M sodium hydroxide solution ...

Embodiment 3

[0046] The piranha solution involved in this embodiment is prepared by mixing concentrated sulfuric acid with a mass fraction of 98% and hydrogen peroxide with a mass fraction of 30% in a volume ratio of 3:1.

[0047] 1. Preparation of gold colloidal solution: add 100ul of 10% chloroauric acid solution into 100ml of deionized water, heat and stir until boiling, then add 4ml of 1% by weight trisodium citrate dihydrate solution, and continue the reaction for 50 minutes Stop heating, obtain gold colloidal solution after natural cooling, stand-by;

[0048] 2. Preparation of surface-enhanced Raman spectroscopy (SERS) substrate: soak and clean the glass sheet in piranha solution, rinse the remaining piranha solution on the glass sheet with deionized water, and dry it in an oven at 100°C for two hours; A dry glass sheet is bonded to a clean polydimethylsiloxane (PDMS) chip to obtain a microfluidic chip; 0.02wt% aqueous solution of 3-aminopropyltriethoxysilane is injected into the mic...

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Abstract

The invention discloses a surface enhance Raman scattering based acetylcholine detection method. According to the method, the competition between acetylcholine and organic molecules on a metal nano substrate is utilized, under the assistance of SERS, corresponding relationship between surface enhanced Raman strength of organic molecules and acetylcholine concentration is established, and highly sensitive, low cost, simple, and rapid acetylcholine detection is realized.

Description

technical field [0001] The invention relates to a method for detecting acetylcholine based on surface-enhanced Raman spectroscopy, and belongs to the technical fields of biophotonics and nanophotonics. Background technique [0002] Neurotransmitter is an important substance for transmitting information between neurons, and it is also an important way for the central nervous system to control and regulate human physiological functions. An imbalance of any neurotransmitter can cause a series of related diseases, such as Parkinson's, depression, Alzheimer's disease, etc. Therefore, accurate and effective detection of neurotransmitter concentration has far-reaching significance for the clinical diagnosis and treatment of related diseases and the study of the mechanism of neurotransmitters. [0003] Acetylcholine is a very typical neurotransmitter in the human body, and it is also one of the important neurotransmitters in the central cholinergic system. The concentration of ace...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 王著元李浪宗慎飞崔一平
Owner SOUTHEAST UNIV
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