46results about How to "Low cost detection" patented technology

The invention relates to a comprehensive ocular surface analyzer based on an expert system. The comprehensive ocular surface analyzer comprises a comprehensive ocular surface analyzer hardware module, an image processing module and an expert system module, wherein the comprehensive ocular surface analyzer hardware module comprises a controller, a structured illumination module and an imaging module; the image processing module comprises a tear meniscus height measuring module, a red eye detection module, a tear film stability detection module, a meibomian gland morphology detection module and a corneal topography analysis module; and the expert system takes information acquired though image processing as the input, performs objective and correct judgment on common diseases of the human ocular surface and provides therapeutic measures.

The invention discloses a method for detecting the toxic impurity DMAP in antibiotic drug raw materials. The antibiotic drug raw materials are dissolved, diluted and centrifuged to obtain supernate, the supernate is subjected to high performance liquid chromatography-quadrupole series linear ion trap mass spectrum, and then the content of the toxic impurity DMAP in the antibiotic drug raw materials is detected. The mobile phase of high performance liquid chromatography is a mixture of ammonium acetate aqueous solution and acetonitrile solution. The mass-to-charge ratio (m / z) of the parent ion in quadrupole series linear ion trap mass spectrum for detecting DMAP is 122.7, and the mass-to-charge ratio (m / z) of the daughter ion is 107.1. The adopted pair of ions does not interfere with sample detection and is highest in response. By the adoption of the mobile phase, the retention time of a target compound on a chromatographic column can be prolonged, so that a detection result is more stable. The method is easy to operate, and the result is reliable. Quick and low-cost detection of the residual quantity of DMAP in the antibiotic drug raw materials can be achieved, and detection sensitivity can reach 0.50 mg / kg.

The invention belongs to the field of microbiological detection, and particularly relates to a rolling circle constant-temperature amplification quick detection primer and a kit for mycoplasma pneumonia. The primer is designed according to a specific conservative target sequence of a mycoplasma pneumonia P1 gene; the primer consists of four primers, including a front end inner primer SIP, a rear end inner primer ASIP, and outer primers S and AS; in the pair of the outer primers S and AS, the S primer is positioned on the upstream of the SIP, and the AS primer is positioned on the downstream of the ASIP. The rolling circle constant-temperature amplification quick detection primer and the kit for the mycoplasma pneumonia can be used for quick detection of the mycoplasma pneumonia, provide guarantee for clinical early diagnosis and treatment, and realize an important value of quick, accurate, convenient and low-cost detection.

The invention belongs to the field of pesticide detection and provides a method capable of quickly detecting paraquat in beverages. The method capable of quickly detecting the paraquat in the beverages includes the steps of diluting samples of beverages such as fruit juice and vegetable juice, using gold nanoparticle sol as a substrate, and detecting the paraquat in the samples through surface-enhanced Roman spectroscopy. The method capable of quickly detecting the paraquat in the beverages has the advantages that the residual paraquat in the samples can be detected qualitatively and quantitatively quickly and effectively; the method is high in sensitivity, low in detection limit and capable of detecting the 0.1 microgram / mL paraquat effectively only within a few minutes, and can only detect the above 5 micrograms / mL paraquat in undiluted samples, so that the paraquat can be detected quickly, practically, efficiently and economically.

The invention discloses a method for detecting brain natriuretic peptide through a composite probe fluorescence "turning on-turning off-turning on" strategy on the basis of cobalt nanomaterial / nucleicacid aptamer. According to the method, a CoOOH nano-ring with a high-efficiency fluorescence quenching effect is synthesized; a CoOOH nano-ring / FAM-aptamer composite probe is constructed; backgroundsignals are reduced by means of the fluorescence quenching effect of the CoOOH nano-ring for a dye; the fluorescent dye can be made to move away from the CoOOH nano-ring by means of the recognition effect of the BNP (brain natriuretic peptide) for the specific molecules of FAM-aptamer, so that fluorescent signals can be recovered; and fluorescence recovery efficiency and BNP concentration are in agood linear relationship in a range of 0.030 to 0.80pg / mL. The method has the advantages of simplicity, rapidness, high sensitiveness, high specificity and the like, and can be used for the quantitative detection of BNP in clinical specimens. ROC curve analysis shows that the method has high accuracy in diagnosing heart failure (AUC=0.87, 95% CI: 0.71-1.00, and P<0.01), and the method has good specificity (diagnostic specificity is 87. 9%); and the use cost of the method is greatly reduced. The method is suitable for being widely promoted and used in the diagnosis and prognosis of the heart failure of children and adults in primary-level organizations.

The invention belongs to the technical field of physiological sample detection, and provides a physiological sample detection method which comprises the following steps: disposable chip boxes are respectively or simultaneously moved to corresponding set stations on the same detection platform along at least two linear paths, or along at least one linear path and at least one arc path, or along atleast two arc paths; and the physiological samples in the disposable chip box are correspondingly detected at the corresponding set stations, so that the integration and expansion of various detectionmethods are realized, the technical problems of high detection cost and huge detection equipment volume caused by the validity period of bottle opening are effectively solved, and high-efficiency andlow-cost detection is realized.

The invention relates to a thyroid dysfunction model and an establishment method thereof. The method comprises steps that (1), fresh blood from patients with the thyroid dysfunction and persons with the normal thyroid function are collected; serum is extracted to obtain serum samples; (2), the serum samples are detected through a Horiba confocal Raman spectrograph to obtain the serum Raman spectra; (3), fitting of all the serum autofluorescence background is performed, a baseline is corrected, and the corrected spectral data is subjected to integral area normalization processing to obtain thespectral data after processing; and (4), after the processed spectral data is subjected to dimensionality reduction and feature extraction through the PCA method, a SVM classification model is established, and the thyroid dysfunction model is obtained. The method is advantaged in the thyroid dysfunction model is established, based on the model, fast low-cost detection of thyroid diseases can be achieved.

The invention discloses a method for detecting starting material A in landiolol hydrochloride raw material drug, which comprises: a step of pre-treating landiolol hydrochloride raw material drug, and pre-treated landiolol hydrochloride raw material The drug is detected by liquid chromatography-tandem mass spectrometry, and the internal standard method is used to quantitatively analyze the starting material A in the landiprolol hydrochloride raw material drug. The detection sensitivity of the method of the invention can reach 0.50 mg / kg, and the precision and recovery rate of the method can both meet the detection requirements of (S)-(+)-m-nitrobenzene sulfonic acid glycidyl ester. The invention has the advantages of simple operation and reliable results, and can realize rapid and low-cost detection of trace (S)-(+)-m-nitrobenzenesulfonate glycidyl ester in the raw material drug of landipolol hydrochloride.

The present invention provides a paper-based detection method for exosomes, including: (1) recognition and molecular conversion system, (2) amplification reaction system, and (3) LFB detection and analysis system. The present invention converts exosomes into single-stranded DNA targets, amplifies the single-stranded DNA through nucleic acid amplification technology, and further utilizes PtAu-NiO 2 The amplified single-stranded DNA is detected by the lateral flow chromatography test paper with the nanozyme as the signal probe. The invention uses nucleic acid lateral flow chromatography test paper as the terminal detection platform, solves the problem that exosomes cannot be detected qualitatively and quantitatively at the same time, and realizes simple and low-cost detection of exosomes.

The invention belongs to the technical field of nano composite material preparation, and particularly relates to preparation of a core-shell type magnetic multi-color fluorescent probe and a visible detection method. The preparation of the rare-earth doped core-shell type magnetic multi-color fluorescent probe comprises the following steps: (1) loading fluorescein to magnetic halloysite by using a hydrothermal method, wherein the magnetic halloysite can show green fluorescence under a three-purpose ultraviolet analyzer; (2) to prevent the fluorescein from leaking out from the magnetic halloysite, dropping ammonium hydroxide into a mixed liquid of APTES+TEOS, and performing a reaction to substance hydroxyl of the product by amino so as to form a layer of a protection membrane; (3) grafting an ethylenediamine tetraacetic acid derivative, performing coordination with rare-earth ions to form a chelate compound, thereby obtaining the fluorescent probe. The invention further relates to a visible detection method. Fluorescent testing on DPA of detected substances of different concentrations is carried out after the fluorescent probe is mixed with a Tris-HCl buffer solution, and multi-color fluorescent variations show the recognition performance of the fluorescent probe.

The invention discloses a surface enhance Raman scattering based acetylcholine detection method. According to the method, the competition between acetylcholine and organic molecules on a metal nano substrate is utilized, under the assistance of SERS, corresponding relationship between surface enhanced Raman strength of organic molecules and acetylcholine concentration is established, and highly sensitive, low cost, simple, and rapid acetylcholine detection is realized.

The invention relates to a method for synchronously detecting biochemical oxygen demand and nitrate nitrogen content in a water body based on bidirectional electron transfer electrochemical active microorganisms and mainly aims to solve problems of detection hysteresis, complex operation, high cost and incapability of synchronous detection in the water body water quality biochemical oxygen demandand nitrate nitrogen detection process in the prior art. The method is advantaged in that a bioelectrochemical system taking electrochemical active microorganisms with bidirectional electron transfercapability as a core is constructed, step potentials are set to measure the electricity generation capacity and the electrophilic capacity of the system respectively, and rapid real-time detection ofbiochemical oxygen demand and nitrate nitrogen in the water body can be realized by utilizing an output current signal during electricity generation of the system, an input current signal during electrophilic and a standard curve model of biochemical oxygen demand and nitrate nitrogen.