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Method for detecting DNA by electrochemical transducer with signal amplification technology

An electrochemical and sensor technology, applied in the field of analytical chemistry and electrochemistry, can solve the problem of low sensitivity

Active Publication Date: 2014-09-03
广州方维信息科技有限公司
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  • Abstract
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Problems solved by technology

These methods have their own advantages and can meet the detection requirements of DNA to varying degrees, but the sensitivity is not high

Method used

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  • Method for detecting DNA by electrochemical transducer with signal amplification technology
  • Method for detecting DNA by electrochemical transducer with signal amplification technology
  • Method for detecting DNA by electrochemical transducer with signal amplification technology

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[0035] Example: Signal Amplification Technology Electrochemical Sensor Detection Method for DNA

[0036] 1. Experimental part

[0037] 1.1 Instruments and reagents

[0038] CHI660B electrochemical workstation (Shanghai Chenhua Instrument Co.); Anke-TGL-16C Feiweng high-speed centrifuge (Shanghai Anting Scientific Instrument Factory); PHS-3D acidity meter (Shanghai Leici Instrument Factory); using three electrodes System: gold electrode and modified gold electrode are working electrodes, Ag / AgC1 (saturated KCl) electrode is reference electrode, and platinum wire electrode is counter electrode.

[0039] Na 2 HPO 4 12H 2 O, Tianjin Hongyan Chemical Reagent Factory; Tris (2-carboxyethyl) phosphine hydrochloride (TCEP, tris (2-carboxyethyl) phosphine hydrochloride), chloroauric acid (HAuCl 4 ), trisodium citrate (Na 3 C 6 h 5 o 7 ) were purchased from Tianjin Bodi Chemical Co., Ltd.; potassium ferricyanide: K 3 [Fe(CN) 6 ], Tianjin Bodi Chemical Co., Ltd.; Potassium ferr...

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Abstract

The invention relates to a preparation method and application of an electrochemical transducer with a signal amplification technology. The preparation method comprises the following steps: firstly, preparing a gold-platinum nano particle, fixing thionine as an electrochemical reagent on the surface of the gold-platinum nano particle, fixing probe DNA on the surface of the gold-platinum nano particle so as to obtain an electrochemical probe, and simultaneously, self-assembling a hairpin DNA on the surface of a gold electrode to obtain an electrochemical transducer. Target DNA and the hairpin DNA assembled on the surface of the gold electrode are partially complemented and paired so as to open a hairpin structure when the target DNA is added to the electrochemical transducer, then another hairpin DNA chain replaces a target DNA chain through a competitive effect, and thus the target DNA chain is released; the released target chain unceasingly opens other hairpin structured DNA of the surface of an electrode, thereby realizing the recycling of the target DNA chain under the cyclic action, and the recycling action is combined the prepared electrochemical probe to measure target DNA. The transducer provided by the invention is high in selectivity and detection sensitivity, the gold-platinum nano particle is utilized as a carrier to support thionine to form the probe, and the transducer has the advantage of electrochemical stability.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and electrochemistry, and relates to a preparation method and application of an electrochemical sensor with signal amplification technology. In addition, the present invention also relates to a method for detecting DNA using the electrochemical sensor. Background technique [0002] Detection of specific sequence DNA is very important in clinical diagnosis, gene therapy and other biomedical research. Therefore, it is necessary to establish a method for rapid detection of DNA with high sensitivity. [0003] In general, DNA detection is based on DNA hybridization. In order to read out the hybridization reaction, the usual method is to label the partially complementary sequence of the target DNA. Generally, fluorescent, electrochemiluminescent, chemiluminescent substances, etc. are used to mark the partial complementary sequence of the target DNA to prepare DNA probes, and the concentr...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 混旭柏莉刘芳徐长旺
Owner 广州方维信息科技有限公司
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