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Method for detecting metal ions based on hybrid chain type reaction signal amplification technique

A hybrid chain reaction and signal amplification technology, applied in the field of analytical chemistry or environmental monitoring, can solve problems such as threats to aquatic organisms and human health, achieve high selectivity and anti-interference ability, increase specificity, and enhance selectivity

Inactive Publication Date: 2017-11-28
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there must be some silver-containing residues discharged into the water environment, which poses a potential threat to aquatic organisms and human health.

Method used

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  • Method for detecting metal ions based on hybrid chain type reaction signal amplification technique
  • Method for detecting metal ions based on hybrid chain type reaction signal amplification technique

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Detection of heavy metal Ag based on the combination of magnetic nanoparticles and hybrid chain reaction amplification technology+ ionic method

[0034] The invention is based on the combined detection of heavy metal Ag based on magnetic nanoparticles and hybrid chain reaction amplification technology + The principle of the ion method is as follows figure 1 shown. C-base-rich nucleic acid strand S1 is fixed to Fe via Au-S bond 3 O 4 @Au core-shell magnetic nanoparticles surface, on the target silver ion Ag + In the presence of ferrocene, another C-base-rich nucleic acid strand S2 labeled with ferrocene passes through C-Ag + The -C structure forms double-stranded DNA with S1 on the surface of the magnetic particle. When the ferrocene-labeled hairpin DNA H1 and H2 are added, under the induction of S2, the hairpin H1 and H2 are opened and a hybrid chain reaction occurs on the surface of the magnetic nanoparticles, and the formed long-chain DNA complexes pass...

Embodiment 2

[0035] Example 2 Oligonucleotide sequence design

[0036] The oligonucleotide sequence designed by the present invention is synthesized by China Shanghai Sangon Bioengineering Co., Ltd., and purified and tested by HPLC, and freeze-dried. The oligonucleotide sequence designed by the present invention is as follows:

[0037] S1: 5'-SH-CAC TTC TCT CTT CTC TTC CCT CTC-3';

[0038] S2: 5'-AGG AGT AGA CTA GAT CGG ACA CAC ACC CAA CAC AAC ACA CAA CTC-NH 2 -3';

[0039] H1: 5'-TGT CCG ATC TAG TCT ACT CCT ACT GTG AGG AGT AGA CAT GAT-NH 2 -3';

[0040] H2: 5'-AGG AGT AGA CTA GAT CGG ACA ATC TAG TCT ACT CCT CAC AGT-NH 2 -3';

[0041] The oligonucleotides were dissolved in ultrapure sterile water and stored at -18°C for later use.

Embodiment 3

[0042] Example 3 Fe 3 O 4 Preparation of @Au core-shell magnetic nanoparticles

[0043] Take 1 g FeCl 2 4H 2 O, 2.6 g FeCl 3 6H 2 O and 0.425 mL of concentrated HCl were dissolved in 100 mL of water and deoxygenated by sonication. The above mixture was added dropwise to 125 mL of 0.75 M NaOH solution, N 2 Stirring at 80 °C under protection to obtain Fe 3 O 4 Magnetic Nanoparticles. Prepare 5 g / L Fe 3 O 4 25 mL of ethanol solution, ultrasonically dispersed, 0.4 mL of 3-aminopropyltriethoxysilane was added dropwise, stirred at room temperature for 7 h, diluted to 1 g / L, and 14 mL of 0.6 M HAuCl was added respectively 4 3H 2 O and 0.3 mL of 0.2 M sodium citrate solution, sonicated at room temperature, the color of the solution changed from light yellow to black to obtain Fe 3 O 4 @Au core-shell magnetic nanoparticles, magnetically separated and washed, and stored dry.

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Abstract

The invention relates to a method for detecting metal ions based on a hybrid chain type reaction signal amplification technique, belonging to the technical field of analytical chemistry or environmental monitoring. The method comprises the following steps: fixing a nucleic acid chain S1 rich in C basic groups to the surfaces of gold-packaged magnetic nanoparticles through Au-S bonds by virtue of a self-assembling technique; in the presence of silver ions (Ag<+>), forming double-stranded DNA by a ferrocene labeled nucleic acid chain S2 rich in C basic groups and the nucleic acid chain S1 by virtue of a C-Ag<+>-C structure; after adding ferrocene labeled hairpin structure DNA H1 and H2, opening the hairpin structures H1 and H2 under the induction of S2, generating hybrid chain type reaction on the surfaces of the magnetic nanoparticles, and carrying out magnetic enrichment on generated compounds on the surfaces of the magnetic nanoparticles, so as to realize the amplification of electrochemical response signals. By determining the concentration of Ag<+> in a solution by virtue of the enhancement of electrochemical signals, the method has the characteristics of high sensitivity and selectivity, simplicity, rapidness and the like.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry or environmental monitoring, in particular to an electrochemical method for detecting heavy metal silver ions based on magnetic nanoparticles and hybrid chain reaction signal amplification technology. Background technique [0002] As a precious metal, silver has excellent optical, electrical and bactericidal properties, so it is widely used in various industries such as energy, photography, and medicine. Therefore, some silver-containing residues are bound to be discharged into the water environment, posing potential threats to aquatic organisms and human health. Silver ions (Ag + ) can enter the human body through the food chain or drinking water, resulting in the inactivation of sulfur-containing enzymes in the body, and can also combine with various metabolites such as amines and imidazoles to cause various diseases, such as gastroenteritis, nervous disorders, mental fatigue, rheu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 张艳丽李海燕庞鹏飞王红斌
Owner YUNNAN MINZU UNIV
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