16 results about "Nucleic Acid Strand" patented technology

This invention provides a multivalent protein-targeting degradation system based on a programmable nucleic acid template, its preparation method, and its applications, belonging to the fields of biomedicine and molecular engineering. The system includes monovalent nucleic acid degradation units and a nucleic acid template. The monovalent degradation unit is formed by hybridization of two nucleic acid chains coupled with a target protein ligand and an E3 ubiquitin ligase ligand. The nucleic acid template has n loading sites, which assemble into multivalent nucleic acid degraders through complementary base pairing. This invention achieves precise control over the spacing and configuration of monovalent units by regulating the template spacer sequence length and topology, resulting in a spatially ordered multivalent synergistic degradation effect and significantly improving the degradation efficiency of target proteins. This system is highly modular and programmable, providing a new technological platform for the development of targeted protein degradation drugs.

A label for analysing a biological sample includes a first label part including a first nucleic acid strand, and a second label part including a second nucleic acid strand. The first nucleic acid strand and the second nucleic acid strand are configured to form a duplex. The label further includes at least one first labelling moiety and at least one second labelling moiety, and the label further includes at least one blocking nucleic acid strand.

The application discloses a kind of photoactivation manganese ion bridging type deoxyribozyme probe preparation and its application in Cistanche inhibiting acetylcholinesterase activity.The probe is pSD@MnO2 Composite structure, which is composed of manganese dioxide nanosheet and functional nucleic acid assembly adsorbed on its surface, the assembly includes S chain labeled with fluorescence / quenching group and containing deoxyribozyme cleavage site, and pD chain partially hybridized with S chain and containing photocleavage site.Its preparation includes nucleic acid probe assembly, MnO2 Nanosheet complexing and purification steps.When applied, AChE catalytic product decomposes MnO2, releases Mn 2+ And nucleic acid chain; after centrifugation, pD chain is cut by ultraviolet light activation trigger, and released DNAzyme is cut under the assistance of Mn 2+ It produces amplified fluorescence signal; by comparing signal change before and after adding inhibitor, inhibition activity can be quantitatively evaluated.The application combines photocontrol activation with enzyme cascade amplification, realizes high sensitivity and operation synchronism of detection process, and is especially suitable for high-throughput rapid screening of AChE inhibitor in Cistanche and other natural products.

This invention belongs to the field of CRISPR / Cas12a system regulation technology, and discloses a method for post-activation inhibition of the CRISPR / Cas12a system without modification or additional stimulation. The core of this method is to provide a post-activation inhibition approach to address safety concerns such as runaway editing, off-target effects, and unexpected cytotoxicity caused by the long-term maintenance of high activity after CRISPR / Cas12a system activation. The core innovation of this method lies in the design of an RNA inhibitor (iRNA). This inhibitor requires no chemical modification and does not rely on external stimuli such as light, small molecule induction, or temperature changes to function, possessing advantages such as simple structure and ease of use. Its mechanism of action utilizes the conformational dynamic sensitivity of Cas12a itself, leveraging the thermodynamic advantages and kinetics of nucleic acid strand displacement reactions to induce conformational rearrangement of the activated Cas12a RNP complex, refolding it into an inactive state, thereby achieving highly efficient inhibition of the CRISPR / Cas12a system.

This invention discloses a method, apparatus, and application for the simultaneous detection of aroA1, aroA2, and aroA3. A sample tube is simultaneously irradiated with an 808nm external coaxial reflective LED light source. The photosensitizer CsYF4@Yb2O3, phototriggered, provides photoelectrons and heat to the LAMP process. The target gene serves as a template to initiate the amplification reaction, yielding a large amount of double-stranded DNA product. Signal probes are obtained by modifying CsYF4@Yb2O3 with nucleic acid strands. The detection area of ​​the photothermal detection paper captures the amplification product and signal probes in a sandwich configuration, providing a photothermal temperature change signal positively correlated with the amplification product content. The aroA1, aroA2, and aroA3 in the sample are quantified based on this temperature change signal. This invention utilizes the photoactive properties of the photosensitizer CsYF4@Yb2O3 to generate photoelectrons and photothermal effects, enabling rapid temperature control and direct molecular-level contact with the amplification system before photothermal detection, significantly improving photothermal efficiency.

A label for analysing a biological sample includes a first label part including a first nucleic acid strand and a second label part including a second nucleic acid strand. The first nucleic acid strand and the second nucleic acid strand are configured to form a duplex. The label further includes at least one first labelling moiety and at least one second labelling moiety. The label further includes at least one blocking nucleic acid strand that is at least partially complementary to one of the first nucleic acid strand and the second nucleic acid strand.

The present invention discloses a system and a method for detecting different developmental stages of fungi life cycle in postharvest crops. More particularly, the system comprises a measuring transducer, such as a CMOS photodetector and immobilized fungal nucleic acid strands. RNA from the tested postharvest produce sample is loaded onto the system of the present invention, and specific fungal mRNA transcripts anneal to the immobilized strands. A reporter strand conjugated to a signal-generating component further binds the mRNA transcripts, and when a detectable reaction is produced post binding, the transducer captures said reaction and converts it to measurable values, indicating the quantity, presence and the developmental stage of the fungus.

A nucleic acid strand cleaving method that is characterized by comprising a nucleic acid preparation step in which a nucleic acid to be cleaved that has a structure indicated in formula (1) is prepared, and a cleaving step in which the nucleic acid to be cleaved is cleaved at X in formula (1) by reacting the nucleic acid to be cleaved with a cleaving agent, and a nucleic acid that has a structure indicated in formula (2) is generated, wherein the cleaving agent is metal nanoparticles that include atoms selected from the group consisting of silver, mercury, and cadmium.　[Formula 1] (B indicates a base, and X indicates sulfur or selenium. NucA comprises at least one nucleotide, is part of the nucleic acid to be cleaved, and indicates a section on the 5'-end side, relative to X. NucB comprises at least one nucleotide, is part of the nucleic acid to be cleaved, and indicates the 3'-end side, relative to X.)

Binding complexes including a detectable nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The binding complexes allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array-based applications.

A label for analysing a biological sample is provided. The label comprises a first label part comprising a first nucleic acid strand (106) and a second label part comprising a second nucleic acid strand (110). The first nucleic acid strand (106) and the second nucleic acid strand (110) are configured to form a duplex. The label further comprises at least one first labelling moiety (108) and at least one second labelling moiety (112), and the label further comprises at least one blocking nucleic acid strand (126, 128). In further aspects, a marker (100, 500, 600, 700, 1000) comprising the label and a respective method are provided.

To provide a rapid nucleic acid amplification method. [Solution] A method for using photothermal nanoparticles in rapid nucleic acid amplification and photothermal nanoparticles comprises forming a reaction mixture comprising a nucleic acid template, a polymerase enzyme, and a heating medium. The heating medium comprises a plurality of photothermal nanoparticles suspended in a solution, fabricated on a microchip, or fabricated on the surface of a well of a multiwell plate. Photothermal nanoparticles are nanoparticles having a specific geometric shape and may be single-component or multi-component. The plurality of photothermal nanoparticles are further classified by surface plasmon resonance having a specific range of resonance wavelengths for converting energy absorbed from a light source to sufficiently heat the volume of the composition for the transfer of a new nucleic acid chain. The specific geometric shapes of the photothermal nanoparticles are selected from the group consisting of polyhedra, ellipsoids, rings, and hollow shapes.

A label for analysing a biological sample is provided. The label comprises a first label part comprising a first nucleic acid strand (106) and a second label part comprising a second nucleic acid strand (110). The first nucleic acid strand (106) and the second nucleic acid strand (110) are configured to form a duplex. The label further comprises at least one first labelling moiety (108, 710) and at least one second labelling moiety (112), and the label further comprises at least one blocking nucleic acid strand (126, 128). In further aspects, a marker (100, 500, 600, 700, 1000) comprising the label and a respective method are provided.

An apparatus for extracting nucleic acid strands from a biological sample, the apparatus comprising: a sample container for containing a biological sample in use; at least one rotatable magnetic element arranged to be located in the sample container and comprising a surface for binding nucleic acid; and a magnetic field source, wherein the apparatus is arranged such that, in use, the magnetic field source causes the at least one rotatable element to rotate and at least one nucleic acid strand to be wound around it.

The present disclosure relates to nucleic acid-peptide-nucleic acid conjugate molecules and to methods for synthesizing nucleic acid-peptide-nucleic acid conjugate molecules. In some embodiments, a method for synthesizing a nucleic acid-peptide-nucleic acid conjugate molecule using proximity-enhanced synthesis includes covalently linking a peptide with a first nucleic acid strand via a first reaction, hybridizing the first nucleic acid strand with a second nucleic acid strand to bring the second nucleic acid strand in proximity to the peptide, and covalently linking the peptide with the second nucleic acid strand via a second reaction to provide the nucleic acid-peptide-nucleic acid conjugate molecule. In some embodiments, the peptide of the nucleic acid-peptide-nucleic acid conjugate molecule is a substrate for cleavage by an enzyme, such as matrix metalloproteinase-8 (MMP-8). Exemplary applications of the nucleic acid-peptide-nucleic acid conjugate molecule for drug delivery, molecular assembly of hybrid structures, and constraining the peptide to a biologically active conformation are also disclosed.

A marker for analyzing biological samples is provided. The marker includes a first marker portion and a second marker portion, the first marker portion including a first nucleic acid chain (106) and the second marker portion including a second nucleic acid chain (110). The first nucleic acid chain (106) and the second nucleic acid chain (110) are configured to form a double strand. The marker further includes at least one first marker portion (108) and at least one second marker portion (112), and the marker further includes at least one blocking nucleic acid chain (126, 128). In other aspects, markers (100, 500, 600, 700, 1000) including the marker and corresponding methods are provided.

A marker for analyzing a biological sample is provided. The marker includes a first marker moiety and a second marker moiety, the first marker moiety including a first nucleic acid strand (106), the second marker moiety including a second nucleic acid strand (110). The first nucleic acid strand (106) and the second nucleic acid strand (110) are configured to form a duplex. The marker further includes at least one first marker moiety (108, 710) and at least one second marker moiety (112), and the marker further includes at least one blocking nucleic acid strand (126, 128). In other aspects, markers (100, 500, 600, 700, 1000) including the marker and corresponding methods are provided.