The invention provides a microfluidic-based
nucleic acid hybridization reaction platform and a microfluidic-based
nucleic acid hybridization
analysis method. The platform consists of an upper layer and a lower layer, wherein the upper layer is a
microfluidic chip controlled by a
micropump and a microvalve; the lower layer is a substrate for fixing an
oligonucleotide probe; and a fluid channel or a chamber is formed by the upper layer and the lower layer through irreversible sealing. The hybridization
analysis method comprises a step of promoting liquid flow to automatically flow to and fro in a hybridization channel by taking
positive pressure and negative pressure which are controlled by a program as a driving force, namely filling a sample into a microchannel by a capillary force or negative pressure after the sample enters a waste liquid chamber, and promoting liquid to flow to and fro in the microchannel by controlling the pushing and bounce (
positive pressure and negative pressure) of two valve seats at both ends of the liquid channel. The microfluidic-based
nucleic acid hybridization reaction platform and the microfluidic-based nucleic acid hybridization
analysis method have the advantages that:
mass transfer of materials is quick, hybridization time is short, the sample consumption is low, a hybridization
signal of the sample can be detected in real time, the integration is easy to realize, a plurality of samples can be subjected to parallel analysis synchronously and the like.