2 results about "Immobilized Oligonucleotides" patented technology

An improved nucleic acid capture method, comprising the following steps: by means of a splint oligonucleotide hybridization random probe and an oligonucleotide strand fixed on a capture chip, an RNA in a sample captured by the random probe undergoing reverse transcription into a cDNA, and linking the cDNA to the oligonucleotide strand, the reverse transcription and the linking being carried out in the same reaction system. During the process, while the RNA undergoes the reverse transcription into the cDNA, the cDNA is linked, by means of splint hybridization of fixed oligonucleotide sequences at two ends, to the oligonucleotide strand fixed on the chip. The method can increase the number of captured genes, and reduce the loss of cDNA; in addition, the method combines what was originally three steps into one step, thus further shortening the duration of the process.

The application discloses a one-step long-chain DNA parallel synthesis method based on nucleic acid framework space programming, and belongs to the fields of molecular biology and DNA nanotechnology. The method comprises the following steps: preparing a nucleic acid framework structure template with a specific template chain, wherein the nucleic acid framework structure is a two-dimensional or three-dimensional DNA assembly precisely programmed in space position; hybridizing the nucleic acid framework structure template with an oligonucleotide pool to form an assembly; and adding a nucleic acid ligase to sequentially connect the oligonucleotides, thereby obtaining a long-chain nucleic acid product. The application utilizes the nucleic acid framework structure to fix the precise position of the oligonucleotides, improves the local concentration and sequencing accuracy, and realizes one-step efficient connection. The application solves the problems of low fidelity, small flux and high cost in long-chain nucleic acid synthesis in the prior art, has the advantages of high synthesis efficiency, high fidelity, high-throughput parallel synthesis, strong scalability and the like, and is suitable for the fields of synthetic biology, genetic engineering and biological medicine research and development.