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Plastic carriers for producing biochips

A technology of slides and plastics, which is applied in the determination/inspection of microorganisms, biological tests, bioreactors/fermenters for specific purposes, etc., and can solve the interference of biochip analysis, unrevealed or prompted polystyrene plastic slides, Unfavorable problems such as high background signal

Inactive Publication Date: 2004-04-21
ADVANCED GENE TECH GORP
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  • Abstract
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Problems solved by technology

[0007] Although plastic materials have been used to make DNA chips, the currently used plastic slides are flat, that is, there are no grooves or protrusions on the upper surface, which will produce unfavorable high background signals. Interference during analysis
So far no one has successfully used surface-treated plastic slides to immobilize (preferably in the form of a microarray) proteins, peptides or small molecule compounds on the treated surface, especially when the protein, peptide or small molecule When the compound is unmodified
[0008] Furthermore, the prior art does not disclose or suggest that the surface of a polystyrene plastic slide can be treated with a simple one-step reagent to enable the immobilization of oligonucleotides or polynucleotides (preferably in a microarray format) on the treated surface. on the surface

Method used

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  • Plastic carriers for producing biochips
  • Plastic carriers for producing biochips

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Embodiment

[0029] Labeling proteins or peptides with fluorophores

[0030] Cy3-SA (i.e. Cy3-labeled Streptavidin (SA)) and Cy5-SA (ie Cy5-labeled streptavidin). Gel filtration chromatography was performed using a Bio-Gel P2 (Bio-Rad) chromatography column (0.5 cm × 10 cm) equilibrated in TBST buffer (50 mM Tris HCl, pH 7.35, containing 0.15 M NaCl) to remove untreated Coupled fluorescent residues. The estimated final concentration was 0.5 mg / ml.

[0031] Pretreatment of Plastic Slides and Preparation of Coated Plastic Slides

[0032] Both molded and flat plastic slides are made from styrene polymer. The molded plastic slides were of comparable dimensions to conventional glass slides used in microarrayers or laser scanners, and had two grooves, each 0.05 mm deep. The flat plastic slides are also comparable in size to conventional glass slides used in microarrayers or laser scanners.

[0033] Both molded plastic slides and flat plastic slides were immersed in 0.4% glutaraldehyde ...

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Abstract

A plastic carrier for preparing biochip is prepared from polystyrene and has at least one recess with 0.01-0.5 mm in depth. Its surface is chemically treated for immobilizing the protein, peptide or small-molecular compound, that is, oligonucleotide or polynucleotide, on its surface to prepare the DNA biochip.

Description

technical field [0001] The present invention relates to plastic slides that can be used in the manufacture of biochips. In particular, the present invention relates to molded plastic slides with specific configurations that can be used to prepare plastic slides with chemically treated surfaces on which biological samples for microarrays can be immobilized and spotted, thereby Make biochips. Background of the invention [0002] Glass slides have been widely used as substrates for the manufacture of DNA chips and protein chips. However, the current use of glass slides as substrates for biochips has exhibited several disadvantages. First, glass slides are inherently fragile and require careful handling. The surface of glass slides is not easily receptive to chemical treatment, and thus small biomolecular compounds, such as plant or herbal metabolites, cannot be successfully immobilized on the treated surface for the purpose of direct binding. G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/68G01N33/531G01N33/545G01N33/68
Inventor 徐立伟张素真
Owner ADVANCED GENE TECH GORP
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