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Substrate for array printing

A substrate and array technology, applied in the field of high-density biological and chemical arrays, can solve the problems of fluctuating detection results, easy migration out of the glass, not easy, etc.

Inactive Publication Date: 2001-09-26
CORNING INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] A problem with the routine use of soda lime glass as a carrier substrate for high-density arrays is the presence of particle contamination, which is common in the production of this low-grade glass
With 10,000 target sites per slide such a finely distributed sample, particle contamination can be particularly detrimental to its handling
In addition, the sodium contained in soda lime glass will easily migrate out of the glass
The resulting fogging can adversely affect glass clarity and thus interfere with the aforementioned inspection techniques
Finally, it is not easy to form uniform functionalized coatings (such as silane coatings with amino functional groups) on the surfaces of glass slides that are routinely used today.
Without a uniform coating, oligonucleotides will attach unevenly, making assay results volatile and unreliable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] A comparative study was carried out to determine the durability of 1"×3" glass slides coated with the same coating but made of 3 different glasses (soda lime glass, borosilicate glass and boroaluminosilicate glass) . For each glass slide to be tested, a coating of γ-aminopropyltriethoxysilane was applied. Then immerse the glass slide in boiling water for 0.5-5 hours. If the aminated coating remains on the surface of the glass slide after being exposed to certain environmental effects (in this case, boiling water), the functionality of the surface is said to retain its functionality, and the result of the durability test is Positive.

[0019] The durability of the aminated coating was tested by the color test method based on the Au / Ag growth method. This method can reveal the presence or absence of amine functions on the surface of the substrate. When Au / Ag growth occurs, the test result for the presence or absence of amine function is positive. At this time, a thick and uni...

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Abstract

A substrate for use in supporting high density biological or chemical arrays that is made from borosilicate or boroaluminosilicate glass. It has been demonstrated that a functionalized coating used to immobilize oligonucleotides for example, retains its functionality when exposed to environmental stresses when it is applied to a slide composed of a glass material having a low sodium oxide content.

Description

Invention field [0001] The present invention relates to high-density biological and chemical arrays, and more particularly to an improved substrate material on which the array can be deposited. Background of the invention [0002] Oligonucleotide hybridization is widely used to determine whether there is a sequence complementary to the oligonucleotide probe in the nucleic acid. In many cases, this method provides a simple, fast, and inexpensive alternative to conventional sequencing. Hybridization does not require nucleic acid cloning and purification, base-specific reactions, and tedious electrophoretic separation. The hybridization of nucleic acid probes has been successfully applied to various applications, such as analyzing genetic polymorphisms, diagnosing genetic diseases, cancer diagnosis, detecting viruses and microbial pathogens, screening clones, genomic positioning, and ordering fragment libraries. [0003] Oligonucleotide array is composed of many individual oligonucl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/551A61K38/00B01J19/00C03C3/00C03C3/089C03C3/091C03C17/22C03C17/30C07B61/00C07H21/00C12N15/09C12Q1/68C40B40/06C40B40/10C40B40/12C40B40/14C40B60/14G01N33/53G01N33/543G01N33/566G01N37/00
CPCC07H21/00B01J19/0046B01J2219/00378B01J2219/00527B01J2219/00596B01J2219/00605B01J2219/00612B01J2219/00637B01J2219/00659B01J2219/00722B01J2219/00725B01J2219/00731B01J2219/00831C03C3/089C03C3/091C03C17/30C07B2200/11C40B40/06C40B40/10C40B40/12C40B40/14C40B60/14B01J2219/00617B01J2219/00626Y10T428/24355Y10T428/31612Y10T428/31663
Inventor A·卡雷
Owner CORNING INC
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