Nucleic acid hybridized platform based on micro-flow control and hybridization analysis method thereof

Abstract

The invention provides a nucleic acid hybridized platform based on micro-flow control and a hybridization analysis method thereof; the platform comprises an upper layer and a lower layer; the upper layer is a centrifugal micro-flow controlled chip, and the lower layer is a substrate of fixed oligonucleotide probe; the upper and the lower layers are sealed to form a fluid passage or a chamber; the hybridization analysis method takes a centrifugal force and a capillary tube force as driving forces to accelerate liquid flow to flow into a hybridized passage automatically in reciprocating manner; the chip is rotated to produce the centrifugal force, and the sample liquid flow collapses under the effect of the centrifugal force; the liquid flows through the hybridized passage and enters a temporary liquid collecting tank; then the chip is stopped rotating and the sample liquid flow is returned back to the hybridized passage from the temporary liquid collecting tank under the effect of the capillary tube force; and the repeated steps of rotating and stopping chip can reciprocate the liquid flow. The invention has the advantages of fast material transmission, short hybridizing time and low consumption of sample; and multiple samples can be analyzed in parallel simultaneously.

Description

technical field [0001] The invention relates to nucleic acid hybridization analysis on a centrifugal microfluidic nucleic acid hybridization platform based on reciprocating flow, using centrifugal force and capillary force as the driving force to promote the liquid flow to reciprocate automatically in the hybridization channel, thereby accelerating the reaction rate of nucleic acid hybridization, Shorten the hybridization reaction time. Background technique [0002] Nucleic acid hybridization has been widely used in gene expression analysis, gene phenotype analysis, clinical disease diagnosis and other fields. Nucleic acid hybridization techniques are based on the specific binding of complementary DNA strands. For the traditional static nucleic acid hybridization mode represented by microarray chips, the hybridization process relies solely on diffusion. The target molecule must diffuse from the solution to the surface of the probe immobilized on the support to interact with...

AI Technical Summary

Problems solved by technology

Although the continuous flow of the sample speeds up the mass transfer of the material, the consumption of the sample is relatively large

Some people use peristaltic pumps, magnetic stirring, and manual operation to drive samples to circulate in the hybridization area, but these chips not only require relatively expensive external auxiliary equipment or manual operation, but also cannot achieve parallel multi-sample analysis

Images