Manufacturing method and application method of Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode

A technology of alloy nanoparticles and glucose sensor, which can be used in instruments, scientific instruments, electrochemical variables of materials, etc., and can solve the problems of high cost, poor selectivity, and easy deactivation.
CN102435649AInactive Publication Date: 2012-05-02TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2012-05-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode and a manufacturing method and an application method thereof. The manufacturing method for the electrode comprises the following steps of: polishing a stainless steel acupuncture needle on an animal skin; repeatedly washing by secondary distilled water and ultrasonically processing for 5-10min in an ultrasonic washer with power of 100-150W; placing in a phosphate buffered solution at pH7 and activating for 10-15 circles through cyclic sweep voltammetry; dissolving 49-60mg of chitosan in 80-120ml of 0.05mol / L of acetic acid solution to obtain 0.4-0.6% of chitosan solution; and dissolving the manufactured Pt-Ag alloy nanoparticle in 0.5ml of 0.4-0.6% of chitosan solution, ultrasonically dispersing, dispensing 40-60 mu l of the ultrasonically dispersed solution onto an Au electrode, and drying at room temperature. The Pt-Ag alloy nanoparticle electrode has good catalytic activity, excellent electrocatalytic activity for glucose oxidation, wide linear response range and high sensitivity and selectivity.
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Description

technical field

[0001] The invention relates to the preparation and application of a Pt-Ag alloy nano particle enzyme-free glucose sensor electrode. Background technique

[0002] Electrochemical glucose biosensor has the characteristics of high selectivity, rapidity and simplicity, and is the most important method for detecting glucose concentration. In recent years, the incidence of diabetes has continued to increase. In order to provide diabetics with more efficient means of detecting blood sugar, the development of new glucose sensors has received extensive attention. Although the enzyme-based sensor has good selectivity and sensitivity in the monitoring process, the enzyme is easily inactivated and denatured during the immobilization process, so the biggest problem with the enzyme-based sensor is poor stability; the second is the amount of immobilized enzyme It cannot be accurately controlled, so that the repeatability of the prepared sensor cannot be guaranteed. Nanom...

Claims

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