Manufacturing method and application method of Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode

A technology of alloy nanoparticles and glucose sensor, which can be used in instruments, scientific instruments, electrochemical variables of materials, etc., and can solve the problems of high cost, poor selectivity, and easy deactivation.

Inactive Publication Date: 2012-05-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most enzyme-free electrodes have defects such as low sensitivity, poor selectivity, high cost and easy deactivation.

Method used

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  • Manufacturing method and application method of Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode
  • Manufacturing method and application method of Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode
  • Manufacturing method and application method of Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 0.04×10 in 0.8ml -3 mol / LAgNO3 and 8ml of 0.1mol / L polyvinylpyrrolidone (PVP) were dissolved in 20ml of distilled water, stirred for 30 minutes, then added to 0.8ml of 1mol / L ascorbic acid (AA), and stirred to fully react. Centrifuge at 10000r / min for 15 minutes, wash with distilled water repeatedly to remove remaining PVP. Dissolve the above-prepared Ag nanoparticles in 0.5 ml of distilled water, take 50 μl and dissolve them in 4.8 ml of 0.1×10 -3 mol / L chloroplatinic acid, reacted at room temperature for 72 hours. The Au electrode was ground and polished on buckskin, rinsed repeatedly with twice distilled water, ultrasonicated for 5 minutes in an ultrasonic cleaner with a power of 100 watts, placed in PBS with a pH of 7.0, and activated by cyclic voltammetry scanning for 10 laps for use. Dissolve 50 mg of chitosan in 100 ml of 0.05 mol / L acetic acid solution to form a 0.4% chitosan solution (CS). After dissolving the Pt-Ag alloy nanoparticles prepared in (2) in 0.5...

Embodiment 2

[0028] 0.7ml of 0.04×10 -3 mol / L AgNO3 and 6ml of 0.1mol / L polyvinylpyrrolidone (PVP) were dissolved in 20ml of distilled water, stirred for 20 minutes, then added into 0.6ml of 1mol / L ascorbic acid (AA), stirred to make it fully react. Centrifuge at a rotational speed of 12000r / min for 10 minutes, and wash with distilled water repeatedly to remove the remaining PVP. Dissolve the above-prepared Ag nanoparticles in 0.5 ml of distilled water, take 50 μl and dissolve them in 3.2 ml of 0.1×10 -3 mol / L chloroplatinic acid, reacted at room temperature for 60 hours. The Au electrode was polished on the chicken skin, rinsed repeatedly with twice distilled water, ultrasonicated in an ultrasonic cleaner with a power of 120 watts for 8 minutes, placed in PBS with a pH of 7.0, and activated by cyclic voltammetry scanning for 12 laps for use. 40 mg of chitosan was dissolved in 80 ml of 0.05 mol / L acetic acid solution to form a 0.5% chitosan solution (CS). After dissolving the Pt-Ag allo...

Embodiment 3

[0030] 0.9ml of 0.04×10 -3 mol / L AgNO3 and 10ml of 0.1mol / L polyvinylpyrrolidone (PVP) were dissolved in 20ml of distilled water, stirred for 40 minutes, then added into 1.0ml of 1mol / L ascorbic acid (AA), and stirred to fully react. Centrifuge at a rotational speed of 15000r / min for 8 minutes, and wash with distilled water repeatedly to remove the remaining PVP. Dissolve the above-prepared Ag nanoparticles in 0.5 ml of distilled water, take 50 μl and dissolve them in 5.4 ml of 0.1×10 -3 mol / L chloroplatinic acid, reacted at room temperature for 80 hours. The Au electrode was ground and polished on buckskin, rinsed repeatedly with double distilled water, ultrasonicated for 10 minutes in an ultrasonic cleaner with a power of 150 watts, placed in PBS with a pH of 7.0, and activated by cyclic voltammetry scanning for 15 cycles for use. 60 mg of chitosan was dissolved in 120 ml of 0.05 mol / L acetic acid solution to form a 0.6% chitosan solution (CS). After dissolving the Pt-Ag ...

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Abstract

The invention relates to a Pt-Ag alloy nanoparticle enzyme-free glucose sensor electrode and a manufacturing method and an application method thereof. The manufacturing method for the electrode comprises the following steps of: polishing a stainless steel acupuncture needle on an animal skin; repeatedly washing by secondary distilled water and ultrasonically processing for 5-10min in an ultrasonic washer with power of 100-150W; placing in a phosphate buffered solution at pH7 and activating for 10-15 circles through cyclic sweep voltammetry; dissolving 49-60mg of chitosan in 80-120ml of 0.05mol / L of acetic acid solution to obtain 0.4-0.6% of chitosan solution; and dissolving the manufactured Pt-Ag alloy nanoparticle in 0.5ml of 0.4-0.6% of chitosan solution, ultrasonically dispersing, dispensing 40-60 mu l of the ultrasonically dispersed solution onto an Au electrode, and drying at room temperature. The Pt-Ag alloy nanoparticle electrode has good catalytic activity, excellent electrocatalytic activity for glucose oxidation, wide linear response range and high sensitivity and selectivity.

Description

technical field [0001] The invention relates to the preparation and application of a Pt-Ag alloy nano particle enzyme-free glucose sensor electrode. Background technique [0002] Electrochemical glucose biosensor has the characteristics of high selectivity, rapidity and simplicity, and is the most important method for detecting glucose concentration. In recent years, the incidence of diabetes has continued to increase. In order to provide diabetics with more efficient means of detecting blood sugar, the development of new glucose sensors has received extensive attention. Although the enzyme-based sensor has good selectivity and sensitivity in the monitoring process, the enzyme is easily inactivated and denatured during the immobilization process, so the biggest problem with the enzyme-based sensor is poor stability; the second is the amount of immobilized enzyme It cannot be accurately controlled, so that the repeatability of the prepared sensor cannot be guaranteed. Nanom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 许鑫华路会冉郭美卿侯红帅方海东
Owner TIANJIN UNIV
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