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Preparation and application of Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode

A glucose sensor and nanoparticle technology, applied in the direction of electrochemical variables of materials, can solve problems such as poor selectivity and loss of glucose electrocatalytic oxidation activity, and achieve the effects of large current response, wide detection linear range, and simple and easy preparation method.

Active Publication Date: 2014-02-26
北京传奇优声文化传媒有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Platinum (Pt) is one of the most commonly used electrode materials in enzyme-free glucose sensors, but Pt electrodes can catalyze the oxidation of many carbohydrates at the same potential, but the selectivity to catalytic oxidation of glucose is poor; Pt electrodes are easily affected by Poisoning of chloride ions and loss of electrocatalytic oxidation activity for glucose

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  • Preparation and application of Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode
  • Preparation and application of Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode
  • Preparation and application of Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode

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Embodiment 1

[0032]Grind and polish the Au electrode on the animal skin, rinse it repeatedly with twice distilled water, and ultrasonicate for 5 minutes in an ultrasonic cleaner with a power of 100-150 watts, then place it in PBS with a pH of 7.0 and activate it by cyclic voltammetry scanning for 10 cycles spare. First, 50 μl of 0.1mol / L CuCl 2 With 50 μl of 1mol / L sodium citrate (C 6 h 5 o 7 Na 3 2H 2 O) Dissolve in 20ml of double distilled water, stir for 5 minutes, then continue to stir, inject 1ml 0.025mol / L sodium borohydride (NaBH 4 ), and stirred for 15 minutes. Cu-Pt alloy nanoparticles were prepared by the method of current displacement, that is, 3.2ml of 0.1×10 -3 mol / L chloroplatinic acid. Then, after stirring and reacting for 20 minutes, the solution was reacted statically at room temperature (14±2°) for 24 hours. Centrifuge at a rotational speed of 12000r / min for 15 minutes, and repeatedly wash with double distilled water to completely remove remaining unreacted subst...

Embodiment 2

[0034] First, 50 μl of 0.1mol / L CuCl 2 With 60μl 0.1mol / L sodium citrate (C 6 h 5 o 7 Na 3 2H 2 O) Dissolved in 25ml of double distilled water, stirred for 6 minutes, then continued to stir, and quickly injected 1.25ml of 0.025mol / L sodium borohydride (NaBH 4 ), and stirred for 10 minutes. Cu-Pt alloy hollow nanoparticles were prepared by current displacement method, that is, 4.8ml of 0.1×10 -3 mol / L chloroplatinic acid. Then, after stirring and reacting for 15 minutes, the solution was reacted statically at room temperature (14±2°) for 36 hours. The Au electrode was polished on the chicken skin, rinsed repeatedly with twice distilled water, ultrasonicated in an ultrasonic cleaner with a power of 120 watts for 8 minutes, placed in PBS with a pH of 7.0, and activated by cyclic voltammetry scanning for 12 laps for use. Centrifuge at a rotational speed of 12000r / min for 20 minutes, and repeatedly wash with double distilled water to completely remove remaining unreacted su...

Embodiment 3

[0036] First, 50 μl of 0.1mol / L CuCl 2 With 70μl of 0.1mol / L sodium citrate (C 6 h 5 o 7 Na 3 2H 2 O) be dissolved in 30ml of double distilled water, after stirring for 8 minutes, then continue to stir, inject 1.5ml 0.025mol / L sodium borohydride (NaBH 4 ), and stirred for 15 minutes. Cu-Pt alloy hollow nanoparticles were prepared by current displacement method, that is, 5.4ml of 0.1×10 -3 mol / L chloroplatinic acid. Then, after stirring and reacting for 20 minutes, the solution was reacted statically at room temperature (14±2°) for 32 hours. The Au electrode was polished on the chicken skin, rinsed repeatedly with double distilled water, ultrasonicated for 10 minutes in an ultrasonic cleaner with a power of 120 watts, and then placed in PBS with a pH of 7.0 and activated by cyclic voltammetry scanning for 15 cycles for use. Centrifuge at a rotational speed of 12000r / min for 18 minutes, and repeatedly wash with double distilled water to completely remove remaining unreac...

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Abstract

The invention relates to a Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode, and preparation and application thereof. The preparation method of the electrode comprises the following steps: sanding and polishing an Au electrode on a hide, repeatedly washing with redistilled water, carrying out ultrasonic treatment for 5-10 minutes in an ultrasonic cleaner of which the power is 100-150W, putting in a phosphate buffer solution of which the pH value is 7, and activating for 10-15 cycles by cyclic volt-ampere scanning; dissolving 40-60mg of chitosan in 80-120ml of 0.05 mol / L acetic acid solution to obtain a 0.4-0.6% chitosan solution; and dissolving the prepared Pt-Cu alloy nanoparticles in 0.5ml of 0.4-0.6% chitosan solution, carrying out ultrasonic dispersion, dropwisely coating 40-60 mu l on the Au electrode, and drying at room temperature. The Pt-Cu alloy nanoparticle electrode has the advantages of favorable catalytic activity, favorable electrocatalytic activity for glucose oxidization, wide linear response range, high sensitivity and high selectivity.

Description

technical field [0001] The invention relates to the preparation and application of a Pt-Cu alloy hollow nanoparticle enzyme-free glucose sensor electrode Background technique [0002] Glucose is one of the most basic characteristic chemicals in the process of human life. The detection and analysis of glucose is of vital significance in the diagnosis, treatment and control of human health and diseases. Therefore, how to develop fast, accurate, low-cost, convenient and effective technology to detect glucose has become a research hotspot. The most common biosensor for detecting glucose is the glucose enzyme biosensor, which uses the characteristics of high specificity and fast reaction speed of the enzyme to make the sensor have good selectivity and high sensitivity. However, due to the inherent nature of the enzyme, the catalytic activity of glucose oxidase is easily affected by environmental conditions (temperature, temperature, pH, toxic chemicals, etc.). Therefore, the po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
Inventor 许鑫华石永倩侯红帅方海东唐晓娜
Owner 北京传奇优声文化传媒有限公司
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