Reusable enzyme-free glucose detection device and method

A detection device, glucose technology, applied in the direction of material electrochemical variables, etc., can solve the problems of enzyme instability, lack of long-term stability, easy inactivation, etc., and achieve the effect of simple equipment, rapid detection, and improved detection limit

Inactive Publication Date: 2013-11-27
NORTHEASTERN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although enzymatic detection usually shows good selectivity and high sensitivity, due to the inherent instability of the enzyme and...

Method used

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  • Reusable enzyme-free glucose detection device and method
  • Reusable enzyme-free glucose detection device and method
  • Reusable enzyme-free glucose detection device and method

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Embodiment 1

[0031] The reusable enzyme-free glucose detection device of the present invention, such as figure 1 As shown, the electrolytic cell is fixed on the horizontal platform, so that the bottom of the electrolytic cell and the horizontal platform are integrated, and a conical hole penetrates the bottom of the electrolytic cell and the horizontal platform, and the direction of the cone top of the conical hole is vertically downward , the minimum diameter of the cone-shaped circular hole is 10 mm, and a fixed bracket is provided across the top of the electrolytic cell. The titanium dioxide nanotube array film modified with platinum nanoparticles on the titanium substrate is used as the working electrode, and the platinum sheet is used as the counter electrode. / AgCl is used as the reference electrode to form a three-electrode system. The distance between the electrodes is 6mm. It is horizontally fixed at the bottom of the round hole of the electrolytic cell, and its position is oppos...

Embodiment 2

[0037] The titania nanotube array film modified with platinum nanoparticles on the titanium substrate prepared in Example 1 was used as the working electrode, the platinum sheet was used as the counter electrode, and Ag / AgCl was used as the reference electrode in a three-electrode system, and the distance between the electrodes was 6 mm. This three-electrode system works in an electrolytic cell, and 50 mL of H with a concentration of 1 mol / L is added to the electrolytic cell. 2 SO 4 The solution is used as the electrolyte, and the Pt electrode, working electrode and reference electrode are connected to an electrochemical analyzer CHI660E. The detection conditions are as follows: the magnetic stirring rate is 200 rpm, and the voltage range used is -0.2-1.0V. Glucose solutions with different concentrations were detected by cyclic voltammetry. After the detection is completed, the detection function can be recovered by irradiating with ultraviolet light for 30 minutes.

[0038...

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Abstract

The invention relates to a reusable enzyme-free glucose detection device and method and belongs to the field of electrochemical biological sensors. A sensor provided by the invention adopts a three-electrode system, wherein a titanium dioxide nanotube array film which modifies platinum nanoparticles serves as a working electrode, a platinum sheet serves as a counter electrode and an Ag/AgCl or 3 mol/L of KCl serves as a reference electrode; the three-electrode system works in an electrolytic tank, an H2SO2 solution with the concentration of 1 mol/L serving as electrolyte is added into the electrolytic tank, and the Pt electrode, the working electrode and the reference electrode are connected with an electrochemical analysis instrument respectively, so that detection can be performed; after detection is finished, a detection function can recover after irradiation is performed for 30 minutes under ultraviolet light; the lower limit of glucose detection is 4*10<-5> mmol/L. Compared with a traditional enzyme method, the enzyme-free detection method has the advantages that the detection limit is increased by above 90%. A novel, simple, convenient, quick, low-cost, stable and reusable enzyme-free glucose detection method is provided.

Description

technical field [0001] The invention belongs to the field of electrochemical biological detection devices, in particular to a reusable enzyme-free glucose detection device and method. Background technique [0002] Glucose plays an important role in the metabolism of the human body. The urine of normal people generally does not contain free glucose. The content of glucose in the urine of diabetic patients varies greatly. The determination of free glucose in blood or urine is a routine clinical test. important item. At present, the detection of glucose by most amperometric biosensors is based on the electrochemical detection by reacting with specific oxidases to release hydrogen peroxide. Although enzymatic detection usually shows good selectivity and high sensitivity, due to the inherent instability and inactivation of enzymes, the most serious problem facing this type of detection device is the lack of long-term stability. The invention provides a novel, simple, fast, low-...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 宋焱焱代亮高志达
Owner NORTHEASTERN UNIV
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