Three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof

A glucose sensor and nanoflower technology, applied in the direction of electrochemical variables of materials, can solve the problems of not easy to generate response current, slow overall kinetics, low sensitivity, etc., and achieve strong anti-interference ability, wide linear range, and good application prospects. Effect

Active Publication Date: 2011-02-16
苏州盛泽科技创业园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The research on enzyme-free sensors is mainly based on platinum-based ampere-type glucose sensors. The kinetics and mechanism of electrochemical oxidation of glucose on bare platinum electrodes have been studied in depth. There are three defects in the process of glucose oxidation: one is that the overall kinetics of this oxidation is too slow to generate a significant response current; The activity is greatly reduced; the third is the influence of interfering substances such as ascorbic acid, uric acid and acetaminophen in the receptor, and its sensitivity is not high
At present, there is no research report on the preparation of three-dimensional Pt-Pb nanoflowers for needle-type enzyme-free glucose sensor electrodes.

Method used

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  • Three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof
  • Three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof
  • Three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Stainless steel acupuncture needles (0.35mm×15mm, Tianjin Yipeng Medical Instrument Co., Ltd.) were taken, rinsed repeatedly with twice distilled water, ultrasonicated, placed in PBS buffer solution with pH=7.0, and activated by cyclic voltammetry for 10 cycles. Electrolyte composition: the concentration of chloroplatinic acid is 3.3×10 -3 mol / L, the concentration of lead acetate is 1.65×10 -3 mol / L, the concentration of hydrochloric acid is 0.5mol / L. A three-electrode system was used in the experiment, with pretreated stainless steel acupuncture needles as working electrodes, platinum electrodes as auxiliary electrodes, and saturated calomel electrodes as reference electrodes. During the electrodeposition process, the electrolyte beaker was placed in ultrasonic vibration (100 watts), first deposited at a constant current of 10 mA for 10 min, and then deposited at a constant potential of -0.1 V for 6 min, taken out, rinsed with double distilled water, and dried. The s...

Embodiment 2

[0026] Stainless steel acupuncture needles (d=0.35 mm) were taken, rinsed repeatedly with twice distilled water, ultrasonicated, placed in PBS buffer solution with pH=7.0, and activated by cyclic voltammetry scanning for 10 cycles. Electrolyte composition: the concentration of chloroplatinic acid is 3.3×10 - 3 mol / L, the concentration of lead acetate is 1.65×10 -3 mol / L, the concentration of hydrochloric acid is 0.5mol / L. A three-electrode system was used in the experiment, with pretreated stainless steel acupuncture needles as working electrodes, platinum electrodes as auxiliary electrodes, and saturated calomel electrodes as reference electrodes. During the electrodeposition process, the electrolyte beaker was placed in ultrasonic vibration (100 watts), first deposited at a constant current of 10 mA for 6 min, and then deposited at a constant potential of -0.1 V for 6 min, taken out, rinsed with twice distilled water, and dried. The electrochemical test adopts a three-ele...

Embodiment 3

[0028] Stainless steel acupuncture needles (d=0.35 mm) were taken, rinsed repeatedly with twice distilled water, ultrasonicated, placed in PBS buffer solution with pH=7.0, and activated by cyclic voltammetry scanning for 10 cycles. Electrolyte composition: the concentration of chloroplatinic acid is 3.3×10 - 3 mol / L, the concentration of lead acetate is 1.65×10 -3mol / L, the concentration of hydrochloric acid is 0.5mol / L. A three-electrode system was used in the experiment, with pretreated stainless steel acupuncture needles as working electrodes, platinum electrodes as auxiliary electrodes, and saturated calomel electrodes as reference electrodes. During the electrodeposition process, the electrolyte beaker was placed in ultrasonic vibration (100 watts), first deposited at a constant current of 0.1mA for 10 minutes, and then deposited at a constant potential of -0.1V for 10 minutes, taken out, rinsed with twice distilled water, and dried . The electrochemical test adopts a...

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Abstract

The invention relates to a three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof. The electrode consists of a three-dimensional Pt-Pb nano floricome array on a stainless steel acupuncture needle substrate, wherein three-dimensional Pt-Pb nano floricomes exist on the surface of the electrode in a good crystallization form, and the diameter of the single nano floricome is 50-80nm. The preparation method comprises the following steps of: polishing a stainless steel acupuncture needle on deerskin; after the stainless steel acupuncture needle is repeatedly washed through secondary distilled water and processed through ultrasound, putting the stainless steel acupuncture needle in a PBS (Phosphate Buffer Solution) of whichthe pH is 7.0 to scan and activate for 10 circles through cyclic voltammetry; putting the stainless steel acupuncture needle into a hydrochloric acid electrolyte of 0.5mol / L, which consists of chloroplatinic acid and lead acetate by a ratio of 1:1; and preparing an electrode sample through an ultrasonic oscillation electro-deposition method. The three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode has good electro-catalysis activity, wide linear response range and high sensitivity and selectivity.

Description

technical field [0001] The invention relates to a three-dimensional Pt-Pb nano-flower needle type enzyme-free glucose sensor electrode and its preparation and application. Background technique [0002] In recent decades, research on electrochemical biosensors with immobilized glucose oxidase has achieved certain results, and has made great contributions to the invasive and noninvasive detection of blood glucose in diabetic patients. However, for the diagnosis and treatment of diabetes, the most important thing is the reproducible, accurate, fast, selective and cheap blood glucose monitoring in biological fluids. Although the enzyme-based sensor has good selectivity and sensitivity in the monitoring process, the enzyme is easily inactivated and denatured during the immobilization process, so the biggest problem with the enzyme-based sensor is poor stability; the second is the amount of immobilized enzyme It cannot be accurately controlled, so that the repeatability of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C25D3/50G01N27/30C25D5/20C25D7/00
Inventor 许鑫华郭美卿王蕊路会冉
Owner 苏州盛泽科技创业园发展有限公司
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