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Multi-microelectrode integrated sensing chip and application in biochemical enzyme-free rapid detection

A sensor chip and microelectrode technology, applied in the field of electrochemical detection, can solve the problems of electrochemical sensors that are difficult to reuse, enzymes that are sensitive to the external environment, and easy to deactivate

Pending Publication Date: 2021-07-30
广州钰芯传感科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The biggest limitation of the above technologies is that enzymes are extremely sensitive to the external environment and are easily inactivated, making it difficult to reuse enzyme-containing electrochemical sensors.
The invention provides an electrochemical non-enzyme sensor chip, which is applied to multi-parameter electrochemical detection to realize the purpose of detecting multiple material indicators at one time, avoiding the use of biologically active enzymes, and overcoming the problem of easy inactivation of enzymes. problem, has the advantages of good stability, low cost, simplicity and good reproducibility

Method used

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  • Multi-microelectrode integrated sensing chip and application in biochemical enzyme-free rapid detection
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  • Multi-microelectrode integrated sensing chip and application in biochemical enzyme-free rapid detection

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Effect test

Embodiment 1

[0068] A detection area is set on the front of the microelectrode integrated sensor chip, and multiple microelectrode modules are set in the detection area. Specific electrodes, wherein the distance between the working electrode and the counter electrode in the microelectrode module is 0.20-0.25 mm, and the distance between the working electrodes is 1.0-1.5 mm. The working electrode includes an electrode layer and a modified layer, wherein the electrode layer includes an electrode base Cu layer and a conductive inner layer of Ni layer from bottom to top, the thickness of the Cu layer is 10.0-15.0 μm, and the thickness of the Ni layer is 2.0-5.0 μm.

[0069] Modify the nanoporous copper modification layer on the surface of the working electrode, specifically electroplating copper-tin alloy on the surface of the microelectrode, with a current density of 5A / dm 2 , electrodeposited at a temperature of 35°C for 550s, and then put it into a 10% sulfuric acid solution for dealloying ...

Embodiment 2

[0071] A detection area is set on the front of the microelectrode integrated sensor chip, and multiple microelectrode modules are set in the detection area. Specific electrodes, wherein the distance between the working electrode and the counter electrode in the microelectrode module is 0.20-0.25 mm, and the distance between the working electrodes is 1.0-1.5 mm. The working electrode includes an electrode layer and a modified layer, wherein the electrode layer includes an electrode base Cu layer and a conductive inner layer of Ni layer from bottom to top, the thickness of the Cu layer is 10.0-15.0 μm, and the thickness of the Ni layer is 2.0-5.0 μm.

[0072] Modify the nanoporous gold modification layer on the surface of the working electrode, specifically, electroplate gold-tin alloy on the surface of the microelectrode for 5 minutes, and then place it in a 2M NaOH+1M NaCl mixed solution for dealloying treatment, using platinum as the counter electrode, and the voltage Set to ...

Embodiment 3

[0074] A detection area is set on the front of the microelectrode integrated sensor chip, and multiple microelectrode modules are set in the detection area. Specific electrodes, wherein the distance between the working electrode and the counter electrode in the microelectrode module is 0.20-0.25 mm, and the distance between the working electrodes is 1.0-1.5 mm. The working electrode includes an electrode layer and a modification layer, wherein the electrode layer includes an electrode substrate Cu layer, a conductive inner layer of Ni layer and a reaction layer Au layer from bottom to top. The thickness of the Cu layer is 10.0-15.0 μm, and the thickness of the Ni layer is 2.0 ~5.0μm, the Au layer thickness is 0.05~0.10μm.

[0075]Modify the nanoporous silver modification layer on the surface of the working electrode, specifically, electroplate silver-tin alloy on the surface of the microelectrode, keep stirring at room temperature for 5 minutes, and then put it into 4M KOH+1M ...

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Abstract

The invention provides a multi-microelectrode integrated sensing chip and application of the multi-microelectrode integrated sensing chip in biochemical enzyme-free rapid detection. According to the invention, microelectrode modules with counter electrodes as centers and a plurality of working electrodes and reference electrodes distributed on the peripheries are designed, a plurality of microelectrode modules are integrated in a detection area on the front surface of a sensing chip, electrode contacts are arranged in an electrode contact area on the back surface of the sensing chip according to the arrangement of electrodes of the microelectrode modules, the microelectrodes and the electrode contactsare subjected to one-to-one conduction connection, and non-enzyme detection of specific biochemical molecules is realized by modifying the surface of the microelectrode with a specific modification layer and then applying a specific potential.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, and in particular relates to a multi-microelectrode integrated sensor chip and its application in biochemical enzyme-free rapid detection. Background technique [0002] The real-time detection of POCT has the characteristics of fast, convenient, and cost-saving. The specific performance is that the detection results can be obtained immediately, the cost of single-body inspection is reduced, and the requirement of obtaining accurate inspection results in the shortest time is met. widely used. [0003] At present, the latest POCT chemical sensing instrument products can realize the detection of multiple samples at the same time, which greatly saves the detection time and makes the instant detection faster. POCT devices are mostly realized by biosensors. By immobilizing biological enzyme molecules on the solid phase interface of microanalytical devices, the analytes are specifically identif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/307G01N27/301G01N27/3277G01N27/3278G01N27/48
Inventor 奚亚男胡保帅崔皓博
Owner 广州钰芯传感科技有限公司
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