Prussian blue-based enzyme-free uric acid sensor and preparation method thereof

Abstract

The present invention provides a Prussian blue-based enzyme-free uric acid sensor with high detection precision, a high speed and stable performance, and a Prussian blue-based enzyme-free uric acid sensor preparation method applicable for mass industrial production. The enzyme-free uric acid sensor comprises a substrate and a working electrode on the substrate. The working electrode has Prussian blue carbon slurry, and the mass fraction of the Prussian blue in the Prussian blue carbon slurry is 0.5% to 5%. The enzyme-free uric acid sensor preparation method sequentially comprises the followingsteps: 1) adding Prussian blue powder into the carbon slurry for mixing to prepare Prussian blue carbon slurry, wherein the mass fraction of Prussian blue in the Prussian blue carbon slurry is 0.5% to 5%; 2) brushing the Prussian blue carbon slurry on the substrate to form the Prussian blue carbon slurry coating, and taking the Prussian blue carbon slurry coating as the working electrode; and 3)performing drying, wherein the drying temperature is 80 DEG C to 100 DEG C, and the drying time is 8-15 min.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to an enzyme-free uric acid sensor based on Prussian blue and a preparation method thereof. Background technique [0002] The following background art is provided to help the reader understand the present invention and is not to be admitted as prior art. [0003] At present, the detection principle of the electrode is mostly to modify the enzyme corresponding to the substrate on the working electrode, and measure the electrical signal through the enzymatic reaction, so the uric acid sensor generally uses the uricase method. However, the immobilization conditions of the enzyme on the working electrode are relatively harsh, and the activity of the enzyme needs to be kept unaffected during the immobilization process, and the temperature and humidity of the enzyme immobilization need to be considered. In addition, the price of uricase is generally all expensive, which limits its scope o...

AI Technical Summary

Problems solved by technology

The disadvantages of this patent are: 1. It is necessary to form a working electrode on the substrate first, and then coat the Prussian blue / N-doped carbon nanocomposite material on the working electrode, and the process is complicated; and the Prussian blue / N-doped carbon nanocomposite The material is easy to fall off from the surface of the working electrode, resulting in the failure of the electrode sheet

2. The preparation steps of Prussian blue / N-doped carbon nanocomposites are complicated, which increases the production cost

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