60 results about "Bac clone" patented technology

The invention relates to a method for the determination of a genome sequence comprising the steps of providing a physical map of a sample genome by sequencing fragment ends of pooled BAC clones; providing a set of sequence reads from a sample genome generating a contig of the physical map and the sequence reads.

The invention discloses a probe assembly for diagnosing ACTA2-MITF translocation perivascular epithelioid cell tumor and an application thereof. The probe assembly is formed by a BAC clone probe RP11-28G22 and a BAC clone probe RP11-963H1, wherein the BAC clone probe RP11-28G22 is located on one side of the centromere of ACTA2 and labeled with fluorescence of any color; the BAC clone probe RP11-963H1 is located on one side of the MITF telomere and labeled with fluorescence different from that labeled on the other side of the ACTA2 centromere. As the probe combination carry out in-situ hybridization on the basis of the paraffin-embedded tissue slice to detect the fusion and separation signals, the accuracy rate of diagnosis of such tumors can be greatly improved.

The invention relates to an AML1 gene and ETO gene detection probe and a preparation method thereof. The method includes the following steps that a selected BAC clone for an AML1 gene is at least one of CTD-3245J1, RP11-77G18, CTD-2349F18, CTD-3171K21 and RP11-384N13, a selected BAC clone for an ETO gene is at least one of RP11-1107H4, RP11-302P1, CTD-2547C5, RP11-55J5 and CTD-2079N17, the DNA of a plasmid is obtained, and marking is conducted. The invention further discloses a reagent kit provided with the AML1 gene and ETO gene detection probe. By obtaining the optimal AML1 gene and ETO detection probe through screening, signal line counting is accurate and quick, and result repeatability is good.

The invention relates to a BCR gene and ABL gene detection probe and a preparation method thereof. The method includes the following steps that a selected BAC clone for a BCR gene is at least one of RP11-1026A5, RP11-165G5 and CTD-2079I4, a selected BAC clone is at least one of CTD-2302P22, CTD-2037J11 and CTD-2509L4, a selected BAC clone for an ABL is at least one of CTD-2037L19, RP11-21G10 and CTD-2526G20, the DNA of a plasmid is obtained, and marking is conducted. The invention further discloses a reagent kit provided with the BCR gene and ABL gene detection probe. By obtaining the optimal BCR gene and ABL detection probe through screening, signal line counting is accurate and quick, and result repeatability is good.

The invention discloses a leukemia MEF2D gene disruption probe detection kit. The present invention provides a fluorescent in-situ hybridization polyclonal separating probe for detecting chromosomal MEF2D gene disruption, which is composed of by two BAC clone fragments (RP11-964F7 and RP11-139I14) located on the centromere side of the chromosomal MEF2D gene and two BAC clone fragments (RP11-214H6and RP11-1047J23) located on the telomere side of the chromosomal MEF2D gene. The kit utilizes a FISH technology to detect leukemia associated with MEF2D gene disruption, and performs individualized treatment for patients. The probe of the invention can comprehensively detect all translocations involving the MEF2D gene, finds new translocations, has high application accuracy, high specificity, high success rate, strong fluorescence signal and simple operation, and can assist in the optimization of the treatment and prognosis of leukemia associated with the MEF2D gene disruption.

The invention relates to methods and compositions for producing biochips as well as to the use of these biochips in diverse fields, from functional genomics to diagnosis, for example, particularly in research or in the medical field. The invention also relates to tools and methods for selecting polynucleotides that permit the production of improved biochips. The inventive biochips contain, in particular, BAC clones.

The invention relates to a TOP2A gene detection probe and a preparation method thereof. The method includes the following steps that a selected BAC clone is at least one of RP11-1152A10 and CTD-3217C5, or a selected BAC clone is at least one of RP11-737D6, CTD-3087O22, RP11-48O10 and CTD-2134K5, a plasmid is extracted from the clone to obtain DNA of the plasmid, and quantification is conducted; marking is carried out with fluorescein. The invention further discloses a reagent kit provided with the TOP2A gene detection probe. By obtaining the optimal TOP2A detection probe through screening, signal line counting is accurate and quick, and result repeatability is good. Defects of TOP2A mutation detection in clinic are made up for, more patients benefited from targeted drug can be screened more easily, the survival rate of the patients is increased, and overall life time is prolonged.

The invention discloses a reagent for detecting a copy number of an EGFR gene and the ploidy of a chromosome 7. The reagent comprises a fluorescence probe RP11-339F13 used for detecting the copy number of the EGFR gene and a fluorescence probe RP11-144H20 used for detecting the ploidy of the chromosome 7, wherein the RP11-339F13 is cloned by BAC, which covers the whole EGFR gene, and marked with rhodamine fluorescein; and RP11-144H20 is cloned by specific BAC of the centromere of the chromosome 7 and marked with fluorescein isothiocyanate. The reagent can quickly, simply and conveniently detect the copy number of the EGFR gene and the ploidy of the chromosome 7, contributes to the judgment of medicament susceptibility and prognostic judgment, and has great significance for the guidance of use of molecular targeted medicament tyrosine kinase inhibitors (TKIs) of tumors, such as non-small cell lung cancer and the like.

A single base change in the Bn-FAE1.1 gene in the A genome and a two-base deletion in the Bn-FAE1.2 gene in the C genome produce the nearly zero content of erucic acid observed in canola. A BAC clone anchoring Bn-FAE1.1 from a B. rapa BAC library and a BAC clone anchoring Bn-FAE1.2 from a B. oleracea BAC library were used in this research. After sequencing the gene flanking regions, it was found that the dissimilarity of the flanking sequences of these two FAE1 homologs facilitated the design of genome specific primers that could amplify the corresponding genome in allotetraploid B. napus. The two-base deletion in the C genome gene was detected as a sequence characterized sequence region (SCAR) marker. To increase the throughput, one genome specific primer was labeled with four fluorescence dyes and combined with 20 different primers to produce PCR products with different fragment sizes. Eventually, a super pool of 80 samples was detected simultaneously, making it possible to analyze over half a million of samples per day using a medium capacity ABI 3100 Genetic Analyzer. This dramatically reduces the cost of marker detection. The single base change in the Bn-FAE1.1 gene was detected as single nucleotide polymorphic (SNP) marker with an ABI SNaPshot kit. A multiplexing primer set was designed by adding a polyT to the 5′ primer end to increase SNP detection throughput through sample pooling. These multiplexed high throughput molecular markers have been successfully implemented in our canola / rapeseed breeding programs.