White flower beet the 9th chromosome BAC chip and its preparation method
A technology of chromosome number and chromosome, which is applied in the field of BAC chip of white flower beet chromosome 9 and its preparation, and can solve the problems of density limitation of membrane chip, incomparability of signal value, etc.
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specific Embodiment approach 1
[0004] Specific embodiment 1: In this embodiment, the BAC clone on the BAC chip of chromosome 9 of the beet is to use the scattered repetitive sequence of the chromosome of the beet as the specific probe to screen the BAC DNA obtained from the BAC library. The aforementioned BAC DNA is the same as the carrier. It is obtained by compounding. In this embodiment, the 2377 BAC DNA obtained by screening the BAC library by using the scattered repetitive sequences of the chromosomes of white beet as a specific probe are all compounded with the carrier. The carrier is a solid phase carrier. The solid phase of this embodiment The carrier is a glass slide. Its preparation steps are: (1) BAC preliminary treatment: a. Dissolve 5-10μg of purified and extracted BAC DNA in 50μl of 0.1-0.15M NaOH solution, and in a water bath at 60-70℃ for 30-60 minutes B. Add 5μl of 0.1-0.2M glycidoxytrimethoxysilane dissolved in isopropanol; c. Water bath in a 60-70℃ water bath for 3-5 hours; e. Add 50μl of neu...
specific Embodiment approach 2
[0009] Specific implementation manner 2: Using the scattered repetitive sequences of the chromosomes of the beet as a specific probe to screen the BAC library, a part of the 2377 BAC DNAs are compounded with the vector.
specific Embodiment approach 3
[0010] Specific embodiment 3: The carrier in this embodiment is a silicon wafer, a nylon membrane, a nitrocellulose membrane or a gel.
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