The invention relates to a method for
tissue culture taking
lotus reproduction and rooting into account and belongs to the technical field of
plant tissue culture. The method comprises the steps of selecting full ripe seeds, taking mature embryos as explants, taking out the embryos after cleaning and sterilization, inoculating the embryos to a sterilized MS
solid culture medium with the pH of 5.7 and perform cultivation in a culture room with temperature of 25+ / -2 DEG C, photoperiod of 16 / 8 hours and
light intensity of 2400
lux. After the preliminary generation is cultivated for 4 weeks, aseptic seedlings with good growth conditions and free of
browning are transferred into a
reproduction culture medium to be cultivated, the MS culture medium is taken as a base culture medium by the
reproduction culture medium, 6-BA with concentration of 0.3-3 mg / L, NAA with concentration of 0.3-1 mg / L,
sucrose with concentration of 30 g / L and
agar of 0.3% are added, and the pH value is 5.7. According to the method, roots are induced while
bud reproduction can be performed quickly in the reproduction and cultivation process, so that a large amount of
lotus tissue culture seedlings are obtained, the time for expanding propagation is shortened, and the cost is reduced.