Method for cloning rice auxin induced protein gene

Abstract

The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.

Description

technical field [0001] The invention relates to a method for applying rice functional genomics, in particular to a method for cloning a rice auxin-induced protein gene. Background technique [0002] Rice is one of the three major food crops. More than one-third of the world's population uses it as the main source of food. It plays a pivotal role in the national economy. With the continuous increase of the world population and the increase of the demand for food, the annual demand for rice is constantly increasing. Today, with the shrinking land area, how to make rice production stable and high to alleviate the impact of the increase in population on rice production The pressure brought by it is a difficult problem faced by the world, and it is also a major scientific research topic in front of scientific researchers. Although traditional genetic research and conventional breeding have made important contributions to the improvement of rice yield and quality, due to the limi...

AI Technical Summary

Problems solved by technology

However, the serious lack of important functional genes with independent intellectual property rights is a "bottleneck" that seriously restricts the high-level development of agricultural biotechnology breeding in my country