The invention discloses a method for constructing an HSV-1 BAC (herpes simplex virus I bacterial artificial chromosome) system carrying luciferase report genes. The method comprises the following steps of: A, constructing a system, namely (1) designing primers according to HSV-1 F strain sequences, (2) constructing transfer vectors, (3) constructing recombinant viruses, (4) purifying plaques, (5) identifying the recombinant viruses, (6) constructing recombinant BAC, (7) saving the recombinant viruses and (8) measuring a growth curve; and B, constructing a Luciferase stable expression system, namely (1) designing amplified Luciferase-Hygr primers, (2) amplifying fragments by using high-fidelity KOD enzyme, (3) electrically transforming pHSV-1 BAC to E.coliDY380, (4) electrically transforming the reclaimed DNA fragments into pHSV-1 BAC/DY380 electrically-transformed competent cells, (5) identifying, (6) picking DY380 monoclone, (7) extracting BAC plasmids, (8) saving the viruses, and (9) performing half quantification on the viruses in vitro for the HSV-1 BAC Luc recombinant virus infected cells. The method is simple, feasible and convenient to operate, can modify the virus genomes in vitro, and also can perform half quantification on the virus particles in vitro.