93 results about "Bacterial artificial chromosome" patented technology

The invention is directed in part to totipotent cells that have one or more artificial chromosomes; processes for producing such cells; processes for using such cells (e.g., nuclear transfer); transgenic embryos and transgenic animals cloned from such cells; and processes for producing such embryos and animals.

The invention relates to a VAC-BAC shuttle vector system for creation of recombinant poxviruses from DNA cloned in a bacterial artificial chromosome.

The invention provides a method for screening DNA molecular markers closely correlative with properties, which is mainly used for identifying DNA molecular markers correlative with properties by combining super bulked segregant analysis (BSA) with a high throughput sequencing technique. The method comprises the following steps of: carrying out parameter training to data comprising different species genomes or BAC (Bacterial Artificial Chromosome) sequences and the like by utilizing a bioinformatics method to find out an optimal marker sequencing scheme for improving the development efficiency of the markers; carrying out correlative analysis to bulked segregant sequencing results to detect the molecular markers correlative with the properties and position back to the genomes or BAC, and then precisely positioning candidate functional genes through the high-density property-correlative molecular markers. Compared with the traditional method, the method has the advantages of greatly improving throughput and greatly reducing cost. The method can be used for carrying out marker positioning to large groups and directly determining the linkage between the markers and the target properties, has high reliability and accuracy of correlative analysis, and is mainly applied to the aspects of marker development and gene positioning, such as crop molecular breeding and the like.