29 results about "Genetic strain" patented technology

The invention provides an MsLEA3 gene and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the alfalfa MsLEA3 gene is subjected to overexpression or gene editing is carried out on the MsLEA3 gene in alfalfa through a CRISPR / Cas9 system, so that an overexpression transgenic line of the alfalfa MsLEA3 gene or a gene editing transgenic line aiming at the alfalfa MsLEA3 gene is obtained. The salt tolerance of an overexpressed or gene-edited medicago sativa transgenic line of the MsLEA3 gene is remarkably improved, the MsLEA3 gene can be used for cultivating medicago sativa varieties with high salt resistance, a new theoretical basis and candidate genes are provided for cultivating new varieties of high-resistance leguminous forage, and the application of medicago sativa in production is greatly promoted.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The application provides a kind of MsLEA3 Gene and its application belong to the technical field of plant genetic engineering.The application obtains the overexpression transgenic strain of alfalfa Medicago sativa L. MsLEA3 Gene or carries out gene editing to the gene of Medicago sativa L. MsLEA3 Through the overexpression of Medicago sativa L. MsLEA3 Gene or the gene editing transgenic strain of Medicago sativa L. MsLEA3 Gene.The overexpression or gene editing transgenic strain of Medicago sativa L. MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLEA3 MsLE Gene significantly improves the salt tolerance of the transgenic strain, can be used for cultivating Medicago sativa L.varieties with high salt resistance, provides a new theoretical basis and candidate gene for cultivating new varieties of legume forage grass with high resistance, and greatly promotes the application of Medicago sativa L.in production.

The invention discloses application of a sorghum SbPGK1 gene in improving plant disease resistance, and belongs to the technical field of biology. The nucleotide sequence of the sorghum SbPGK1 gene is as shown in SEQ ID NO.9. By analyzing phenotypes and related physiological indexes of SbPGK1 rice transgenic lines before and after inoculation of different pathogenic bacteria for infection and sorghum, the results show that compared with non-SbPGK1 gene rice lines, the SbPGK1 transgenic lines have obviously better resistance to magnaporthe oryzae and bacterial blight germs than wild types, silence SbPGK1 in sorghum has better resistance to rice blast germs and bacterial blight germs, and the SbPGK1 gene has better resistance to rice blast germs and bacterial blight germs. The SbPGK1 gene has the advantages that the SbPGK1 silent strain has reduced disease resistance to colletotrichum gloeosporioides, the SbPGK1 is overexpressed in sorghum, and the SbPGK1 overexpressed strain has enhanced disease resistance to colletotrichum gloeosporioides, so that the SbPGK1 gene is a potential disease-resistant breeding gene and can be used for plant disease-resistant genetic improvement.

The invention belongs to the technical field of molecular biotechnology and plant genetic engineering, and particularly relates to a grape MYB transcription factor VvMYB108 and application thereof. The invention provides an MYB transcription factor VvMYB108 for regulating and controlling biological stress in a postharvest storage process of grapes. An overexpression strain of the VvMYB108 in arabidopsis thaliana is utilized to find that the sensitivity of a VvMYB108 transgenic strain to botrytis cinerea is enhanced. By using an overexpression strain of the VvMYB108 in arabidopsis thaliana, it is found that the disease resistance of the VvMYB108 transgenic strain to Pst DC3000 is enhanced.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.

The invention discloses an anti-clubroot gene BolC6. MYB122 and application thereof, and relates to the technical field of plant biology. The NDA sequence of the BolC6. MYB122 provided by the invention is as shown in SEQ ID NO. 1, and the CDS sequence of the BolC6. MYB122 provided by the invention is as shown in SEQ ID NO. 2. A constructed 35S: BolC6. MYB122-eGFP overexpression vector is transformed into wild type arabidopsis thaliana, an arabidopsis thaliana myb122 mutant is obtained, namely an overexpression transgenic line is obtained, the resistance of overexpression positive regulation of the BolC6. MYB122 gene to plasmodiophora brassicae is clarified through disease resistance identification, and the transgenic line is a transgenic line. And a theoretical basis and gene resources are provided for clubroot-resistant molecular breeding of plants such as brassica napus.

The present invention provides a method for detecting the insertion copy number of foreign genes in Setaria viridis or foxtail millet by fluorescence quantitative PCR or digital PCR, belonging to the field of biotechnology. The method uses RA1 gene as an internal reference gene and Hyg screening marker gene as a foreign gene, and uses primer pairs RA1-F / RA1-R and primer pairs Hyg-F / Hyg-R to detect the genomic DNA of transgenic Setaria viridis or transgenic foxtail millet, and determine the insertion copy number of foreign genes in transgenic Setaria viridis or transgenic foxtail millet. The present invention provides a method for detecting the insertion copy number of foreign genes in Setaria viridis or foxtail millet by fluorescence quantitative PCR or digital PCR, which has the characteristics of simplicity, rapidity, low sample consumption, high efficiency, high throughput, etc., can improve the efficiency of screening transgenic lines of Setaria viridis or foxtail millet with low-copy insertions, and provides a new option for the detection method of the insertion copy number of foreign genes in the research of transgenic Setaria viridis or transgenic foxtail millet.

The present invention discloses the application of the OsRbohI gene in regulating rice disease resistance, belonging to the field of biotechnology. The present invention verified multiple OsRbohI mutants with different genetic backgrounds and found that they almost did not produce reactive oxygen species bursts induced by plant immune elicitors, and their resistance to the most destructive rice blast fungus was significantly weakened; Arabidopsis transgenic strains were obtained using the floral dipping transformation method. The OsRbohI protein driven by the Arabidopsis AtRbohD promoter can cross-species complement the reactive oxygen species burst phenotype of the dicotyledonous model plant atrbohd mutant, indicating that OsRbohI can play a cross-species role. The present invention proves the important role of OsRbohI in rice disease resistance, provides new gene resources for rice disease resistance breeding, and lays a foundation for the selection of disease-resistant varieties for other species.

The application discloses a kind of auxin response SAUR gene family protein genes and purposes thereof, and belongs to the technical field of genetic engineering.The application first finds that the expression pattern of auxin response SAUR gene family protein gene HvSAUR36 is significantly positively correlated with the accumulation of valine, leucine and isoleucine.The application constructs HvSAUR36 overexpression vector and transforms tobacco, and detection shows that the content of valine, leucine and isoleucine in the leaves of transgenic lines is significantly higher than that of wild type.It is proved that the auxin response SAUR gene family protein encoded by HvSAUR36 plays a key catalytic role in the biosynthesis pathway of plant branched-chain amino acids, and provides an important target gene for crop quality improvement.The gene, its recombinant vector, recombinant bacteria and transgenic plants all have good application prospects.

The invention discloses an auxin response SAUR gene family protein gene and application thereof, and belongs to the technical field of gene engineering. The invention discovers that the expression mode of the auxin response SAUR gene family protein gene HvSAUR36 is obviously positively correlated with the accumulation of valine, leucine and isoleucine for the first time. According to the invention, an HvSAUR36 overexpression vector is constructed and is used for transforming tobacco, and detection shows that the valine content, the leucine content and the isoleucine content in leaves of a transgenic line are obviously improved compared with those of a wild type. The HvSAUR36-coded auxin response SAUR gene family protein is proved to play a key catalytic role in a plant branched chain amino acid biosynthetic pathway, an important target gene is provided for crop quality improvement, and the gene and a recombinant vector, a recombinant bacterium and a transgenic plant thereof have good application prospects.

The application belongs to the field of plant genetic engineering and biotechnology, and particularly relates to application of CmEXPB1 gene of melon in improving fruit resistance to cracking. The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The gene CmEXPB1 is cloned from melon cDNA, and it is found by real-time fluorescent quantitative technology that the expression amount of the gene in the peel of melon resistant to cracking is significantly higher than that in the peel of melon susceptible to cracking. A plant expression vector is constructed, and is transformed into tomato, and it is found that the peel hardness, cortex layer toughness and tightness of the transgenic strain are significantly higher than those of the wild type, and the brittleness is significantly reduced; cell wall component determination finds that the contents of cellulose, hemicellulose and protopectin of the transgenic tomato are increased, and the contents of soluble pectin and lignin are reduced, thereby confirming that CmEXPB1 enhances the resistance to cracking by regulating the composition of cell wall. The application lays a theoretical foundation for studying the fruit resistance to cracking, and also provides a gene resource for cultivating horticultural plants with high resistance to cracking.

This invention belongs to the field of gene breeding technology, specifically relating to the application of the cascade signal kinase OsMAPKKK5 in improving rice resistance to bacterial blight. This invention provides the protein OsMAPKKK5 or substances regulating the activity and / or content of said protein, and the application of related biological materials. This invention constructs... OsMAPKKK5 Transgenic lines overexpressing the pathogen were inoculated with bacterial blight pathogens in the field for resistance phenotypic analysis, and the results showed that... OsMAPKKK5 Compared to wild-type rice varieties, the overexpressed transgenic lines showed shorter lesions and exhibited a resistance phenotype against bacterial blight, thus demonstrating improved immunity. OsMAPKKK5 The expression level of this gene can promote the resistance to bacterial blight in rice. This invention provides important genetic resources and molecular theoretical basis for the development of new rice varieties resistant to bacterial blight.

The application discloses a Pinus massoniana tandem zinc finger structure PmTZF1 gene and an expression protein and application thereof, and relates to the technical field of plant genetic engineering.The Pinus massoniana tandem zinc finger structure PmTZF1 gene is disclosed for the first time, a nucleotide sequence of which is shown as SEQ ID NO.1, and an amino acid sequence of which is shown as SEQ ID NO.2.The application constructs an expression vector pBI121-PmTZF1 of the Pinus massoniana tandem zinc finger structure PmTZF1 gene; the constructed expression vector of the Pinus massoniana tandem zinc finger structure PmTZF1 gene is transformed into Arabidopsis; and a transgenic Arabidopsis plant with improved non-biological stress resistance is obtained through cultivation and screening.Compared with a wild type plant, the root of the transgenic strain is significantly longer than that of the wild type, and the transgenic strain blooms and sprouts earlier; under drought stress, the transgenic strain normally blooms and bears fruits, has more branches, and has more silique quantity, length and other properties, which are significantly better than those of the wild type strain.

The application discloses a ginkgo biloba L. GbC4H1 gene, a coding protein and application thereof, and relates to the technical field of plant genetic engineering.The ginkgo biloba L. GbC4H1 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the coding protein is as shown in SEQ ID NO.2.The expression vector of the ginkgo biloba L. GbC4H1 gene is constructed and transformed into Nicotiana benthamiana; and a transgenic plant with increased flavonoid metabolite content is obtained through cultivation and screening.Compared with a control group, the expression level of a transcription factor of a flavone synthesis pathway related gene and a flavone synthesis pathway structural gene in the transgenic strain is significantly increased.The flavonoid metabolite content in the transgenic strain is increased, and compared with the control group, 14 kinds of differential metabolites are up-regulated and 20 kinds of differential metabolites are down-regulated.

The invention discloses a BnaC06.ATF2 protein for promoting flowering of brassica napus as well as a coding gene and application thereof, and relates to the technical field of biology. According to the invention, 51 HAT family members are identified in brassica napus, wherein the expression level of the BnaC06. ATF2 gene in floral organs is high. By constructing an overexpression transgenic line of the BnaC06. ATF2 and analyzing the flowering phenotype of the overexpression transgenic line, the invention finds that the flowering time of the overexpression line of the BnaC06. ATF2 gene is obviously advanced compared with that of a control plant (ZS11). Transcriptomics data reveals that overexpression of the BnaC06. ATF2 causes change of expression levels of flowering regulation genes and hormone signal pathway related genes, so that flowering of the brassica napus is promoted. The invention provides important technical support for obtaining the early flowering phenotype brassica napus germplasm by utilizing a genetic method.

The invention provides a function of a BnaFLP2 gene for regulating and controlling thousand seed weight of rape seeds and application of the BnaFLP2 gene, and belongs to the technical field of gene engineering. The invention provides a BnaFLP2 gene for regulating and controlling the thousand seed weight of rape seeds, BnaC9.FLP2c is used as a target gene, and a complementary and over-expressed BnaC9.FLP2c transgenic line and a mutant material of which the gene is knocked out are constructed. By investigating field traits, it is found that in a complementary BnaC9.FLP2c transgenic positive material and an overexpression plant, the expression quantity of BnaC9.FLP2c is remarkably increased, but the thousand seed weight is remarkably reduced, the plant height, the number of branches and the like are not affected, and it is proved that BnaC9.FLP2c negatively regulates the thousand seed weight of Brassica napus and basically does not affect other yield-related agronomic traits.

The application belongs to the field of plant disease prevention and treatment, and particularly relates to application of a rice OsSRR1 gene in resistance to rice stripe disease. The application screens a new target gene OsSRR1 for preventing and treating rice stripe disease through transcriptome sequencing analysis, qRT-PCR verification and observation of disease resistance phenotypes of rice transgenic lines infected with rice stripe virus. After mutation of the gene, the content of rice stripe virus in the rice can be significantly reduced, and the formation of disease spots can be inhibited. In summary, the application provides use of the OsSRR1 gene and the mutant thereof in genetic improvement and breeding of rice disease resistance, and has great application prospect.

The invention discloses an MsPAL3 protein for improving resistance of valsa mali, a gene and application of the MsPAL3 protein and the gene, and belongs to the technical field of gene engineering. The amino acid sequence of the MsPAL3 protein is as shown in SEQ ID NO. 2, and the nucleotide sequence of the MsPAL3 gene is as shown in SEQ ID NO. 1. According to the invention, the valsa mali resistant gene MsPAL3 is identified, and the resistance of valsa mali can be remarkably enhanced by overexpressing the MsPAL3 gene. The analysis of phenotypes of MsPAL3 transgenic lines before and after pathogen infection shows that the MsPAL3 overexpression transgenic lines have obvious valsa ceratosperma resistance characteristics for non-transgenic lines, so that the MsPAL3 gene is a potential valsa ceratosperma resistance gene, and a new gene resource and a technical path are provided for cultivating disease-resistant apple varieties.

The present invention discloses a S-adenosyl-L-methionine δ24-sterol-C-methyltransferase mutant C24MTgm-M11. Strain MG1655 (ΔptsG ΔfumAC ΔfumB, panD, aspA, C24MTgm) is constructed based on the polynucleotide encoding the enzyme mutant. Strain MG1655 (Δpts GΔfumAC ΔfumB, panD, aspA, C24MTgm-M11) can produce 480 mg / L 3-aminoisobutyric acid under shake flask fermentation. Compared to the wild type strain C24MTgm, the strain containing mutant C24MTgm-M11 has a significantly improved ability to produce 5.8 times' 3-aminobutyric acid.

This invention discloses the LhCOLD1 gene of hybrid tulip tree, its expressed protein, and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the LhCOLD1 gene disclosed in this invention is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention constructs an overexpression vector of the LhCOLD1 gene of hybrid tulip tree and transforms it into callus tissues of Arabidopsis thaliana and hybrid tulip tree; transgenic lines with enhanced resistance to low-temperature stress are cultivated, screened, and obtained. After being treated at -10℃ for 3 hours and then allowed to recover at room temperature for two weeks, the growth of LhCOLD1 transgenic Arabidopsis thaliana plants was less affected by low-temperature stress; the growth of LhCOLD1 transgenic hybrid tulip tree lines was less affected by low-temperature stress after being treated at -10℃ for 1.5 hours. The results indicate that the LhCOLD1 gene enhances the plant's tolerance to low-temperature stress.

The invention relates to a synthetic immunoglobulin dynamic locus mouse model and a preparation method and application thereof.The synthetic immunoglobulin dynamic locus comprises (V cluster)-(D cluster)-Rc-(J cluster)-IGHM and a large number of VDJ functional genes according to the principle of generic-genome design and modular locus design, a controllable recombination system is introduced, and the synthetic immunoglobulin dynamic locus is obtained. The diversity and the specificity of the antibody are obviously improved. The method comprises the following steps: integrating designed and constructed synthetic immunoglobulin dynamic loci into a mouse genome through transposase, and constructing a stable genetic strain to obtain a model mouse; the model mouse has outstanding antibody diversity and a dynamic recombination effect, and a novel tool can be provided for antibody drug research and development and immunological research.

The present invention provides a method for detecting the copy number of exogenous gene insertion in Setaria viridis or foxtail millet by digital PCR, belonging to the field of biotechnology. The method uses the Sevir.3G057200 gene as an internal reference gene and Hyg the selectable marker gene as the exogenous gene, and uses the primer pairs 200-F / 200-R and Hyg-F / Hyg-R to detect the genomic DNA of transgenic Setaria viridis or transgenic foxtail millet, and to determine the copy number of exogenous gene insertion in transgenic Setaria viridis or transgenic foxtail millet. The method of the present invention has the characteristics of being simple, rapid, low sample consumption, efficient, and high-throughput, can improve the efficiency of screening transgenic lines of Setaria viridis or foxtail millet with low-copy insertion, and provides a new option for the detection method of the copy number of exogenous gene insertion in the research of transgenic Setaria viridis or transgenic foxtail millet.

The invention provides an application of a PaBBX30 protein and a coding gene thereof in regulating dormancy delay of a plant terminal bud, and belongs to the technical field of plant genetic engineering. The amino acid sequence of the protein PaBBX30 is as shown in SEQ ID No. 2. The method comprises the following steps: connecting a gene for coding the PaBBX30 protein to a p2301s vector by a homologous recombination method to construct an over-expression vector; the overexpression vector is introduced into a plant by adopting an agrobacterium-mediated transformation method, a positive transgenic line is screened out through kanamycin, and whether the PaBBX30 gene in the transgenic line is expressed or not is detected; the overexpression strain is subjected to short-day treatment, the dormancy performance of the overexpression strain is observed, and the terminal bud dormancy of the overexpression PaBBX30 gene strain is obviously delayed. The method is suitable for being applied to growth rhythm regulation and breeding improvement technologies of landscaping plants, fruit trees or other perennial plants.

The application discloses a camellia nitidissima gene CpPAL and application thereof in regulating flavonoid content in plant leaves, wherein the nucleotide sequence of the camellia nitidissima gene CpPAL is shown as SEQ ID NO. 1. The application preliminarily predicts the function of the gene based on previous research and bioinformatics analysis software, obtains the full length of the gene sequence through cloning, and constructs an overexpression vector and performs transient transformation on tobacco on this basis. The results show that the flavonoid content in the leaves of the CpPAL transgenic strain is obviously higher than that of the empty carrier. As the gene CpPAL for regulating the flavonoid content in the leaves of camellia nitidissima, the application can be applied to some plants with poor solarization resistance in genetic engineering, and has practical application value.

The invention discloses an MsWRKY61 protein for improving resistance of valsa mali, a gene and application of the MsWRKY61 protein and the gene, and belongs to the technical field of gene engineering. The amino acid sequence of the MsWRKY61 protein is as shown in SEQ ID NO. 2, and the nucleotide sequence of the MsWRKY61 gene is as shown in SEQ ID NO. 1. According to the invention, the valsa mali resistant gene MsWRKY61 is identified, and the resistance of valsa mali can be remarkably enhanced by overexpressing the MsWRKY61 gene. According to the application disclosed by the invention, phenotypes of MsWRKY61 transgenic lines before and after germ infection treatment are analyzed to show that the MsWRKY61 overexpression transgenic lines have obvious valsa ceratosperma resistance characteristics for non-transgenic lines, so that the MsWRKY61 gene is a potential valsa ceratosperma resistance gene, and a new gene resource and a technical path are provided for cultivating disease-resistant apple varieties.

The invention belongs to the field of plant disease control, and particularly relates to application of a rice OsSRR1 gene in resisting rice stripe disease. The novel target gene OsSRR1 for preventing and treating the rice stripe disease is screened by carrying out transcriptome sequencing analysis on rice infected with the rice stripe virus, carrying out qRT-PCR (quantitative reverse transcription-polymerase chain reaction) verification and observing the disease-resistant phenotype of a rice transgenic line. After the gene is mutated, the content of the rice stripe virus in the rice can be obviously reduced, and the formation of disease spots is inhibited. In a word, the invention provides the application of the OsSRR1 gene and the mutant thereof in genetic improvement breeding in rice disease resistance, and the application prospect is huge.

The invention relates to a rice heading period gene Hd20 and application thereof, and belongs to the field of gene engineering. The invention provides a rice heading period gene Hd20, the nucleotide sequence of which is as shown in SEQ ID No.2, and the rice heading period gene Hd20 is used for coding a vesicle sorting protein containing an FYVE-PTB structural domain. The method can be used for directionally breeding early / late heading varieties; the variety adaptability is improved. The method is accurate and efficient, stable genetic strains can be obtained only through 1-2 generations, and the limitation of traditional hybridization and backcross is broken through. Yield and quality are increased, climate adaptability is achieved, accumulated temperature is fully utilized for late heading varieties, temperature and light resource waste caused by global warming is relieved, early heading varieties adapt to short growing season areas, and drought / high temperature stress risks are reduced.