Method for Breeding Brassica napus Varieties and Rapeseed Rapeseed Double Haploid Inducer Lines
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A mustard-type rape and double haploid technology, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of low induction efficiency, achieve high induction efficiency, improve breeding efficiency, and save breeding time Effect
Active Publication Date: 2018-05-25
CHENGDU ACAD OF AGRI & FORESTRY SCI
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The earliest maize haploid induction line is stock6, which can only induce haploids in maize, and then the haploid plants undergo artificial chromosome doubling to form homozygous diploids (double haploids), and the induction efficiency Low, generally the induction efficiency is below 10% (calculated based on the number of haploids obtained from harvested seeds)
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Embodiment 1
[0080] see figure 1 , figure 2 , Figure 5 , Figure 7 , mustard-type rape dogtail GW (a local variety in Sichuan mountainous areas, tetraploid, 2n=36) has the characteristics of yellow seeds, disease resistance, lodging resistance, high oil content (45%), compact plants, and poor soil tolerance. High (more than 2.5 meters) and late maturity; another mustard-type rapeseed broom mustard SBJ (Chengdu Jintang local variety, tetraploid, 2n=36) has short growth period, early maturity, many branches, and short plant height ( About 150cm), resistant to poor soil and other characteristics, but the content rate is low (35-38%), brown seeds, slender, fluffy and not compact. In order to breed better plant type, moderate plant height (160-170cm), yellow seeds, short growth period, early maturity, high oil content, and resistance to poor soil, the above two landraces were emasculated and crossed, F 1 Bud stripping and emasculation at the generation flowering stage, the double haploid...
Embodiment 2
[0159] see figure 1 , figure 2 , Figure 4 , Figure 8 , purple mustard type rape ZJ, purple leaves (with a strong ornamental), but long growth period, plant height of about 240CM, brown seed coat, production in addition to poor ornamental yield traits. Mustard-type rape mustard SBJ (Chengdu Jintang local variety, tetraploid, 2n=36) has the characteristics of short growth period, early maturity, many branches, short plant height (about 150cm), and tolerance to poor soil. Agronomic traits. In order to breed better plant type, moderate plant height (160-180cm), short growth period, early maturity, tolerance to poor soil and purple leaf mustard rapeseed, the above two landraces were emasculated and crossed, F 1 Bud stripping and emasculation at the generation flowering stage, and the rapeseed double haploid induction line Y3560 obtained by the applicant was used as the male parent for pollination induction, F 2 The generation (induced offspring) was planted as a single pla...
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Abstract
The invention discloses a method for breeding leaf mustard type rape varieties and materials by a rape double haploid inducing line. The method comprises the following steps: firstly, determining objective traits of the bred leaf mustard type rape varieties and materials; secondly, performing hybridization, convergent cross or backcross on at least two leaf mustard type rapes with the objective traits; thirdly, pollinating progenies obtained by hybridization, convergent cross or backcross by using the rape double haploid inducing line; fourthly, identifying the genetic stability of induced progenies, and realizing selfing breeding according to strains; fifthly, identifying the yield, resistance and quality traits of induced stable progeny strains; sixthly, determining the stable strains according to the yield, the quality traits and the resistance to form a conventional variety of leaf mustard type rape; seventhly, inducing stable progeny genetic strains to form a new leaf mustard type rape line and performing test cross on the new leaf mustard type rape line and a leaf mustard type rape sterile line to form a maintainer line or restorer line, or entering next round of breeding process of the varieties and materials. According to the method, the breeding speed and efficiency of a hybrid variety or a conventional variety of the leaf mustard type rape can be greatly improved, and labor force and material resources are reduced.
Description
Technical field: [0001] The invention is related to agriculture, in particular to a method for breeding mustard-type rape varieties and materials with rapeseed double haploid induction lines. Background technique: [0002] Rapeseed is the main oil crop in my country, including 3 cultivars, Brassica napus ( Brassica napus , a compound species evolved from Brassica oleracea (aa, n=10) and Brassica cabbage (cc, n=9) through natural interspecific hybridization and double diploidization. According to the source of chromosomes, it is tetraploid, 2n=38 ); Brassica napus ( Brassia campestris L. Including Brassica and Chinese cabbage native to China. China is also known as Chinese cabbage, dwarf rape, sweet rape and so on. The chromosome group is aa, n=10, and it is judged to be diploid according to the source chromosome group source, 2n=20,); Brassica juncea, composed of Brassica juncea (aa, n=10) and black mustard (bb, n=8) A compound species evolved from double diploidization...
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