54results about How to "Growth is not affected" patented technology

The invention relates to a pelleting coating method for Trifolium repens seeds and creates conditions for mechanization of grassland construction by carrying out pill coating on the Trifolium repens seeds. The method comprises the following steps: (1) processing of the Trifolium repens seeds: prior to the pill coating, the Trifolium repens seeds are processed by air separation and screening; (2) preparation of a coating agent: the coating agent comprises an A type coating agent, a B type coating agent, a C type coating agent, adhesive and nutrient fluid; (3) process of pill coating of the Trifolium repens seeds: the Trifolium repens seeds processed by the procedures are put in a coating machine, the external surfaces of the seeds are covered with the adhesive evenly, and the A type coating agent is added; then, the B type coating agent is added, and simultaneously liquid jetted by a flow feeder is changed into the nutrient fluid; liquid jetting is carried out continuously, and coating powder is added for pelleting; after the B type coating agent is added, the adhesive and the C type coating agent are added; and after being taken out, the seeds are insolated or dried in time, and after the insolation or drying, the coated seeds are packaged by fiber drums.

The invention belongs to the technical field of plant genetic engineering, and specifically designs a carrier for improving the regeneration efficiency of strawberry leaves and application of the carrier. An RNA interference fragments of a forest strawberry cytokinin receptor gene FveHK2 is screened from a forest strawberry, a plant inhibition expression vector GN2300-FveHK2 of the forest strawberry cytokinin receptor gene FveHK2 is constructed, then transfection is performed on transgenic engineering bacteria, the plant inhibition expression vector GN2300-FveHK2 is transferred into a diploidforest strawberry 'Hawaii 4' to obtain an RNAi expression interference strain line of the FveHK2 gene, under the condition of in-vitro leaf tissue culture, the germination time of the FveHK2 gene inhibition expression line is earlier than that of a wild type, and the budding rate of the FveHK2 gene inhibition expression line is significantly higher than that of the wild type, which indicates thatthe regeneration ability of the leaves of the forest strawberry transgenic line with the genetic transformation FveHK2 interference vector is significantly improved. The carrier for improving the regeneration efficiency of the strawberry leaves has important significance for the regeneration of strawberry leaves and the improvement of the transformation efficiency.

The invention discloses a crab-rice symbiotic planting-breeding pool and a comprehensive temperature-adjusting method thereof. By using the crab-rice symbiotic planting-breeding pool, symbiosis and coculture of river crabs and high-straw rice can be realized; the temperature of water in the crab-rice symbiotic planting-breeding pool in hot summer can be controlled below 28 DEG C, so normal feeding and molting of the river crabs in summer can be guaranteed; ventilation, air permeation and enough illumination can be obtained among the high-straw rice; pests parasitized on rice straws can be feeds for aquatic animals like the river crabs and wild fishes; the use of a pesticide is not needed; manures excreted by the aquatic animals like the river crabs and the wild fishes are used as an organic fertilizer of the high-straw rice, so benign ecological balance is realized in the crab-rice symbiotic planting-breeding pool; farmers can simultaneously obtain high-quality organic rice with high yield and large-sized river crabs with high yield; the comprehensive productivity of a crab pool is improved; the product quality is enhanced; and the economic benefits are increased.

The invention discloses a method for rapid tissue propagation of acer saccharum, and belongs to the technical field of woody plant tissue culture and propagation. The method comprises the following steps: selecting explants, pretreating the explants, establishing a sterile system, performing inductive culture, performing multiplication culture, performing rooting culture, strengthening and domesticating seedlings, and the like. Tissue organs of plants are cultured under a sterile system condition by controlling external environment conditions artificially, the purpose of rapidly propagating nursery stock is achieved, and stable properties of excellent female parents are effectively maintained. Compared with common vegetative propagation of cuttage and grafting, the method is small in material amount, free of limit of natural conditions and relatively applicable to neophytes, the propagation coefficient is as high as 5-20 times, and propagation and popularization problems caused by shortage of resources of the acer saccharum are solved.

The invention provides a pig feed and a preparation method thereof. The pig feed comprises 1 percent by weight of premix, a synthetic amino acid and a compound enzyme, wherein the synthetic amino acid is a mixture of L-lysine, DL-methionine, L-threonine and L-tryptophan; the compound enzyme is a mixture of protease, amylase, phytase, xylanase and beta-mannase. Compared with a common pig feed, the daily pig feed prepared from the synthetic amino acid and the compound enzyme has the advantages that the daily grain and the conversion efficiency of the feed are obviously increased, and fecal nitrogen and urine nitrogen are obviously reduced; the growth speed of pigs is increased, and the environmental improvement is facilitated; the growth of animals is not affected by the added synthetic amino acid; the reduction of the crude proteins of the daily pig feed facilitates the development of intestinal tracts, the villus height of duodenum in the intestinal tracts is increased, and the digestion and the absorption of proteins are facilitated.

The invention discloses a method for planting rice by fresh water irrigation and drainage and salt control in heavy saline-alkali land. The method comprises the following steps: 1, leveling cultivatedland with high precision; 2, preparing a water feeding and drainage system; 3, deeply loosening soil, wherein deep loosening is carried out by using a soil loosening plough, the depth is more than 60cm; 4, executing drip washing with water, wherein drip washing with water is that firstly ploughing is executed, then irrigation and soaking for 3 days is executed, and then quick water drainage is executed, and one or more times of drip washing with water is adopted according to the drip washing effect; 5, planting rice; 6, executing normal irrigation and drainage of fresh water, wherein fresh water is normally irrigated and drained at the initial stage of rice planting so as to achieve the aim of salt washing and controlling; 7, lightly baking fields and adopting no field baking; 8, improving the water quality requirement during the heading and flowering periods of the rice; 9, delaying water cut-off time, wherein water in the fields can be drained thoroughly after grain filling of therice and a yellow ripening stage comes. By adopting the method, rice can be planted in the heavy saline-alkali land, and it is proved through the practice that the growth of the rice is not affected.

The invention relates to the technical field of plant planting and cultivating, in particular to a planting method for wormwood. The planting method comprises the following steps of film mulching and soil preparation, ploughing and seedling planting, field management, seedling harvest and plant dividing; thin film is placed at the bottoms of field ridges during film mulching and soil preparation, during plant dividing and seed overturning, the thin film is shaken to loosen soil on the upper portions of the field ridges, and then wormwood roots are selected out directly. According to the planting method of the wormwood, the thin film can reduce the loss of water on the upper portions of the field ridges, pests and diseases of the wormwood roots are reduced, and the damage to the wormwood roots in plant dividing and seed overturning can be prevented.

Provided are a straw deep burying and returning method and device. The method includes the following steps of firstly, conducting picking and pulverizing through a deep burying and returning device; secondly, breaking original ridges into furrows through a ditching plow in the device after picking and pulverizing are completed, meanwhile making a deep burying plow in the device go deep into furrowbottom layers, and scattering pulverized straw discharged from a feeding box into a plow cavity of the deep burying plow onto the furrow bottoms through a deep burying conveyer so that the deep burying of the straw can be completed and the formed furrows can serve as the furrows for the tillage in the next season; thirdly, making loose soil on the upper side and the two sides intermediately falland converge to evenly bury the straw scattered on the furrow bottoms when the deep burying plow passes through the soil in the furrow bottoms, and combining original furrows into ridges as the foundation parts for the plantation in the next season. By means of the method and device, the alternate straw pulverizing and deep burying operation is conducted, the problems in an existing straw pulverizing and burying technology are successfully solved, and the straw returning method is efficient.

The invention discloses a special planting frame for flowers. The special planting frame includes a supporting rod and a fixing device. A rod body of the supporting rod is provided with four vertically formed grooves, and supporting legs are mounted at the lower ends of the inner walls of the grooves through rotating shafts. The supporting rod is sleeved with a first sleeve, and the upper end of the supporting rod is connected with a movable rod. During use of the special planting frame, first the supporting legs are unfolded, the first sleeve is rotated to make the lower end of the first sleeve come into contact with the supporting leg, at the time the supporting rod can be stably supported, the fixing device is mounted on the supporting rod or the movable rod by using a second sleeve, atthe time a check ring inside a fixing ring can clamp flowerpots of different diameters under the elastic action, and the operation process is convenient and simple.

The invention provides a device applicable to mycorrhizal seedling cultivation under an open-air condition, belonging to the technical field of cultivation of plant seedlings. The device is composed of a container body, a light-shielding, rain-blocking and dust-preventing cover and a water guiding unit. According to the invention, an aseptic seedling is firstly cultivated by utilizing the container body designed by the invention; a microbial inoculum is introduced to the root system of the aseptic seedling from the positions of inoculation holes of the container body; through arrangement of the water guiding unit and wrapping with the light-shielding, rain-blocking and dust-preventing cover, the container body is moved to the outside; thus, cultivation of a mycorrhizal seedling under the open-air condition is realized. The device provided by the invention can realize anti-pollution cultivation of the mycorrhizal seedling without application of greenhouse facilities or indoor conditions, i.e., under the open-air condition, solves a series of technical problem of large-scale, low-cost and high-quality cultivation of the mycorrhizal seedling, can also be used for nursery seedling raising in an arid region, can greatly save consumption of water, and improves the survival rate of a seedling.

The invention provides a method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi (clustered regularly interspaced short palindromic repeats i) regulation. According to the method, an acetaldehyde dehydrogenase coding gene of the corynebacterium glutamicum is replaced through a dCas9 expression cassette gene; a lactic dehydrogenase encoding gene of corynebacterium glutamicum is replaced by sgRNA transcription units of an isocitrate dehydrogenase encoding gene and a malic acid synthetase encoding gene, an isocitrate lyase encoding gene of the corynebacterium glutamicum is over-expressed to obtain a corynebacterium glutamicum engineering strain, and the engineering strain is cultured to ferment and synthesize glyoxylic acid. Corynebacterium glutamicum is used as a starting strain, a lactic dehydrogenase coding gene and an acetaldehyde dehydrogenase coding gene which are key enzymes of branch metabolism of the corynebacterium glutamicum are knocked out, a CRISPRi regulation system is established, expression levels of an isocitrate dehydrogenase coding gene and a malic acid synthase coding gene are reduced, and meanwhile, an isocitrate lyase coding gene is overexpressed, so that a method for biosynthesizing glyoxylic acid by using corynebacterium glutamicum is established.

The invention relates to a multi-stage purification ecological wave-eliminating system for a high stormy wave and low transparency water body area, and particularly relates to the technical field of water environment ecological repairing. The system comprises an ecological submerged dike, the ecological submerged dike comprises a submerged dike body and benthonic organisms, wherein the benthonic organisms are put on the submerged dike body; the submerged dike comprises a first submerged dike and a plurality of second submerged dikes, and the first submerged dike is arranged at a preset distance from the shore side in the length direction along the shore side; the secondary dikes are connected with the first submerged dike at intervals and extended in the lake center direction; submerged plants are included, and the submerged plants are planted at the position between the shore side and the first submerged dike; floating leaved plants are further included, and the floating leaved plantsare planted at the lake gaps between the first submerged dike and the second submerged dikes. According to the system, the system has a wave-eliminating effect, and water bodies can be purified.

The invention discloses an FLP/FRT gene knockout method of Candida amazonensis and belongs to the technical field of gene engineering. The FLP/FRT gene knockout method comprises the following steps: putting an FLP recombinase gene under a strictly induced type promoter SpGAL1p or SpMAL1p and regulating and controlling; adding directly repeated FPT sites to two ends of an FLP expression box and a resistance marker expression box; constructing a gene knockout box by taking PDC as a target gene and integrating the gene knockout box into a Candida amazonensis genome; then, adding an induction agent to induce FLP expression, so as to realize excision of resistance genes. According to the FLP/FRT gene knockout method disclosed by the invention, marker-free gene knockout of the Candida amazonensis is realized for the first time, and the method can be used for continuously knocking out a plurality of genes; a powerful technical means is provided for modification of metabolism engineering of the Candida amazonensis.

The invention belongs to the agriculture plantation technical field, and especially relates to a sunflower interplanting spring wheat plantation method before sowing; a conventional planting mode has two crop symbiosis stage short problems; the method wholly uses a base fertilizer, separates plots, and plants crops in sections, thus improving the sunflower and wheat production yield and quality; the method can fully utilize sunlight, heat and soil resources, can realize one-season-two-cropping, can reduce land surface exposure, can match quality seeds with fine method, can combine farm machinery with agronomic process, thus fully increasing output, quality and benefits, increasing yield and incomes, and protecting farmland ecology environment; the method is strong in practicality and wide in application prospects.

The invention provides a method for cultivating a Lactarius akahatsu mycorrhizal seedling. The method is characterized by comprising the following steps: 1) preparation of a seedling cultivation device: 2) cultivation of an aseptic seedling; 3) preparation of a microbial inoculum; 4) inoculation; 5) addition of a seedling cultivation substrate; 6) arrangement of a water guiding device and a light-shielding, rain-blocking and dust-preventing cover; and 7) cultivation of a mycorrhizal seedling. By adoption of the device designed by the invention for cultivation of the Lactarius akahatsu mycorrhizal seedling, the infection rate of miscellaneous bacteria is low, and the infection rate of puffball is zero.

A glass bottle plant decoration plastic rack can enhance root survival conditions under a conventional net condition, so the plant can be formed and viewed without affecting basic plant growth; the glass bottle plant decoration plastic rack is suitable for various plants, can flexibly adjust plant growth environment so as to easily create artistic shapes, thus completing growth technical conditions; no professional technical personnel is needed; a normal person can operate the device, and thus the plastic rack promotes life quality in places like homes and offices, and regulates people moods in certain level.