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54results about How to "Growth is not affected" patented technology

Soil conditioner for reducing cadmium and lead pollution to crop

The invention relates to a soil conditioner for reducing the cadmium and lead pollution to crops, comprising inorganic components and organic components, wherein the effective components of the inorganic components are Ca(OH)2 and Na2SiO7 or mixtures of the Ca(OH)2 and the Na2SiO7; and the organic components include rice straws and tephrosia caudida casts or mixtures of the rice straws and the tephrosia caudida casts. The soil conditioner is used before the crops are cultivated; the inorganic components are prepared into agricultural-grade powder and 0.5-4 g of the inorganic components is used for one kilogram of wind drying soil; and the grain diameter of the crushed organic components is less than 1 cm and 5-40 g of the organic components is used for one kilogram of wind drying soil. The soil conditioner effectively reduces the biological effectiveness of heavy metal, the absorption of cadmium and lead by the crops and the heavy metal pollution to the crops and especially leafy vegetables without reducing the production property of the corps.
Owner:SUN YAT SEN UNIV

Culture medium and propagation method for tissue culture rapid propagation of leaves of heuchera micrantha

The invention discloses a culture medium and a propagation method for tissue culture rapid propagation of leaves of heuchera micrantha. Through selection on an explants, selection on a basic culture medium and hormone combination in proper dosage, high-effective regeneration of a plant of the heuchera micrantha during the tissue culture is achieved. The propagation method is simple operation and low pollution rate, is high in success rate of regeneration of the plant, and is short in production period of grown-up seedlings.
Owner:ZHEJIANG XIAOSHAN COTTON & FLAX RES INST

Method for integrating exogenous gene into sheep listeria genome

The invention relates to a method for integrating an exogenous gene into a sheep listeria genome. The method comprises the following steps of: electro-transforming a first target recombinant plasmid into a first recombined bacterium, and then performing single / double homologous recombination hybridizing cultivation on the bacterium which is obtained after transforming, thereby obtaining a first target bacterium, wherein the first target bacterium is a recombined sheep listeria, the exogenous gene is integrated with an orfBAldh gene in the genome, and a code protein of the exogenous gene is stably expressed and secreted; or electro-transforming a second target recombinant plasmid into a second recombined bacterium, and then performing single / double homologous recombination hybridizing cultivation on the bacterium which is obtained after transforming, thereby obtaining a second target bacterium, wherein the second target bacterium is a recombined sheep listeria, actA gene and plcB gene are not in the genome, the exogenous gene is integrated with the orfBAldh gene in the genome, and the code protein of the exogenous gene is stably expressed and secreted.
Owner:南京颂悦生物科技有限公司 +1

Method for integrating exogenous gene into sheep listeria genome

The invention relates to a method for integrating an exogenous gene into a sheep listeria genome. The method comprises the following steps of: electro-transforming a first target recombinant plasmid into a first recombined bacterium, and then performing single / double homologous recombination hybridizing cultivation on the bacterium which is obtained after transforming, thereby obtaining a first target bacterium, wherein the first target bacterium is a recombined sheep listeria, the exogenous gene is integrated with an orfBAldh gene in the genome, and a code protein of the exogenous gene is stably expressed and secreted; or electro-transforming a second target recombinant plasmid into a second recombined bacterium, and then performing single / double homologous recombination hybridizing cultivation on the bacterium which is obtained after transforming, thereby obtaining a second target bacterium, wherein the second target bacterium is a recombined sheep listeria, actA gene and plcB gene are not in the genome, the exogenous gene is integrated with the orfBAldh gene in the genome, and the code protein of the exogenous gene is stably expressed and secreted.
Owner:南京颂悦生物科技有限公司 +1

Pellet coating method for Trifolium repens L. seed

InactiveCN101473722AQuality assuranceThe level of coating technology has improvedBiocideAnimal repellantsLiquid jetFiber
The invention relates to a pelleting coating method for Trifolium repens seeds and creates conditions for mechanization of grassland construction by carrying out pill coating on the Trifolium repens seeds. The method comprises the following steps: (1) processing of the Trifolium repens seeds: prior to the pill coating, the Trifolium repens seeds are processed by air separation and screening; (2) preparation of a coating agent: the coating agent comprises an A type coating agent, a B type coating agent, a C type coating agent, adhesive and nutrient fluid; (3) process of pill coating of the Trifolium repens seeds: the Trifolium repens seeds processed by the procedures are put in a coating machine, the external surfaces of the seeds are covered with the adhesive evenly, and the A type coating agent is added; then, the B type coating agent is added, and simultaneously liquid jetted by a flow feeder is changed into the nutrient fluid; liquid jetting is carried out continuously, and coating powder is added for pelleting; after the B type coating agent is added, the adhesive and the C type coating agent are added; and after being taken out, the seeds are insolated or dried in time, and after the insolation or drying, the coated seeds are packaged by fiber drums.
Owner:NANJING LIUHE AGRI SCI RES INST

Carrier for improving regeneration efficiency of strawberry leaves and application of carrier

The invention belongs to the technical field of plant genetic engineering, and specifically designs a carrier for improving the regeneration efficiency of strawberry leaves and application of the carrier. An RNA interference fragments of a forest strawberry cytokinin receptor gene FveHK2 is screened from a forest strawberry, a plant inhibition expression vector GN2300-FveHK2 of the forest strawberry cytokinin receptor gene FveHK2 is constructed, then transfection is performed on transgenic engineering bacteria, the plant inhibition expression vector GN2300-FveHK2 is transferred into a diploidforest strawberry 'Hawaii 4' to obtain an RNAi expression interference strain line of the FveHK2 gene, under the condition of in-vitro leaf tissue culture, the germination time of the FveHK2 gene inhibition expression line is earlier than that of a wild type, and the budding rate of the FveHK2 gene inhibition expression line is significantly higher than that of the wild type, which indicates thatthe regeneration ability of the leaves of the forest strawberry transgenic line with the genetic transformation FveHK2 interference vector is significantly improved. The carrier for improving the regeneration efficiency of the strawberry leaves has important significance for the regeneration of strawberry leaves and the improvement of the transformation efficiency.
Owner:NANJING AGRICULTURAL UNIVERSITY

Crab-rice symbiotic planting-breeding pool and comprehensive temperature-adjusting method thereof

The invention discloses a crab-rice symbiotic planting-breeding pool and a comprehensive temperature-adjusting method thereof. By using the crab-rice symbiotic planting-breeding pool, symbiosis and coculture of river crabs and high-straw rice can be realized; the temperature of water in the crab-rice symbiotic planting-breeding pool in hot summer can be controlled below 28 DEG C, so normal feeding and molting of the river crabs in summer can be guaranteed; ventilation, air permeation and enough illumination can be obtained among the high-straw rice; pests parasitized on rice straws can be feeds for aquatic animals like the river crabs and wild fishes; the use of a pesticide is not needed; manures excreted by the aquatic animals like the river crabs and the wild fishes are used as an organic fertilizer of the high-straw rice, so benign ecological balance is realized in the crab-rice symbiotic planting-breeding pool; farmers can simultaneously obtain high-quality organic rice with high yield and large-sized river crabs with high yield; the comprehensive productivity of a crab pool is improved; the product quality is enhanced; and the economic benefits are increased.
Owner:常州市金坛区水产技术指导站

Method for rapid tissue propagation of acer saccharum

The invention discloses a method for rapid tissue propagation of acer saccharum, and belongs to the technical field of woody plant tissue culture and propagation. The method comprises the following steps: selecting explants, pretreating the explants, establishing a sterile system, performing inductive culture, performing multiplication culture, performing rooting culture, strengthening and domesticating seedlings, and the like. Tissue organs of plants are cultured under a sterile system condition by controlling external environment conditions artificially, the purpose of rapidly propagating nursery stock is achieved, and stable properties of excellent female parents are effectively maintained. Compared with common vegetative propagation of cuttage and grafting, the method is small in material amount, free of limit of natural conditions and relatively applicable to neophytes, the propagation coefficient is as high as 5-20 times, and propagation and popularization problems caused by shortage of resources of the acer saccharum are solved.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Pig feed and preparation method thereof

The invention provides a pig feed and a preparation method thereof. The pig feed comprises 1 percent by weight of premix, a synthetic amino acid and a compound enzyme, wherein the synthetic amino acid is a mixture of L-lysine, DL-methionine, L-threonine and L-tryptophan; the compound enzyme is a mixture of protease, amylase, phytase, xylanase and beta-mannase. Compared with a common pig feed, the daily pig feed prepared from the synthetic amino acid and the compound enzyme has the advantages that the daily grain and the conversion efficiency of the feed are obviously increased, and fecal nitrogen and urine nitrogen are obviously reduced; the growth speed of pigs is increased, and the environmental improvement is facilitated; the growth of animals is not affected by the added synthetic amino acid; the reduction of the crude proteins of the daily pig feed facilitates the development of intestinal tracts, the villus height of duodenum in the intestinal tracts is increased, and the digestion and the absorption of proteins are facilitated.
Owner:NANCHANG AONONG BIOLOGICAL SCI & TECH

Method for planting rice by fresh water irrigation and drainage and salt control in heavy saline-alkali land

InactiveCN109892188AGrowth is not affectedOvercoming the problem of not growing normallyClimate change adaptationRice cultivationFresh water organismRipening
The invention discloses a method for planting rice by fresh water irrigation and drainage and salt control in heavy saline-alkali land. The method comprises the following steps: 1, leveling cultivatedland with high precision; 2, preparing a water feeding and drainage system; 3, deeply loosening soil, wherein deep loosening is carried out by using a soil loosening plough, the depth is more than 60cm; 4, executing drip washing with water, wherein drip washing with water is that firstly ploughing is executed, then irrigation and soaking for 3 days is executed, and then quick water drainage is executed, and one or more times of drip washing with water is adopted according to the drip washing effect; 5, planting rice; 6, executing normal irrigation and drainage of fresh water, wherein fresh water is normally irrigated and drained at the initial stage of rice planting so as to achieve the aim of salt washing and controlling; 7, lightly baking fields and adopting no field baking; 8, improving the water quality requirement during the heading and flowering periods of the rice; 9, delaying water cut-off time, wherein water in the fields can be drained thoroughly after grain filling of therice and a yellow ripening stage comes. By adopting the method, rice can be planted in the heavy saline-alkali land, and it is proved through the practice that the growth of the rice is not affected.
Owner:江苏润顺生态农业科技有限公司

Planting method for wormwood

The invention relates to the technical field of plant planting and cultivating, in particular to a planting method for wormwood. The planting method comprises the following steps of film mulching and soil preparation, ploughing and seedling planting, field management, seedling harvest and plant dividing; thin film is placed at the bottoms of field ridges during film mulching and soil preparation, during plant dividing and seed overturning, the thin film is shaken to loosen soil on the upper portions of the field ridges, and then wormwood roots are selected out directly. According to the planting method of the wormwood, the thin film can reduce the loss of water on the upper portions of the field ridges, pests and diseases of the wormwood roots are reduced, and the damage to the wormwood roots in plant dividing and seed overturning can be prevented.
Owner:贵州务川八爷农场有限公司

Solid-liquid dual-purpose fertilizer applicator

The invention relates to the technical field of planting and fertilizer application machinery, and discloses a solid-liquid dual-purpose fertilizer applicator. The solid-liquid dual-purpose fertilizerapplicator comprises a frame, wherein the upper surface of the right end of the frame is fixedly connected with a solid fertilizer feeding tank; the interior of the lower end of the solid fertilizerfeeding tank is fixedly connected with a support frame; the end, which is away from the solid fertilizer feeding tank, of the support frame is fixedly connected with a solid stirrer; the lower surfaceof the right end of the frame is fixedly connected with a solid fertilizer discharging tank; the interior of the upper end of the solid fertilizer discharging tank is fixedly connected with a baffle;the lower portion of the baffle is movably connected with an eccentric wheel; and the upper surface of the middle of the frame is fixedly connected with a lifting mechanism. A control chamber is usedfor adjusting the rotation speed of a servo motor to control the rotation speed of the eccentric wheel so as to achieve the effect of controlling the fertilizer application amount of solid fertilizers; and by shaking a handle, the lifting mechanism rotates, and a spray head which is engaged with a thread column ascends or descends by rotation of the thread column, so that the effect of ascendingand descending the spray head is achieved.
Owner:姜卫

Straw deep burying and returning method and device

Provided are a straw deep burying and returning method and device. The method includes the following steps of firstly, conducting picking and pulverizing through a deep burying and returning device; secondly, breaking original ridges into furrows through a ditching plow in the device after picking and pulverizing are completed, meanwhile making a deep burying plow in the device go deep into furrowbottom layers, and scattering pulverized straw discharged from a feeding box into a plow cavity of the deep burying plow onto the furrow bottoms through a deep burying conveyer so that the deep burying of the straw can be completed and the formed furrows can serve as the furrows for the tillage in the next season; thirdly, making loose soil on the upper side and the two sides intermediately falland converge to evenly bury the straw scattered on the furrow bottoms when the deep burying plow passes through the soil in the furrow bottoms, and combining original furrows into ridges as the foundation parts for the plantation in the next season. By means of the method and device, the alternate straw pulverizing and deep burying operation is conducted, the problems in an existing straw pulverizing and burying technology are successfully solved, and the straw returning method is efficient.
Owner:朱建国

Intelligent maintenance planting box

The invention discloses an intelligent maintenance planting box. The intelligent maintenance planting box comprises a box body, an absorbent cotton rope, a fertilizing assembly, a sun shield, a plant growth lamp, a rainwater sensor, a drying assembly, a constant-temperature adjusting device and a soil detector, and a planting chamber storing soil, a water storage chamber storing water and a fertilizer storage chamber storing liquid fertilizer are sequentially formed from top to bottom; one end of the absorbent cotton rope is arranged in the water storage chamber, and the other end is arranged in soil; the fertilizing assembly is connected with the fertilizer storage chamber and the planting chamber through a pipeline; the sun shield covers the top of the planting chamber, the first end of the sun shield is hinged to the box body, the second end of the sun shield is connected to a telescopic assembly, the other end of the telescopic assembly is connected to the box body, and the sun shield is opened or closed through the telescopic assembly; the plant growth lamp is arranged in the planting chamber; the rainwater sensor is used for sensing rainwater and is arranged on the sun shield; the drying assembly is arranged in the planting chamber and used for blowing out hot air to dry air and soil; the constant-temperature adjusting device is arranged in the soil and used for heating or refrigerating the soil to keep the soil at a constant temperature; and the soil detector is arranged in the soil and used for detecting the temperature, humidity and fertility of the soil. The intelligent maintenance planting box can realize automatic maintenance of plants, and is very convenient.
Owner:SHANGHAI INST OF TECH

Device applicable to mycorrhizal seedling cultivation under open-air condition and method

The invention provides a device applicable to mycorrhizal seedling cultivation under an open-air condition, belonging to the technical field of cultivation of plant seedlings. The device is composed of a container body, a light-shielding, rain-blocking and dust-preventing cover and a water guiding unit. According to the invention, an aseptic seedling is firstly cultivated by utilizing the container body designed by the invention; a microbial inoculum is introduced to the root system of the aseptic seedling from the positions of inoculation holes of the container body; through arrangement of the water guiding unit and wrapping with the light-shielding, rain-blocking and dust-preventing cover, the container body is moved to the outside; thus, cultivation of a mycorrhizal seedling under the open-air condition is realized. The device provided by the invention can realize anti-pollution cultivation of the mycorrhizal seedling without application of greenhouse facilities or indoor conditions, i.e., under the open-air condition, solves a series of technical problem of large-scale, low-cost and high-quality cultivation of the mycorrhizal seedling, can also be used for nursery seedling raising in an arid region, can greatly save consumption of water, and improves the survival rate of a seedling.
Owner:LISHUI ACAD OF FORESTRY

Pellet coating method for Trifolium repens L. seed

InactiveCN101473722BLess consumption per unit areaReduce import billsBiocideAnimal repellantsFiberLiquid jet
The invention relates to a pelleting coating method for Trifolium repens seeds and creates conditions for mechanization of grassland construction by carrying out pill coating on the Trifolium repens seeds. The method comprises the following steps: (1) processing of the Trifolium repens seeds: prior to the pill coating, the Trifolium repens seeds are processed by air separation and screening; (2) preparation of a coating agent: the coating agent comprises an A type coating agent, a B type coating agent, a C type coating agent, adhesive and nutrient fluid; (3) process of pill coating of the Trifolium repens seeds: the Trifolium repens seeds processed by the procedures are put in a coating machine, the external surfaces of the seeds are covered with the adhesive evenly, and the A type coating agent is added; then, the B type coating agent is added, and simultaneously liquid jetted by a flow feeder is changed into the nutrient fluid; liquid jetting is carried out continuously, and coatingpowder is added for pelleting; after the B type coating agent is added, the adhesive and the C type coating agent are added; and after being taken out, the seeds are insolated or dried in time, and after the insolation or drying, the coated seeds are packaged by fiber drums.
Owner:NANJING LIUHE AGRI SCI RES INST

Method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi regulation

The invention provides a method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi (clustered regularly interspaced short palindromic repeats i) regulation. According to the method, an acetaldehyde dehydrogenase coding gene of the corynebacterium glutamicum is replaced through a dCas9 expression cassette gene; a lactic dehydrogenase encoding gene of corynebacterium glutamicum is replaced by sgRNA transcription units of an isocitrate dehydrogenase encoding gene and a malic acid synthetase encoding gene, an isocitrate lyase encoding gene of the corynebacterium glutamicum is over-expressed to obtain a corynebacterium glutamicum engineering strain, and the engineering strain is cultured to ferment and synthesize glyoxylic acid. Corynebacterium glutamicum is used as a starting strain, a lactic dehydrogenase coding gene and an acetaldehyde dehydrogenase coding gene which are key enzymes of branch metabolism of the corynebacterium glutamicum are knocked out, a CRISPRi regulation system is established, expression levels of an isocitrate dehydrogenase coding gene and a malic acid synthase coding gene are reduced, and meanwhile, an isocitrate lyase coding gene is overexpressed, so that a method for biosynthesizing glyoxylic acid by using corynebacterium glutamicum is established.
Owner:HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY

Multi-stage purification ecological wave-eliminating system for high stormy wave and low transparency water body area

ActiveCN108585211ASave powerReduce resuspensionBarrages/weirsDamsShoreBenthos
The invention relates to a multi-stage purification ecological wave-eliminating system for a high stormy wave and low transparency water body area, and particularly relates to the technical field of water environment ecological repairing. The system comprises an ecological submerged dike, the ecological submerged dike comprises a submerged dike body and benthonic organisms, wherein the benthonic organisms are put on the submerged dike body; the submerged dike comprises a first submerged dike and a plurality of second submerged dikes, and the first submerged dike is arranged at a preset distance from the shore side in the length direction along the shore side; the secondary dikes are connected with the first submerged dike at intervals and extended in the lake center direction; submerged plants are included, and the submerged plants are planted at the position between the shore side and the first submerged dike; floating leaved plants are further included, and the floating leaved plantsare planted at the lake gaps between the first submerged dike and the second submerged dikes. According to the system, the system has a wave-eliminating effect, and water bodies can be purified.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI

A method for efficiently synthesizing PHB by knocking out flagella and pilus genes

The invention discloses a method for efficiently synthesizing PHB by knocking out flagellum and pilus genes, which belongs to the field of gene engineering and fermentation engineering. According to the invention, three flagellum gene clusters flhE-motA, fliY-fliR and flgN-flgL on an escherichia coli genome are knocked out to obtain a flagellum simplified strain WJW010, and a fimbriae gene clusterfimB-fimH is knocked out to obtain a fimbriae simplified strain WJW011, the three genes synthesized by PHB can be transformed into strains WJW010 and WJW011, and the recombinant bacteria WJW010 / pBHR68 and the recombinant bacteria WJW011 / pBHR68 are obtained; pHB with the cell dry weight of 19.2% and 2.8% can be synthesized under the normal fermentation condition, and the volume yield of PHB is 9.6times and 1.4 times that of wild control bacteria W3110 / pBHR68 (2%).
Owner:JIANGNAN UNIV

FLP/FRT gene knockout method of Candida amazonensis

The invention discloses an FLP / FRT gene knockout method of Candida amazonensis and belongs to the technical field of gene engineering. The FLP / FRT gene knockout method comprises the following steps: putting an FLP recombinase gene under a strictly induced type promoter SpGAL1p or SpMAL1p and regulating and controlling; adding directly repeated FPT sites to two ends of an FLP expression box and a resistance marker expression box; constructing a gene knockout box by taking PDC as a target gene and integrating the gene knockout box into a Candida amazonensis genome; then, adding an induction agent to induce FLP expression, so as to realize excision of resistance genes. According to the FLP / FRT gene knockout method disclosed by the invention, marker-free gene knockout of the Candida amazonensis is realized for the first time, and the method can be used for continuously knocking out a plurality of genes; a powerful technical means is provided for modification of metabolism engineering of the Candida amazonensis.
Owner:JIANGNAN UNIV

Seedling raising device for preventing other bacterium infection and application thereof

The invention relates to a seedling raising device for preventing other bacterium infection. The device comprises a seedling raising container composed of a bottom surface and a side surface of a container. The device is characterized in that air holes and inoculation holes are formed in the side wall of the seedling raising container, a supporting ring is fixedly connected to the bottom surface of the seedling raising container, and a medium baseplate is cooperatively arranged on the supporting ring; drain holes are formed in the bottom surface of the seedling raising container and the side wall of the seedling raising container of the medium baseplate. The device can also be cooperatively provided with a light and rain shielding dustproof cover and a water guide device. The designed seedling raising device is simple in structure and convenient to use, mycorrhiza seedlings can be cultured under an open-air condition instead of a greenhouse facility or an indoor condition, the serial technical aim of large-scale, low-cost and high-quality culture of the mycorrhiza seedlings is achieved, the seedling raising device can also be used for nursery seedling raising at a drought region, the water can be greatly saved, and the survival rate of nursery stocks is increased.
Owner:LISHUI AGRI SCI

Sunflower interplanting spring wheat plantation method before sowing

The invention belongs to the agriculture plantation technical field, and especially relates to a sunflower interplanting spring wheat plantation method before sowing; a conventional planting mode has two crop symbiosis stage short problems; the method wholly uses a base fertilizer, separates plots, and plants crops in sections, thus improving the sunflower and wheat production yield and quality; the method can fully utilize sunlight, heat and soil resources, can realize one-season-two-cropping, can reduce land surface exposure, can match quality seeds with fine method, can combine farm machinery with agronomic process, thus fully increasing output, quality and benefits, increasing yield and incomes, and protecting farmland ecology environment; the method is strong in practicality and wide in application prospects.
Owner:甘肃省农业科学院作物研究所

Nutritional additive capable of reducing harm of heavy metals and used in nursery stage and preparation method of nutritional additive

The invention discloses a nutritional additive capable of reducing harm of heavy metals and used in a nursery stage. The nutritional additive capable of reducing harm of the heavy metals and used in the nursery stage is prepared from the following raw materials in parts by weight: 23-25 parts of peat soil, 18-22 parts of pollen pini, 3-5 parts of silicate sulfate, 2-3 parts of cysteine, 24-26 parts of cavings, 21-23 parts of fish meal, 7-9 parts of ammonium bicarbonate, 39-41 parts of volcanic, 63-68 parts of waste crop straws, 5-7 parts of composite fungal hyphae, 18-22 parts of threonine mother liquor, 16-18 parts of old tea leaves, and a proper amount of water. High adsorption performance of fermentation products such as the peat soil and the cavings is adopted, by substances such as polyphosphoric acid and organic acid which are secreted by fungi, a large amount of residual heavy metals in soil can be adsorbed in a complexing manner, toxicity of the heavy metals is weakened, the number of heavy metals which are transported to a ground part to a seedling root is reduced, therefore, seedlings of crops are protected against being poisoned by the heavy metals, healthful growth of the crops is guaranteed, and safety of later crop products is improved.
Owner:FENGYANG JIAHE AGRI TECH

Application of metformin targeting COX6B2 in the preparation of drugs for the treatment of pancreatic cancer

The invention discloses an application of metformin targeting COX6B2 in the preparation of a drug for treating pancreatic cancer (PDAC). Metformin reduces the level of mitochondrial oxidative phosphorylation through mitochondrial COX6B2, resulting in weakened energy metabolism of PDAC, thereby inhibiting the invasion and migration of PDCA cells. The present invention constructs different PDAC cell models, uses metformin to treat the PDAC cell lines, and discovers the mechanism of action between metformin, COX6B2, oxidative phosphorylation and PDAC. This discovery can not only provide effective molecular markers for the diagnosis of PDAC, It will also provide theoretical guidance for drug target design and drug selection for PDAC treatment, which has important clinical significance and social value.
Owner:WENZHOU MEDICAL UNIV

A kind of FLP/FRT gene knockout method of candida amazonensis

The invention discloses an FLP / FRT gene knockout method of Candida amazonensis and belongs to the technical field of gene engineering. The FLP / FRT gene knockout method comprises the following steps: putting an FLP recombinase gene under a strictly induced type promoter SpGAL1p or SpMAL1p and regulating and controlling; adding directly repeated FPT sites to two ends of an FLP expression box and a resistance marker expression box; constructing a gene knockout box by taking PDC as a target gene and integrating the gene knockout box into a Candida amazonensis genome; then, adding an induction agent to induce FLP expression, so as to realize excision of resistance genes. According to the FLP / FRT gene knockout method disclosed by the invention, marker-free gene knockout of the Candida amazonensis is realized for the first time, and the method can be used for continuously knocking out a plurality of genes; a powerful technical means is provided for modification of metabolism engineering of the Candida amazonensis.
Owner:JIANGNAN UNIV

Method for cultivating Lactarius akahatsu mycorrhizal seedling

The invention provides a method for cultivating a Lactarius akahatsu mycorrhizal seedling. The method is characterized by comprising the following steps: 1) preparation of a seedling cultivation device: 2) cultivation of an aseptic seedling; 3) preparation of a microbial inoculum; 4) inoculation; 5) addition of a seedling cultivation substrate; 6) arrangement of a water guiding device and a light-shielding, rain-blocking and dust-preventing cover; and 7) cultivation of a mycorrhizal seedling. By adoption of the device designed by the invention for cultivation of the Lactarius akahatsu mycorrhizal seedling, the infection rate of miscellaneous bacteria is low, and the infection rate of puffball is zero.
Owner:LISHUI ACAD OF FORESTRY

Externally-hung marker plate for artificial breeding of flatfish fry and marking method

The invention discloses an externally-hung marker plate for artificial breeding of flatfish fry, which belongs to the technical field of culture and proliferation of marine biological resources. The externally-hung marker plate for the artificial breeding of the flatfish fry comprises an external marker end, a connecting line and an anchoring end, wherein the external marker end consists of a marker rod and an outer sleeve; encoding information is printed on the marker rod; the outer sleeve is tightly sleeved outside the marker rod; the marker end and the anchoring end are connected together by the connecting line; the connecting line is connected with one end of the marker end to form a straight line; and the other end of the connecting line is vertically connected with the anchoring end. The externally-hung marker plate for the artificial breeding of the flatfish fry is simple in structure, convenient to manufacture, light in weight and easy to operate; and the marker rod is coated by the transparent polypropylene outer sleeve and the outer sleeve is good in corrosion resistance, so that the information difficultly shed due to corrosion. The invention further provides a marking method used for the externally-hung marker plate for the artificial breeding of the flatfish fry. The externally-hung marker plate for the artificial breeding of the flatfish fry is anchored on the backs of the flatfish fry by using a special marking machine; the marked flatfish fry is high in survival rate; the plate-shedding rate is low; the externally-hung marker plate for the artificial breeding of the flatfish fry has small influence on the growth and the food intake amount of the marked flatfish fry; and the marking method is simple in operation and is applicable to marking of flatfish bodies on a large scale.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Glass bottle plant decoration plastic rack

A glass bottle plant decoration plastic rack can enhance root survival conditions under a conventional net condition, so the plant can be formed and viewed without affecting basic plant growth; the glass bottle plant decoration plastic rack is suitable for various plants, can flexibly adjust plant growth environment so as to easily create artistic shapes, thus completing growth technical conditions; no professional technical personnel is needed; a normal person can operate the device, and thus the plastic rack promotes life quality in places like homes and offices, and regulates people moods in certain level.
Owner:XIAN SHENKE ELECTRONICS RES INST
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