Device applicable to mycorrhizal seedling cultivation under open-air condition and method
A technology for mycorrhizal seedlings and conditions, applied in the field of plant seedling raising, can solve the problems of reducing the infection rate of mycorrhizal plants, the decay of seedlings, insufficient illumination, etc., and achieve the effects of increasing the infection rate of mycorrhizal plants, reducing the loss of water, and increasing photosynthesis.
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Embodiment 1
[0031] A device suitable for cultivating mycorrhizal seedlings under open-air conditions designed by the present invention is mainly composed of the following three parts: a container body 1 for cultivating sterile seedlings and mycorrhizal seedlings, and a shading, rainproof and dustproof for covering the container body Cover 2, and a water guiding device 3 for injecting water to the mycorrhizal shoots.
[0032] The designed container body 1 of the present invention sees Figure 4 Air vents 1-1 are set on the side wall of the container body 1 to achieve better air permeability of the root system of the seedling substrate plant and meet the respiration needs of mycorrhizal fungi and roots; the side wall of the container body 1 is also provided with an inoculation hole 1-2 It is convenient to punch holes and inoculate with the target bacteria. In order to avoid direct contact between the bottom of the seedling container and the nursery ground, and to prevent the native mycorrh...
Embodiment 2
[0043] Adopt the device in the embodiment 1 to inoculate and cultivate the slash pine symbiosis seedling of Boletus spp., the method is as follows:
[0044] 1. Bacteria preparation:
[0045] 1) Strain tissue isolation: Collect the fruiting body of Boletus cerevisiae in Chengxi Village, Liandu District, Lishui City, and bring it back to the laboratory for tissue isolation. Take the mung bean-sized tissue at the junction of the cap and the stipe, and place a special solid After culturing on the culture medium for one week, the mycelium was picked for purification and cultivation for later use. The formula of the special solid medium is: 100g of potatoes is boiled and filtered to extract juice, 25g of glucose, 1.5g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 1g of ammonium tartrate, 0.5ml of 1% ferric citrate, 2.5ml of 0.1% zinc sulfate , vitamin B1 10mg, agar 15g, add water to 1000ml.
[0046] 2) Propagation of strains: Take the cultured strains and cut 1cm wit...
Embodiment 3
[0058] Adopt the device in the embodiment 1 to inoculate and cultivate the Pinus massoniana symbiosis seedling of orange milk mushroom, method is as follows:
[0059] 1. Bacteria preparation:
[0060] 1) Separation of strains and tissues: Orange milk mushrooms were collected in the Dayangshan forest area of Jinyun County, Lishui City (Lactarius akahatsu ) fruiting body, brought back to the laboratory for tissue separation, and the mung bean-sized tissue at the junction of the cap and the stipe was taken, placed on a special solid medium for cultivation for one week, and then the hyphae were picked for purification and cultivation for later use. The formula of the special solid medium is: 200g of potatoes boiled and filtered to extract juice, 20g of glucose, 5g of brown sugar, 1.5g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 1g of ammonium chloride, 1g of peptone, 0.5ml of 1% ferric citrate , 0.1% zinc sulfate 1ml, vitamin B1 10mg, agar 20g, add water to 100...
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