Method for cultivating Lactarius akahatsu mycorrhizal seedling

Abstract

The invention provides a method for cultivating a Lactarius akahatsu mycorrhizal seedling. The method is characterized by comprising the following steps: 1) preparation of a seedling cultivation device: 2) cultivation of an aseptic seedling; 3) preparation of a microbial inoculum; 4) inoculation; 5) addition of a seedling cultivation substrate; 6) arrangement of a water guiding device and a light-shielding, rain-blocking and dust-preventing cover; and 7) cultivation of a mycorrhizal seedling. By adoption of the device designed by the invention for cultivation of the Lactarius akahatsu mycorrhizal seedling, the infection rate of miscellaneous bacteria is low, and the infection rate of puffball is zero.

Description

technical field [0001] The invention belongs to the technical field of plant seedling cultivation, in particular to a method for cultivating orange milk mushroom mycorrhizal seedlings. Background technique [0002] Mycorrhizal edible fungi such as boletus, truffle, and pine milk mushroom are important types of edible fungi. They grow symbiotically on the roots of trees to form ectomycorrhizae. They absorb photosynthetic products of plants and remove them from the soil. The absorbed nutrients are used by trees, which is a symbiotic relationship. At present, the usual way of artificially cultivating mycorrhizal edible fungi is to first cultivate symbiotic saplings in a sterilized culture medium, and inoculate the root system of aseptic seedlings with propagules such as mycelia or spores, and then inoculate the mycorrhizae after inoculation, After being cultivated in the laboratory or in the greenhouse for a period of time, the seedlings are hardened in the open air (some do n...

AI Technical Summary

Problems solved by technology

However, the main problems in the existing technology are: (1) Mycorrhizal seedling cultivation can only be cultivated in greenhouses or laboratories, it is difficult to scale production, the production operability is not strong, the investment in facilities is large, and the production cost of mycorrhizal seedlings is high

Both root dipping and sowing inoculation methods require transplanting aseptic seedlings, which may easily cause damage to the root system of the seedlings during the transplanting slow seedling stage, and the seedlings will easily die when the weather is unfavorable, resulting in inoculation failure. Although the inoculation method has little effect on the seedlings, the chance of direct contact between the inoculant and the roots of the seedlings is small, and the inoculated bacteria grow very slowly and are not easy to survive for a long time, thereby reducing the mycorrhizal infection rate

(4) After the aseptic seedlings are inoculated with bacterial agents, especially in the three months immediately after inoculation, the water management of the culture medium is very difficult and difficult to accurately grasp, which often leads to a decrease in the mycorrhizal infection rate and even the decay and death of the seedlings

(5) The currently used container for cultivating mycorrhizal seedlings is a general seedling container, which needs to be placed in a greenhouse or used under indoor conditions. It needs a fluorescent lamp to provide a light source, which consumes a lot of energy and costs, and is not conducive to large-scale cultivation of mycorrhizal seedlings.

Insufficient light under greenhouse conditions affects the photosynthesis and mycorrhizal synthesis of seedlings

(6) When the seedlings inoculated with mycorrhizal fungi were directly placed in open-air conditions using ordinary seedling-raising containers, the seedling-raising substrates were prone to dehydration, and propagules such as non-purpose mycorrhizal fungal spores in nature (can be infected by rainwater, wind or underground soil) Infection of non-target mycorrhizal fungi (such as puffballs, sclerotidia, etc.) in the substrate can easily cause contamination, resulting in the failure of the target mycorrhizal seedling cultivation

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