Method for rapid tissue propagation of acer saccharum

Abstract

The invention discloses a method for rapid tissue propagation of acer saccharum, and belongs to the technical field of woody plant tissue culture and propagation. The method comprises the following steps: selecting explants, pretreating the explants, establishing a sterile system, performing inductive culture, performing multiplication culture, performing rooting culture, strengthening and domesticating seedlings, and the like. Tissue organs of plants are cultured under a sterile system condition by controlling external environment conditions artificially, the purpose of rapidly propagating nursery stock is achieved, and stable properties of excellent female parents are effectively maintained. Compared with common vegetative propagation of cuttage and grafting, the method is small in material amount, free of limit of natural conditions and relatively applicable to neophytes, the propagation coefficient is as high as 5-20 times, and propagation and popularization problems caused by shortage of resources of the acer saccharum are solved.

Description

technical field [0001] The invention relates to a method for rapid propagation of sugar maple tissue culture, belonging to the technical field of tissue culture and propagation of woody plants. Background technique [0002] The Canadian sugar maple tree is extremely beautiful in shape and unique in leaf shape. After autumn, the leaf color begins to gradually change from green to yellow, orange yellow, colorful, deep red and other colors. It is a world-renowned ornamental tree with colorful leaves. In addition, it is also one of the three major sugar-bearing woody plants. The sap contains about 3-10% maple sugar. Generally, sugar maple trees with a diameter above 15cm can start drilling to harvest sugar solution. The continuous collection time is at least 50 years. , Known as "hardcore sugar cane". Its wood is a kind of hard maple wood, which is hard and tough, dense, fine-grained, and has extremely high abrasion resistance and steam bending resistance. It is an ideal econom...

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Problems solved by technology

For the Canadian sugar maple, cuttings and grafting are still in the experiment. The survival rate of cuttings is only 30%, and the survival rate of grafting is 5%. It is greatly affected by the natural environment such as region and temperature, and it is especially difficult to adapt to the high temperature and humidity in southern my country in summer. In the natural environment, a stable method has not yet been explored; the reproduction coefficient is not high and there are not a large number of mature trees in China to provide sufficient propagation materials, which provide certain limitations for large-scale reproduction

There are few reports on the tissue culture of Canadian sugar maple at home and abroad, but the woody tissue culture technology has been relatively mature, and its advantages of convenient material collection, controllable growth environment, high reproduction coefficient and good genetic stability are more and more used in modern times. Growing seedlings

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