6 results about "Isocitrate dehydrogenase" patented technology

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The application discloses a genetically engineered bacterium for efficiently producing itaconic acid by using corn stalk hydrolysate and application thereof, and belongs to the technical field of gene recombination and metabolic engineering. The genetically engineered bacterium for efficiently producing itaconic acid by using corn stalk hydrolysate is characterized by the following aspects: taking a strain BW08 as a starting strain, heterologously expressing a homocentric acid decarboxylase gene CAD, deleting an isocitric acid dehydrogenase gene Icd, heterologously expressing a xylose acid dehydrase gene XD, a 2-keto-3-deoxy-xylose acid dehydrase gene KDXD and an alpha-ketoglutaric acid semialdehyde dehydrogenase gene KGSADH, and heterologously expressing a citric acid synthase gene gltA. In the fed-batch fermentation, the yield of itaconic acid of the genetically engineered bacterium reaches 68.6 g / L, and the conversion rate reaches 0.6 g / g (total sugar). The genetically engineered bacterium can efficiently metabolize corn stalk hydrolysate to produce itaconic acid.

Disclosed are methods of treating of a subject having cancer comprising applying an alternating electric field to a target site of the subject for a period of time, wherein the target site comprises one or more cancer cells, wherein the subject has an isocitrate dehydrogenase mutation (IDHmut). Disclosed are methods of treating a subject comprising identifying a subject with an IDHmut; and applying an alternating electric field to a target site of the subject for a period of time, wherein the target site comprises one or more cancer cells.

The invention discloses a technology for degrading phenol and sequestering carbon by microalgae, and aims to provide a method for improving key enzyme to promote euglena to degrade phenol and enhance carbon sequestration. Comprising the following steps: performing phenol concentration gradient domestication on euglena cells, and adjusting gene expression quantities of three key enzymes, namely H < + > transport ATP enzyme, pyruvate decarboxylase and isocitrate dehydrogenase in the euglena cells; carrying out enlarged culture growth on the euglena domesticated plant, adding a proper amount of phenol into a culture medium, and continuously introducing gas containing CO2; in the culture process, the carbon sequestration rate of the euglena is calculated by measuring the carbon element content in the biomass of the euglena liquid; and after the culture is finished, measuring the content of euglena polysaccharide in the algae liquid in unit volume, and measuring the content of phenol in the algae liquid to calculate the degradation rate of phenol. According to the method, the euglena cells are domesticated by adopting phenol concentration gradient, the expression quantity of key enzyme genes is increased by several times, the phenol degradation capacity of the euglena cells is enhanced, the carbon sequestration rate of the euglena cells is increased, and the content of polysaccharide in the cells can be increased at the same time.

The application discloses a high-yield L-homoserine Corynebacterium glutamicum mutant strain and application thereof, and belongs to the technical field of fermentation engineering. The application takes Corynebacterium glutamicum ATCC13032 as a starting strain, knocks out the regulatory protein McbR, amino acid intracellular transport protein MetD, homoserine kinase, phosphoenolpyruvate carboxykinase, cysteine beta cleavage enzyme, O-acetylhomoserine sulfhydrylase, weakens isocitric acid dehydrogenase and dihydrodipicolinic acid synthase, and overexpresses pyruvate carboxylase gene mutant, aspartate kinase mutant, aspartate semialdehyde dehydrogenase, homoserine dehydrogenase, branched-chain amino acid transport protein BrnF, branched-chain amino acid transport protein BrnE, aspartate aminotransferase, bifunctional aspartate kinase / homoserine dehydrogenase 1 mutant, NADP + dependent glycerol-3-phosphate dehydrogenase. The recombinant bacteria provided by the application can reach 63.5g / L of L-homoserine yield after 96h of culture in a fermenter.

Disclosed are compounds inhibiting the conversion of α-KG to D-2-HG, pharmaceutically acceptable salts, hydrates, solvates or stereoisomers thereof and pharmaceutical compositions comprising the compounds. The compound and the pharmaceutical composition can effectively treat IDH associated diseases, including cancer.