Detection primer for human IDH (isocitrate dehydrogenase) gene mutation and reagent kit

A technology for detecting primers and kits, applied in the field of biological detection reagents, can solve the problems of unsuitable high-throughput detection and large-scale clinical application, unsuitable for large-scale promotion and use, and labor-intensive, and achieves auxiliary tumor diagnosis and low cost. , Easy to operate and fast effect
CN102732633AActive Publication Date: 2012-10-17HELIXGEN GUANGZHOU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HELIXGEN GUANGZHOU
Publication Date
2012-10-17

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Abstract

The invention discloses a detection primer for human IDH (isocitrate dehydrogenase) gene mutation and a detection reagent kit, which are used for the combination of PCR (polymerase chain reaction) and high resolution melting curve analysis technique. The detection reagent kit can be used for detecting the gene mutation on IDH 1 and IDH2 gene exon 4, detection samples are human genomes DNA (deoxyribonucleic acid) extracted from tumor tissues, blood or bone marrow cells, and the detection reagent kit is used for patients with clinical brain glioma and hematopathy to assist tumor diagnosis, treatment and judging prognosis. The detection reagent kit is easy and rapid in operation and good in detection effect.
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Description

technical field

[0001] The invention relates to a biological detection reagent, more specifically, the invention relates to a detection primer and a kit for human IDH gene mutation (including IDH1 gene and IDH2 gene). Background technique

[0002] Isocitrate dehydrogenase (IDH) is a key rate-limiting enzyme in the tricarboxylic acid cycle and plays an important regulatory role in the response to oxidative damage in cells. Since the IDH gene was found to be mutated in glioma in 2008, it has rapidly become a research hotspot. IDH gene mutation can be used as a specific indicator for the diagnosis of glioma, an effective molecular marker for grading and identification of glioma, and an independent factor for judging the prognosis of glioma patients. IDH mutations also occur in a small number of leukemias and myeloproliferative disorders (MPD) and other blood diseases.

[0003] Human IDH has three subfamilies, IDH1, IDH2, and IDH3. IDH1 is located in the cytoplasm and peroxiso...

Claims

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