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Test kit of auxiliary diagnosis of non-small cell lung cancer patients

A non-small cell lung cancer and auxiliary diagnosis technology, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of failing to meet clinical needs and low diagnostic efficiency, and achieve high sensitivity and strong specificity

Active Publication Date: 2013-06-19
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the above protein markers have been used in the auxiliary diagnosis of lung cancer, their diagnostic efficiency is low and cannot meet the clinical needs

Method used

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  • Test kit of auxiliary diagnosis of non-small cell lung cancer patients
  • Test kit of auxiliary diagnosis of non-small cell lung cancer patients
  • Test kit of auxiliary diagnosis of non-small cell lung cancer patients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, establishment of the inventive method

[0033] 1. Model establishment

[0034]952 plasma samples from NSCLC patients and 479 plasma samples from healthy people (normal controls) were collected from lung cancer patients diagnosed by pathology in the Department of Thoracic Surgery, Cancer Hospital, Chinese Academy of Medical Sciences, and normal people who participated in cancer screening. This experiment was approved by the Ethics Committee, and all subjects were informed and signed an informed consent form. Plasma samples were obtained before surgery, and the patients had not received chemotherapy, radiotherapy, or interventional treatment before.

[0035] Data processing uses curve expert 1.4 to process the standard curve; GraphPad Prism 5.01 to process the Column statistical graph; SPSS 13.0 to fit the data and establish a mathematical model; SAS 9.2 to test the mathematical model; MedCalc 9.6.2.0 version to calculate the model ROC curve and cutoff valu...

Embodiment 2

[0045] Embodiment 2, verification of the inventive method

[0046] The patients used in this example were 150 clinically diagnosed patients with non-small cell lung cancer (peripheral venous blood was obtained before surgery, and the patients had not received chemotherapy, radiotherapy, intervention, etc.) and 50 healthy people (non-small cell lung cancer). cell lung cancer patients), all volunteers with informed consent.

[0047] 1. Take 5ml of peripheral venous blood from the patient to be tested on an empty stomach, let it stand at room temperature for 1h, then centrifuge at 3000r / min for 15min, take the supernatant, divide it into two 1.5mL EP tubes, and store in a -80°C refrigerator.

[0048] 2. Dissolve the sample from step 1 at room temperature for 3 hours, then centrifuge at 3000r / min for 5 minutes.

[0049] 3. Detect the concentration of the protein marker IDH1 (in U / L), the concentration of the protein marker CA125 (in U / mL), and the concentration of the protein mar...

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Abstract

The invention discloses a test kit of auxiliary diagnosis of non-small cell lung cancer patients. The test kit comprises a product used for detecting protein landmark isocitrate dehydrogenase 1(IDH1), a product used for detecting protein landmark carbonic anhydrase 125 (CA125), a product used for detecting protein landmark CYFRA21-1 and a carrier on which a functional expression p=1 / (1+(20.186*0.406<x1>*0.923<x2>*0.656<x3>)<-1>) is recorded, wherein x1 represents the concentration of IDH1, x2 represents the concentration of CA125, and x3 represents the concentration of CYFRA21-1. The test kit is adopted and the non-small cell lung cancer patients are diagnosed in an auxiliary mode according to corresponding diagnosis standards, the test kit has the advantages of being high in sensitivity and strong in specificity, reliability of the diagnosis is far higher than that of diagnosis which is carried out with each single protein landmark, and the test kit has great value and application prospects for diagnosis and treatment of non-small cell lung cancer.

Description

technical field [0001] The invention relates to a kit for auxiliary diagnosis of patients with non-small cell lung cancer. Background technique [0002] Lung cancer is a major disease that seriously threatens human life and health. In 2011, the World Health Organization (WHO) International Cancer Research Agency IARC released the results of 2008 global statistical data, showing that lung cancer is the malignant tumor with the highest incidence and mortality. Every year, there are 1.608 million new cases and 1.377 million deaths worldwide, accounting for 12.7% and 18.2% of all malignant tumors. According to statistics released by the American Cancer Society, it is estimated that in 2012, the number of new lung cancer cases in the United States will reach 226,000, and the number of deaths due to lung cancer will reach 160,000. In my country, the results of the third national retrospective sampling survey on causes of death hosted by the Ministry of Health show that lung canc...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
Inventor 赫捷谭锋维孙楠陈照丽
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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