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Cold-resistant gene engineering application method of rice OsICE2 gene

An application method and genetic engineering technology, applied in the application field of cold resistance genetic engineering of rice OsICE2 gene

Inactive Publication Date: 2013-09-11
HUAIYIN TEACHERS COLLEGE
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a cold resistance genetic engineering application method of rice OsICE2 gene, aiming to solve the genetic engineering problem of rice cold resistance

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] The cold-resistant genetic engineering application method of the rice OsICE2 gene provided by the embodiments of the present invention comprises the following steps:

[0031] (1) Gene cloning, (2) Vector construction, (3) Transgenic, (4) Analysis of the cold resistance phenotype of OsICE2 through transgenic technology; (5) Comparative analysis of cold resistance physiology and protein expression between transgenic plants and control plants .

[0032] (1) The specific method of gene cloning is:

[0033] In the leaf cDNA of rice material Nipponbare seedlings, a gene encoding a complete prote...

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Abstract

The invention discloses a cold-resistant gene engineering application method of rice OsICE2 gene. The method includes: (1) gene cloning, (2) vector construction, (3) transgenosis, (4) analysis of the cold-resistant phenotype of OsICE2 by a transgenic technology, and (5) comparative analysis of a transgenic plant and a control plant in the cold-resistant physiological aspect. By the steps, the method determines the influence of OsICE2 gene on protein expression quantity under low temperature stress, and finds that a transgenic rice strain can slow down the reduction of protein expression quantity of RuBi sCO subunit binding-protein alpha subunit, RuBi sCO activase small isoform precursor, Chlorophyll a-b binding protein and isocitrate dehydrogenase under low temperature stress, and improves the expression increase range of 70 kDa heat shock-related protein.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a cold-resistant genetic engineering application method of rice OsICE2 gene. Background technique [0002] Chilling injury is an environmental stress factor that has an important impact on crop growth and development. Rice is the main food crop in my country, and it often encounters the problem of chilling injury at the seedling stage, which has a negative impact on rice production. Therefore, it is of great theoretical significance and application value to study the molecular mechanism of cold resistance of crops, especially rice, and to cultivate resistant germplasm. [0003] According to the different degrees of low temperature, the low temperature injury of plants is divided into chilling injury and freezing injury. Chilling injury refers to the obstacle of plant physiological mechanism caused by low temperature above 0 ℃, and freezing injury refers to the obstac...

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Application Information

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IPC IPC(8): C12N15/84A01H5/00
Inventor 杨立明胡向阳刘华清田大刚罗玉明袁彩勇徐慧芳陈桢雨
Owner HUAIYIN TEACHERS COLLEGE
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