60 results about "Carbon metabolism" patented technology

The invention is directed to materials and methods associated with polymorphic variants in two enzymes involved in folate-dependent and one-carbon metabolic pathways: MTHFD1 (5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase) and methylenetetrahydrofolate dehydrogenase (NADP+dependent) 1-like (MTHFD1L). Diagnostic and therapeutic methods are provided involving the correlation of polymorphic variants in MTBFD1, MTHFD1, and other genes with relative susceptibility for various pregnancy-related and other complications.

The invention discloses a leaf fertilizer special for tobacco, which comprises monopotassium phosphate, magnesium sulfate heptahydrate, zinc sulfate, borax, ammonium molybdate, L-malic acid, glutamic acid, brassinolide and solvent water. Inorganic and organic nutrient substances are provided for the growth of tobacco to meet the basic growth requirement of tobacco; for example, magnesium, boron and zinc are medium trace nutrient elements deficient at the tobacco zone in Hubei; the glutamic acid and the malic acid can be directly involved in certain important metabolic activities during the growth of tobacco, in particular tricarboxylic acid cycle, and promote the growth of tobacco in the later stage to timely switch from nitrogen metabolism into carbon metabolism, so as to improve maturity and quality; and the plant growth modifier, brassinolide, can induce to strengthen the immunity of tobacco and improve the virus and fungal disease resisting capability of tobacco.

The invention discloses a genetic engineering bacterium for high-yield phloroglucinol as well as a construction method and an application of the genetic engineering bacterium and belongs to the technical field of genetic engineering. The genetic engineering bacterium over-expresses carbon storage and regulation factor gene CsrB shown by SEQ ID NO.1 and co-expresses a polyketide synthase gene phlD, a multiple resistance activating factor gene marA and acetyl CoA carboxylase gene ACCase. The invention also provides the construction method and the application of the genetic engineering bacterium for the high-yield phloroglucinol. With the adoption of the genetic engineering bacterium, the yield of the phloroglucinol is firstly increased by 115.6 percent by adopting a mode of globally regulating carbon metabolism after post-transcriptional level of the genetic engineering bacterium, and the genetic engineering bacterium has a high industrial application value.

The invention discloses a method which enhances glycometabolism efficiency of Ketogulonogeniumvulgarum, increases the number of bacteria, greatly shortens the incubation period of 2-KGA (2-keto-L-gulonic acid) and improves the production efficiency by adding carbon sources including two-carbon units such as ethyl alcohol, acetaldehyde and acetic acid as well as six-carbon units such as D-sorbitol, D-mannitol and D-mannose which facilitates utilization of Ketogulonogeniumvulgarum in a mixed fermentation process of a vitamin C two-step fermentation method according to research results of Ketogulonogeniumvulgarum metabolic network analysis. In a 5L fermentation tank, the period can be shortened by 14.04%, and the yield is increased by 3.40 %. According to another application, L-sorbose is taken as a carbon source by reducing Ketogulonogeniumvulgarum, so that the efficiency for converting L-sorbose into 2-KGA is also improved. According to the method, the production process is advanced, the raw material cost is low, and the method is suitable for large-scale industrial production and is improvement and promotion of vitamin C preparation technology with a biological fermentation method in about forty years of China; and besides, harmful substance emission in a production process is prevented, pollution and public hazard to the ambient environment are avoided, and environment protection requirements are met.

The invention discloses a gene modification method for increasing the yield of phloroglucinol and application of the same, and belongs to the technical field of genetic engineering. The gene modification method comprises the following steps: obtaining a mutant strain by virtue of a mode of knocking out or inserting a global regulating factor arcA gene of an original strain, converting the state of the mutant strain to a competent state, introducing recombinant plasmids containing a polyketide synthase gene phlD, a multiple resistance activating factor marA and acetyl CoA carboxylase gene ACCase, and obtaining a recombinant cell. The invention also provides a method of producing the phloroglucinol by utilizing the recombinant cell. According to the gene modification method and application disclosed by the invention, the yield of the phloroglucinol after fermentation is firstly increased by 2.06 times by adopting a mode of globally regulating carbon metabolism after post-transcriptional level, and the gene modification method and application have high industrial application value.

The invention discloses an application of improved ATMT (Agrobacterium Tumefaciens-Mediated Transformation) to construction of trichoderma atroviride T23 deltaCrel. The application is characterized in that the genomic DNA of trichoderma atroviride T23 is taken as a template, PCR (Polymerase Chain Reaction) amplification is carried out on the Crel gene sequence, and thus upstream and downstream flanking sequences of the Crel gene are obtained; then leading in respectively to knock out upstream and downstream of plasmid pC1300qh, so as to form recombinant plasmid pC1300qh-deltaCrel; converting escherichia coli competence of the recombinant plasmid pC1300qh-deltaCrel, performing verification, screening positive clone, extracting recombinant plasmid pC1300qh-deltaCrel, leading in agrobacterium tumefaciens AGL1, and carrying out improved ATMT. By using the improved ATMT technique, a trichoderma atroviride carbon metabolism inhibiting factor CRE1-knocked-out mutant strain is successfully constructed; the T23 deltaCrel bacterial strain has the functions of promoting secretion and activity of degrading enzyme of cell walls of trichoderma spp., and indirectly improving the function of trichoderma spp. to antagonize pathogenic bacteria.

The invention relates to a method for improving acid production rate of L-phenylalanine genetic engineering bacterial. The method comprises the steps of: constructing the original engineering plasmid MDphe-2 of L-phenylalanine into a new generation of engineering plasmid MDphe-3 by adopting a PCR (polymerase chain reaction)technology; carrying out deletion constructing on a whole metabolism network regulatory gene csrA of the engineering host bacterial of the engineering plasmid MDphe-3 through a Red system; then implanting the engineering plasmid MDphe-3 in the engineering host bacterial which is in deletion of the whole metabolism network regulatory gene csrA to construct a new L-phenylalanine genetic engineering bacterial; and finally verifying and reserving the L-phenylalanine gene engineering bacterial with high hereditary stability and high acid production rate. The method has the advantages that the PEP accumulation of one of precursors of aromatic amino acid is strengthened, simultaneously the carbon metabolism flux can be converted heavily to a shikimic acid way, the large flowing of the metabolism flux of the aromatic amino acid to the synthesis of the L-phenylalanine is ensured, and the ability of the engineering bacterial to ferment the L- phenylalanine is further improved.

The invention discloses a method for improving the yield of fumaric acid and belongs to the fields of genetic engineering and fermentation engineering. The method comprises the following steps: performing codon optimization on a pyruvate carboxylase gene RoPYC of rhizopus oryzae to obtain a gene RoPYC* and then mutating a R485 site of the gene RoPYC* into proline. In comparison with the yield after the expression of a wild type pyruvate carboxylase before codon optimization, the yield is improved by 24.6 percent; 10 percent of carbon dioxide is additionally introduced when genetically-engineered bacteria of an expression mutant is fermented for 36 hours in a fermentation tank of 7L, so that the yield of the fumaric acid reaches 453.6 mg/Lm and is improved by 20 percent through comparison. According to the method disclosed by the invention, a synthetic route through which a carbon metabolic flow enters the fumaric acid from pyruvic acid is effectively enhanced, so that conditions are created for the construction of engineering yeasts to efficiently produce the fumaric acid and other dicarboxylic acid, therefore, the industrial application value and the prospect are good.

The invention uses Bacillus as a starting strain, uses a carbon metabolism response element cre in a maltose promoter to transform a target, a lysine ribose switch is fused at the same time, a mutantof the maltose promoter is constructed, the mutant that can reduce the leaky expression of the maltose promoter and improve the induced expression strength of the maltose promoter is obtained, and anexpression vector and host cell which contain the mutant of the promoter are constructed, so as to be conducive to the application of the maltose promoter in protein expression.

The invention relates to the field of gene engineering and discloses a metabolic pathway reconstruction method for reducing lignin content and a use thereof. The metabolic pathway reconstruction method can construct a 2-phenylethyl alcohol synthesis pathway in the plant so that lignin synthesis is reduced and thus fiber biomass conversion efficiency is improved. The 2-phenylethyl alcohol synthesis pathway is from yeast and comprises three key genes of amino acid transaminase gene ARO9, pyruvate decarboxylase gene ARO10 and ethanol dehydrogenase gene ADH2. The 2-phenylethyl alcohol synthesis pathway is constructed in arabidopsis thaliana, and through competition between 2-phenylethyl alcohol synthesis and lignin synthesis in initial substrate phenylalanine use, carbon metabolism shunting is caused and synthesis of lignin in the transgenic plant is reduced. The method provided by the invention can reduce fiber biomass pretreatment difficulty by reducing plant lignin synthesis. At present, the method can reduce lignin synthesis in arabidopsis thaliana and can improve glucose release efficiency of stem cell wall extract enzymolysis.

The invention discloses the application of a purple acid phosphatase gene GmPAP35 of a soybean, and belongs to the field of gene engineering. Through functional analysis of the GmPAP35 in a whole conversion material of the soybean, a function of the GmPAP35 participating in carbon metabolism in a plant is specified. Overexpression of the GmPAP35 can increase the height of a soybean plant and the contents of glucose and fructose in the plant body, so that the soybean biomass and the phosphorus uptake are increased, and the soybean phosphorus efficiency is improved; a theoretical basis is laid for culturing a new variety of phosphorus efficient-transgenic crops. The purple acid phosphatase gene GmPAP35 has the capacity of promoting the carbon metabolism of the crops through a transgenic technology, so that the plant phosphorus efficiency is improved, and new purposes of fertilizer use amount reduction and high yield are achieved. The purple acid phosphatase gene GmPAP35 has an importanttheoretical and practical significance on developing environmentally-friendly sustainable agriculture.

The invention discloses a method for obtaining metabolic flux of an intracellular central carbon metabolic pathway in an unsteady state of metabolic steady-state isotopes. The method adopts a batch culture module, a constant culture module, a sampling module and a sample processing and analyzing module. When the OUR and the CER in the batch culture module decline at the same time, culture is performed in the constant culture module. The constant culture module specifically adopts a process of feeding a nutrient solution containing no isotope labeling substrate, starting waste discharge at thesame time, carrying out constant culture, until the microbial cells are in a metabolic steady state, feeding a nutrient solution containing the isotope labeling substrate, and keeping culture conditions unchanged; and when the isotope labeling substrate flows into a reactor, starting rapid and continuous sampling, and entering the sampling module at the same time. According to the method, a seriesof fermentation liquor samples are obtained in a short time, and then the samples are processed and analyzed to obtain accurate intracellular metabolite isotope abundance data; and the method plays acrucial role in calculating the metabolic flux.

The invention discloses an internal guide type bacterium-alga integrated microbial fuel cell ecological water purification system. The system includes a water inlet pipe, a double-layer pool body, a porous water-collecting pipe, aquatic plants, a rotatable angle fitting and a U-shaped water-discharging pipe; the double-layer pool body includes an electricity-generating inner layer, a no-electricity generation outer layer and a free water layer; aquatic algae are grown in the free water layer; sewage enters the bottom of the electricity-generating inner layer, and organic pollutants in the sewage are degraded by electricity-generating microorganisms; a water flow flows upwards, and nitrification and denitrification occur; the water flow enters the no-electricity generation outer layer fromwater passing holes in the upper part of the free water layer, and reaeration is accelerated; and the no-electricity generation outer layer intercepts the aquatic algae in the free water layer throughthe filtration effect of a filler, and pollutants in the water are further removed. According to the system provided by the invention, a microbial fuel cell technology and a traditional ecological water purification technology are integrated, the electricity-generating microorganisms and the purification function of the algae are coupled, and high-efficiency organic carbon metabolism and removalof nitrogen and phosphorus are compactly controlled in the same ecological water purification system.

The invention discloses an internal conductive bacteria and algae integrated microbial fuel cell ecological water body purification method. An ecological water body purification system is establishedand comprises a water inlet pipe, a double-layer pool body, a porous water collection pipe, aquatic plants, a rotatable elbow and a U-shaped drain pipe; the double-layer pool body comprises an electricity generation inner layer, a non-electricity-production outer layer and a free water layer; hydrobiontic algae grows in the free water layer; sewage enters the bottom of the electricity generation inner layer, and organic pollutants in the sewage are degraded by electricity production microorganisms. Water flow flows upwards and nitrification and denitrification nitrogen removal occurs. The water flow enters the non-electricity-production outer layer from a water through hole above the free water layer, and reoxygenation is accelerated; the non-electricity-production outer layer intercepts the hydrobiontic algae in the free water layer by filtering of a filler so as to further remove pollutants in water. According to the invention, a microbial fuel cell technology and a conventional ecological water body purification technology are integrated, purification functions of the electricity production microorganisms and the algae are coupled, and high-efficiency organic carbon metabolism and removal of nitrogen and phosphorus are compactly controlled in the same ecological water body purification system.

The invention relates to a post-emergence composite herbicide for corn and an application of the post-emergence composite herbicide, which belongs to the technical field of herbicide production. The post-emergence composite herbicide for corn consists of herbicide and zinc fertilizers according to the weight ratio of 1:1.5. The use method of the post-emergence composite herbicide for corn comprises the following steps: dissolving the post-emergence composite herbicide for corn in water which is 200 times of zinc sulfate in weight; and taking an aqueous solution to spray the stem leaves of corn seedlings in the seedling stage of corn. The post-emergence composite herbicide for corn provided by the invention has the advantages of promoting seedling to premature, strengthening the plant, enhancing the disease resistance and phytotoxicity resistance, improving the carbon metabolism, synthesis of amino acids and activity of enzymes, and promoting nitrogen absorption and transfer as the zinc fertilizers are added into conventional herbicide, a proper amount of zinc element in the use process of post-emergence composite herbicide for corn is added, and zinc is added into the post-emergence herbicide sprayed.

The invention relates to a recombinant strain for producing Cembrenediol and application of the recombinant strain, wherein the recombinant strain capable of producing Cembrenediol from plants is constructed by heterologous expression of a GGPP synthase gene gpps and a Cembrenediol synthase gene cbts. The yield of Cembrenediol is improved by overexpressing key genes of carbon metabolism flux in a DXP (Dixanthone) pathway, namely a 1-deoxy-D-xylulose-5-phosphate synthase gene dxs and an isoprene pyrophosphate isomerase gene idi. The expression plasmid is further optimized to excessively express dxs and idi, and the yield of Cembrenediol is increased to 2.60 times of that of a control group. By adopting the high-yield Cembrenediol strain and the method disclosed by the invention, the Cembrenediol can be efficiently produced, and the application of the strain in the fields of agriculture, tobaccos and the like can be promoted.

The invention relates to a recombinant strain of Bacillus subtilis, wherein pyruvate carboxylase BalpycA, glyceraldehyde-3-phosphate ferredoxin dehydrogenase gor, isocitrate NAD⁺ dehydrogenase icd, malate quinone dehydrogenase mqo, pyruvate ferredoxin oxidoreductase porAB and nitrogenase ferritin cyh are integrated and expressed in the recombinant strain. The invention also discloses use of the recombinant strain in fermentation production of acetylglucosamine. The recombinant Bacillus subtilis of the invention eliminates the central carbon metabolism overflow of the Bacillus subtilis and balances the intracellular reducing force, and the fermentation yield of acetylglucosamine is greatly improved.

The invention provides an intelligent regulation and control method for carbon metabolism flow of xylitol produced by escherichia coli, and aims to realize efficient synthesis of xylitol in a process of preparing xylitol by utilizing a cell factory. According to the invention, a strain of SecY engineered Escherichia coli is successfully constructed by introducing a SecY non-specific sugar transport channel and simultaneously overexpressing heterologous xylose reductase (CbXR). The strain can overcome the CCR effect through self-regulation and relieve the inhibition effect of the 5-phosphate xylitol on xylose transported by the strain. By taking the change of an external carbon source as a response signal, the CCR effect and the inhibition effect of the xylitol 5-phosphate are converted into two important regulating switches in a carbon metabolism network, and the metabolic capability of cells on glucose and xylose is regulated. The method can completely metabolize glucose and xylose in any proportion in a substrate, ensures the maximum conversion efficiency of energy to a target product, and better meets the requirements of green biological manufacturing.

The invention discloses an enzymatic activity improved pyruvate carboxylase mutant N1078F and an application thereof and belongs to the fields of genetic engineering and fermentative engineering. According to the mutant N1078F and the application thereof, a locus N1078 of pyruvate carboxylase of Rhizopus oryzae is mutated into phenylalanine, so that the enzymatic activity of the obtained mutant is improved by 16.5%. A gene FUM1 is knocked out on the basis of knocking out PDC1 and ADH1, and meanwhile, the pyruvate carboxylase mutant N1078F is excessive, so that the yield of fumaric acid is increased by 20.5%. According to the mutant N1078F and the application thereof, a way of synthesis of carbon metabolic flux into fumaric acid from pyruvic acid is effectively strengthened, and conditions for constructing engineering yeasts and efficiently producing fumaric acid and other dicarboxylic acids are created, so that the mutant N1078F has very good industrial application values and prospects.

Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.