84 results about "Lignin synthesis" patented technology

The invention discloses a solid-phase preparation method for nano zinc oxide through a template and relates to a solid-phase preparation method for nano zinc oxide. The method comprises the following steps: first, synthesizing lignin into lignin amine; and then, performing solid-phase preparation for nano zinc oxide taking the lignin amine as the template. According to the invention, the reticular lignin amine is prepared from the lignin through Mannick reaction, the advantages of being low in cost, nontoxic, easy to get and the like can be achieved; some base groups of the lignin amine are removed through high-temperature roasting, a pore structure is left, so that the specific surface area of the nano zinc oxide obtained from the lignin amine as a template agent is increased. Therefore, the method provided by the invention has the advantages of simple synthesis process, uniform particle size, controllable dynamics, low cost, easiness in operation, high yield, and low pollution, and reduces the hard aggregation appearance. The product prepared from the method is ice-cream, and adopts the structure of lead zinc ore of hexagonal system, and is suitable for industrialized production.

The invention provides a new CkMYB4 transcription factor and its gene. The gene originates from Leguminosae Caragana shrubs Caragana korshinskii Kom., and codes R2R3 MYB transcription factors. The full length cDNA sequence of the gene comprises 1106bps, comprises a 798bp open reading frame, and codes 266 amino acids. The cDNA sequence obtained after cloning is used for constructing a plant expression vector, and the vector is transformed into wild Arabidopis thaliana to obtain a transgenic plant. Transgenic Arabidopis thaliana lignin synthetase gene and lignin content detection results show that the gene provided by the invention can change the expression of the plant lignin synthesis gene and the lignin content.

The invention discloses a lignin-related protein as well as its gene encoding and application. The lignin-associated protein has one of the following amino acid residues sequences: (1) SEQ ID No. : 2 in the sequence table, and (2) lignin-related protein after SEQ ID No. : 2 one or several amino acid residues replaced and / or deleted and / or added in the sequence table. The lignin-related protein gene can regulate the content and composition of rice lignin without influences on plant growth and development, and improve the values as feed and industrial raw material of paper. In addition, the lignin-related protein gene also has important theoretical value in revealing the mechanism of plant lignin biosynthesis.

The invention discloses pear PbrmiR397a and application thereof. The microRNA gene (PbrmiR397a) is separated from Dangshan pear and has a function of regulating pear fruit sclereid development, and a precursor nucleotide sequence of the gene is shown as SEQ ID No.1. PbrmiR397a is converted into tobacco through an agrobacterium-mediated genetic transformation method to obtain a transgenic plant, biological function verification shoes that the cloned PbrmiR397a gene has a function of reducing lignin synthesis. A new gene resource is provided for molecular breeding with lignin synthesis reduced by the finding of the PbrmiR397a. The PbrmiR397a gene has the advantage of targeting and regulating multiple genes at the same time, so that a more efficient approach is provided for molecular breeding.

The invention discloses a modified lignin and a urea-formaldehyde resin synthesized from same and preparation methods of the modified lignin and the urea-formaldehyde resin, belonging to the field of bonding agents used in wood processing. A technological synthetic route with weak base-weak acid-weak base is adopted, the molar ratio of formaldehyde to urea is controlled during an addition stage to generate a reasonable structure, the modified lignin as well as residual hydroxymethyl and free formaldehyde are added at the later stage of reaction in order to accomplish reaction, thus the content of methylene ether link (-CH2-O-CH2-) and the content of hydroxymethyl in the resin are reduced to a certain extent, the formaldehyde emission of the resin is lowered, and simultaneously, higher bonding strength and water resistance are ensured. The urea-formaldehyde resin in the invention is simple and brief in process and low in cost, the formaldehyde emission of the plate prepared by adoptingthe bonding agent reaches the E0-level standard and the plate can maintain high bonding strength after being cooked in hot water. The modified lignin is simple in synthesis method and low in cost andis especially suitable for the modification of the urea-formaldehyde resin with low molar ratio.

The invention relates to application of a populus trichocarpa PtrbHLH186 gene in regulation and control of secondary xylem development of trees, and belongs to the technical field of gene engineering. In order to solve the problems that the need for wood quantity and quality is increased day by day and the biological function of the PtrbHLH186 transcription factor is unknown, a PtrbHLH186 overexpressed populus trichocarpa transgenic plant is obtained through an agrobacterium-mediated populus trichocarpa genetic transformation system, and then a series of technical means are utilized for analysis and discovery that the PtrbHLH186 overexpressed populus trichocarpa transgenic plant is found to have the advantages that the PtrbHLH186 overexpressed populus trichocarpa transgenic plant; the overexpression of the PtrbHLH186 transcription factor affects the growth and development of plants and the formation of wood, which is mainly embodied in that the lignin synthase gene is regulated by the gene to affect the deposition of lignin in the secondary cell wall of a tree, so that the morphological structure and distribution of xylem cells are changed; the results show that the PtrbHLH186 transcription factor has a certain regulation effect on the development of the secondary xylem of the tree. The research content provided by the invention has important theoretical guiding significance for cultivating new varieties of high-quality woods and forests.