53 results about "Lignin synthesis" patented technology

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention discloses a gene PbrSND1 for regulating and controlling synthesis of pear fruit lignin, the CDS sequence of the gene is as shown in SEQ ID NO.1, and the sequence of a coded protein is as shown in SEQ ID NO.2. Fluorescent quantitative analysis shows that the gene is mainly expressed in a key stage of development of stone cells of pear fruits. When an overexpression vector constructed by the gene is injected into a Dangshan pear fruit, the contents of stone cells, lignin and cellulose are obviously increased compared with those of injection of a no-load control part; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, secondary cell walls of conduit cells and intervascular bundle fiber cells of the obtained PbrSND1 transgenic arabidopsis thaliana are remarkably thickened, and accumulation of lignin and cellulose in stems is remarkably increased. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention relates to the technical field of gene engineering, and discloses a moso bamboo lignin synthesis related transcription factor PeLBT1 and application thereof. The amino acid sequence of the transcription factor PeLBT1 related to synthesis of moso bamboo lignin is as shown in SEQ ID NO. 1. The nucleotide sequence of the gene related to moso bamboo lignin synthesis is shown as SEQ ID NO.2, and the amino acid sequence coded by the gene is shown as SEQ ID NO.1. According to the moso bamboo lignin synthesis related transcription factor PeLBT1 and the application thereof, the transcription factor PeLBT1 or the coding gene thereof is overexpressed in rice, the lignin content in the rice can be effectively reduced, and a new gene resource is provided for plant wood property improvement gene engineering.

The invention discloses a method for synthesizing eutectic gel by in-situ extraction of lignin, and belongs to the technical field of gel materials.The method comprises the steps that choline chloride and acrylic acid are mixed, heated in an oil bath and stirred, and a eutectic solvent is obtained; cooling the solvent, adding dried and crushed wood powder, heating in an oil bath, extracting to obtain a mixed solution, and centrifuging to obtain supernate; adding a cross-linking agent and an initiator into the supernate, heating, stirring, and cooling to room temperature to obtain a pre-polymerized solution; and pouring the pre-polymerized liquid into a polytetrafluoroethylene mold, and carrying out ultraviolet curing to obtain the target gel. According to the method, the natural structure and active groups of the lignin are reserved to the maximum extent, the method is low-carbon, energy-saving, green and sustainable, in-situ lignin and a polymerization network cooperate, the gel is endowed with higher mechanical strength, environmental stability and functional diversity, and the limitation of existing lignin doped gel is broken through.

The invention discloses application of a pear transcription factor PbrMADS1 to regulation and control of synthesis of stone cells and lignin as a component of the stone cells. A transcription factor PbrMADS1 gene separated from Dangshan pears belongs to SEP subfamily members, the nucleotide sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.1, and the coded amino acid sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.2 in a sequence table. The lignin content and the lignin synthetic gene expression quantity of the pear pulp callus with the PbrMADS1 being knocked out are remarkably reduced. Therefore, it is proved that the PbrMADS1 gene regulates formation of the pear fruit stone cells and the lignin component of the pear fruit stone cells. And the PbrMADS1 gene has the advantage of being capable of regulating and controlling a plurality of genes at the same time, so that a more efficient way is provided for molecular breeding. The discovery of the gene supplements and perfects a pear stone cell character transcriptional regulation mechanism, and provides a theoretical basis and gene resources for improving the pear fruit stone cell content.

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

The invention discloses an application of proteins PeC4H1 and PeC4H2 or coding nucleic acids thereof in inhibition of lignin content of moso bamboo. The invention provides an application of a substance for inhibiting the activity of proteins PeC4H1 and PeC4H2 or inhibiting the expression of a nucleic acid molecule for encoding the protein PeC4H1 and a nucleic acid molecule for encoding the protein PeC4H2 in any one of the following C1-C4: C1) inhibiting the content of lignin in moso bamboo; c2) inhibiting accumulation of moso bamboo flavonoid substances; c3) cultivating moso bamboos with low lignin content; c4) cultivating moso bamboos with low flavonoid substances; according to the invention, PeC4H1 / 2 is knocked out through the double-gene CRISPR / Cas9, and a compensation mechanism of the double-gene CRISPR / Cas9 in lignin synthesis and carbon flow redirection is analyzed. Research results provide a double-target engineering strategy for optimizing the lignin content, and an innovative path is provided for deep fusion of bamboo breeding and a circulating biological economic principle.

The invention provides a method for increasing the synthesis amount of lignin in arabidopsis thaliana by using an agropyron cristatum AmCOMT gene, and belongs to the field of plant genetic engineering. The method comprises the following steps: transferring a nucleic acid molecule which is derived from Mongolian agropyron cristatum and is used for coding caffeic acid oxygen methyl transferase into arabidopsis thaliana, and enabling the arabidopsis thaliana to over-express the caffeic acid oxygen methyl transferase. According to the application, the COMT gene of the agropyron mongolicum is successfully transferred into the arabidopsis thaliana and is efficiently expressed in the arabidopsis thaliana, so that the transgenic arabidopsis thaliana containing the AmCOMT gene is obtained, the content of lignin in the cell wall of the arabidopsis thaliana is remarkably increased, and the stress resistance of the arabidopsis thaliana to drought, high-salt and other environments is remarkably enhanced.

The invention discloses a dendrocalamus latiflorus auxin response gene PeARF34 and application of the dendrocalamus latiflorus auxin response gene PeARF34 in regulating and controlling lignin content and plant type of bamboo. The nucleotide sequence of the dendrocalamus latiflorus auxin response gene PeARF34 is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein of the dendrocalamus latiflorus auxin response gene PeARF34 is shown as SEQ ID NO.2. The invention also discloses a PeARF34-SRDX protein obtained by fusing the protein and an SRDX peptide fragment, the amino acid sequence of the PeARF34-SRDX protein is shown as SEQ ID NO.4, and the nucleotide sequence of a gene for coding the protein is shown as SEQ ID NO.3. The invention also discloses a PeARF34-SRDX protein obtained by fusing the protein and the SRDX peptide fragment. Overexpression of PeARF34 in dendrocalamus latiflorus results in up-regulation of lignin synthetase gene expression and increase of lignin content. Overexpression of the PeARF34-SRDX leads to down-regulation of the lignin synthetase gene expression quantity and reduction of the lignin content. Therefore, the gene can realize directional regulation and control of the lignin content.

The invention discloses a method for improving wheat scab resistance by mutating a heavy metal binding protein TaHMAP. According to the invention, a homozygous three-mutant strain KO-1-3 of the TaHMAP gene is created, and the gibberellic disease resistance of a TaHMAP gene function deletion mutant is found to be improved. The study on the lignin synthesis pathway gene in the mutant finds that the expression of the lignin regulation gene is improved after the TaHMAP gene mutation. Lignin content determination shows that the lignin content of the homozygous three mutants of the TaHMAP gene is obviously higher than that of a wild plant. Therefore, the TaHMAP mutant strain has potential value in the aspects of analyzing a wheat gibberellic disease resistant signal channel and improving the resistance of wheat to gibberellic disease, and can be used as a genetic resource for wheat gibberellic disease resistant breeding.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor SbbHLH168 gene of sorghum in saline-alkali soil along a highway in regulating and controlling salt stress resistance of plants. The transcription factor SbbHLH168 of sorghum is researched, and it is revealed for the first time that the SbbHLH168 gene can improve the salt tolerance of plants. After salt stress treatment, compared with a control group, the root length of a transgenic plant subjected to gene overexpression is increased, the root system is increased, and the dry weight and the fresh weight are both increased; after salt stress treatment, compared with a root system part of a control plant, the Na < + > content of a transgenic plant subjected to gene overexpression is obviously lower, and the K < + > content is obviously higher; results show that the SbbHLH168 gene can promote the growth of a sorghum root system, reduce Na < + > accumulation in the root system, increase the K < + > content and improve the salt tolerance of sorghum. Meanwhile, the SbbHLH168 gene improves the salt resistance by regulating and controlling the expression of lignin synthesis related genes.

The present invention provides a lignin-based formaldehyde-free adhesive and a preparation method thereof. In the present invention, a turbid liquid is obtained by acid precipitation of black liquor, and the lignin concentrate obtained by centrifuging the turbid liquid can be directly used for synthesizing the formaldehyde-free adhesive without undergoing treatments such as spray drying or freeze drying, reducing the preparation cost; adding a diluent and a curing agent to the lignin concentrate can directly obtain the formaldehyde-free adhesive without modifying the lignin or synthesizing resins such as phenolic resin and urea-formaldehyde resin to obtain the adhesive, with a simple synthesis process and a simple structure, being suitable for large-scale production; for the adhesive of the present invention, the preparation raw materials do not contain formaldehyde or formaldehyde derivatives, and the adhesive obtained by this method has no risk of formaldehyde release, conforming to the concept of green chemistry; the adhesive obtained by this method has a stronger bonding strength and better performance compared with the adhesive synthesized from lignin resin.

The invention discloses an anti-lodging green high-yield cultivation method for marigold, and relates to the technical field of anti-lodging pesticides. The method comprises the following steps: soaking seeds for 15 minutes by mixing a 500-fold solution of 50% carbendazim wettable powder and a 1000-fold solution of a 10% difenoconazole suspending agent, mixing 30% of garden fallen leaf compost, 50% of humus soil and 20% of perlite according to a volume ratio, and adding 0.5% of charcoal. Wherein the plant growth regulator adopts a compound containing a deuterated group and a heterocyclic structure, and the breaking strength of stalks is enhanced by regulating lignin synthesis and hormone signal transduction. The anti-lodging green high-yield cultivation method for marigold has the characteristics of high efficiency, lodging resistance, yield increase, quality improvement and environmental friendliness, and solves the technical problems of serious pollution and short lasting effect of traditional chemical agents.

This invention relates to the field of genetic engineering technology, and discloses a transcription factor PeLBT1 related to lignin synthesis in moso bamboo and its applications. The amino acid sequence of PeLBT1 is shown in SEQ ID NO.1. The nucleotide sequence of a gene related to lignin synthesis in moso bamboo is shown in SEQ ID NO.2, and its encoded amino acid sequence is shown in SEQ ID NO.1. This invention utilizes the aforementioned transcription factor PeLBT1 and its applications to overexpress PeLBT1 or its encoding gene in rice, which can effectively reduce the lignin content in rice, providing a new gene resource for plant property improvement genetic engineering.

The invention discloses a nutrition regulation and control method for disease resistance of kiwi fruits, a germination fertilizer and a preparation method, and the preparation method comprises the following steps: constructing a ternary interaction system consisting of a sulfur source, succinic acid and Pseudomonas fluorescens, and applying the ternary interaction system to rhizosphere nutrition management of the kiwi fruits in early spring. And three-dimensional coupling of nutrition supply, signal activation and microbial regulation and control is synchronously realized in the rhizosphere microdomain. Meanwhile, lignin monomer synthesis is continuously activated and promoted through a salicylic acid signal channel, high-activity laccase serves as a key enzyme for lignin monomer polymerization, lignin monomer polymerization reaction is accelerated, synthesis and deposition of lignin in plant cell walls are promoted, plant endogenous disease-resistant signal cascade is continuously amplified, and plant disease resistance is improved. The kiwi fruits are induced to generate stable systematic disease resistance. The composition can prevent and control diseases for a long time and cooperatively promote plant growth.

The invention belongs to the technical field of agricultural biological genetic engineering, and particularly discloses application of a PbbHLH96 gene in regulation and control of pear fruit lignin synthesis and / or stone cell formation. According to the application of the PbHLH96 gene in regulation and control of lignin synthesis and / or stone cell formation of pear fruits, the nucleotide sequence of the PbHLH96 gene is as shown in SEQ ID NO. 1. The PbHLH96 gene is directly combined with a PbCAD6 promoter region to inhibit transcription of the PbHLH96 gene, so that biological synthesis of lignin of pear fruits is negatively regulated, and the PbHLH96 gene has a great application value for improving the quality of the pear fruits.

The invention belongs to the field of molecular genetic breeding, and particularly relates to application of a wheat AP2 transcription factor in cultivation of a wheat variety resistant to gibberellic disease. The invention finds that a wheat AP2 transcription factor regulates a wheat lignin synthesis pathway gene, and the expression of the lignin regulation gene after mutation is improved. The invention also creates a homozygous three-mutant strain AP2-KO of the AP2 transcription factor, and finds that in an AP2T1 strain in which AP2 is knocked out, the gibberellic disease resistance of a plant is remarkably improved, and the structure of a lignin monomer component is remarkably changed. Therefore, the AP2-KO strain has a potential value in analysis of wheat gibberellic disease resistance signal channels and improvement of wheat gibberellic disease resistance, and has a wide application prospect in the field of plant gibberellic disease resistance breeding.

The invention provides a preparation method of a biomass derived porous carbon material based on CRISPR editing, and relates to the technical field of preparation and application of porous carbon materials, the preparation method comprises the following steps: selecting a biomass raw material, the biomass raw material comprises lignin, the lignin content is greater than or equal to 15wt%, a CRISPR-Cas9 system is adopted to perform targeted editing on a key gene COMT synthesized by the lignin, and the lignin content is greater than or equal to 15wt%; and editing the COMT key gene by using a compound composed of sgRNA and Cas9 protein to obtain an edited biomass. According to the preparation method of the biomass-derived hierarchical porous carbon material based on CRISPR editing, a CRISPR-Cas9 system is adopted to carry out targeted editing on lignin synthesis key genes in biomass, and structure adjustment of lignin is successfully realized. And then, plasma cracking, MOF gas phase doping, pyrolysis, activation treatment and other process steps are combined to obtain the carbon material with the hierarchical pore structure, so that the specific surface area and the pore structure of the material are improved, and the performance of the material is optimized.

The application provides a transcription factor EjNAC5-like involved in lignin deposition and an application thereof, and the nucleotide sequence of the transcription factor EjNAC5-like is shown as SEQ ID NO. 1. The transcription factor EjNAC5-like can promote the expression of a key gene Ej4CL1 for monomer synthesis of pyracantha lignin and a polymerization gene EjPRX12 thereof, and further promote the accumulation of lignin. It is verified that the expression of the EjNAC5-like gene of the pyracantha fruit is positively correlated with the lignin content, the transient silencing of the EjNAC5-like gene can reduce the expression of the Ej4CL1 and EjPRX12 genes, and the lignin content of the pyracantha fruit is significantly reduced. The EjNAC5-like can be used as a candidate gene for pyracantha to carry out genetic engineering and improvement breeding, and is used for improving the texture quality of the fruit, and has potential application value.

The invention discloses a soybean GmUBC13 gene, an encoding protein thereof and application of the soybean GmUBC13 gene in negative regulation and control of lignin synthesis and phytophthora root rot resistance, and belongs to the technical field of biology, and the nucleotide sequence of the soybean GmUBC13 gene is shown as SEQ ID NO: 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO: 2. According to the invention, it is found for the first time that the soybean GmUBC13 gene can negatively regulate lignin biosynthesis and the resistance of soybean to phytophthora root rot, that is, the GmUBC13 gene and the encoded protein thereof are successfully cloned from soybean, and it is confirmed for the first time by systematic functional experiments that the gene encodes an active E2 ubiquitin coupling enzyme. The overexpression of the gene can significantly inhibit the biosynthesis of soybean lignin, and the interference of the expression of the gene can promote the accumulation of lignin, so that the blank about a key negative regulation element in a lignin synthesis regulation network is filled.

The present application relates to the field of biological agriculture, and in particular to the application of the gene MtUMAMIT8 in regulating the growth and development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, and the phenotype of the homozygous mutant NF8465 / NF12630 is analyzed, confirming that the down-regulation of the gene MtUMAMIT8 leads to the growth of Medicago truncatula being hindered but the lignin content being reduced, and that the gene MtUMAMIT8 positively regulates the growth and development of the plant height, branch number and leaf size of Medicago truncatula and negatively regulates the synthesis of lignin, thereby providing a new gene resource for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

The invention relates to a physical property improvement method, extraction method and application of poplar nanocellulose, the improvement method is to improve the physical property of the poplar nanocellulose by inhibiting the expression of lignin synthetic gene CSE, and the specific improvement is that the biomass is slightly reduced, the lignin content is reduced, and the crystallinity of the cellulose is slightly increased. And the length-diameter ratio of the nanocellulose is reduced, so that the mechanical property of the nanocellulose is enhanced. The extraction method comprises the following steps: preparing raw materials; removing lignin by using sodium chlorite; carrying out TEMPO (Tetramethylpiperidinol) mediated oxidation treatment; and defibrating and dispersing the nanofibers. The improved nanocellulose is applied to the field of nanofiber-based materials, particularly to preparation of aerogel and films, the compressive strength and elasticity of the prepared aerogel are enhanced, the ductility, tensile strength and breaking energy of the films are higher, and the material toughness is better. The invention provides new breakthrough and inspiration for the application of the nanocellulose, and clarifies the value of the CSE gene in the aspects of wood quality character improvement and nanofiber-based application.

This invention belongs to the field of forage crop cultivation, specifically relating to a high-yield forage crop cultivation method for herbivorous animal feed. This invention utilizes both inner and outer seed-encapsulated liquids to provide forage seeds with a sufficient nutritional environment, reducing abiotic stress during growth. Trichoderma and other microorganisms in the inner seed-encapsulated liquid reproduce and absorb nutrients from the outer seed-encapsulated liquid and the external soil environment, then supply their metabolites to the forage seeds. The improved water and fertilizer system of this invention enhances soil conditions. The compound preparation of this invention inhibits the key enzyme in lignin synthesis, 4-coumaric acid-coenzyme A ligase. Applying the compound preparation after harvesting allows for precise dosage control, preventing lodging and reducing lignin synthesis. Forage obtained using this method has lower lignin content, better palatability for animals, higher yield, and better quality, thus reducing input costs in the livestock industry.

The invention discloses a gene PbMYB20 for regulating and controlling synthesis of pear fruit lignin, the CDS sequence of the gene is as shown in SEQ ID NO.1, and the sequence of a coded protein is as shown in SEQ ID NO.2. When an overexpression vector constructed by the gene is injected into a Dangshan pear fruit, the content of stone cells and lignin is remarkably increased compared with that of injection of a no-load control part; when a silent vector constructed by the gene is injected into a Dangshan pear fruit, the stone cell content and the lignin content are obviously reduced compared with those of a no-load control part; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, the secondary cell wall of conduit cells of the obtained PbMYB20 transgenic arabidopsis thaliana is obviously thickened, and the accumulation of lignin in stems is obviously increased. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The invention discloses a method for improving rice blast resistance by mutation of an OsPi21L gene through a gene editing technology. According to the invention, a homozygous mutant strain of three OsPi21L genes is created. It is found that the rice blast resistance of the OsPi21L gene function deletion mutant is improved along with the improvement of the expression quantity of lignin synthesis related genes. Lignin content determination shows that the lignin content of the OsPi21L gene mutant is obviously higher than that of a wild plant. Therefore, the OsPi21L mutant strain has potential value in the aspects of analyzing the rice blast resistance signal channel and improving the rice blast resistance of the rice, and can be used as a genetic resource for rice blast resistance breeding of the rice.

The present invention discloses a gene for regulating lignin synthesis in pear fruit PbrSND1, The CDS sequence of this gene is shown in SEQ ID NO.1, and the encoded protein sequence is shown in SEQ ID NO.2. Fluorescence quantitative analysis showed that this gene is mainly expressed in the key stage of stone cell development in pear fruit. When the gene was overexpressed in the fruit of Dangshan Pear, the stone cell, lignin and cellulose contents were significantly increased compared with the empty control site. When the gene was overexpressed in Arabidopsis, the stone cell, lignin and cellulose contents were significantly increased. PbrSND1 The secondary cell walls of the vascular cells and intervascular fiber cells of transgenic Arabidopsis thaliana were significantly thickened, and the accumulation of lignin and cellulose in the stems was significantly increased. The discovery of this gene provides a new genetic resource for fruit quality breeding and is an important candidate gene for future genetic engineering to improve fruit quality breeding.

The invention relates to a PtoPASPA3A gene for regulating and controlling tree cell wall lignin synthesis and application of the PtoPASPA3A gene, and belongs to the technical field of gene engineering. The invention provides a PtoPASPA3A gene for regulating and controlling synthesis of lignin in a tree cell wall. The nucleotide sequence of the PtoPASPA3A gene is as shown in SEQ ID NO. 1. A populus tomentosa plant with an overexpressed PtoPASPA3A gene is obtained through an agrobacterium-mediated populus tomentosa genetic transformation system, and analysis finds that the overexpressed PtoPASPA3A gene does not influence the external growth phenotype of the plant, so that the xylem fiber cell secondary wall of the plant becomes thin, finally, the lignin content is reduced, the cell wall saccharification ability is improved, and the xylem fiber cell secondary wall becomes thin. It is shown that the PtoPASPA3A gene has the effect of regulating and controlling lignin on trees and has important theoretical guiding significance on cultivation of new varieties of paper pulp.

This invention belongs to the field of plant protection, specifically relating to the application of thiamine in the control of maize chlorotic mottle virus (MCMV). This invention elucidates the molecular mechanism by which thiamine enhances the host's antiviral activity through transcriptome sequencing. It was found that MCMV infection affects the transcriptional changes of genes related to the thiamine metabolic pathway, leading to the inhibition of endogenous thiamine biosynthesis in plants. After MCMV infection, the interaction between p31 and ZmTHIC alters the localization of ZmTHIC, thereby inhibiting thiamine synthesis. Exogenous application of thiamine enhances maize's resistance to MCMV. Thiamine inhibits MCMV infection by inducing the MAPK pathway to enhance plant defense responses and by inducing the phenylpropanone biosynthesis pathway to promote lignin synthesis in plant cell walls. Therefore, exogenous application of thiamine can promote maize growth, enhance plant defense responses, and increase lignin synthesis, thereby increasing resistance to viral diseases.