40 results about "Lignin synthesis" patented technology

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention discloses a method for synthesizing eutectic gel by in-situ extraction of lignin, and belongs to the technical field of gel materials.The method comprises the steps that choline chloride and acrylic acid are mixed, heated in an oil bath and stirred, and a eutectic solvent is obtained; cooling the solvent, adding dried and crushed wood powder, heating in an oil bath, extracting to obtain a mixed solution, and centrifuging to obtain supernate; adding a cross-linking agent and an initiator into the supernate, heating, stirring, and cooling to room temperature to obtain a pre-polymerized solution; and pouring the pre-polymerized liquid into a polytetrafluoroethylene mold, and carrying out ultraviolet curing to obtain the target gel. According to the method, the natural structure and active groups of the lignin are reserved to the maximum extent, the method is low-carbon, energy-saving, green and sustainable, in-situ lignin and a polymerization network cooperate, the gel is endowed with higher mechanical strength, environmental stability and functional diversity, and the limitation of existing lignin doped gel is broken through.

The invention discloses application of a pear transcription factor PbrMADS1 to regulation and control of synthesis of stone cells and lignin as a component of the stone cells. A transcription factor PbrMADS1 gene separated from Dangshan pears belongs to SEP subfamily members, the nucleotide sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.1, and the coded amino acid sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.2 in a sequence table. The lignin content and the lignin synthetic gene expression quantity of the pear pulp callus with the PbrMADS1 being knocked out are remarkably reduced. Therefore, it is proved that the PbrMADS1 gene regulates formation of the pear fruit stone cells and the lignin component of the pear fruit stone cells. And the PbrMADS1 gene has the advantage of being capable of regulating and controlling a plurality of genes at the same time, so that a more efficient way is provided for molecular breeding. The discovery of the gene supplements and perfects a pear stone cell character transcriptional regulation mechanism, and provides a theoretical basis and gene resources for improving the pear fruit stone cell content.

The invention relates to the field of biological agriculture, in particular to application of a gene MtUMAMIT8 to regulation and control of growth, development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, the phenotype of a homozygous mutant NF8465 / NF12630 is analyzed, it is proved that the growth of medicago truncatula is hindered but the lignin content is reduced due to down-regulation of the gene MtUMAMIT8, it is clear that the gene MtUMAMIT8 positively regulates the growth and development of medicago truncatula plant height, the branch number, the leaf size and the like and negatively regulates lignin synthesis, and the gene MtUMAMIT8 can be used for identifying the lignin content of the medicago truncatula. And a new gene resource is provided for regulating and controlling the growth, development and quality of forage grass and breeding forage grass varieties with low lignin content.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

The invention discloses a method for improving wheat scab resistance by mutating a heavy metal binding protein TaHMAP. According to the invention, a homozygous three-mutant strain KO-1-3 of the TaHMAP gene is created, and the gibberellic disease resistance of a TaHMAP gene function deletion mutant is found to be improved. The study on the lignin synthesis pathway gene in the mutant finds that the expression of the lignin regulation gene is improved after the TaHMAP gene mutation. Lignin content determination shows that the lignin content of the homozygous three mutants of the TaHMAP gene is obviously higher than that of a wild plant. Therefore, the TaHMAP mutant strain has potential value in the aspects of analyzing a wheat gibberellic disease resistant signal channel and improving the resistance of wheat to gibberellic disease, and can be used as a genetic resource for wheat gibberellic disease resistant breeding.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor SbbHLH168 gene of sorghum in saline-alkali soil along a highway in regulating and controlling salt stress resistance of plants. The transcription factor SbbHLH168 of sorghum is researched, and it is revealed for the first time that the SbbHLH168 gene can improve the salt tolerance of plants. After salt stress treatment, compared with a control group, the root length of a transgenic plant subjected to gene overexpression is increased, the root system is increased, and the dry weight and the fresh weight are both increased; after salt stress treatment, compared with a root system part of a control plant, the Na < + > content of a transgenic plant subjected to gene overexpression is obviously lower, and the K < + > content is obviously higher; results show that the SbbHLH168 gene can promote the growth of a sorghum root system, reduce Na < + > accumulation in the root system, increase the K < + > content and improve the salt tolerance of sorghum. Meanwhile, the SbbHLH168 gene improves the salt resistance by regulating and controlling the expression of lignin synthesis related genes.

The invention discloses an anti-lodging green high-yield cultivation method for marigold, and relates to the technical field of anti-lodging pesticides. The method comprises the following steps: soaking seeds for 15 minutes by mixing a 500-fold solution of 50% carbendazim wettable powder and a 1000-fold solution of a 10% difenoconazole suspending agent, mixing 30% of garden fallen leaf compost, 50% of humus soil and 20% of perlite according to a volume ratio, and adding 0.5% of charcoal. Wherein the plant growth regulator adopts a compound containing a deuterated group and a heterocyclic structure, and the breaking strength of stalks is enhanced by regulating lignin synthesis and hormone signal transduction. The anti-lodging green high-yield cultivation method for marigold has the characteristics of high efficiency, lodging resistance, yield increase, quality improvement and environmental friendliness, and solves the technical problems of serious pollution and short lasting effect of traditional chemical agents.

This invention relates to the field of genetic engineering technology, and discloses a transcription factor PeLBT1 related to lignin synthesis in moso bamboo and its applications. The amino acid sequence of PeLBT1 is shown in SEQ ID NO.1. The nucleotide sequence of a gene related to lignin synthesis in moso bamboo is shown in SEQ ID NO.2, and its encoded amino acid sequence is shown in SEQ ID NO.1. This invention utilizes the aforementioned transcription factor PeLBT1 and its applications to overexpress PeLBT1 or its encoding gene in rice, which can effectively reduce the lignin content in rice, providing a new gene resource for plant property improvement genetic engineering.

The invention discloses a nutrition regulation and control method for disease resistance of kiwi fruits, a germination fertilizer and a preparation method, and the preparation method comprises the following steps: constructing a ternary interaction system consisting of a sulfur source, succinic acid and Pseudomonas fluorescens, and applying the ternary interaction system to rhizosphere nutrition management of the kiwi fruits in early spring. And three-dimensional coupling of nutrition supply, signal activation and microbial regulation and control is synchronously realized in the rhizosphere microdomain. Meanwhile, lignin monomer synthesis is continuously activated and promoted through a salicylic acid signal channel, high-activity laccase serves as a key enzyme for lignin monomer polymerization, lignin monomer polymerization reaction is accelerated, synthesis and deposition of lignin in plant cell walls are promoted, plant endogenous disease-resistant signal cascade is continuously amplified, and plant disease resistance is improved. The kiwi fruits are induced to generate stable systematic disease resistance. The composition can prevent and control diseases for a long time and cooperatively promote plant growth.

The invention belongs to the technical field of agricultural biological genetic engineering, and particularly discloses application of a PbbHLH96 gene in regulation and control of pear fruit lignin synthesis and / or stone cell formation. According to the application of the PbHLH96 gene in regulation and control of lignin synthesis and / or stone cell formation of pear fruits, the nucleotide sequence of the PbHLH96 gene is as shown in SEQ ID NO. 1. The PbHLH96 gene is directly combined with a PbCAD6 promoter region to inhibit transcription of the PbHLH96 gene, so that biological synthesis of lignin of pear fruits is negatively regulated, and the PbHLH96 gene has a great application value for improving the quality of the pear fruits.

The invention belongs to the field of molecular genetic breeding, and particularly relates to application of a wheat AP2 transcription factor in cultivation of a wheat variety resistant to gibberellic disease. The invention finds that a wheat AP2 transcription factor regulates a wheat lignin synthesis pathway gene, and the expression of the lignin regulation gene after mutation is improved. The invention also creates a homozygous three-mutant strain AP2-KO of the AP2 transcription factor, and finds that in an AP2T1 strain in which AP2 is knocked out, the gibberellic disease resistance of a plant is remarkably improved, and the structure of a lignin monomer component is remarkably changed. Therefore, the AP2-KO strain has a potential value in analysis of wheat gibberellic disease resistance signal channels and improvement of wheat gibberellic disease resistance, and has a wide application prospect in the field of plant gibberellic disease resistance breeding.

The invention provides a preparation method of a biomass derived porous carbon material based on CRISPR editing, and relates to the technical field of preparation and application of porous carbon materials, the preparation method comprises the following steps: selecting a biomass raw material, the biomass raw material comprises lignin, the lignin content is greater than or equal to 15wt%, a CRISPR-Cas9 system is adopted to perform targeted editing on a key gene COMT synthesized by the lignin, and the lignin content is greater than or equal to 15wt%; and editing the COMT key gene by using a compound composed of sgRNA and Cas9 protein to obtain an edited biomass. According to the preparation method of the biomass-derived hierarchical porous carbon material based on CRISPR editing, a CRISPR-Cas9 system is adopted to carry out targeted editing on lignin synthesis key genes in biomass, and structure adjustment of lignin is successfully realized. And then, plasma cracking, MOF gas phase doping, pyrolysis, activation treatment and other process steps are combined to obtain the carbon material with the hierarchical pore structure, so that the specific surface area and the pore structure of the material are improved, and the performance of the material is optimized.

The application provides a transcription factor EjNAC5-like involved in lignin deposition and an application thereof, and the nucleotide sequence of the transcription factor EjNAC5-like is shown as SEQ ID NO. 1. The transcription factor EjNAC5-like can promote the expression of a key gene Ej4CL1 for monomer synthesis of pyracantha lignin and a polymerization gene EjPRX12 thereof, and further promote the accumulation of lignin. It is verified that the expression of the EjNAC5-like gene of the pyracantha fruit is positively correlated with the lignin content, the transient silencing of the EjNAC5-like gene can reduce the expression of the Ej4CL1 and EjPRX12 genes, and the lignin content of the pyracantha fruit is significantly reduced. The EjNAC5-like can be used as a candidate gene for pyracantha to carry out genetic engineering and improvement breeding, and is used for improving the texture quality of the fruit, and has potential application value.

The invention discloses a soybean GmUBC13 gene, an encoding protein thereof and application of the soybean GmUBC13 gene in negative regulation and control of lignin synthesis and phytophthora root rot resistance, and belongs to the technical field of biology, and the nucleotide sequence of the soybean GmUBC13 gene is shown as SEQ ID NO: 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO: 2. According to the invention, it is found for the first time that the soybean GmUBC13 gene can negatively regulate lignin biosynthesis and the resistance of soybean to phytophthora root rot, that is, the GmUBC13 gene and the encoded protein thereof are successfully cloned from soybean, and it is confirmed for the first time by systematic functional experiments that the gene encodes an active E2 ubiquitin coupling enzyme. The overexpression of the gene can significantly inhibit the biosynthesis of soybean lignin, and the interference of the expression of the gene can promote the accumulation of lignin, so that the blank about a key negative regulation element in a lignin synthesis regulation network is filled.

The present application relates to the field of biological agriculture, and in particular to the application of the gene MtUMAMIT8 in regulating the growth and development and quality of forage grass. The biological function of the gene MtUMAMIT8 is identified for the first time, the MtUMAMIT8 gene is screened and locked through multi-omics analysis, and the phenotype of the homozygous mutant NF8465 / NF12630 is analyzed, confirming that the down-regulation of the gene MtUMAMIT8 leads to the growth of Medicago truncatula being hindered but the lignin content being reduced, and that the gene MtUMAMIT8 positively regulates the growth and development of the plant height, branch number and leaf size of Medicago truncatula and negatively regulates the synthesis of lignin, thereby providing a new gene resource for regulating the growth and development and quality of forage grass and breeding forage grass varieties with low lignin content.

The invention relates to a physical property improvement method, extraction method and application of poplar nanocellulose, the improvement method is to improve the physical property of the poplar nanocellulose by inhibiting the expression of lignin synthetic gene CSE, and the specific improvement is that the biomass is slightly reduced, the lignin content is reduced, and the crystallinity of the cellulose is slightly increased. And the length-diameter ratio of the nanocellulose is reduced, so that the mechanical property of the nanocellulose is enhanced. The extraction method comprises the following steps: preparing raw materials; removing lignin by using sodium chlorite; carrying out TEMPO (Tetramethylpiperidinol) mediated oxidation treatment; and defibrating and dispersing the nanofibers. The improved nanocellulose is applied to the field of nanofiber-based materials, particularly to preparation of aerogel and films, the compressive strength and elasticity of the prepared aerogel are enhanced, the ductility, tensile strength and breaking energy of the films are higher, and the material toughness is better. The invention provides new breakthrough and inspiration for the application of the nanocellulose, and clarifies the value of the CSE gene in the aspects of wood quality character improvement and nanofiber-based application.

This invention belongs to the field of forage crop cultivation, specifically relating to a high-yield forage crop cultivation method for herbivorous animal feed. This invention utilizes both inner and outer seed-encapsulated liquids to provide forage seeds with a sufficient nutritional environment, reducing abiotic stress during growth. Trichoderma and other microorganisms in the inner seed-encapsulated liquid reproduce and absorb nutrients from the outer seed-encapsulated liquid and the external soil environment, then supply their metabolites to the forage seeds. The improved water and fertilizer system of this invention enhances soil conditions. The compound preparation of this invention inhibits the key enzyme in lignin synthesis, 4-coumaric acid-coenzyme A ligase. Applying the compound preparation after harvesting allows for precise dosage control, preventing lodging and reducing lignin synthesis. Forage obtained using this method has lower lignin content, better palatability for animals, higher yield, and better quality, thus reducing input costs in the livestock industry.

The invention discloses a gene PbMYB20 for regulating and controlling synthesis of pear fruit lignin, the CDS sequence of the gene is as shown in SEQ ID NO.1, and the sequence of a coded protein is as shown in SEQ ID NO.2. When an overexpression vector constructed by the gene is injected into a Dangshan pear fruit, the content of stone cells and lignin is remarkably increased compared with that of injection of a no-load control part; when a silent vector constructed by the gene is injected into a Dangshan pear fruit, the stone cell content and the lignin content are obviously reduced compared with those of a no-load control part; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, the secondary cell wall of conduit cells of the obtained PbMYB20 transgenic arabidopsis thaliana is obviously thickened, and the accumulation of lignin in stems is obviously increased. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The invention discloses a method for improving rice blast resistance by mutation of an OsPi21L gene through a gene editing technology. According to the invention, a homozygous mutant strain of three OsPi21L genes is created. It is found that the rice blast resistance of the OsPi21L gene function deletion mutant is improved along with the improvement of the expression quantity of lignin synthesis related genes. Lignin content determination shows that the lignin content of the OsPi21L gene mutant is obviously higher than that of a wild plant. Therefore, the OsPi21L mutant strain has potential value in the aspects of analyzing the rice blast resistance signal channel and improving the rice blast resistance of the rice, and can be used as a genetic resource for rice blast resistance breeding of the rice.

The invention relates to a PtoPASPA3A gene for regulating and controlling tree cell wall lignin synthesis and application of the PtoPASPA3A gene, and belongs to the technical field of gene engineering. The invention provides a PtoPASPA3A gene for regulating and controlling synthesis of lignin in a tree cell wall. The nucleotide sequence of the PtoPASPA3A gene is as shown in SEQ ID NO. 1. A populus tomentosa plant with an overexpressed PtoPASPA3A gene is obtained through an agrobacterium-mediated populus tomentosa genetic transformation system, and analysis finds that the overexpressed PtoPASPA3A gene does not influence the external growth phenotype of the plant, so that the xylem fiber cell secondary wall of the plant becomes thin, finally, the lignin content is reduced, the cell wall saccharification ability is improved, and the xylem fiber cell secondary wall becomes thin. It is shown that the PtoPASPA3A gene has the effect of regulating and controlling lignin on trees and has important theoretical guiding significance on cultivation of new varieties of paper pulp.

This invention belongs to the field of plant protection, specifically relating to the application of thiamine in the control of maize chlorotic mottle virus (MCMV). This invention elucidates the molecular mechanism by which thiamine enhances the host's antiviral activity through transcriptome sequencing. It was found that MCMV infection affects the transcriptional changes of genes related to the thiamine metabolic pathway, leading to the inhibition of endogenous thiamine biosynthesis in plants. After MCMV infection, the interaction between p31 and ZmTHIC alters the localization of ZmTHIC, thereby inhibiting thiamine synthesis. Exogenous application of thiamine enhances maize's resistance to MCMV. Thiamine inhibits MCMV infection by inducing the MAPK pathway to enhance plant defense responses and by inducing the phenylpropanone biosynthesis pathway to promote lignin synthesis in plant cell walls. Therefore, exogenous application of thiamine can promote maize growth, enhance plant defense responses, and increase lignin synthesis, thereby increasing resistance to viral diseases.

This invention discloses a method for increasing the lignin content in the roots of Lauraceae fruit trees, belonging to the field of plant physiological regulation technology. To increase the lignin content in the roots of Lauraceae fruit trees such as avocado trees, this invention employs the following techniques: application of a lignin-synthetic nutrient-enhancing solution; directional pulsed electric field treatment to enhance the permeability of the fruit tree's epidermal cell membranes; activation of key enzymes in phenylpropanoid metabolism by irradiation with specific wavelengths of ultraviolet light; and synergistic application of mechanical stress technology to stimulate and induce vascular tissue differentiation. By treating the roots of Lauraceae fruit trees, not only can the lignin content in the root system be increased by more than 30%, but the root system's stress resistance and mechanical strength can also be improved by more than 45%, while maintaining normal physiological metabolism of the plant. This provides a new solution for lodging-resistant cultivation and root rot prevention in Lauraceae fruit trees.

The invention relates to a method for cultivating low-antinutritional factor and high-nutrition medicago sativa and application, and belongs to the technical field of plant molecular biology. The method comprises the following steps: firstly, determining key targets for regulating lignin synthesis (COMT1), concanavalin synthesis (ARG10 gene) and plant type (MsCLE3a gene) in medicago sativa by utilizing genomics; then, a multi-target CRISPR-Cas9 gene editing carrier is constructed, and medicago sativa is transformed through an ultrasonic-assisted agrobacterium method; finally, an intelligent phenotypic platform integrating artificial intelligence and hyperspectral imaging is utilized, and excellent mutants with the content of lignin and concanavalin remarkably reduced and compact plant types are screened out in a high-throughput mode. The obtained new variety of medicago sativa is high in safety and high in nutrition density, and stem and leaf powder of the medicago sativa can be widely applied to food and nourishment industries as a super nutrition enhancer.

The invention discloses a method for increasing the lignin content of roots of lauraceae fruit trees, and belongs to the technical field of plant physiological regulation and control. In order to improve the content of lignin at the roots of the lauraceae fruit trees such as butyrospermum parkii, lignin is applied to synthesize nutrition enhancing liquid, the fruit tree epidermis cell membrane permeability is enhanced through directional pulsed electric field treatment, and phenylpropane metabolism key enzyme activity is activated through specific wave band ultraviolet irradiation; the root of the lauraceae fruit tree is treated, so that the lignin content of the root system can be increased by 30% or above, the stress resistance and mechanical strength of the root system can be increased by 45% or above, meanwhile, normal physiological metabolism of the plant is kept, and the survival rate of the lauraceae fruit tree is increased. And a new solution is provided for lodging-resistant cultivation and root rot prevention of the lauraceae fruit trees.

This invention belongs to the field of biological gene and peanut stalk strength technology, and specifically discloses a method for enhancing peanut stalk strength. AhWRKY41 This invention relates to genes and their applications. By using GWAS combined with bioinformatics to locate candidate genes, it ultimately identified genes in peanuts that can regulate stem strength. AhWRKY41 Genes; when AhWRKY41 After gene overexpression transformation of plants, compared with wild type, AhWRKY41 Gene overexpression lines exhibited higher stem strength, higher cellulose and lignin content, and increased expression of key genes in the lignin synthesis pathway; therefore, AhWRKY41 Gene overexpression can enhance the strength of plant stems.

The application discloses application of a moso bamboo PedARF23 gene in regulation of plant lignin synthesis and plant root development and belongs to the technical field of genetic engineering. The PedARF23 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. The present application can improve the lignin content, the length of a main root, the number of lateral roots and the length of lateral roots by improving the expression of PedARF23, which indicates that the gene can be applied in cultivation of high-lignin and / or developed-root plant varieties.

The utility model provides a kind of water-cooling reaction tank for lignin synthesis polycarboxylic retention agent, it is related to retention agent preparation technical field, including tank body and the stirring assembly being installed in the side of tank body, the water-cooled plate is arranged in the tank body, the fixed assembly is arranged in the water-cooled plate, the water-cooled plate is fixed in the tank body by fixed assembly, the tank body upper surface is equipped with cover plate, the plate heat exchanger is fixedly installed on the cover plate upper surface.The water-cooling reaction tank for lignin synthesis polycarboxylic retention agent is connected in parallel by being arranged in the water-cooled plate inside tree-shaped flow channel, its multistage branch can distribute water flow to greater area, effectively increase the contact area of cooling water and medium in reaction kettle, to improve overall heat transfer capacity, and, the end microtube network of multiple micro flow channels after branching can make that water flow distribution is more uniform, realize more uniform and fast heat extraction, avoid local overheating.

The invention provides application of a CsWRKY41 gene in regulation and control of plant lignin synthesis, and belongs to the technical field of plant genetic engineering. A plant overexpression vector and a gene silencing vector of the CsWRKY41 gene are constructed and are respectively subjected to genetic transformation in arabidopsis thaliana and a tea tree, and the result proves that the lignin content of a transgenic plant can be obviously improved by overexpressing the CsWRKY41 gene; on the contrary, silencing of the gene leads to reduction of lignin content. The invention discloses a molecular mechanism of the tea tree CsWRKY41 gene for enhancing plant drought resistance by positively regulating lignin biosynthesis for the first time. The gene and the related expression vector can be used for cultivating tea trees with high lignin content and strong drought resistance and even other new forest or crop varieties, and have wide application prospects in agricultural water conservation and stress resistance breeding.