47 results about "Phyllostachys edulis" patented technology

The invention relates to the technical field of remote sensing image recognition, in particular to a method for determining a moso bamboo forest snow disaster affected area based on a GEE platform. The method mainly solves the problems of low efficiency, high cost and difficulty in realizing large-range rapid evaluation caused by dependence on manual field investigation in the prior art. According to the technical scheme, the method comprises the following steps: firstly, obtaining a multi-temporal Sentinel-2 image before and after a snow disaster, and carrying out the preprocessing of the multi-temporal Sentinel-2 image; extracting spectral bands, vegetation indexes and texture features to form an initial feature set; then, a key feature variable combination is determined through statistical significance filtering and machine learning optimization; on this basis, establishing a logistic regression discrimination model and determining an optimal classification threshold; and finally, carrying out snow disaster state classification on the moso bamboo forest region by utilizing the trained model, and generating a disaster region spatial distribution diagram.

The invention belongs to the field of edible fungus culture, and particularly relates to a method for reducing disease occurrence and increasing the yield of dictyophora rubrovolvata. According to the method, before planting, a rotary cultivator is used for ploughing soil, wood vinegar is sprayed, temperature and humidity are controlled after hyphae are put on the soil, wood vinegar is sprayed in the growth stage of bamboo eggs, and temperature and humidity are controlled until harvesting, so that the occurrence of diseases is reduced, and the yield of dictyophora rubrovolvata is increased. The invention provides a method for reducing diseases and increasing the yield of dictyophora rubrovolvata through measures of improving the pH value of soil, preventing soil caking, stimulating rapid growth of hyphae, stimulating twisting of the hyphae, reducing diseases and insect pests and the like. By means of the method, use of pesticides in the dictyophora rubrovolvata planting process can be reduced, the yield of dictyophora rubrovolvata is increased, and dictyophora rubrovolvata diseases are reduced.

The invention discloses a preparation method of a moso bamboo derived hard carbon material and application of the moso bamboo derived hard carbon material in a sodium ion battery, and belongs to the technical field of new energy materials. Moso bamboo powder serves as a precursor, acid pickling pretreatment is conducted, then pre-carbonization is conducted at the temperature of 300-800 DEG C, secondary carbonization is conducted at the temperature of 1400-1500 DEG C after acid pickling purification, and the moso bamboo derived hard carbon material is obtained. And micropore closing and surface repairing are induced. By strictly limiting the technological process of low-temperature pre-carbonization, acid pickling and impurity removal and high-temperature reconstruction and hole closing, the contradiction that the biomass hard carbon material is large in specific surface area, low in first efficiency and insufficient in platform capacity is successfully solved. When being used as the negative electrode of the sodium ion battery, the composite material has the advantages of high reversible specific capacity (gt, 308mAh / g), high platform capacity ratio (gt, 53%) and high first charge-discharge efficiency (gt; the energy density and the cycling stability of the whole battery are remarkably improved, and a new way is provided for the development of a low-cost and high-performance sodium ion battery negative electrode material.

The invention belongs to the technical field of forestry information, and particularly relates to a moso bamboo forest individual tree high-precision segmentation method based on unmanned aerial vehicle and foundation LiDAR fusion. The method aims at solving the problems that in the prior art, in a complex bamboo forest scene, canopy information is incomplete, under-forest terrain and bamboo pole structure obtaining is inaccurate due to the fact that a data source is single, and over-segmentation and under-segmentation generally exist due to the fact that a moso bamboo growth mechanism is not considered in a traditional algorithm. Through multi-platform LiDAR data collaborative acquisition, point cloud accurate fusion is realized by adopting a registration algorithm with a bamboo pole vertical structure as a constraint, and a canopy-bamboo pole-terrain integrated three-dimensional model is formed. A comprehensive clustering algorithm fusing tree crown geometric attributes and a canopy growth space competition model is innovatively provided, the moso bamboo growth competition relation is quantified into dynamic constraint, the individual tree boundary is intelligently defined, the over-segmentation and under-segmentation problems in the segmentation process are effectively solved, and the moso bamboo forest individual tree parameter extraction precision and ecological rationality are remarkably improved.

The present application relates to the technical field of plant breeding, and in particular to a mutation breeding method for Phyllostachys edulis based on ARTP. The mutation breeding method comprises: using atmospheric room temperature plasma to treat the seeds of Phyllostachys edulis, and the parameters include: radio frequency power of 200-400 W, radiation distance of 2-3 mm, and treatment time of 8-55 minutes. The present application studies a mutation breeding method suitable for Phyllostachys edulis, and through a specific degree of ARTP mutation of the seeds of Phyllostachys edulis, the number of tillers of the seedlings of Phyllostachys edulis can be increased and the yellowing rate of the seedling of Phyllostachys edulis can be reduced under the condition of ensuring a certain seedling rate. The mutation breeding method provided by the present application can be used for breeding Phyllostachys edulis varieties with excellent traits, and has important value in the field of Phyllostachys edulis breeding.

The invention discloses a phyllostachys edulis PeAKIN3 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys pubescens PeAKIN3 gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence contained in the polypeptide coded by the phyllostachys pubescens PeAKIN3 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an overexpression vector of a phyllostachys edulis PeAKIN3 gene; the constructed overexpression vector of the phyllostachys edulis PeAKIN3 gene is transformed into arabidopsis thaliana; and culturing and screening to obtain the transgenic arabidopsis thaliana strain. The invention discloses the function of the PeAKIN3 gene for the first time, and compared with a control group, transgenic arabidopsis thaliana with overexpression of the phyllostachys edulis PeAKIN3 gene shows obvious late flower phenotype, the number of rosette leaves is increased, and the flowering time is delayed, which indicates that the PeAKIN3 can prolong the vegetative growth period of the plant and postpone the reproductive growth of the plant.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis PeMASR1 gene, biological material and application thereof. The PeMASR1 gene provided by the present application is a new gene discovered in the study of cold tolerance of Phyllostachys edulis leaves, the nucleotide sequence of which is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. Through gene cloning, expression and comparative analysis, the phylogenetic position of the Phyllostachys edulis PeMASR1 gene and the important role thereof in the research of plant response to abiotic stress mechanism and photosynthesis are revealed. Overexpression in Columbia-type Arabidopsis plants can make the rosette leaves of the plants early senesce; and the plants show a more stress-tolerant phenotype than wild-type Arabidopsis in response to cold stress, drought stress and salt stress and other abiotic stresses.

The invention belongs to the technical field of plant breeding, and particularly relates to an SSR molecular marker for distinguishing moso bamboo variants and application of the SSR molecular marker. According to the invention, 209 phyllostachys pubescens variant genome SSR molecular markers are screened out. The 209 phyllostachys pubescens variant genome SSR molecular markers and the polymorphic primers based on the molecular markers can be applied to phyllostachys pubescens variant SSR molecular marker development and DNA fingerprint spectrum construction, SSR marker site difference analysis can be performed on phyllostachys pubescens variants, a theoretical basis is provided for phyllostachys pubescens resource protection, utilization and identification, and the application prospect is broad. Different variants of the moso bamboos can be distinguished, and the lower grades of the moso bamboos can be Besides, the SSR molecular marker has the characteristics of good stability, high polymorphism and strong universality, and the SSR marker site disclosed by the invention has the advantages of convenience, rapidness and good sharing property when being used for analyzing the moso bamboo variant fingerprint spectrum.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.

This application relates to an ecological protection structure for rock slopes using bamboo anchors and its construction method. The protection structure includes: a rock slope with multiple holes on its surface; multiple hollow bamboo anchors, inserted one by one into each hole; the inner cavity of each bamboo anchor is filled with nutrient soil for planting; each bamboo anchor comprises two parts: a first part fixed in the hole, and a second part located outside the hole with an opening connecting to the inner cavity; a protective net that runs through the second part of the bamboo anchors and is laid on the surface of the rock slope; a substrate layer that covers the surface of the protective net, with its top surface above the opening; and plant seeds within the substrate layer. The bamboo anchors integrate anchoring and ecological functions. The nutrient soil filling and the opening connecting to the upper sprayed substrate layer provide a stable deep water and fertilizer supply for the plants, thereby improving the vegetation survival rate and coverage of the rock slope and achieving integrated support and ecological restoration.

The application discloses a bamboo straw preparation Phyllostachys edulis breaking device and a method thereof, and relates to the technical field of Phyllostachys edulis breaking. The device comprises a fixing column, a first mounting seat is arranged on the side of the fixing column close to a first mounting plate, the first mounting seat is fixedly connected to the outer surface of the fixing column, the number of the first mounting seats is circumferentially arranged as several, the inner walls of the several first mounting seats are all rotationally connected with bent rods through rotating rods, the ends, away from the first mounting seats, of the several bent rods are all provided with second mounting seats, the inner walls of the second mounting seats are rotationally connected with the bent rods through rotating rods, the outer surfaces of the several bent rods are fixedly connected with fixing rings, and the fixing rings are arranged outside the fixing column. The inner walls of the bamboo tubes are uniformly extruded through the arrangement of the several bent rods, so that the bamboo tubes are fixed outside the fixing rings.

This invention relates to the field of honeycomb structure technology, specifically to a biomimetic reinforced chiral honeycomb structure. The structure comprises multiple periodically arranged chiral honeycomb basic units, each consisting of a ring and multiple ligaments connecting the rings. At least some of the ligaments have circular biomimetic reinforcing nodes, the diameter of which is larger than the thickness of the ligament and is integrally connected to it. This invention simulates the localized reinforcement structure of the enlarged bamboo nodes in *Phyllostachys edulis* by locally setting biomimetic reinforcing nodes in the ligaments, significantly improving the bending stiffness and load-bearing capacity of the ligaments with relatively low added mass, resulting in higher plateau stress in the structure. This invention has a simple structural form, is relatively easy to manufacture, and can be extended to other bending-dominant honeycomb structures.

The invention discloses a phyllostachys edulis PeRD22 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys edulis PeRD22 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the phyllostachys edulis PeRD22 gene is as shown in SEQ ID NO.2. According to the invention, an overexpression vector of the PeRD22 gene is constructed and transformed into arabidopsis thaliana, and a transgenic plant of the PeRD22 gene is obtained through culture. The result shows that the flowering time of a PeRD22 gene overexpressed plant is delayed compared with that of a control plant, the number of rosette leaves is increased, and an obvious late flowering phenotype is shown, which indicates that the PeRD22 gene negatively regulates the flowering of the plant. The PeRD22 gene disclosed by the invention can be used for regulating and controlling the flowering phase of plants and is beneficial to breeding of high-quality bamboo plant varieties.

A Phyllostachys edulis-derived PheXTH2 protein for regulating a culm cell wall thickness of a plant, and an encoding gene and use thereof are provided. Through Agrobacterium-mediated transformation of wild-type Arabidopsis thaliana with a gene expression vector, overexpression of a PheXTH2 gene is found to increase cell wall thickness in interfascicular fibers. This result demonstrates that the gene exhibits a regulatory function in the culm cell wall thickness of a plant. Therefore, PheXTH2 serves as a potential molecular breeding tool for improving bamboo culm wood properties to enhance lumber yield and ecological benefits, indicating broad application prospects.

The invention discloses a Dictyophora rubrovolvata strain Jintoad No.1, which belongs to the field of edible mushrooms and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the strain is CCTCC NO: M20231095. The sporocarp stipe curtain of the strain Jintoad No.1 is pure white, the stipe is cylindrical and hollow, the shape of the stipe is large, and the commercial value is high. Secondly, the sporocarp of the strain is fleshy and compact, the stipe cutting hole chambers are more, the number of the stipe transverse cutting hole chambers is 3-4 layers, the taste is crisp, and the transportation is convenient; in addition, the time from sowing to first-crop fruiting and harvesting of the strain is only 60 days, and the cultivation period of the strain is obviously shorter than that of other existing dictyophora rubrovolvata strains; the strain also has the advantages of high yield, high selenium content and the like, the average yield of fresh mushrooms reaches 2104 kg / 667 m < 2 >, and the selenium content in sporocarp dried mushrooms reaches 0.345 mg / kg.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis anthocyanin reductase gene PeANR4, an extraction method and application thereof. The gene PeANR4 encodes an amino acid sequence as shown in SEQ ID NO:2. The present application first finds that the gene PeANR4 has the functions of promoting plant growth and improving drought resistance and salt resistance of plants. The Phyllostachys edulis anthocyanin reductase gene PeANR4 is introduced into Arabidopsis thaliana for verification, and it is found that the growth amount of the transgenic Arabidopsis thaliana plant is greatly increased, and the drought resistance and salt resistance of the plant are greatly improved compared with the wild Arabidopsis thaliana plant.

The application discloses a bamboo plasma membrane H + ATPase, its gene PePMHA9 and application, belongs to the technical field of genetic engineering, and particularly relates to a bamboo plasma membrane H + ATPase, a bamboo plasma membrane H + The amino acid sequence of the bamboo plasma membrane H ATPase is shown as SEQ ID NO. 1. A coding gene PePMHA9 of the bamboo plasma membrane H ATPase, and the nucleotide sequence of the coding gene PePMHA9 is shown as SEQ ID NO. 2. The bamboo plasma membrane H ATPase or the coding gene PePMHA9 is applied to promoting the growth of rice in a high light environment. The bamboo plasma membrane H + ATPase and its coding gene PePMHA9 are successfully separated and identified from bamboo, and are transferred into wild type rice through a transgenic technology to perform function verification, and the obtained transgenic rice has increased leaf stomatal aperture, improved photosynthetic capacity and increased biomass in the high light environment compared with the wild type rice.

The present application relates to the technical field of forestry disaster monitoring, in particular to a method for identifying Phyllostachys edulis forest snow disaster damage based on a differentiated point cloud encryption strategy; the present application reconstructs the three-dimensional structure of the bamboo forest by fitting the bamboo stem axis and image post-processing, identifies different types of damage and generates virtual point clouds to fill in the missing areas of the scan, effectively improving the accuracy of the bamboo stem axis; through hierarchical reconstruction and principal component analysis technology, the image expression of the bamboo stem and crown is enhanced, providing more detailed damage feature maps to help accurately assess the disaster situation. In addition, the targeted image processing based on the damage type further improves the accuracy and reliability of the damage analysis. Overall, the present application provides efficient and accurate technical support for post-disaster assessment and ecological restoration of bamboo forests.

The present application relates to the technical field of soil pollution treatment, in particular to a bamboo-based soda saline soil remediation agent and a preparation method thereof, the bamboo-based soda saline soil remediation agent is composed of the following components in parts by weight: 46-50 parts of mature bamboo-based organic matter, 31-36 parts of modified bamboo charcoal, 10-13 parts of potassium humate, 5-6 parts of desulfurized gypsum, and 1-3 parts of iron-zinc-manganese chelate; the mature bamboo-based organic matter is bamboo-based organic material treated by aerobic fermentation; the modified bamboo charcoal is obtained by modifying bamboo charcoal with citric acid and phytic acid, and has carboxyl and phosphoric acid functional groups grafted on the surface. The present application can significantly improve the physicochemical properties of soda saline soil, achieve the multiple goals of desalination, alkali reduction and soil fertilization by converting bamboo raw materials into mature bamboo-based organic matter and modified bamboo charcoal, and combining the synergistic effect of potassium humate, desulfurized gypsum and iron-zinc-manganese chelate.

The application discloses a Phyllostachys edulis PeSVP gene, a coding protein and application thereof, and belongs to the technical field of plant genetic engineering.The nucleotide sequence of the PeSVP gene disclosed by the application is shown as SEQ ID NO.1, and the amino acid sequence of the coding protein is shown as SEQ ID NO.2.The application constructs a PeSVP gene overexpression vector of Phyllostachys edulis, transforms the constructed PeSVP gene overexpression vector of Phyllostachys edulis into Arabidopsis thaliana, and cultivates, screens and obtains a transgenic Arabidopsis thaliana strain with delayed flowering time.The application discloses the function of the PeSVP gene for the first time, the transgenic Arabidopsis thaliana plant has delayed flowering time after the PeSVP gene is transformed into the Arabidopsis thaliana plant, shows an obvious late-flowering phenotype, and the number of rosette leaves is increased;the expression amount of a downstream regulation gene FT is significantly reduced.The application can be used in molecular breeding of plant flowering period regulation, and has important significance for cultivating new plant germplasm with higher biological yield.

The application relates to a bamboo shoot cleaning device, which comprises a workbench, a mounting groove is formed in the workbench, a conveyor is arranged in the mounting groove, and the conveyor is used for conveying bamboo shoots; a cleaning unit is arranged on the workbench and used for cleaning the bamboo shoots; the cleaning unit comprises a liquid supply part arranged at the bottom of the workbench, two spraying parts oppositely arranged on the workbench and located on the two sides of the mounting groove, and a brushing part arranged on the workbench and located above the conveyor; the liquid supply part is used for supplying liquid to the spraying parts; a shielding cover is arranged on the workbench; and a collecting bucket is arranged below the workbench. The bamboo shoots can be conveyed through cooperation of the conveyor and the cleaning unit, the bamboo shoots can sequentially pass through various cleaning links, manual carrying and operation are not needed, manual labor intensity is reduced, and cleaning efficiency is improved.

This invention discloses a method for simulating the wild cultivation of *Dictyophora indicum* using dead bamboo resources, comprising the following steps: digging trenches downwards in the soil layer between *Phyllostachys edulis* groves; placing a water collection layer at the bottom of the trench; laying a lower filling layer composed of chopped bamboo residue on top of the water collection layer; laying a middle filling layer composed of a mixture of chopped bamboo residue and humus on top of the lower filling layer; constructing a cultivation trough along the length of the trench on the middle filling layer for *Dictyophora indicum* cultivation; laying a layer of pre-prepared culture medium in the cultivation trough, then placing *Dictyophora indicum* spawn with multiple holes punched around its perimeter in the middle of the culture medium, and then evenly scattering the mycelium from inside the spawn evenly on the surface of the culture medium; covering the middle filling layer and culture medium with an upper filling layer composed of sandy loam; and finally covering the upper filling layer with a covering layer. This invention has high resource utilization, significant economic benefits, and obvious ecological benefits, achieving long-term moisture retention and nutrient supplementation, and enabling fruiting for many years.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a moso bamboo pectin acetyl esterase gene PePAE6 as well as an extraction method and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO:1. The gene has the functions of reducing the pectin acetylation level of plant cell walls, promoting pectin accumulation in plants, improving the photosynthetic capacity of plants, and promoting the growth of plants. The moso bamboo pectin acetyl esterase gene PePAE6 is introduced into rice for verification, and the transgenic rice plants have a lower pectin acetylation level of cell walls, a higher pectin accumulation amount, a stronger photosynthetic capacity of leaves, and a faster and better growth than wild-type rice plants.

The invention discloses phyllostachys pubescens plasma membrane H < + >-ATP enzyme as well as a gene PePMHA9 and application thereof, belongs to the technical field of gene engineering, and particularly relates to phyllostachys pubescens plasma membrane H < + >-ATP enzyme, and the amino acid sequence of the phyllostachys pubescens plasma membrane H < + >-ATP enzyme is shown as SEQ ID NO.1. The invention relates to a coding gene PePMHA9 of phyllostachys pubescens plasma membrane H < + >-ATP enzyme. The nucleotide sequence of the coding gene PePMHA9 is shown as SEQ ID NO. 2. The moso bamboo plasma membrane H < + >-ATP enzyme or the coding gene PePMHA9 is applied to promotion of rice growth in a highlight environment. The phyllostachys pubescens plasma membrane H < + >-ATPase and the coding gene PePMHA9 thereof are successfully separated and identified from phyllostachys pubescens, the phyllostachys pubescens plasma membrane H < + >-ATPase and the coding gene PePMHA9 are transferred into wild type rice through a transgenic technology for functional verification, and compared with the wild type rice, the obtained transgenic rice has the advantages that the leaf stomatal aperture is increased, the photosynthetic capacity is improved, and the biomass is increased in a highlight environment.

The application discloses a kind of based on size year's Phyllostachys edulis forest landscape differentiation feature evaluation method, comprising the following steps: obtaining regional boundary vector data and remote sensing image data;Based on remote sensing image data, obtain regional Phyllostachys edulis forest size year classification data and Phyllostachys edulis forest size year distribution area;Based on Phyllostachys edulis forest size year distribution area, create new type Phyllostachys edulis forest size year differentiation index;Based on Phyllostachys edulis forest size year differentiation index, the grade of Phyllostachys edulis forest size year differentiation is divided;Based on Phyllostachys edulis forest size year differentiation index, the landscape differentiation feature of Phyllostachys edulis forest of administrative division scale and pixel scale is comprehensively evaluated.The application proposes to carry out comprehensive evaluation on landscape features based on the size year differentiation degree of Phyllostachys edulis forest, compared with the prior art, the application creates new type Phyllostachys edulis forest size year differentiation index, enriches landscape analysis method, can multi-angle evaluation landscape feature, provides reference for local Phyllostachys edulis forest in size year management.

The invention discloses application of a phyllostachys edulis PeMTHFD1 gene in regulation and control of flowering and vegetative growth of plants, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the PeMTHFD1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoding protein of the PeMTHFD1 gene is as shown in SEQ ID NO. 2. The gene is constructed on an overexpression vector and transformed into arabidopsis thaliana, the obtained transgenic plant shows the phenotype of obvious flowering time postponing and rosette leaf number increasing, and the functions of the gene in the aspects of prolonging the vegetative growth period of the plant and postponing flowering are disclosed for the first time. The invention provides a new gene resource and an effective tool for regulating and controlling the plant growth period and improving the biomass through a genetic engineering means, and has an important application value in crop breeding.

The application discloses a kind of for non-destructive fast evaluation of bamboo physical mechanics property prediction model construction method, mainly including collecting the growth information of target bamboo and the positioning information of the sample site where it is located;The bamboo culm of target bamboo is determined for physical mechanics property, and the measured physical mechanics property data is obtained;Correlation analysis is carried out on growth information, positioning information and measured physical mechanics property data, and the index significantly related to physical mechanics property is selected as model input variable;Based on model input variable and measured physical mechanics property data, the prediction model for predicting the physical mechanics property of bamboo is constructed.The application introduces regional grouping effect and spatial autocorrelation structure when establishing the correlation model of bamboo culm growth character and bamboo physical mechanics property, so that the final prediction model can non-destructively determine the growth character of bamboo, quickly evaluate the physical performance of bamboo, and then greatly improve the grading utilization and high-value processing efficiency of different quality bamboo resources.

The invention provides a stachys sieboldii storage method, relates to the technical field of agricultural product storage, and solves the problems of cell damage, out-of-control of microorganisms and the like in the storage of stachys sieboldii through a synergistic strategy of pretreatment, impurity removal and drying in the shade, three-stage gradient cooling domestication, vitamin C + calcium chloride + chitosan improved soil burying, dynamic temperature and humidity regulation and control in the storage period and regular ventilation. According to the method, the cell rupture rate of the stachys sieboldii is reduced by 60%-65% compared with direct freezing, the moisture retention rate is 93.98%, the Vc degradation rate is 33.93%, the hardness retention rate is 89.61%, the rotting rate is only 1.22% when the stachys sieboldii is stored for 180 days, the shelf life is prolonged to 180 days or above, no chemical residues exist, and the method is suitable for large-scale storage and supports off-season sales and deep processing of the stachys sieboldii.

The invention discloses a moso bamboo PeMT2 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys edulis PeMT2 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoding protein of the phyllostachys edulis PeMT2 gene is as shown in SEQ ID NO.2. The preparation method comprises the following steps: cloning a phyllostachys pubescens PeMT2 gene to a pCAMBIA1302 plasmid, so as to obtain a recombinant vector pCAMBIA1302-PeMT2; transforming the recombinant vector into arabidopsis thaliana, and culturing and screening to obtain a transgenic arabidopsis thaliana strain. The transgenic arabidopsis strain shows an obvious late flowering phenotype, the number of rosette leaves is increased, and the flowering time is delayed. The invention discloses the function of the PeMT2 gene for the first time, and also discloses the application of the PeMT2 gene in regulating the flowering time of plants and the quantity of rosette leaves.

The invention discloses a phyllostachys pubescens flavone synthase I gene PeFNSI1, an encoded protein and an application of the phyllostachys pubescens flavone synthase I gene PeFNSI1, belongs to the technical field of genetic engineering, and particularly relates to the phyllostachys pubescens flavone synthase I gene PeFNSI1, the nucleotide sequence of the phyllostachys pubescens flavone synthase I gene PeFNSI1 is shown as SEQ ID NO.1, and the application of the phyllostachys pubescens flavone synthase I gene PeFNSI1 in enhancing The invention reveals for the first time that flavone synthetase I (PeFNSI) coded by the phyllostachys pubescens PeFNSI1 gene can specifically catalyze flavone precursor naringenin to be converted into apigenin and catalyze eriodictyol to be converted into luteolin, and can activate a'bifunctional 'regulation element of a flavone synthesis and salicylic acid defense pathway at the same time by reprogramming salicylic acid metabolic flux, so that the flavone synthetase I (PeFNSI) can be used for preparing the apigenin and the luteolin. The multi-element synergistic effect of flavone synthesis, salicylic acid metabolic flux regulation and control and disease resistance enhancement is achieved, the limitation of an existing disease resistance strategy is broken through, and a brand new molecular tool is provided for plant disease resistance breeding.