62 results about "Phyllostachys edulis" patented technology

The invention relates to the technical field of remote sensing image recognition, in particular to a method for determining a moso bamboo forest snow disaster affected area based on a GEE platform. The method mainly solves the problems of low efficiency, high cost and difficulty in realizing large-range rapid evaluation caused by dependence on manual field investigation in the prior art. According to the technical scheme, the method comprises the following steps: firstly, obtaining a multi-temporal Sentinel-2 image before and after a snow disaster, and carrying out the preprocessing of the multi-temporal Sentinel-2 image; extracting spectral bands, vegetation indexes and texture features to form an initial feature set; then, a key feature variable combination is determined through statistical significance filtering and machine learning optimization; on this basis, establishing a logistic regression discrimination model and determining an optimal classification threshold; and finally, carrying out snow disaster state classification on the moso bamboo forest region by utilizing the trained model, and generating a disaster region spatial distribution diagram.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention provides a method for repairing degraded moso bamboo forest soil. The method comprises the following steps: selecting a degraded moso bamboo forest; the moso bamboo forest is divided into a plurality of strip-shaped areas according to a high-density-low-density classification mode, and high-density areas and low-density areas which are distributed alternately are formed; the moso bamboo forest is subjected to strip-shaped thinning treatment, strip-shaped areas are divided according to a high-density-low-density classification mode, and moso bamboos of different growth ages are subjected to thinning according to a certain proportion; performing soil preparation treatment on the moso bamboo forest after thinning; planting nitrogen-fixing plants in the soil under the moso bamboo forest; and treating the soil under the moso bamboo forest by using a soil conditioner. According to the method for restoring the degraded moso bamboo forest soil, arbor layer-under-forest-soil integrated restoration is achieved, systematic improvement is conducted on multiple links of thinning, nitrogen fixation and fertilization, the content of N in the soil and the content of organic matter in the soil are increased, and the diameter at breast height of new bamboo is increased.

The invention belongs to the field of edible fungus culture, and particularly relates to a method for reducing disease occurrence and increasing the yield of dictyophora rubrovolvata. According to the method, before planting, a rotary cultivator is used for ploughing soil, wood vinegar is sprayed, temperature and humidity are controlled after hyphae are put on the soil, wood vinegar is sprayed in the growth stage of bamboo eggs, and temperature and humidity are controlled until harvesting, so that the occurrence of diseases is reduced, and the yield of dictyophora rubrovolvata is increased. The invention provides a method for reducing diseases and increasing the yield of dictyophora rubrovolvata through measures of improving the pH value of soil, preventing soil caking, stimulating rapid growth of hyphae, stimulating twisting of the hyphae, reducing diseases and insect pests and the like. By means of the method, use of pesticides in the dictyophora rubrovolvata planting process can be reduced, the yield of dictyophora rubrovolvata is increased, and dictyophora rubrovolvata diseases are reduced.

The invention discloses a preparation method of a moso bamboo derived hard carbon material and application of the moso bamboo derived hard carbon material in a sodium ion battery, and belongs to the technical field of new energy materials. Moso bamboo powder serves as a precursor, acid pickling pretreatment is conducted, then pre-carbonization is conducted at the temperature of 300-800 DEG C, secondary carbonization is conducted at the temperature of 1400-1500 DEG C after acid pickling purification, and the moso bamboo derived hard carbon material is obtained. And micropore closing and surface repairing are induced. By strictly limiting the technological process of low-temperature pre-carbonization, acid pickling and impurity removal and high-temperature reconstruction and hole closing, the contradiction that the biomass hard carbon material is large in specific surface area, low in first efficiency and insufficient in platform capacity is successfully solved. When being used as the negative electrode of the sodium ion battery, the composite material has the advantages of high reversible specific capacity (gt, 308mAh / g), high platform capacity ratio (gt, 53%) and high first charge-discharge efficiency (gt; the energy density and the cycling stability of the whole battery are remarkably improved, and a new way is provided for the development of a low-cost and high-performance sodium ion battery negative electrode material.

The invention belongs to the technical field of forestry information, and particularly relates to a moso bamboo forest individual tree high-precision segmentation method based on unmanned aerial vehicle and foundation LiDAR fusion. The method aims at solving the problems that in the prior art, in a complex bamboo forest scene, canopy information is incomplete, under-forest terrain and bamboo pole structure obtaining is inaccurate due to the fact that a data source is single, and over-segmentation and under-segmentation generally exist due to the fact that a moso bamboo growth mechanism is not considered in a traditional algorithm. Through multi-platform LiDAR data collaborative acquisition, point cloud accurate fusion is realized by adopting a registration algorithm with a bamboo pole vertical structure as a constraint, and a canopy-bamboo pole-terrain integrated three-dimensional model is formed. A comprehensive clustering algorithm fusing tree crown geometric attributes and a canopy growth space competition model is innovatively provided, the moso bamboo growth competition relation is quantified into dynamic constraint, the individual tree boundary is intelligently defined, the over-segmentation and under-segmentation problems in the segmentation process are effectively solved, and the moso bamboo forest individual tree parameter extraction precision and ecological rationality are remarkably improved.

The present application relates to the technical field of plant breeding, and in particular to a mutation breeding method for Phyllostachys edulis based on ARTP. The mutation breeding method comprises: using atmospheric room temperature plasma to treat the seeds of Phyllostachys edulis, and the parameters include: radio frequency power of 200-400 W, radiation distance of 2-3 mm, and treatment time of 8-55 minutes. The present application studies a mutation breeding method suitable for Phyllostachys edulis, and through a specific degree of ARTP mutation of the seeds of Phyllostachys edulis, the number of tillers of the seedlings of Phyllostachys edulis can be increased and the yellowing rate of the seedling of Phyllostachys edulis can be reduced under the condition of ensuring a certain seedling rate. The mutation breeding method provided by the present application can be used for breeding Phyllostachys edulis varieties with excellent traits, and has important value in the field of Phyllostachys edulis breeding.

The invention discloses a phyllostachys edulis PeAKIN3 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys pubescens PeAKIN3 gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence contained in the polypeptide coded by the phyllostachys pubescens PeAKIN3 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an overexpression vector of a phyllostachys edulis PeAKIN3 gene; the constructed overexpression vector of the phyllostachys edulis PeAKIN3 gene is transformed into arabidopsis thaliana; and culturing and screening to obtain the transgenic arabidopsis thaliana strain. The invention discloses the function of the PeAKIN3 gene for the first time, and compared with a control group, transgenic arabidopsis thaliana with overexpression of the phyllostachys edulis PeAKIN3 gene shows obvious late flower phenotype, the number of rosette leaves is increased, and the flowering time is delayed, which indicates that the PeAKIN3 can prolong the vegetative growth period of the plant and postpone the reproductive growth of the plant.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis PeMASR1 gene, biological material and application thereof. The PeMASR1 gene provided by the present application is a new gene discovered in the study of cold tolerance of Phyllostachys edulis leaves, the nucleotide sequence of which is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. Through gene cloning, expression and comparative analysis, the phylogenetic position of the Phyllostachys edulis PeMASR1 gene and the important role thereof in the research of plant response to abiotic stress mechanism and photosynthesis are revealed. Overexpression in Columbia-type Arabidopsis plants can make the rosette leaves of the plants early senesce; and the plants show a more stress-tolerant phenotype than wild-type Arabidopsis in response to cold stress, drought stress and salt stress and other abiotic stresses.

The invention discloses an application of proteins PeC4H1 and PeC4H2 or coding nucleic acids thereof in inhibition of lignin content of moso bamboo. The invention provides an application of a substance for inhibiting the activity of proteins PeC4H1 and PeC4H2 or inhibiting the expression of a nucleic acid molecule for encoding the protein PeC4H1 and a nucleic acid molecule for encoding the protein PeC4H2 in any one of the following C1-C4: C1) inhibiting the content of lignin in moso bamboo; c2) inhibiting accumulation of moso bamboo flavonoid substances; c3) cultivating moso bamboos with low lignin content; c4) cultivating moso bamboos with low flavonoid substances; according to the invention, PeC4H1 / 2 is knocked out through the double-gene CRISPR / Cas9, and a compensation mechanism of the double-gene CRISPR / Cas9 in lignin synthesis and carbon flow redirection is analyzed. Research results provide a double-target engineering strategy for optimizing the lignin content, and an innovative path is provided for deep fusion of bamboo breeding and a circulating biological economic principle.

The invention belongs to the technical field of plant molecular biology. Specifically, the invention relates to a moso bamboo stalk elongation regulation and control gene PeGPR10 and application of the moso bamboo stalk elongation regulation and control gene PeGPR10. The invention provides a moso bamboo stalk elongation regulation gene PeGPR10. The nucleotide sequence (CDS coding sequence) of the moso bamboo stalk elongation regulation gene PeGPR10 is shown as SEQ ID No: 1. The invention also provides application of the gene, namely reverse regulation of internode length of rice and reverse regulation of plant height of rice. Namely, compared with wild rice Nipponbare, the internode length of the transgenic rice is reduced, and the plant height is reduced.

The present invention discloses a Dendrocalamus farinosus transcription factor DfMYB265 gene and its application. Its nucleotide sequence is shown as SEQ ID NO.1, and the encoded amino acid sequence is shown as SEQ ID NO.2. In the present invention, the DfMYB265 gene is recombined with the pCAMBIA1303-N vector to form an expression vector, and then the embryos of Phyllostachys edulis seeds are genetically transformed by gene gun. Through transgenic positive identification and screening, Phyllostachys edulis plants overexpressing the DfMYB265 gene are obtained. Compared with wild-type Phyllostachys edulis, the Phyllostachys edulis plants overexpressing the DfMYB265 gene grow rapidly, with an increase in the number of branches and the number of bamboo shoots, an increase in plant height, and a significant increase in the cellulose content in the culm. The expression of cellulose synthase CESA4, CESA7, and CESA9 related to cellulose synthesis is significantly up-regulated. The present invention provides a theoretical basis for revealing the regulatory mechanism of DfMYB265 on cellulose in the process of secondary cell wall formation, provides ideas and a theoretical basis for improving the genetic transformation varieties of Dendrocalamus farinosus, and provides an effective way for the creation of new germplasms of bamboo plants and the increase of cellulose content.

The invention belongs to the technical field of plant breeding, and particularly relates to an SSR molecular marker for distinguishing moso bamboo variants and application of the SSR molecular marker. According to the invention, 209 phyllostachys pubescens variant genome SSR molecular markers are screened out. The 209 phyllostachys pubescens variant genome SSR molecular markers and the polymorphic primers based on the molecular markers can be applied to phyllostachys pubescens variant SSR molecular marker development and DNA fingerprint spectrum construction, SSR marker site difference analysis can be performed on phyllostachys pubescens variants, a theoretical basis is provided for phyllostachys pubescens resource protection, utilization and identification, and the application prospect is broad. Different variants of the moso bamboos can be distinguished, and the lower grades of the moso bamboos can be Besides, the SSR molecular marker has the characteristics of good stability, high polymorphism and strong universality, and the SSR marker site disclosed by the invention has the advantages of convenience, rapidness and good sharing property when being used for analyzing the moso bamboo variant fingerprint spectrum.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.

This application relates to an ecological protection structure for rock slopes using bamboo anchors and its construction method. The protection structure includes: a rock slope with multiple holes on its surface; multiple hollow bamboo anchors, inserted one by one into each hole; the inner cavity of each bamboo anchor is filled with nutrient soil for planting; each bamboo anchor comprises two parts: a first part fixed in the hole, and a second part located outside the hole with an opening connecting to the inner cavity; a protective net that runs through the second part of the bamboo anchors and is laid on the surface of the rock slope; a substrate layer that covers the surface of the protective net, with its top surface above the opening; and plant seeds within the substrate layer. The bamboo anchors integrate anchoring and ecological functions. The nutrient soil filling and the opening connecting to the upper sprayed substrate layer provide a stable deep water and fertilizer supply for the plants, thereby improving the vegetation survival rate and coverage of the rock slope and achieving integrated support and ecological restoration.

The application discloses a bamboo straw preparation Phyllostachys edulis breaking device and a method thereof, and relates to the technical field of Phyllostachys edulis breaking. The device comprises a fixing column, a first mounting seat is arranged on the side of the fixing column close to a first mounting plate, the first mounting seat is fixedly connected to the outer surface of the fixing column, the number of the first mounting seats is circumferentially arranged as several, the inner walls of the several first mounting seats are all rotationally connected with bent rods through rotating rods, the ends, away from the first mounting seats, of the several bent rods are all provided with second mounting seats, the inner walls of the second mounting seats are rotationally connected with the bent rods through rotating rods, the outer surfaces of the several bent rods are fixedly connected with fixing rings, and the fixing rings are arranged outside the fixing column. The inner walls of the bamboo tubes are uniformly extruded through the arrangement of the several bent rods, so that the bamboo tubes are fixed outside the fixing rings.

This invention relates to the field of honeycomb structure technology, specifically to a biomimetic reinforced chiral honeycomb structure. The structure comprises multiple periodically arranged chiral honeycomb basic units, each consisting of a ring and multiple ligaments connecting the rings. At least some of the ligaments have circular biomimetic reinforcing nodes, the diameter of which is larger than the thickness of the ligament and is integrally connected to it. This invention simulates the localized reinforcement structure of the enlarged bamboo nodes in *Phyllostachys edulis* by locally setting biomimetic reinforcing nodes in the ligaments, significantly improving the bending stiffness and load-bearing capacity of the ligaments with relatively low added mass, resulting in higher plateau stress in the structure. This invention has a simple structural form, is relatively easy to manufacture, and can be extended to other bending-dominant honeycomb structures.

The invention discloses a phyllostachys edulis PeRD22 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys edulis PeRD22 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the phyllostachys edulis PeRD22 gene is as shown in SEQ ID NO.2. According to the invention, an overexpression vector of the PeRD22 gene is constructed and transformed into arabidopsis thaliana, and a transgenic plant of the PeRD22 gene is obtained through culture. The result shows that the flowering time of a PeRD22 gene overexpressed plant is delayed compared with that of a control plant, the number of rosette leaves is increased, and an obvious late flowering phenotype is shown, which indicates that the PeRD22 gene negatively regulates the flowering of the plant. The PeRD22 gene disclosed by the invention can be used for regulating and controlling the flowering phase of plants and is beneficial to breeding of high-quality bamboo plant varieties.

The application discloses a Phyllostachys edulis PeBBX19 gene, a coding protein and application thereof, and belongs to the technical field of plant genetic engineering.The nucleotide sequence of the Phyllostachys edulis PeBBX19 gene disclosed by the application is shown as SEQ ID NO.1, and the amino acid sequence of the coding protein is shown as SEQ ID NO.2.The application constructs an overexpression vector of the Phyllostachys edulis PeBBX19 gene, transforms the constructed overexpression vector of the Phyllostachys edulis PeBBX19 gene into Arabidopsis, and cultivates, screens and obtains a transgenic Arabidopsis strain with delayed flowering time.The application discloses the function of the PeBBX19 gene for the first time, the transgenic Arabidopsis plant has delayed flowering time after the PeBBX19 gene is transformed into the Arabidopsis plant, obviously shows a late flowering phenotype, and the number of rosette leaves is increased; and the expression amount of a downstream regulation gene FT is significantly reduced.

This invention belongs to the field of plant cultivation technology, specifically relating to a tissue culture medium and method for *Phyllostachys edulis*. The tissue culture medium for *Phyllostachys edulis* of this invention includes an induction medium, a callus differentiation medium, a shoot-strengthening medium, and a rooting medium. The 6-BA, NAA, and carbon nanotubes in each medium work together to induce callus formation and differentiation in *Phyllostachys edulis*, resulting in a large number of clustered shoots and tissue culture seedlings, thereby obtaining uniform and robust plants.

A Phyllostachys edulis-derived PheXTH2 protein for regulating a culm cell wall thickness of a plant, and an encoding gene and use thereof are provided. Through Agrobacterium-mediated transformation of wild-type Arabidopsis thaliana with a gene expression vector, overexpression of a PheXTH2 gene is found to increase cell wall thickness in interfascicular fibers. This result demonstrates that the gene exhibits a regulatory function in the culm cell wall thickness of a plant. Therefore, PheXTH2 serves as a potential molecular breeding tool for improving bamboo culm wood properties to enhance lumber yield and ecological benefits, indicating broad application prospects.

The invention discloses a Dictyophora rubrovolvata strain Jintoad No.1, which belongs to the field of edible mushrooms and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the strain is CCTCC NO: M20231095. The sporocarp stipe curtain of the strain Jintoad No.1 is pure white, the stipe is cylindrical and hollow, the shape of the stipe is large, and the commercial value is high. Secondly, the sporocarp of the strain is fleshy and compact, the stipe cutting hole chambers are more, the number of the stipe transverse cutting hole chambers is 3-4 layers, the taste is crisp, and the transportation is convenient; in addition, the time from sowing to first-crop fruiting and harvesting of the strain is only 60 days, and the cultivation period of the strain is obviously shorter than that of other existing dictyophora rubrovolvata strains; the strain also has the advantages of high yield, high selenium content and the like, the average yield of fresh mushrooms reaches 2104 kg / 667 m < 2 >, and the selenium content in sporocarp dried mushrooms reaches 0.345 mg / kg.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis anthocyanin reductase gene PeANR4, an extraction method and application thereof. The gene PeANR4 encodes an amino acid sequence as shown in SEQ ID NO:2. The present application first finds that the gene PeANR4 has the functions of promoting plant growth and improving drought resistance and salt resistance of plants. The Phyllostachys edulis anthocyanin reductase gene PeANR4 is introduced into Arabidopsis thaliana for verification, and it is found that the growth amount of the transgenic Arabidopsis thaliana plant is greatly increased, and the drought resistance and salt resistance of the plant are greatly improved compared with the wild Arabidopsis thaliana plant.

The application discloses a bamboo plasma membrane H + ATPase, its gene PePMHA9 and application, belongs to the technical field of genetic engineering, and particularly relates to a bamboo plasma membrane H + ATPase, a bamboo plasma membrane H + The amino acid sequence of the bamboo plasma membrane H ATPase is shown as SEQ ID NO. 1. A coding gene PePMHA9 of the bamboo plasma membrane H ATPase, and the nucleotide sequence of the coding gene PePMHA9 is shown as SEQ ID NO. 2. The bamboo plasma membrane H ATPase or the coding gene PePMHA9 is applied to promoting the growth of rice in a high light environment. The bamboo plasma membrane H + ATPase and its coding gene PePMHA9 are successfully separated and identified from bamboo, and are transferred into wild type rice through a transgenic technology to perform function verification, and the obtained transgenic rice has increased leaf stomatal aperture, improved photosynthetic capacity and increased biomass in the high light environment compared with the wild type rice.

The present application relates to the technical field of forestry disaster monitoring, in particular to a method for identifying Phyllostachys edulis forest snow disaster damage based on a differentiated point cloud encryption strategy; the present application reconstructs the three-dimensional structure of the bamboo forest by fitting the bamboo stem axis and image post-processing, identifies different types of damage and generates virtual point clouds to fill in the missing areas of the scan, effectively improving the accuracy of the bamboo stem axis; through hierarchical reconstruction and principal component analysis technology, the image expression of the bamboo stem and crown is enhanced, providing more detailed damage feature maps to help accurately assess the disaster situation. In addition, the targeted image processing based on the damage type further improves the accuracy and reliability of the damage analysis. Overall, the present application provides efficient and accurate technical support for post-disaster assessment and ecological restoration of bamboo forests.

The invention discloses a panda staple food phyllostachys edulis microorganism obtaining method, and belongs to the technical field of microorganism identification. Comprising the following steps: S1, sampling; s2, microorganism enrichment: putting the bamboo leaf sample into a sterile plastic package bag, and adding a buffer solution to carry out vortex and ultrasonic treatment on the sample. After washing is finished, the bamboo leaves are taken out, liquid in the sterile plastic package bag is centrifuged, supernatant is discarded, thalli are collected, then a buffer solution is added for resuspending the thalli, then secondary centrifugation is carried out, finally supernatant is removed, resuspending and centrifuging are carried out again, bacterial sludge is collected, and cryopreservation is carried out at the temperature of-80 DEG C for standby application. S3, microbial culture: taking the bacterial sludge in the step S2, and culturing in a manner of combining solid culture plate culture and liquid shaking culture; bamboo leaf lixivium, plant hormone analogues and the like are added into the solid culture medium and the liquid culture medium; s4, microbial identification. According to the method, comprehensive analysis of phyllostachys edulis microorganisms from an original sample to a culture flora is realized, and the community diversity covered by an identification result is ensured.

The invention discloses a moso bamboo-derived gene and application of encoded protein thereof, and belongs to the technical field of gene engineering. The gene derived from the moso bamboo comprises a PeDHN4 gene and a PeDHN8 gene; the nucleotide sequence of the PeDHN4 gene is as shown in SEQ ID NO. 15; the nucleotide sequence of the PeDHN8 gene is as shown in SEQ ID NO. 17. According to the PeDHN4 gene and the PeDHN8 gene cloned from moso bamboos, experiments show that the expression of the genes is remarkably up-regulated under the stress of salt, drought and abscisic acid (ABA), and after the genes are introduced into rice through a transgenic technology, the germination rate and the root growth capability of the rice under the stress of salt are remarkably improved. The invention provides key gene resources and technical support for molecular design and breeding of saline-alkaline tolerant crops.

The present application relates to the technical field of soil pollution treatment, in particular to a bamboo-based soda saline soil remediation agent and a preparation method thereof, the bamboo-based soda saline soil remediation agent is composed of the following components in parts by weight: 46-50 parts of mature bamboo-based organic matter, 31-36 parts of modified bamboo charcoal, 10-13 parts of potassium humate, 5-6 parts of desulfurized gypsum, and 1-3 parts of iron-zinc-manganese chelate; the mature bamboo-based organic matter is bamboo-based organic material treated by aerobic fermentation; the modified bamboo charcoal is obtained by modifying bamboo charcoal with citric acid and phytic acid, and has carboxyl and phosphoric acid functional groups grafted on the surface. The present application can significantly improve the physicochemical properties of soda saline soil, achieve the multiple goals of desalination, alkali reduction and soil fertilization by converting bamboo raw materials into mature bamboo-based organic matter and modified bamboo charcoal, and combining the synergistic effect of potassium humate, desulfurized gypsum and iron-zinc-manganese chelate.

The invention discloses a phyllostachys edulis sourced PeDHN2 gene and application of a coding protein of the phyllostachys edulis sourced PeDHN2 gene, and belongs to the technical field of plant biological breeding. The nucleotide sequence of the PeDHN2 gene is as shown in SEQ ID NO. 11; the sequence of the PeDHN2 gene coding protein is as shown in SEQ ID NO. 12. According to the PeDHN2 gene cloned from moso bamboos, experiments show that expression of the gene is remarkably up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after the gene is introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are remarkably improved. The invention provides key gene resources and technical support for molecular design and breeding of saline-alkaline tolerant crops.

The application discloses a Phyllostachys edulis PeSVP gene, a coding protein and application thereof, and belongs to the technical field of plant genetic engineering.The nucleotide sequence of the PeSVP gene disclosed by the application is shown as SEQ ID NO.1, and the amino acid sequence of the coding protein is shown as SEQ ID NO.2.The application constructs a PeSVP gene overexpression vector of Phyllostachys edulis, transforms the constructed PeSVP gene overexpression vector of Phyllostachys edulis into Arabidopsis thaliana, and cultivates, screens and obtains a transgenic Arabidopsis thaliana strain with delayed flowering time.The application discloses the function of the PeSVP gene for the first time, the transgenic Arabidopsis thaliana plant has delayed flowering time after the PeSVP gene is transformed into the Arabidopsis thaliana plant, shows an obvious late-flowering phenotype, and the number of rosette leaves is increased;the expression amount of a downstream regulation gene FT is significantly reduced.The application can be used in molecular breeding of plant flowering period regulation, and has important significance for cultivating new plant germplasm with higher biological yield.