108 results about "Phyllostachys edulis" patented technology

The invention discloses enterobacter ludwigii PN6 and application thereof. The classification name of the strain is Enterobacter ludwigii with a strain number of PN6, the strain is collected in the China Center for Type Culture Collection and has a collection number of CCTCC NO: M 2019881. The strain PN6 screened from a Phyllostachys edulis root system has an obvious anti-bacteria function for a pathogenic bacterium, i.e., C .phyllostachydis, of the shoot blight of Phyllostachys edulis, a fermentation liquor inhibition rate of the strain PN6 can be 49.28%, and in addition, the strain PN6 has acapacity of efficiently dissolving phosphorus and secreting IAA (indole-3-acetic acid). The strain PN6 disclosed by the invention has an acceleration function for the growth of Phyllostachys edulis,an experiment data displays that a liquid fungicide prepared from the PN6 has an obvious growth promotion function for growth indexes, including the ground diameter, the seedling height, the chlorophyll and the like of the Phyllostachys edulis, in addition, the stress resistance of the Phyllostachys edulis can be increased, and an occurrence probability of the shoot blight of Phyllostachys edulisis reduced. Therefore, the invention provides an excellent strain resource for developing an environmentally-friendly special biofertilizer for the Phyllostachys edulis.

The invention discloses a method for removing a bamboo stump from phyllostachys pubescens mazel and relates to a method for cultivating the phyllostachys pubescens mazel. The method is characterized by comprising the following steps of: punching holes on joints of the bamboo stump from the top to the bottom until 1 to 4 joints are formed between the bamboo stump and a bamboo root; putting an explosive detonator into a space chamber below the joint which is punched lastly; and detonating the bamboo stump. The method has the advantages that: the bamboo stump is split and is removed from underground to ground surface by a blasting method; and the main living tissues of the bamboo stump are basically damaged, so that the bamboo stump leaves an underground space for the growth of an underground bamboo root, the formation of a bamboo shoot and the growth of new bamboo.

The invention discloses a phyllostachys edulis protoplast culture method. The method comprises the steps of performing enzymolysis to embryonic calluses, performing filtration, centrifugal separation and purification to obtained protoplasts after the enzymolysis, then culturing the purified protoplasts on a proper culture medium to enable the protoplasts to regenerate cell walls to form embryonic calluses which germinate to form regenerated plants, finally performing seedling strengthening and hardening to the regenerated plants and then transplanting seedlings to survive. The phyllostachys edulis protoplast culture method can solve the technical problem of phyllostachys edulis protoplast culture, provides a technical platform for the cell engineering breeding of phyllostachys edulis, and also provides a reference for related researches on other bamboo plants.

The embodiment of the invention provides a moso bamboo forest distribution recognition method and device, equipment and a storage medium, and relates to the technical field of information recognition and extraction of remote sensing images. The recognition method comprises the following steps: S1, collecting remote sensing images of different phyllostachys pubescens leaf phenological periods in a target area; s2, calculating a plurality of vegetation indexes and a plurality of texture features of remote sensing images of different phyllostachys pubescens leaves in phenological periods; s3 and S4, selecting a first feature and a second feature from the plurality of vegetation indexes and the plurality of texture features; and S5, based on the first feature, calculating a plurality of phenological features of the target area. And S6, selecting a third feature from the plurality of phenological features. And S7, fusing the first feature, the second feature and the third feature of the remote sensing image through a convolution method to obtain a to-be-identified image of the target area, and recognizing the moso bamboo forest distribution condition in the to-be-identified image through the classification model. The distribution condition of the phyllostachys edulis forest is identified through phenological characteristics, and the classification precision of the phyllostachys edulis forest is greatly improved.

The invention discloses a phyllostachys edulis PeAPX5 gene and application. Sequences of the phyllostachys edulis PeAPX5 gene and encoded protein thereof are as shown in SEQ ID NO: 1 and SEQ ID NO: 2 respectively. The PeAPX5 gene is cloned from phyllostachys edulis for the first time, and the biological function of the PeAPX5 gene is verified by over-expressing the PeAPX5 gene in arabidopsis thaliana. The PeAPX5 gene has the function of regulating and controlling plant stress resistance, a powerful tool can be provided for phyllostachys edulis transgenic research, and valuable candidate genes can be provided for phyllostachys edulis resistance molecular breeding.

The invention relates to a thick bamboo tube grape wine manufacturing method which comprises the steps of selecting a thick bamboo tube and making grape wine. The step of selecting the thick bamboo tube is divided into selecting bamboo, drying, cleaning and punching; the step of making the grape wine is divided into breaking, separating, fermenting, secondarily fermenting and packaging; in bamboo selecting, moso bamboo which grows for 3 to 5 years and has a diameter of 12mm to 15mm is cut down in autumn, and in drying, the cut-down moso bamboo is cut into a thick bamboo tube containing two bamboo joints and a thick bamboo tube without a bamboo joint as quickly as possible; the thick bamboo tube without the bamboo joint and bamboo branches are washed clean and broken into bamboo particles with a side length smaller than 8mm, the bamboo particles are treated by two modes, one mode is that the bamboo particles are dried under 40 DEG C for 15 days, and the other mode is that the bamboo particles which are dried under 40 DEG C for 15 days are put into an oven to be dried under 120 DEG C for 2 days. The bamboo particles disclosed by the invention has the advantages of tight meat quality, unique flavor, high nutritional value and delicious beef taste; thus, the grape wine disclosed by the invention has the advantages of low alcohol content and rich nutrition.

The invention discloses a method for planting selenium-rich bletilla striata under phyllostachys edulis forests, and relates to the technical field of planting of traditional Chinese medicinal materials. The method for planting selenium-rich bletilla striata under phyllostachys edulis forests comprises the steps of land selection, soil preparation, bletilla striata planting, fertilizer application, intertillage weeding, topdressing, selenium-rich nutrient agent spraying, selenium fertilizer spraying, harvesting and the like. By means of the method, the artificial planting appropriateness of bletilla striata is enhanced, the survival rate reaches 98% or above, and the average yield per mu is 1277 kg and is highly than the current fresh product yield per mu of 800-1000 kg.

The invention discloses a method of planting dendrobium officinale by using moso bamboo. The method comprises the following steps: 1, material selection: selecting the moso bamboo, and longitudinally cutting the moso bamboo to form bamboo pieces; 2, construction of a planting layer: constructing the bamboo pieces to form the planting layer, punching through holes in the bamboo pieces located at the bottom of the planting layer, and forming a gap between the planting layer and the ground; 3, matrix filling: paving a matrix for planting the dendrobium officinale on the bottom of the planting layer; 4, planting: inserting dendrobium officinale seedlings into the matrix for planting the dendrobium officinale. The method is high in yield and easy for large-area popularization, and the produced dendrobium officinale has high quality.

The invention discloses a method for planting polygonatum odoratum under phyllostachys edulis forests, and relates to the technical field of planting of traditional Chinese medicinal materials. The method for planting polygonatum odoratum under phyllostachys edulis forests comprises the steps of land selection, soil preparation, planting of polygonatum odoratum rhizome, fertilization, intertillage weeding, topdressing, harvesting and the like. The problem that the planting cost is increased due to the fact that shading measures need to be manually adopted for planting polygonatum odoratum is solved, the occurrence rate of leaf spot diseases, root rot, grey speck disease and purple round disease is low, the survival rate reaches 98% or above, and the average yield per mu is 10500-11500 kg and is highly than the current yield per mu of 6000 kg.

The invention provides a method for manufacturing a high-strength composite furniture panel material by utilizing moso bamboo fibers, and relates to the field of furniture plates. The method comprisesthe following steps: drying and crushing wool, soaking the wool in a sodium hydroxide solution, carrying out cooking for 3-5 hours, and performing steam explosion to obtain the moso bamboo fibers; carrying out ultrasonic oscillation treatment on the moso bamboo fibers, disodium ethylene diamine tetraacetate and KH-550 for 5-10 minutes, adding nano calcium carbonate, carrying out mixing and stirring, and filtering out the moso bamboo fibers to obtain modified moso bamboo fibers; mixing the modified moso bamboo fiber with lactic acid to obtain a modified moso bamboo fiber/lactic acid graft; adding the modified phyllostachys edulis fiber/lactic acid graft, polycarbonate, polylactic acid, a maleic anhydride compatilizer and a butylene terephthalate copolymer into an open type mixing mill andcarrying out melt blending for 15 to 30 minutes at 140 to 160 DEG C, crushing the mixture to certain grain size after batch-out, and carrying out compression molding. The panel material is high in mechanical performance, is resistant to water and mildew, and can be applied to furniture building materials on a large scale.

The invention belongs to the technical field of forestry, and relates to a method for constructing a small and medium diameter class covering moso bamboo shoot-rhizoma corydalis imitation wild crop rotation system. The method includes the following steps that a moso bamboo forest is selected; the moso bamboo forest is optimized; a rhizoma corydalis planting matrix is prepared; rhizoma corydalis is planted; weeding is conducted; topdressing is conducted; irrigation and drainage treatment is conducted well in time; prevention and treatment on diseases and insect damage is conducted well in time; rhizoma corydalis is harvested in the first year, or not harvested in the first year and continues to be cultivated till May in the second year to be harvested. The moso bamboo forest is managed, it is unnecessary to take young growth tending measures such as weeding, soil loosening, fertilizing and drought resisting in special projects, and labor is saved; rhizoma corydalis planting is achieved, moso bamboo coverage and rehabilitation in the mode of bamboo shoots in spring germinate in winter are promoted, the use ratio of the moso bamboo forest is increased, and incomes of farmers are increased. In addition, during rhizoma corydalis interplanting, soil used for moso bamboo forest covering is used, heavy subsoil application is not needed, and planting cost is reduced.

The invention discloses a Phyllostachys edulis PeTCP10 protein capable of controlling leaf curling, and applications thereof, wherein the amino acid sequence of the Phyllostachys edulis PeTCP10 protein is represented by SEQ ID No.3. The invention further provides a gene sequence encoding the Phyllostachys edulis PeTCP10 protein, wherein the deoxynucleotide sequence of the Phyllostachys edulis PeTCP10 protein is represented by SEQ ID No.2. The invention further relates to a vector containing the deoxynucleotide sequence of the gene. According to the present invention, a recombinant overexpression vector pCAMBIA1301-35S-PeTCP10 is transfected into wild-type Arabidopsis thaliana by using an Agrobacterium-mediated inflorescence impregnation method, and the results show that the leaf curling degree of the transgenic plant over-expressing PeTCP10 is significantly higher than the leaf curling degree of the wild type plant, such that the transgenic Arabidopsis thaliana can produce significantleaf curling through the Phyllostachys edulis PeTCP10 gene.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a Phyllostachys edulis MYB transcription factor gene PeMYB103A, the nucleotide sequence of which is as shown in SEQ ID NO: 1. The invention further discloses application of the Phyllostachys edulis MYB transcription factor gene PeMYB103A in increasing the content of cellulose, the content of hemicellulose and the content of biomass and reducing the content of lignin. The Phyllostachys edulis MYB transcription factor gene PeMYB103A provided by the invention has a wide application prospect in directional breeding of plants, and a new gene resource is provided for plant genetic engineering.

The present invention provides phyllostachys edulis endophytic Enterobacter aerogenes with phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects and application thereof. The phyllostachys edulis endophytic Enterobacter aerogenes is Enterobacter aerogenes CT-B09-2 which is preserved in a general microorganism center of the China committee for culture collection of microorganismson July 3, 2017, and the preservation number is CGMCC No.14377. The phyllostachys edulis endophytic Enterobacter aerogenes has the phosphorus-dissolving, potassium-dissolving and nitrogen-fixation effects, can be colonized in plants and improve the enzyme activity, and has the important effects on regulating plant growth and development.

The invention discloses a method for improving carbon reserve of a phyllostachys edulis forest. The method comprises the following steps: adjusting density of standing phyllostachys edulis in a forest to be greater than or equal to 2,666 plants/hm<2>; adjusting a breast diameter of phyllostachys edulis in the phyllostachys edulis forest to be greater than or equal to 10.79 cm; controlling a proportion of 1-year-old phyllostachys edulis, 2-3-year-old phyllostachys edulis, 4-5-year-old phyllostachys edulis and 6-7-year-old phyllostachys edulis in the phyllostachys edulis forest to be 1:2:2:(0-1); and conducting operation treatment on the phyllostachys edulis forest in a growth process of the phyllostachys edulis forest, wherein the operation treatment comprises reclamation treatment and the reclamation treatment is that bamboo shoots are dug for reclamation once every 3-5 years. The method for improving carbon reserve of a phyllostachys edulis forest manages the phyllostachys edulis forest from multiple aspects of a breast diameter, a standing-bamboo density, an age structure and operation treatment of phyllostachys edulis, controls the carbon content of forest land soil while reasonably improving biomass of the phyllostachys edulis, and achieves a purpose of increasing a carbon sink of the forest.

The invention discloses a method for measuring and calculating diffusivity and key regulatory factors of a single phyllostachys edulis. The method comprises the steps of selecting a forest stand sample plot in which phyllostachys edulis is distributed; marking a single phyllostachys edulis in the sample plot, setting a sample circle after marking for two years, and calculating the diffusivity index of the single phyllostachys edulis; calculating stand structure indexes of the sample plot, wherein the stand structure indexes include the stand density, the average diameter at breast height, the average tree height, the species richness index, the Marglef richness index, the Shannon-Wiener diversity index, the Pielou uniformity index, the tree species simple mingling degree, the diversity mingling degree, the competition index and the aggregation index; classifying the diffusivity indexes of the single phyllostachys edulis as a group of variables, classifying the forest stand structure indexes as a group of variables, and obtaining the relation between the diffusivity of the single phyllostachys edulis and the key regulatory factors through canonical correlation analysis. Compared with an existing method for calculating the diffusivity of the single phyllostachys edulis by manually excavating underground bamboo rhizomes, the method is more labor-saving and time-saving, the efficiency is obviously improved, and the change and influence on the soil structure and microorganisms caused by excavating the bamboo rhizomes are avoided.

The invention discloses a phyllostachys edulis stand structure optimization method based on minimum entropy. The method comprises the following steps: respectively optimizing a phyllostachys edulis stand structure by adopting a constrained nonlinear model and a real stand structure adjustment mode; and comprising the optimization results of the two optimization modes. According to the constrainednonlinear model optimization mode, a minimum entropy breast-height diameter structure optimization model and an age structure optimization model are constructed, the models are optimized and solved, and an optimization result of the breast-height diameter structure and the age structure of the phyllostachys edulis forest stand is obtained; according to the real stand structure adjustment and optimization method, comprehensive structure adjustment is performed on phyllostachys edulis of each age level and each diameter level in a felling mode through the statistical table of age levels and diameter levels in combination with the actual situation of the phyllostachys edulis stands, and the optimization result of the diameter at breast-height and the age structure of the phyllostachys edulisstands is obtained. The method has the advantages that the structure of the phyllostachys edulis stand is optimized by using a constrained nonlinear model and a real stand structure adjustment mode, enlightenment is provided for the operation of the phyllostachys edulis stand, and the productivity of phyllostachys edulis is improved.

The invention provides a method for cultivating radix notoginseng in a bamboo stand. The method comprises the steps of bamboo stand selection, bamboo stand clearing, radix notoginseng seed treatment and seedling culture, planting, field management and harvesting. According to the method, the slope of the bamboo stand for radix notoginseng cultivation is 5-25 degrees, bamboo plantation density is controlled to be 15-200 pieces per mu, bamboo shoots are harvested in spring based on the principle of reserving the sparse and big and controlling the dense and small, 15-20 new bamboos are left, a sound radix notoginseng growth environment is guaranteed, the quality of radix notoginseng is improved, and green ecological organic radix notoginseng is cultivated; by cultivating radix notoginseng under a bamboo forest, forest land resources can be sufficiently utilized, farmland is not used, and radix notoginseng cultivation space is enlarged; meanwhile, radix notoginseng can be shaded by the bamboo forest, a good natural environment is provided, sun shelter building is not needed, and production cost is reduced greatly; besides, there is no weed under the bamboo forest, manual weeding is not needed, and therefore production cost can be further reduced.

The invention discloses a processing method of a bamboo shoot sheath circular tea tray. The processing method comprises the steps of firstly, selecting naturally shedding bamboo shoot sheaths, airing the bamboo shoot sheaths, stewing the aired bamboo shoot sheaths in boiling water to be softened, after flattening and shaping, putting the bamboo shoot sheaths into a roller to be rolled, removing thin burrs growing on the surfaces of the bamboo shoot sheaths, and manufacturing the bamboo shoot sheaths into triangular bamboo shoot sheath sheets through stamping of a punch; secondly, neatly placing the eight triangular bamboo shoot sheath sheets, and gluing a splicing joint of the two adjacent triangular bamboo shoot sheath sheets with a punched adhesive tape which is dipped in water, so as to obtain a circular bamboo shoot sheath spliced sheet; again, purchasing impregnation transparent wear resistant paper and formaldehyde-free soybean glue, adding an isocyanic acid tallow adhesive, after uniform mixing, brushing a mixture on the two surfaces of the circular bamboo shoot sheath spliced sheet adopted as a rubber plate, and carrying out assembly according to the specified requirement; and finally, brushing a release agent in a tea tray mold, putting the assembled circular bamboo shoot sheath spliced sheet in the tea tray mold, starting a hot press for hot pressing to obtain a bamboo shoot sheath circular tea tray blank, rounding the bamboo shoot sheath circular tea tray blank through a grinding machine, brushing boiled oil on the rounded bamboo shoot sheath circular tea tray blank, and airing to obtain the bamboo shoot sheath circular tea tray. According to the processing method, economic value of phyllostachys edulis can be greatly improved.

The invention belongs to the fields of molecular biology and genetic engineering, and particularly relates to a Gts (glycosyltransferases) key gene PeIRX10 taking part in xylan synthesis. The gene has the nucleotide sequence shown as SEQ ID No:1. The invention provides a powerful tool for knowing the formation of the secondary cell wall and illuminating the molecular basis of the fast growth of phyllostachys edulis tissues. The effects that the xylan synthesis defects in arabidopsis mutants can be overcome by using the PeIRX10, and the defects of the secondary cell wall in the arabidopsis mutants can be overcome in a complementary way are achieved.

The invention relates to the field of methods for planting Polygala fallax Hemsl., and provides a method for planting Polygala fallax Hemsl. under a phyllostachys edulis forest. The defects that Polygala fallax Hemsl. obtained through a planting method in the prior art is low in planting survival rate and yield, and the forest utilization rate is low are overcome. The method includes the followingsteps of forest selection; 2, forest cleaning and preparation; 3, seedling selection; 4, application of the planting technology; 5, hole expansion and weeding; 6, fertilization; 7, defloration; 8, plant disease and pest control; 9, harvesting and storage.