3 results about "Lignin biosynthesis" patented technology

The invention belongs to the technical field of agricultural biological genetic engineering, and particularly discloses application of a PbbHLH96 gene in regulation and control of pear fruit lignin synthesis and / or stone cell formation. According to the application of the PbHLH96 gene in regulation and control of lignin synthesis and / or stone cell formation of pear fruits, the nucleotide sequence of the PbHLH96 gene is as shown in SEQ ID NO. 1. The PbHLH96 gene is directly combined with a PbCAD6 promoter region to inhibit transcription of the PbHLH96 gene, so that biological synthesis of lignin of pear fruits is negatively regulated, and the PbHLH96 gene has a great application value for improving the quality of the pear fruits.

The invention provides application of a CsWRKY41 gene in regulation and control of plant lignin synthesis, and belongs to the technical field of plant genetic engineering. A plant overexpression vector and a gene silencing vector of the CsWRKY41 gene are constructed and are respectively subjected to genetic transformation in arabidopsis thaliana and a tea tree, and the result proves that the lignin content of a transgenic plant can be obviously improved by overexpressing the CsWRKY41 gene; on the contrary, silencing of the gene leads to reduction of lignin content. The invention discloses a molecular mechanism of the tea tree CsWRKY41 gene for enhancing plant drought resistance by positively regulating lignin biosynthesis for the first time. The gene and the related expression vector can be used for cultivating tea trees with high lignin content and strong drought resistance and even other new forest or crop varieties, and have wide application prospects in agricultural water conservation and stress resistance breeding.

The application belongs to the field of plant biotechnology, and specifically discloses a Punica granatum ABCG9 gene and application thereof. The PgABCG9 gene of the Punica granatum is cloned for the first time, and the nucleic acid sequence is shown as SEQ ID No. 1. The PgABCG9 is only expressed in the inner seed coat of the Punica granatum, and the transcription level is negatively correlated with the hardness of the Punica granatum seeds. Under the same growth conditions, the PgABCG9 transgenic Arabidopsis plants show slow growth, reduced lignin deposition, reduced expression level of lignin biosynthesis genes, and reduced content of metabolic products in the lignin biosynthesis pathway. Subcellular localization analysis shows that the PgABCG9 is located in the Golgi apparatus. The PgABCG9 plays a negative regulation role in the lignin biosynthesis, and can regulate the lignin synthesis of the inner seed coat of the Punica granatum, and adjust the kernel hardness, thereby laying a foundation for improving the fruit quality of the Punica granatum and other drupes from the direction of lignin in the future.