The application belongs to the field of
plant biotechnology, and specifically discloses a
Punica granatum ABCG9
gene and application thereof. The PgABCG9
gene of the
Punica granatum is cloned for the first time, and the
nucleic acid sequence is shown as SEQ ID No. 1. The PgABCG9 is only expressed in the inner seed coat of the
Punica granatum, and the transcription level is negatively correlated with the
hardness of the Punica granatum seeds. Under the same growth conditions, the PgABCG9 transgenic
Arabidopsis plants show
slow growth, reduced
lignin deposition, reduced expression level of
lignin biosynthesis genes, and reduced content of metabolic products in the
lignin biosynthesis pathway.
Subcellular localization analysis shows that the PgABCG9 is located in the
Golgi apparatus. The PgABCG9 plays a negative regulation role in the
lignin biosynthesis, and can regulate the
lignin synthesis of the inner seed coat of the Punica granatum, and adjust the kernel
hardness, thereby laying a foundation for improving the fruit quality of the Punica granatum and other drupes from the direction of lignin in the future.