Lignin synthesis related protein and coding gene and use thereof

A technology of related proteins and coding genes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of isolation and identification of genes that are not related to lignin, and achieve the effect of increasing value

Active Publication Date: 2009-04-22
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rice is the food crop with the largest planting area in my country, and its straw can be used for pulping and feed, but so far no lignin-related genes have been isolated and identified in rice

Method used

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  • Lignin synthesis related protein and coding gene and use thereof
  • Lignin synthesis related protein and coding gene and use thereof
  • Lignin synthesis related protein and coding gene and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Obtainment of lignin synthesis-related protein GH2 and its coding gene

[0041] 1. Phenotype analysis of rice golden glume mutant gh2

[0042] The rice golden glume mutant gh2 is a near allelic line of ZF802. Compared with the control ZF802, the phenotype of golden glumes is brown-red on the leaf sheaths and stems from the jointing stage, and the glumes are also brown-red at the heading stage, and the brown-red color gradually changes as the rice matures. Pale, turning brownish-yellow at maturity. The mutant phenotype does not affect the normal growth and development of rice under normal cultivation conditions ( figure 1 ). figure 1 Among them, a is the panicle of the control ZF802, green; b is the panicle of the golden glume mutant gh-2, reddish brown; c is the stem of the control ZF802, green; d is the stem of the golden glume mutant gh2, reddish brown; The seeds of the control ZF802 are light yellow; f is the seed of the golden glume mutant gh2 is brow...

Embodiment 2

[0071] Embodiment 2, Complementation experiment of the gh2 phenotype of the golden glume mutant

[0072] 1. Construction of the expression vector pCGH containing the genomic gene coding sequence of GH2 whose 3465th deoxynucleotide at the 5' end of sequence 3 is G

[0073] BAC OJ1145_F01 (purchased from the Institute of Genetics and Development, Chinese Academy of Sciences) was digested with BamHI and HindIII to obtain a complete DNA sequence containing 2,397 bases upstream of the start codon ATG of GH2 and 2460 bases after the stop codon TGA. The DNA fragment of the long sequence was cloned between the BamHI and HindIII recognition sites of pCAMBIA1300 (CAMBIA, Canberra, Australia), and the genome gene of GH2 containing G at the 3465th deoxynucleotide at the 5' end of sequence 3 was constructed. The expression vector pCGH (3585bp in total) of the coding sequence ( Figure 4 ).

[0074] 2. Construction of the expression vector pCGHS containing the 3465th position from the 5' ...

Embodiment 3

[0084] Embodiment 3, classic hybridization technique utilizes GH2 gene

[0085] The male parent of the golden glume mutant gh2 was crossed with the recipient plant ZF802 as the female parent, and then the recipient plant ZF802 was repeatedly backcrossed 10 times to transfer the phenotype of the golden glume mutant gh2 to the recipient plant, and the GH2 A genetically modified plant having such figure 1 The gh2 phenotype of the aureus mutant shown.

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Abstract

The invention discloses a lignin-related protein as well as its gene encoding and application. The lignin-associated protein has one of the following amino acid residues sequences: (1) SEQ ID No. : 2 in the sequence table, and (2) lignin-related protein after SEQ ID No. : 2 one or several amino acid residues replaced and / or deleted and / or added in the sequence table. The lignin-related protein gene can regulate the content and composition of rice lignin without influences on plant growth and development, and improve the values as feed and industrial raw material of paper. In addition, the lignin-related protein gene also has important theoretical value in revealing the mechanism of plant lignin biosynthesis.

Description

technical field [0001] The invention relates to a lignin synthesis-related protein, its coding gene and application in the field of plant genetic engineering. Background technique [0002] Lignin is a kind of polyphenolic biopolymer whose content in plant cell wall is second only to cellulose. Lignin can improve the strength of cell wall by cross-linking with carbohydrates such as cellulose hemicellulose, and improve the resistance of plants to diseases and insect pests. Lignin participates in the formation of conduits and sieves, so that water and solutes can be transported through the vascular system (MalcolmM. Campbell and Ronald R. Sederoff. Variation in lignin content and composition. Plant physiol, 1996, 110: 3-13). Lignin is mainly derived from complex racemic aromatic heteropolymers of three hydroxycinnamyl alcohol monomers—p-coumaryl M1H, coniferyl M1G, and sinapyl M1S (Wout Boerjan, John Ralph, and Marie Baucher. Lignin biosynthesis. Annu. Rev. Plant Biol. 2003. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82A01H4/00
Inventor 程祝宽钱前张可伟李明
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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