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A kind of Cedar cfccr gene and its application

A cedar and gene technology, applied in the fields of plant molecular biology and genetic engineering, can solve problems such as abnormal plant development, lower lignin content, and loose secondary wall structure, so as to improve plant stress resistance and increase wood density , improve the quality and quality of the effect

Active Publication Date: 2020-12-22
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that: the faster the inhibition of CCR activity decreases, the more the corresponding lignin content decreases, which affects the growth and development of plants
Inhibiting CCR activity will reduce lignin content, reduce secondary wall deposition, and relax secondary wall structure. In severe cases, it will cause abnormal plant development

Method used

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  • A kind of Cedar cfccr gene and its application
  • A kind of Cedar cfccr gene and its application
  • A kind of Cedar cfccr gene and its application

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Experimental program
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Effect test

Embodiment 1

[0028] 1. Cloning and expression analysis of Cryptomeria CfCCR gene

[0029] 1. Cloning of Cryptomeria CfCCR gene

[0030] Select Cedar cedar growing well in the Arboretum of Nanjing Forestry University, remove the epidermis, scrape the vascular tissue with a blade, extract total RNA and reverse transcribe it into cDNA. According to the conserved sequence analysis data of the CCR gene of Chinese fir and the differentially expressed genes analyzed by the high-throughput sequencing data of the Cedar transcriptome in our laboratory, a CCR homologous sequence was screened out using the NCBI online tool Blast, and primers were designed.

[0031] Design specific amplification primers CCR-F and CCR-R, carry out PCR amplification with cDNA as template, the recovery of PCR product, connect pBI121 carrier ( figure 1 ).

[0032] The specific amplification primers CCR-F and CCR-R are:

[0033] CCR-F: 5′-TAATTGTTGTAAATACGCTTG-3′

[0034] CCR-R: 5′-AATTACAGTTCTTATTGGCAT-3′

[0035] The...

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Abstract

The invention discloses a cedar CfCCR gene and application thereof, and belongs to the technical fields of plant molecular biology and genetic engineering. The cedar lignin synthesis regulating CfCCR gene of the present invention has the nucleotide sequence shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. The CfCCR gene is the key rate-limiting enzyme in the specific pathway of lignin synthesis. It regulates the expression of genes related to lignin synthesis in plants by encoding proteins, and plays an important role in lignin biosynthesis and stress resistance. Tobacco transgenic for CfCCR gene had increased expression of lignin and well-developed cell wall. Therefore, the thickness of the xylem cell wall in cedar or other plant stems can be changed by expressing the CfCCR gene, thereby increasing the wood density, improving the quality and quality of the wood and the stress resistance of the plant.

Description

technical field [0001] The invention belongs to the technical fields of plant molecular biology and genetic engineering, and specifically relates to a cedar CfCCR gene and its application Background technique [0002] Cryptomeria fortunei (Cryptomeria fortunei) belongs to the Taxodiaceae (Taxodiaceae) Cryptomeria (CryptomeriaD.Don) plant, also known as the long-leaved peacock pine, tree, conical crown; bark reddish brown, fibrous, large branches in whorls, twigs thin Long, often drooping, with subulate leaves slightly curved inwards, curved inwards at the apex, and stomatal lines on the four sides. It is a unique tree species in my country and an important timber species. Its wood has a wide range of value and can also be used as ornamental plants and medicinals. The degree of lignification of cedar will affect the density of cedar wood, thus affecting the quality of cedar. [0003] CCR plays a key role in the specific pathway of lignin synthesis. It is the first key enzyme ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/84C12N1/21A01H5/00A01H6/82
CPCC12N9/0008C12N15/8255C12Y102/01044
Inventor 徐进华慧胡海亮郭臻昊杨俊杰
Owner NANJING FORESTRY UNIV
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