IbLEA14 gene from ipomoea batatas roots and use thereof

A gene and amino acid technology, applied in the field of IbLEA14 gene from sweet potato rhizome and its application, can solve the problem of lack of LEA14 gene, etc., and achieve the effect of increasing lignin content

Inactive Publication Date: 2011-10-05
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report of LEA14 gene in sweet potato

Method used

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  • IbLEA14 gene from ipomoea batatas roots and use thereof
  • IbLEA14 gene from ipomoea batatas roots and use thereof
  • IbLEA14 gene from ipomoea batatas roots and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Cloning, base sequence analysis and class relationship analysis of sweet potato IbLEA14 gene

[0075] Sweet potato (Ipomoea batatas (L.) Lam. cv. White star) plants were cultured in MS (Murashige and Skoog, 1962) basal medium for 60 days. Sweet potato plants cultured in MS basal medium were cultured in a 25°C thermostat for 60 days with a 16-hour light and 8-hour dark cycle, and then transferred to soil. The sweet potatoes transferred to the soil were used in the experiment after 14 days of acclimatization in a constant temperature machine at 25°C under the conditions of a 16-hour light and an 8-hour dark cycle. The fibrous roots of the cultivated plants were separated from the soil, and then dried in a constant temperature machine at 25° C. for 6 hours. After drying, total RNA was isolated from fibrous root tissue by the CTAB method (Kim et al. BMB Rep 42, 271-276, 2009). The mRNA was isolated using the Poly(A)Tract mRNA Isolation System, and the cDNA libr...

Embodiment 2

[0078] Example 2: Expression Analysis of IbLEA14 Gene in Sweet Potato Tissues

[0079]In order to analyze the expression state of each tissue of sweet potato IbLEA14 isolated from dried sweet potato rhizomes, RT-PCR and real-time fluorescent quantitative PCR were carried out. After isolating all RNA from sweet potato tissues (leaves, stems, tubers, fibrous roots and thick roots) using the CTAB method (Kim and Hamada, Biotech Lett 27, 1841-1845, 2005), use 2 μg of RNA to pass through MMLV Reverse Transcription System cDNA Synthesis kit (Promega Company) synthesized cDNA. The expression phenomenon of the IbLEA14 gene was investigated using the IbLEA14 gene-specific catalyst and the Accel Taq Premix kit (Gendocs). For the specific catalyst for IbLEA14, forward direction (5'-GCCCTGGATGTGGCAGTG-3'; SEQ ID NO: 3) and reverse direction (5'-GGCAGCTTCTGCCTCTGCTTC-3'; SEQ ID NO: 4) catalysts were used. As a result, the IbLEA14 gene was strongly expressed in fibrous roots and thick pig...

Embodiment 3

[0080] Example 3: Analysis of the expression characteristics of the IbLEA14 gene according to various stress treatments including drying treatment

[0081] In order to analyze the response degree of IbLEA14 gene to various stress treatments including desiccation, after treating the compounds related to various stress conditions, namely desiccation, phytohormones (ABA), low temperature and stress, real-time fluorescent quantitative PCR was used to analyze the Expression changes of the IbLEA14 gene.

[0082] First, in order to analyze the degree of response of the IbLEA14 gene to drying treatment, after drying the leaves and fibrous root tissues of sweet potato according to the time period (0, 1, 2, 8, 16 and 24 hours), the method implemented in Example 2 RNA was isolated and cDNA was synthesized separately thereafter. Real-time fluorescent quantitative PCR was performed with the IbLEA14 gene-specific catalyst used in Example 2. As a result, the IbLEA14 gene showed the stronge...

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Abstract

The invention relates to an IbLEA 14 (Ipomoea batatas Late embryogenesis abundant 14) protein related to the stress resistance of ipomoea batatas or the lignin biosynthesis. The invention further relates to a method for improving the stress resistance of plants or the lignin biosynthesis of plants through form quality conversion of recombinant vectors included in the protein genes in plant cells after the protein genes are coded and the a host cell is generated from the form quality conversion of the recombinant vectors. Further included is a method for making form quality conversing plant bodies with enhanced stress resistance or improved lignin biosynthesis by means of the recombinant vectors for form quality conversion of biological cells. The invention further relates to biologic energy obtained by the form quality conversing plant bodies provided with increased lignin biosynthesis and made by the above-mentioned method and the composition for improving the stress resistance or the lignin biosynthesis of the plant bodies having the genes.

Description

technical field [0001] The present invention relates to the IbLEA14 gene from the sweet potato rhizome and its use, and more specifically, to the IbLEA14 (Ipomoea batatas Late embryogenesis abundant 14) protein related to the stress resistance or lignin biosynthesis of the sweet potato, the gene encoding the protein, comprising The recombinant vector of the gene, the host cell transformed from the recombinant vector, the method of making the recombinant vector perform the transformation of shape and quality in the plant cell to improve the stress resistance of the plant or the lignin biosynthesis of the plant A method for manufacturing a stress-resistant form-transformed plant body or a form-transformed plant body with enhanced lignin biosynthesis comprising the step of using the recombinant vector to transform biological cells, from the enhanced plant produced according to the method The biological energy obtained by the shape-to-matter conversion plant body of lignin biosynt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/21C12N1/19C12N5/10C12N15/82A01H5/00C10L1/02C10L3/06C10L5/44
CPCY02E50/30Y02E50/10
Inventor 郭尚洙李幸顺郑在哲金闰熙朴成喆
Owner KOREA RES INST OF BIOSCI & BIOTECH
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